Abstract:
:Specific cleavage of BK virus (MM) DNA with restriction endonuclease MboI gives rise to 10 fragments. Two techniques were used to determine the location of these fragments on the viral genome with respect to the three known sites for HindIII cleavage. In the first method, reciprocal digestion, individual MboI fragments were digested with HindIII and individual HindIII fragments were digested with MboI. In the second method, single-end 32P-labeled HindIII subfragments were partially digested with MboI, and then the sizes of the radioactive partial products were used to deduce the nearest neighboring fragment. Information from these two methods is more than adequate to map all the MboI enzyme sites. Cleavage of BK virus (MM) DNA with restriction enzyme HaeIII produces 21 fragments. With the aid of the same two methods, these fragments have also been ordered with respect to the known map locations of the HindIII and MboI sites.
journal_name
J Viroljournal_title
Journal of virologyauthors
Yang RC,Wu Rdoi
10.1128/JVI.27.3.700-712.1978subject
Has Abstractpub_date
1978-09-01 00:00:00pages
700-12issue
3eissn
0022-538Xissn
1098-5514journal_volume
27pub_type
杂志文章abstract::Human T-cell leukemia virus type 1 (HTLV-1) encodes a 40-kDa Tax phosphoprotein. Tax is a transcriptional activator which modulates expression of the viral long terminal repeat and transcription of many cellular genes. Because Tax is a critical HTLV-1 factor which mediates viral transformation of T cells during the ge...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.21.11686-11695.2004
更新日期:2004-11-01 00:00:00
abstract::Multiple mutations were found in the human immunodeficiency virus pol gene following treatment of an AIDS patient with antiretroviral drugs. After approximately 2.5 years of monthly alternating therapy with 3'-azido-3'-deoxythymidine (AZT) and 2',3'-dideoxycytidine (ddC), most of the pol sequences amplified from the p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.12.7271-7275.1993
更新日期:1993-12-01 00:00:00
abstract::We investigated the synergism between influenza virus and Streptococcus pneumoniae, particularly the role of deletions in the stalk region of the neuraminidase (NA) of H2N2 and H9N2 avian influenza viruses. Deletions in the NA stalk (ΔNA) had no effect on NA activity or on the adherence of S. pneumoniae to virus-infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05809-11
更新日期:2012-04-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) infection modulates the host cell cycle to create an environment optimal for its viral-DNA replication during the lytic life cycle. We report here that KSHV vIRF4 targets the β-catenin/CBP cofactor and blocks its occupancy on the cyclin D1 promoter, suppressing the G1-S c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01897-15
更新日期:2015-10-21 00:00:00
abstract::The adenovirus E1A transforming region, which encodes immortalization, partial cell transformation, and gene activation functions, expresses two early mRNAs, 13S and 12S. Multiple-T antigen species with different electrophoretic mobilities are formed from each mRNA, presumably by unknown posttranslational modification...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.2.592-599.1986
更新日期:1986-05-01 00:00:00
abstract::Adenovirus type 7 (Ad7) early region 1 mRNA species transcribed in rat cell lines transformed by the HindIII-I . J fragment (the left 7.8% of the viral genome) and in human KB cells infected with Ad7 were mapped on the viral genome, using S1 nuclease gel and diazobenzyloxymethyl paper hybridization techniques. At the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.2.337-352.1980
更新日期:1980-11-01 00:00:00
abstract::The alphavirus Semliki Forest virus (SFV) infects cells through low-pH-induced membrane fusion mediated by the E1 protein, a class II virus membrane fusion protein. During fusion, E1 inserts into target membranes via its hydrophobic fusion loop and refolds to form a stable E1 homotrimer. Mutation of a highly conserved...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00167-06
更新日期:2006-06-01 00:00:00
abstract::Rio Bravo virus (RBV) is a member of the family Flaviviridae, genus Flavivirus. It belongs to a group of viruses in the genus with no known vector. In this report, we analyze the complete genome of the prototype RBV, strain M64. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00331-12
更新日期:2012-04-01 00:00:00
abstract::Human hepatitis B virus (HBV) HBx protein is a multifunctional protein that activates cellular signaling pathways and is thought to be essential for viral infection. Woodchuck HBV mutants that lack HBx are unable to replicate in vivo or are severely impaired. HBV replication in HepG2 cells, a human hepatoblastoma cell...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.14.7713-7719.2003
更新日期:2003-07-01 00:00:00
abstract::The genome of murine cytomegalovirus, extracted from extracellular virions, is a linear double-stranded DNA molecule ca. 240 kilobase pairs long. In our initial cloning of subgenomic fragments of the murine cytomegalovirus genome, we obtained a HindIII clone which contained fused HindIII-terminal fragments. By hybridi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.1.24-28.1984
更新日期:1984-10-01 00:00:00
abstract::Foamy viruses (FVs) are the least known retroviruses commonly found in primates, cats, horses, and cattle. Although FVs are considered apathogenic, simian and feline FVs have been shown to be associated with some transient health abnormalities in animal models. Currently, data regarding the course of infection with bo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02447-12
更新日期:2013-03-01 00:00:00
abstract::Since 2005, a large poliomyelitis outbreak associated with type 2 circulating vaccine-derived poliovirus (cVDPV2) has occurred in northern Nigeria, where immunization coverage with trivalent oral poliovirus vaccine (tOPV) has been low. Phylogenetic analysis of P1/capsid region sequences of isolates from each of the 40...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02954-12
更新日期:2013-05-01 00:00:00
abstract::The human cytidine deaminase Apobec3F (h-A3F), a protein related to the previously recognized antiviral factor Apobec3G (h-A3G), has antiviral activity against human immunodeficiency virus type 1 (HIV-1) that is suppressed by the viral protein Vif. The mechanism of HIV-1 Vif-mediated suppression of h-A3F is not fully ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.15.9579-9587.2005
更新日期:2005-08-01 00:00:00
abstract::We generated a recombinant influenza A virus (Mmut) that produced low levels of matrix (M1) and M2 proteins in infected cells. Mmut virus propagated to significantly lower titers than did wild-type virus in cells infected at low multiplicity. By contrast, virion morphology and incorporation of viral proteins and vRNAs...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.12.7926-7932.2005
更新日期:2005-06-01 00:00:00
abstract::The expression and predicted products of rat c-ros-1 gene, the proto-oncogene of v-ros in UR2 sarcoma virus, were characterized. The c-ros-1 gene was found to be expressed in a tissue-specific manner, and the sizes of its transcripts were heterogeneous: 8.2 kilobases (kb) long in lung and kidney tissues, 6.9 kb in hea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.5.2117-2125.1990
更新日期:1990-05-01 00:00:00
abstract::The bovine papillomavirus type 1 (BPV-1) genome replicates as a multiple-copy plasmid in murine C127 cells transformed to neoplasia by virus infection or by transfection with BPV-1 DNA. It was reported previously that BPV-1 genomes harboring frameshift mutations in the E6 or E7 open reading frame (ORF) replicated in C...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.8.4957-4965.1992
更新日期:1992-08-01 00:00:00
abstract::Under natural conditions and in some experimental models, rabies virus infection of the central nervous system causes relatively mild histopathological changes, without prominent evidence of neuronal death despite its lethality. In this study, the effects of rabies virus infection on the structure of neurons were inve...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01677-07
更新日期:2008-01-01 00:00:00
abstract::Striking morphologic similarities between Machupo, Tacaribe, and lymphocytic choriomeningitis viruses were found by thin-section electron microscopy. It is proposed that these viruses be brought together into a single taxonomic group. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.4.4.535-541.1969
更新日期:1969-10-01 00:00:00
abstract::Mice infected with myopathic coxsackievirus B1 Tucson (CVB1(T)) develop chronic inflammatory myopathy (CIM) consisting of hind limb weakness and inflammation. Amyopathic virus variants are infectious but attenuated for CIM. In this report, viral clones, chimeras, and sequencing were used to identify viral determinants...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.21.11849-11854.2003
更新日期:2003-11-01 00:00:00
abstract::Superinfection of Corynebacterium diphtheriae C7(beta) by heteroimmune phage gamma is productive, whereas superinfection by gamma-bin mutants is for the most part nonproductive. Exclusion of gamma-bin phage occurred after its DNA had penetrated and was partially expressed in the heteroimmune lysogen. All of the infect...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.2.526-532.1980
更新日期:1980-11-01 00:00:00
abstract::The genome of infectious hematopoietic necrosis virus (IHNV), a salmonid novirhabdovirus, has been engineered to modify the gene order and to evaluate the impact on a possible attenuation of the virus in vitro and in vivo By reverse genetics, eight recombinant IHNVs (rIHNVs), termed NxGy according to the respective po...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01024-16
更新日期:2016-11-14 00:00:00
abstract::Relative to wild-type herpes simplex virus type 1 (HSV-1), ICP0-null mutant viruses reactivate inefficiently from explanted, latently infected mouse trigeminal ganglia (TG), indicating that ICP0 is not essential for reactivation but plays a central role in enhancing the efficiency of reactivation. The validity of thes...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.7.3240-3249.2001
更新日期:2001-04-01 00:00:00
abstract::We have previously identified a functionally essential bulged stem-loop in the 3' untranslated region of the positive-stranded RNA genome of mouse hepatitis virus. This 68-nucleotide structure is composed of six stem segments interrupted by five bulges, and its structure, but not its primary sequence, is entirely cons...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.15.6911-6921.2000
更新日期:2000-08-01 00:00:00
abstract::The HIV-1 spike is composed of three protomeric units, each containing a peripheral gp120 and a transmembrane gp41 subunit. Binding to the CD4 and the chemokine receptors triggers them to mediate virus entry into cells by membrane fusion. The spikes also represent the major target for neutralizing antibodies (Abs) aga...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00530-13
更新日期:2013-06-01 00:00:00
abstract::Arginine methylation of human immunodeficiency virus type 1 (HIV-1) Tat protein downregulates its key function in viral-gene transactivation. The fate of methylated Tat is unknown, so it is unclear whether methylated Tat is degraded or persists in the cell for additional functions. Here we show that the arginine methy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00499-09
更新日期:2009-11-01 00:00:00
abstract::Viral infection induces production of type I interferons (IFNs), which stimulate the expression of a variety of antiviral factors to inhibit viral replication. To establish effective infection, viruses need to develop strategies to evade the immune responses. A neurovirulent Sindbis virus strain with neuroinvasive pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01487-16
更新日期:2016-10-28 00:00:00
abstract::Satellite RNAs are the smallest infectious agents whose replication is thought to be completely dependent on their helper virus (HV). Here we report that, when expressed autonomously in the absence of HV, a variant of satellite RNA (satRNA) associated with Cucumber mosaic virus strain Q (Q-satRNA) has a propensity to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00018-12
更新日期:2012-05-01 00:00:00
abstract::Mice were infected with lymphocytic choriomeningitis virus and injected once 24 h later with a monoclonal antibody directed against gamma interferon. In comparison with controls, the increase of numbers of CD8+ T cells and the generation of virus-specific cytotoxic T lymphocytes in spleens and virus clearance from org...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.3.1521-1526.1996
更新日期:1996-03-01 00:00:00
abstract:UNLABELLED:Foreskin is the principal site of heterosexual HIV-1 infection in men. However, little is known about HIV-1-specific immune responses or inflammation in foreskin. To the best of our knowledge, no previous studies have assessed immune responses to candidate HIV-1 vaccines in foreskin. Using the rhesus monkey ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03771-13
更新日期:2014-04-01 00:00:00
abstract::Nucleocapsids were isolated from purified enveloped nucleocapsids of Plodia interpunctella granulosis virus by treatment with Nonidet P-40. When analyzed on sodium dodecyl sulfate-polyacrylamide gels, the nucleocapsids consisted of eight polypeptides. One of these, a major component with a molecular weight of 12,500 (...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.33.2.866-876.1980
更新日期:1980-02-01 00:00:00