Abstract:
:The alphavirus Semliki Forest virus (SFV) infects cells through low-pH-induced membrane fusion mediated by the E1 protein, a class II virus membrane fusion protein. During fusion, E1 inserts into target membranes via its hydrophobic fusion loop and refolds to form a stable E1 homotrimer. Mutation of a highly conserved histidine (the H230A mutation) within a loop adjacent to the fusion loop was previously shown to block SFV fusion and infection, although the mutant E1 protein still inserts into target membranes and forms a homotrimer. Here we report on second-site mutations in E1 that rescue the H230A mutant. These mutations were located in a cluster within the hinge region, at the membrane-interacting tip, and within the groove where the E1 stem is believed to pack. Together the revertants reveal specific and interconnected aspects of the fusion protein refolding reaction.
journal_name
J Viroljournal_title
Journal of virologyauthors
Chanel-Vos C,Kielian Mdoi
10.1128/JVI.00167-06subject
Has Abstractpub_date
2006-06-01 00:00:00pages
6115-22issue
12eissn
0022-538Xissn
1098-5514pii
80/12/6115journal_volume
80pub_type
杂志文章abstract::Rhesus monkey rhadinovirus (RRV), a simian gamma-2 herpesvirus closely related to the Kaposi sarcoma-associated herpesvirus, replicates lytically in cultured rhesus monkey fibroblasts and establishes persistence in B cells. Overlapping cosmid clones were generated that encompass the entire 130-kilobase-pair genome of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.3.1549-1562.2006
更新日期:2006-02-01 00:00:00
abstract::It was demonstrated that Epstein-Barr virus (EBV)-encoded small RNAs (EBERs) were nonessential for B-lymphocyte growth transformation. We revisited this issue by producing a large quantity of EBER-deleted EBV by using an Akata cell system. Although the EBER-deleted virus efficiently infected B lymphocytes, its 50% tra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.7.4298-4307.2005
更新日期:2005-04-01 00:00:00
abstract::The phosphoprotein P of Borna disease virus (BDV) is an essential cofactor of the viral RNA-dependent RNA polymerase. It is preferentially phosphorylated at serine residues 26 and 28 by protein kinase C epsilon (PKCepsilon) and, to a lesser extent, at serine residues 70 and 86 by casein kinase II (CKII). To determine ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02233-06
更新日期:2007-06-01 00:00:00
abstract::The identification of tumor-inducing genes is a driving force for elucidating the molecular mechanisms underlying cancer. Many retroviruses induce tumors by insertion of viral DNA adjacent to cellular oncogenes, resulting in altered expression and/or structure of the encoded proteins. The availability of the mouse gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.3.2056-2062.2003
更新日期:2003-02-01 00:00:00
abstract::Lentiviruses have in their transmembrane glycoprotein (TM) a highly immunogenic structure referred to as the principal immunodominant domain (PID). The PID forms a loop of 5 to 7 amino acids between two conserved cysteines. Previous studies showed that envelope (Env) glycoprotein functions of feline immunodeficiency v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.7.5698-5706.1999
更新日期:1999-07-01 00:00:00
abstract::Genetic studies with immunocompetent mice show the importance of both T cells and gamma interferon (IFN-γ) for survival of a measles virus (MV) challenge; however, the direct role of T cells and IFN-γ within the MV-infected brain has not been addressed. Organotypic brain explants represent a successful ex vivo system ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01496-10
更新日期:2011-04-01 00:00:00
abstract::Four chimeric human immunodeficiency virus type 1 (HIV-1) env genes were constructed which encoded the extracellular domain of either the wild-type or a cleavage-defective HIV-1 envelope glycoprotein (gp160) fused at one of two different positions in env to a C-terminal glycosyl-phosphatidylinositol (GPI) attachment s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.9.5279-5288.1993
更新日期:1993-09-01 00:00:00
abstract::Circulating interferon production, induced by Newcastle disease virus, is about seven times higher in C(57) Black mice than i Balb/c/Gif mice. A Mendelian analysis was carried out and circulating interferon production was measured in reciprocal F(1) hybrids, in the F(2) generation, in progeny of backcrosses of F(1) hy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.3.5.506-512.1969
更新日期:1969-05-01 00:00:00
abstract::Borna disease virus (BDV) is a neurotropic virus with a broad host and geographic range. Lewis rats were immunized against BDV with a recombinant vaccinia virus expressing the BDV nucleoprotein and were later infected with BDV to evaluate protection against Borna disease (BD). Relative to animals that were not immuniz...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.3.2541-2546.1999
更新日期:1999-03-01 00:00:00
abstract::Human cytomegalovirus (HCMV) has emerged as a clinically opportunistic pathogen that targets multiple types of ocular cells and tissues, including the iris region of the uveal tract during anterior uveitis. In this report, we used primary cultures of human iris stroma (HIS) cells derived from human eye donors to inves...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00109-15
更新日期:2015-05-01 00:00:00
abstract::Host cell interactions of human adenovirus serotypes belonging to subgroups B (adenovirus type 3 [Ad3] and Ad7) and C (Ad2 and Ad5) were comparatively analyzed at three levels: (i) binding of virus particles with host cell receptors; (ii) cointernalization of macromolecules with adenovirions; and (iii) adenovirus-indu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.8.3661-3673.1990
更新日期:1990-08-01 00:00:00
abstract::Human cytomegalovirus (HCMV) encodes four putative G protein-coupled receptors, including pUL78, whose rodent orthologues are known to be important for replication and spread in their hosts. To investigate the mechanism by which pUL78 contributes to viral replication and pathogenesis, we generated a derivative of the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05900-11
更新日期:2012-11-01 00:00:00
abstract::Purified nuclei from polyoma-infected mouse (3T3) cells were found to be greatly reduced in their ability to synthesize viral DNA in vitro when compared with a crude system consisting of an unfractionated hypotonic lysate of the infected cells. The synthetic capacity of the nuclei could be fully reconstituted when a h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.1.97-107.1975
更新日期:1975-01-01 00:00:00
abstract::Previously we have described a stepwise, energy-dependent pathway for human immunodeficiency virus type 1 (HIV-1) capsid assembly in a cell-free system. In this pathway, Gag polypeptides utilize the cellular factor HP68 and assemble into immature capsids by way of assembly intermediates that have defined biochemical c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.4.1645-1656.2004
更新日期:2004-02-01 00:00:00
abstract::Natural evolution in primate lentiviral reverse transcriptase (RT) appears to have been constrained by the necessity to maintain function within an asymmetric protein composed of two identical primary amino acid sequences (66 kDa), of which one is cleaved (51 kDa). In this study, a detailed phylogenetic analysis now s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00991-10
更新日期:2010-10-01 00:00:00
abstract::Streptomyces coelicolor is a model system for the study of Streptomyces, a genus of bacteria responsible for the production of many clinically important antibiotics. Here we report the genome sequence of ΦCAM, a new S. coelicolor generalized transducing bacteriophage, isolated from a soil sample originating from Linco...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02681-12
更新日期:2012-12-01 00:00:00
abstract::Varicella zoster virus (VZV) is a neurotropic alphaherpesvirus that causes chickenpox during primary infection and establishes latency in sensory ganglia. Infection of rhesus macaques (RM) with the homologous simian varicella virus (SVV) recapitulates hallmarks of VZV infection. We have shown that an antisense transcr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02369-12
更新日期:2013-02-01 00:00:00
abstract::Cells of the established MvlLu mink line spontaneously released a reverse transcriptase-containing virus after long-term passage in tissue culture. By molecular hybridization, DNA of normal mink cells was found to possess extensive nucleotide sequence homology with a reverse-transcription product of the viral genome, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.1.129-137.1978
更新日期:1978-01-01 00:00:00
abstract::Thirteen point mutations targeting predicted domains conserved in homologous protein kinases were introduced into the UL97 coding region of the human cytomegalovirus. All mutagenized proteins were expressed in cells infected with recombinant vaccinia viruses (rVV). Several mutations drastically reduced ganciclovir (GC...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8898-8901.1999
更新日期:1999-10-01 00:00:00
abstract::Influenza causes significant morbidity in tropical regions; however, unlike in temperate zones, influenza in the tropics is not strongly associated with a given season. We have recently shown that influenza virus transmission in the guinea pig model is most efficient under cold, dry conditions, which are rare in the t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00325-08
更新日期:2008-06-01 00:00:00
abstract::Macaques immunized with uninfected human cells have been shown to be protected from challenge with simian immunodeficiency virus (SIV) propagated in human cells. To identify the potential antigens involved in this protection, macaques were immunized with uninfected human cells, sucrose density gradient-purified cultur...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.5.3117-3124.1995
更新日期:1995-05-01 00:00:00
abstract::A detailed kinetic and quantitative analysis of the early and late biosynthetic events undergone by the human immunodeficiency virus type 1 envelope protein expressed by a recombinant vaccinia virus was performed. Early folding events that occurred in the endoplasmic reticulum included disulfide bond formation (t1/2 a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.4.2047-2055.1991
更新日期:1991-04-01 00:00:00
abstract::Major histocompatibility complex class I (MHC I)-restricted CD8(+) T-cell responses play a pivotal role in anti-human immunodeficiency virus (HIV) immunity and the control of viremia. The rhesus macaque is an important animal model for HIV-related research. Among the MHC I alleles of the rhesus macaque, Mamu-A 02 is p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00350-11
更新日期:2011-07-01 00:00:00
abstract::Structural differences between human leukocyte virus-induced interferon and human fibroblast polyinosinic-polycytidylic acid (rIn-rCn)-induced interferon have been noted in previous studies. This study reports the behavior of human leukocyte and fibroblast interferon, induced by virus and by rIn-rCn, in several lectin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.5.1124-1130.1975
更新日期:1975-11-01 00:00:00
abstract::Chronic hepatitis C virus (HCV) infection is frequently associated with extrahepatic manifestations, including nonmalignant and malignant B-cell lymphoproliferative disorders. It has been reported that specific changes or recurring motifs in the amino acid sequence of the HCV hypervariable region 1 (HVR1) may be assoc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02104-06
更新日期:2007-05-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) Tat protein, which is essential for HIV gene expression and viral replication, is known to mediate pleiotropic effects on various cell functions. For instance, Tat protein is able to regulate the rate of transcription of host cellular genes and to interact with the signa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.13.6846-6854.2004
更新日期:2004-07-01 00:00:00
abstract::A covalently closed circular form of unintegrated viral DNA obtained from NIH 3T3 cells freshly infected with Moloney murine leukemia virus (M-MLV) and a port of the endogenous M-MLV from the BALB/Mo mouse strain have been cloned in bacteriophage lambda. The unintegrated viral DNA was cleaved with restriction endonucl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.1.254-263.1980
更新日期:1980-10-01 00:00:00
abstract::Reverse genetic analyses of negative-strand RNA (NSR) viruses have provided enormous advances in our understanding of animal viruses over the past 20 years, but technical difficulties have hampered application to plant NSR viruses. To develop a reverse genetic approach for analysis of plant NSR viruses, we have engine...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01397-13
更新日期:2013-10-01 00:00:00
abstract::Human T-cell leukemia virus type 1 (HTLV-1) is the etiologic agent of the aggressive and fatal disease adult T-cell leukemia. Previous studies have demonstrated that the HTLV-1-encoded Tax protein inhibits the function of tumor suppressor p53 through a Tax-induced NF-kappaB pathway. Given these attributes, we were int...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00690-08
更新日期:2008-09-01 00:00:00
abstract:UNLABELLED:The positive-sense RNA genome of Sweet potato feathery mottle virus (SPFMV) (genus Potyvirus, family Potyviridae) contains a large open reading frame (ORF) of 3,494 codons translatable as a polyprotein and two embedded shorter ORFs in the -1 frame: PISPO, of 230 codons, and PIPO, of 66 codons, located in the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02360-15
更新日期:2016-01-20 00:00:00