Abstract:
:The VP7 and VP4 genes of seven antigenic mutants of simian rotavirus SA11 4fM (serotype 3) selected after 39 passages in the presence of SA11 4fM hyperimmune antiserum, were sequenced. Nucleotide sequence analysis indicated the following. (i) Twice as many amino acid substitutions occurred in the VP7 protein than in VP4, which has a molecular weight twice that of VP7. (ii) Most amino acid changes that occurred clustered in six variable regions of VP7 and in two variable regions of VP4; these variable regions may represent immunodominant epitopes. (iii) Most amino acid substitutions that occurred in VP7 and VP4 of these mutants were also observed in antigenic mutants selected with neutralizing monoclonal antibodies (NMAbs); however, some amino acid substitutions occurred that were not selected for NMAbs. (iv) On VP7, some of the neutralization epitopes appeared to be interrelated because amino acid substitution in one site affected binding of specific NMAbs to other sites, while other neutralization epitopes on VP7 appeared to be independent, in that amino acid substitution in one site did not affect the binding of NMAbs to another distant site.
journal_name
J Viroljournal_title
Journal of virologyauthors
Gorziglia M,Larralde G,Ward RLdoi
10.1128/JVI.64.9.4534-4539.1990subject
Has Abstractpub_date
1990-09-01 00:00:00pages
4534-9issue
9eissn
0022-538Xissn
1098-5514journal_volume
64pub_type
杂志文章abstract::The utility of the present generation of adenovirus (Ad) vectors for gene therapy applications could be improved by restricting native viral tropism to selected cell types. In order to achieve modification of Ad tropism, we proposed to exploit a minor component of viral capsid, protein IX (pIX), for genetic incorporat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.14.6893-6899.2002
更新日期:2002-07-01 00:00:00
abstract::Purified retrovirus Gag proteins can assemble in vitro into virus-like particles (VLPs) in the presence of RNA. It was shown previously that a Rous sarcoma virus Gag protein missing only the protease domain forms spherical particles resembling immature virions lacking a membrane but that a similar protein missing the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.21.10260-10268.2000
更新日期:2000-11-01 00:00:00
abstract::To determine which sequences or structures in the poliovirus 5' noncoding region (5'NCR) are involved in binding proteins used for internal ribosome binding and protein synthesis initiation, translation competition assays were performed in rabbit reticulocyte lysates in the presence and absence of HeLa cell extract. T...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.5.3101-3109.1992
更新日期:1992-05-01 00:00:00
abstract::The product of the human cytomegalovirus (HCMV) UL71 gene is conserved throughout the herpesvirus family. During HCMV infection, protein pUL71 is required for efficient virion egress and is involved in the final steps of secondary envelopment leading to infectious viral particles. We found strong indications for oligo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06556-11
更新日期:2012-03-01 00:00:00
abstract::A marked virus-inhibiting potency is obtained in the serum after intraperitoneal injection of polyacrylic acid (PAA) and polymethacrylic acid (PMAA) in mice. Much higher antiviral levels were reached than for other related polymers including dextran sulfate, heparin, polyvinyl sulfate, pyran copolymer, polystyrene sul...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.9.886-893.1968
更新日期:1968-09-01 00:00:00
abstract::Nucleocapsid assembly in hepadnavirus replication requires selective encapsidation of the pregenomic RNA template and the viral polymerase by the core proteins. It has been shown that an encapsidation signal located at the 5' end of the pregenomic RNA is responsible for its interaction with the polymerase. In the pres...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.8.5035-5042.1996
更新日期:1996-08-01 00:00:00
abstract::Escherichia coli K-12 wild type and a uvrA mutant derivative were used to construct isogenic strains bearing one, two, three, or more phage lambda cI genomes and containing increasing concentration of lambda repressor as measured by in vitro operator DNA-binding assays. The survival and phage induction in response to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.3.595-602.1978
更新日期:1978-06-01 00:00:00
abstract::A hallmark of infection with herpes simplex virus type 1 (HSV-1) is the establishment of latency in ganglia of the infected individual. During the life of the latently infected individual, the virus can occasionally reactivate, travel back to the eye, and cause recurrent disease. Indeed, a major cause of corneal scarr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02234-08
更新日期:2009-03-01 00:00:00
abstract::A retrovirus vector has been developed that selects for instances in which the provirus integrates in close proximity to cellular promoters. Coding sequences for a selectable marker (histidinol dehydrogenase) were inserted into the 3' long terminal repeat (LTR) of an enhancerless Molony murine leukemia virus. Although...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.8.3227-3233.1989
更新日期:1989-08-01 00:00:00
abstract::A/chicken/FJ/G9/09 (FJ/G9) is an H9N2 subtype avian influenza virus (H9N2 AIV) strain causing high morbidity that was isolated from broilers in Fujian Province of China in 2009. FJ/G9 has been used as the vaccine strain against H9N2 AIV infection in Fujian Province of China. Here, we report the complete genome sequenc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01623-12
更新日期:2012-09-01 00:00:00
abstract::The efficient attachment of pseudorabies virus to cultured cells is dependent on an electrostatic interaction between negatively charged cell surface heparan sulfate and the viral envelope glycoprotein gC. Deletion of the first one-third of gC severely impairs virus attachment, but the mutant virions are still capable...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.834-839.1995
更新日期:1995-02-01 00:00:00
abstract::Skin fibroblast cultures were established from eight individuals. These cell cultures, together with WI-38 cells, were examined for susceptibility to transformation by SV40 virus. Four transformation-susceptible cell lines (TS), established from patients with Down's syndrome, were found to be three to four times more ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.5.3.293-298.1970
更新日期:1970-03-01 00:00:00
abstract::Upon cell entry, the genomes of herpes simplex virus type 1 (HSV-1) and adenovirus (Ad) associate with distinct nuclear structures termed ND10 or promyelocytic leukemia (PML) nuclear bodies (NBs). PML NB morphology is altered or disrupted by specific viral proteins as replication proceeds. We examined whether adeno-as...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.1.389-398.2004
更新日期:2004-01-01 00:00:00
abstract::Specific cleavage of BK virus (MM) DNA with restriction endonuclease MboI gives rise to 10 fragments. Two techniques were used to determine the location of these fragments on the viral genome with respect to the three known sites for HindIII cleavage. In the first method, reciprocal digestion, individual MboI fragment...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.27.3.700-712.1978
更新日期:1978-09-01 00:00:00
abstract::To analyze intrauterine transmission, MT-2 cells, a human T-cell line producing human T-cell leukemia virus type I (HTLV-I), were injected into eight pregnant F344 rats, and cesarean section was performed at day 23 of pregnancy. HTLV-I provirus was detected by PCR in the liver and spleen taken from one of the eight fe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.1302-1305.1995
更新日期:1995-02-01 00:00:00
abstract::Single-envelope human immunodeficiency virus (HIV) vaccines have been studied for more than a decade, with some successes in homologous challenge experiments in nonhuman primates but with no clear successes in clinical trials. To gain insight into the breadth of the immunity elicited by such vaccines, we have dissecte...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.7.4231-4236.2003
更新日期:2003-04-01 00:00:00
abstract::The vaccinia virus H5 gene encodes a 22.3-kDa phosphoprotein that is expressed during both the early and late phases of viral gene expression. It is a major component of virosomes and has been implicated in viral transcription and, as a substrate of the B1 kinase, may participate in genome replication. To enable a gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.5.2393-2405.2000
更新日期:2000-03-01 00:00:00
abstract::Persistent hepatitis B virus (HBV) infection is characterized by a weak and narrowly focused CD8+ T-cell response to HBV that is thought to reflect the induction of central and/or peripheral tolerance to HBV proteins in neonatal and adult onset infections, respectively. Immunotherapeutic strategies that overcome toler...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.17.8609-8620.2002
更新日期:2002-09-01 00:00:00
abstract::Hepatitis C virus (HCV) nonstructural protein 4B (NS4B) is an integral membrane protein, which plays an important role in the organization and function of the HCV replication complex (RC). Although much is understood about its amphipathic N-terminal and C-terminal domains, we know very little about the role of the tra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02298-10
更新日期:2011-07-01 00:00:00
abstract::The Epstein-Barr virus oriP, when examined with the use of in vitro nucleosome reconstitution, was found to exhibit a partial sparing of nucleosome assembly within the 3' dyad. Analysis of the homologous region containing the origin of replication of herpesvirus papio disclosed that sparing of nucleosome assembly was ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.12.5505-5508.1989
更新日期:1989-12-01 00:00:00
abstract::An assay to identify interactions between Citrus Dwarfing Viroid (CDVd) and Citrus Tristeza Virus (CTV) showed that viroid titer was enhanced by the coinfecting CTV in Mexican lime but not in etrog citron. Since CTV encodes three RNA silencing suppressors (RSSs), p23, p20 and p25, an assay using transgenic Mexican lim...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02619-13
更新日期:2014-01-01 00:00:00
abstract::Streptomyces coelicolor is a model system for the study of Streptomyces, a genus of bacteria responsible for the production of many clinically important antibiotics. Here we report the genome sequence of ΦCAM, a new S. coelicolor generalized transducing bacteriophage, isolated from a soil sample originating from Linco...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02681-12
更新日期:2012-12-01 00:00:00
abstract::Varicella-zoster virus (VZV) is a herpesvirus and is the causative agent of chicken pox (varicella) and shingles (herpes zoster). Active immunization against varicella became possible with the development of live attenuated varicella vaccine. The Oka vaccine strain was isolated in Japan from a child who had typical va...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00777-08
更新日期:2008-11-01 00:00:00
abstract::In previous studies, we demonstrated that single-chain variable fragments (scFvs) from anti-human immunodeficiency virus (HIV) Env monoclonal antibodies act as entry inhibitors when tethered to the surface of target cells by a glycosyl-phosphatidylinositol (GPI) anchor. Interestingly, even if a virus escapes inhibitio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02080-17
更新日期:2018-03-14 00:00:00
abstract::The replication of the simian herpesvirus SA8 in Vero cells was examined. The time course of replication of the simian herpesvirus SA8 was found to be similar to that of the herpes simplex viruses. Infectious progeny virions were first detectable by 6 h postinfection and were readily released into the extracellular fl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.50.2.316-324.1984
更新日期:1984-05-01 00:00:00
abstract::The multiplicity of attachment (MOA) of a virion in any particular time interval is the average number of cellular attachment opportunities that must be blocked to keep the virion in suspension. MOA is usually proportional to incubation time and cell concentration. Low MOA (like low multiplicity of infection) is requi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.3.1782-1789.1994
更新日期:1994-03-01 00:00:00
abstract::Two regions of the hepatitis B virus (HBV) genome have been shown to display properties of a transcriptional enhancer. Enhancer 1 is active in most hepatoma lines examined as well as in some non-hepatocyte-derived cell lines. In contrast, enhancer 2 activity is strictly liver specific. In this study, we show that aden...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.12.7452-7460.1992
更新日期:1992-12-01 00:00:00
abstract::The assembly of human cytomegalovirus (HCMV) is thought to be similar to that which has been proposed for alphaherpesviruses and involve envelopment of tegumented subviral particles at the nuclear membrane followed by export from the cell by a poorly defined pathway. However, several studies have shown that at least t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.2.975-986.2000
更新日期:2000-01-01 00:00:00
abstract::The localization of the adenovirus type 5 34-kDa E4 and 55-kDa E1B proteins was determined in the absence of other adenovirus proteins. When expressed by transfection in human, monkey, hamster, rat, and mouse cell lines, the E1B protein was predominantly cytoplasmic and typically was excluded from the nucleus. When ex...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.6323-6335.1996
更新日期:1996-09-01 00:00:00
abstract::Borna disease virus (BDV) is a neurotropic virus with a broad host and geographic range. Lewis rats were immunized against BDV with a recombinant vaccinia virus expressing the BDV nucleoprotein and were later infected with BDV to evaluate protection against Borna disease (BD). Relative to animals that were not immuniz...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.3.2541-2546.1999
更新日期:1999-03-01 00:00:00