Abstract:
:NSP5 (NS26), the product of rotavirus gene 11, is a phosphoprotein whose role in the virus replication cycle is unknown. To gain further insight into its function, we obtained monoclonal antibodies against the baculovirus-expressed protein. By immunoprecipitation and immunoblotting experiments, we showed that (i) NSP5 appears in many different phosphorylated forms in rotavirus-infected cells; (ii) immunoprecipitated NSP5 from rotavirus-infected cells can be phosphorylated in vitro by incubation with ATP; (iii) NSP5, produced either by transient transfection of rotavirus gene 11 or by infection by gene 11 recombinant vaccinia virus or baculovirus, can be phosphorylated in vivo and in vitro; (iv) NSP5 expressed in Escherichia coli is phosphorylated in vitro, and thus NSP5 is a potential protein kinase; and (v) NSP5 forms dimers and interacts with NSP2. The intracellular localization of NSP5 in the course of rotavirus infection and after transient expression in COS7 cells has also been investigated. In rotavirus-infected cells, NSP5 is localized in viroplasms, but it is widespread throughout the cytoplasm of transfected COS7 cells. NSP5 produced by transfected COS7 cells did not acquire the multiphosphorylated forms observed in rotavirus-infected COS7 cells. Thus, there is a tight correlation between the localization of NSP5 in the viroplasms and its protein kinase activity in vivo or in vitro. Our results suggest that cellular or viral cofactors are indispensable to fully phosphorylate NSP5 and to reach its intracellular localization.
journal_name
J Viroljournal_title
Journal of virologyauthors
Poncet D,Lindenbaum P,L'Haridon R,Cohen Jdoi
10.1128/JVI.71.1.34-41.1997subject
Has Abstractpub_date
1997-01-01 00:00:00pages
34-41issue
1eissn
0022-538Xissn
1098-5514journal_volume
71pub_type
杂志文章abstract::The integrase protein of human immunodeficiency virus type 1 removes two nucleotides from the 3' ends of reverse-transcribed human immunodeficiency virus type 1 DNA (3' processing) and covalently inserts the processed ends into a target DNA (DNA strand transfer). Mutant integrase proteins that lack the amino-and/or ca...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.5911-5917.1994
更新日期:1994-09-01 00:00:00
abstract::RNA-dependent RNA polymerases were found to be associated with two salmonid rhabdoviruses: infectious hematopoietic necrosis (IHN) virus and the virus of hemorrhagic septicemia (VHS). The protein composition of these rhabdoviruses closely resembles that of rabies virus rather than that of vesicular stomatitis virus (M...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.22.3.839-843.1977
更新日期:1977-06-01 00:00:00
abstract:UNLABELLED:Testudinid herpesvirus 3 (TeHV-3) is the causative agent of a lethal disease affecting several tortoise species. The threat that this virus poses to endangered animals is focusing efforts on characterizing its properties, in order to enable the development of prophylactic methods. We have sequenced the genom...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01794-15
更新日期:2015-11-01 00:00:00
abstract::We have generated four mouse monoclonal antibodies (MAbs) to bovine papillomavirus virions that bound type-specific, adjacent, and conformationally dependent epitopes on the L1 major capsid protein. All four MAbs were neutralizing at ratios of 1 MAb molecule per 5 to 25 L1 molecules, but only three effectively blocked...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.11.7570-7574.1994
更新日期:1994-11-01 00:00:00
abstract::Small ruminants infected with peste des petits ruminants virus exhibit lesions typical of epithelial infection and necrosis. However, the only established host receptor for this virus is the immune cell marker signaling lymphocyte activation molecule (SLAM). We have confirmed that the ovine Nectin-4 protein, when over...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02792-12
更新日期:2013-04-01 00:00:00
abstract::As potential targets for human immunodeficiency virus type 1 and simian immunodeficiency virus (HIV-1 and SIV), dendritic cells (DCs) likely play a significant role in the onset and spread of infection as well as in the induction of antiviral immunity. Using the SIV-macaque system to study the very early events in DC-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.6.2936-2951.2002
更新日期:2002-03-01 00:00:00
abstract::The C-type lectins DC-SIGN and DC-SIGNR [collectively referred to as DC-SIGN(R)] bind and transmit human immunodeficiency virus (HIV) and simian immunodeficiency virus to T cells via the viral envelope glycoprotein (Env). Other viruses containing heavily glycosylated glycoproteins (GPs) fail to interact with DC-SIGN(R...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.2.1337-1346.2003
更新日期:2003-01-01 00:00:00
abstract::The structures of canine parvovirus (CPV) and feline parvovirus (FPV) complexed with antibody fragments from eight different neutralizing monoclonal antibodies were determined by cryo-electron microscopy (cryoEM) reconstruction to resolutions varying from 8.5 to 18 A. The crystal structure of one of the Fab molecules ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02532-08
更新日期:2009-06-01 00:00:00
abstract::The promoter P7535 of human papillomavirus type 8 and the promoter P7185 of bovine papillomavirus type 1 are negatively regulated by viral E2 proteins via the promoter proximal binding sites P2 and BS1, respectively. Mutations of these E2 binding sites can reduce basal promoter activity. This suggests binding of a tra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.10.8029-8034.1997
更新日期:1997-10-01 00:00:00
abstract::Following its entry into cells, pseudorabies virus (PRV) utilizes microtubules to deliver its nucleocapsid to the nucleus. Previous studies have shown that PRV VP1/2 is an effector of dynein-mediated capsid transport. However, the mechanism of PRV for recruiting microtubule motor proteins for successful neuroinvasion ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02052-18
更新日期:2019-03-21 00:00:00
abstract::Murine gammaherpesvirus 68 (MHV68) infection of mice provides a tractable small-animal system for assessing viral requirements for establishment of and reactivation from latency. The M2 gene product has no homology to any known proteins but has been shown to play a role in both the establishment of MHV68 latency and r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00651-08
更新日期:2008-08-01 00:00:00
abstract::Rous sarcoma virus (RSV), a simple retrovirus, needs to export unspliced viral RNA from the nucleus to the cytoplasm, circumventing the host cell restriction on cytoplasmic expression of intron-containing RNA. The cytoplasmic accumulation of full-length viral RNA is promoted by two cis-acting direct repeat (DR) elemen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.20.9507-9514.2000
更新日期:2000-10-01 00:00:00
abstract::Although monkey B virus (herpesvirus simiae; BV) is common in all macaque species, fatal human infections appear to be associated with exposure to rhesus macaques (Macaca mulatta), suggesting that BV isolates from rhesus monkeys may be more lethal to nonmacaques than are BV strains indigenous to other macaque species....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.9224-9232.1998
更新日期:1998-11-01 00:00:00
abstract::Infection with human T-cell leukemia virus type 1 (HTLV-1) can result in the development of HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP), a chronic inflammatory disease of the central nervous system (CNS). HTLV-2 is highly related to HTLV-1 at the genetic level and shares a high degree of sequen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01513-06
更新日期:2007-02-01 00:00:00
abstract::The parameters governing the activity of the cloned T4 gene 23, which codes for the major T4 head protein, were analyzed. Suppressor-negative bacteria carrying wild-type T4 gene 23 cloned into plasmid pCR1 or pBR322 were infected with T4 gene 23 amber phage also carrying mutations in the following genes: (i) denA and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.1.46-59.1981
更新日期:1981-07-01 00:00:00
abstract::Drug resistance remains a major concern for human immunodeficiency virus (HIV) treatment. To date, very few resistance mutations have emerged in first-line combination therapy that includes the integrase strand transfer inhibitor (INSTI) dolutegravir (DTG). In vitro, DTG selects for several primary mutations that indu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01189-18
更新日期:2019-01-04 00:00:00
abstract::We have purified the seven virus-specific RNAs which were previously shown to be induced in Sac(-) cells upon infection with mouse hepatitis virus strain A59 (W. J. M. Spaan, P. J. M. Rottier, M. C. Horzinek, and B. A. M. van der Zeijst, Virology 108:424-434, 1981). The individual RNAs, prepared by agarose gel electro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.1.20-26.1981
更新日期:1981-04-01 00:00:00
abstract::Simian immunodeficiency virus (SIV) of macaques isolate SIVmac239 is highly resistant to neutralization by polyclonal antisera or monoclonal antibodies, a property that it shares with most primary isolates of human immunodeficiency virus type 1 (HIV-1). This resistance is important for the ability of the virus to pers...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.18.9993-10003.2003
更新日期:2003-09-01 00:00:00
abstract::The DNA sequence of 3,240 nucleotides of the XbaI G fragment located in the unique long (UL) region of the equine herpesvirus 1 genome revealed two major open reading frames (ORFs) designated UL3 and UL4. The UL3 ORF of 470 amino acids (aa) maps at nucleotides (nt) 4450 to 3038 from the long terminus, and its predicte...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.9.5363-5372.1992
更新日期:1992-09-01 00:00:00
abstract::The chimpanzee monoclonal antibody (MAb) 5H2 is specific for dengue virus type 4 (DENV-4) and neutralizes the virus at a high titer in vitro. The epitope detected by the antibody was mapped by sequencing neutralization escape variants of the virus. One variant contained a Lys174-Glu substitution and another contained ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01420-07
更新日期:2007-12-01 00:00:00
abstract::Viral infectivity factor (Vif) is required for lentivirus fitness and pathogenicity, except in equine infectious anemia virus (EIAV). Vif enhances viral infectivity by a Cullin5-Elongin B/C E3 complex to inactivate the host restriction factor APOBEC3. Core-binding factor subunit beta (CBF-β) is a cell factor that was ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01924-14
更新日期:2014-10-01 00:00:00
abstract::Regulatory elements interacting with DNA-binding proteins have been investigated in the promoter sequence of the early PE38 gene in the Autographa californica nuclear polyhedrosis virus (AcNPV). A GATA motif located 50 nucleotides upstream of the PE38 transcriptional start site is recognized differentially in the cour...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.6.3494-3503.1992
更新日期:1992-06-01 00:00:00
abstract::The promoter of the major immediate-early (MIE) genes of human cytomegalovirus (HCMV), also referred to as the CMV promoter, possesses a cis-acting element positioned downstream of the TATA box between positions -14 and -1 relative to the transcription start site (+1). We determined the role of the cis-acting element ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01593-07
更新日期:2008-01-01 00:00:00
abstract:UNLABELLED:While numerous viral microRNAs (miRNAs) expressed by DNA viruses, especially herpesvirus family members, have been reported, there have been very few reports of miRNAs derived from RNA viruses. Here we describe three miRNAs expressed by bovine foamy virus (BFV), a member of the spumavirus subfamily of retrov...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03587-13
更新日期:2014-05-01 00:00:00
abstract::Two types of empty capsid particles that differed with respect to the presence of the two outer shell proteins were isolated from MA-104 cells infected with bovine rotavirus V1005. Three previously uncharacterized polypeptides, I, II, and III, migrating between VP2 and VP6, were detected in empty capsids but not in si...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.8.3635-3642.1990
更新日期:1990-08-01 00:00:00
abstract::Although several virologic and immunologic factors associated with an increased risk of perinatal human immunodeficiency virus type 1 (HIV-1) transmission have been described, the mechanism of mother-to-child transmission is still unclear. More specifically, the question of whether selective pressures influence the tr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.5.3050-3057.2003
更新日期:2003-03-01 00:00:00
abstract::The current studies demonstrate complex and seemingly contradictory effects by gamma interferon (IFN-gamma) on Friend virus (FV) infection. Both temporal and tissue-specific effects were observed. During the first week of infection, IFN-gamma-deficiency caused increased levels of FV infection in multiple tissues. Surp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.5.2225-2232.2002
更新日期:2002-03-01 00:00:00
abstract::The impact of antiretroviral therapy (ART) on the genetics of simian immunodeficiency virus (SIV) or human immunodeficiency virus (HIV) populations has been incompletely characterized. We analyzed SIV genetic variation before, during, and after ART in a macaque model. Six pigtail macaques were infected with an SIV/HIV...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01701-10
更新日期:2011-01-01 00:00:00
abstract::Massive infection of memory CD4 T cells is a hallmark of early simian immunodeficiency virus (SIV) infection, with viral infection peaking at day 10 postinfection (p.i.), when a majority of memory CD4 T cells in mucosal and peripheral tissues are infected. It is not clear if mononuclear cells from the monocyte and mac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06372-11
更新日期:2012-01-01 00:00:00
abstract::The VP7 and VP4 genes of seven antigenic mutants of simian rotavirus SA11 4fM (serotype 3) selected after 39 passages in the presence of SA11 4fM hyperimmune antiserum, were sequenced. Nucleotide sequence analysis indicated the following. (i) Twice as many amino acid substitutions occurred in the VP7 protein than in V...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.9.4534-4539.1990
更新日期:1990-09-01 00:00:00