Abstract:
:Human cytomegalovirus (HCMV) encodes four putative G protein-coupled receptors, including pUL78, whose rodent orthologues are known to be important for replication and spread in their hosts. To investigate the mechanism by which pUL78 contributes to viral replication and pathogenesis, we generated a derivative of the TB40/E clinical isolate of HCMV that is unable to express the receptor. Consistent with previous findings using laboratory strains of the virus, the mutant replicated normally in fibroblasts. Although laboratory strains are restricted to growth in fibroblasts, clinical isolates grow in many cell types, including epithelial and endothelial cells, in which the pUL78-deficient TB40/E derivative exhibited a growth defect. Infection with the mutant virus resulted in a significant decrease in viral RNA and protein expression. Although there was no difference in binding of the virus to the cell, we detected a delay in the entry and subsequent delivery of virion DNA and protein to the nuclei of epithelial cells following infection with the UL78 mutant virus. Taken together, our results demonstrate that pUL78 supports infection at a point after binding but before entry in epithelial cells, a cell type important for in vivo viral replication and spread.
journal_name
J Viroljournal_title
Journal of virologyauthors
O'Connor CM,Shenk Tdoi
10.1128/JVI.05900-11subject
Has Abstractpub_date
2012-11-01 00:00:00pages
11425-33issue
21eissn
0022-538Xissn
1098-5514pii
JVI.05900-11journal_volume
86pub_type
杂志文章abstract::The prevalence and importance of microRNAs (miRNAs) in viral infection are increasingly relevant. Eleven miRNAs were previously identified in human cytomegalovirus (HCMV); however, miRNA content in murine CMV (MCMV), which serves as an important in vivo model for CMV infection, has not previously been examined. We hav...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01290-07
更新日期:2007-12-01 00:00:00
abstract::The wild-type strain of vesicular stomatitis virus (VSV) contains in its complete virion (VSV-1, B particles) a minus strand RNA. The principle defective particle of the wild-type strain (VSV-111, T particles) contains a shorter minus strand, homologous to part of the VSV-1 genome. Neither virion contains any detectab...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.11.6.915-925.1973
更新日期:1973-06-01 00:00:00
abstract::Vif(IIIB), which has been a standard model for the viral infectivity factor of human immunodeficiency virus type 1 (HIV-1), binds the cytidine deaminase APOBEC3G (A3G) and induces its degradation, thereby precluding its lethal incorporation into assembling virions. Additionally, Vif(IIIB) less efficiently degrades A3F...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01078-07
更新日期:2008-01-01 00:00:00
abstract::During lytic infection, herpes simplex virus subverts the host cell RNA polymerase II transcription machinery to efficiently express its own genome while repressing the expression of most cellular genes. The mechanism by which RNA polymerase II is directed to the viral delayed-early and late genes is still unresolved....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.988-1001.1994
更新日期:1994-02-01 00:00:00
abstract::Here, we investigated the pre-steady-state deoxynucleoside triphosphate (dNTP) incorporation kinetics of primate foamy virus (PFV) reverse transcriptase (RT) in comparison with those of HIV-1 and MuLV RTs. PFV RT displayed a drastic reduction in primer extension at low dNTP concentrations where HIV-1 RT remains highly...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00088-08
更新日期:2008-08-01 00:00:00
abstract::Unintegrated linear and closed circular DNAs of B- and N-tropic endogenous BALB/c murine leukemia virus (MuLV) were extracted from newly infected mouse cells and cleaved with EcoRI, XhoI, PvuI, HindIII, SalI, XbaI, KpnI, SmaI, and PstI restriction endonucleases. The DNA fragments were separated by electrophoresis and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.3.812-823.1980
更新日期:1980-09-01 00:00:00
abstract:UNLABELLED:The noncovalent interactions that mediate trimerization of the influenza hemagglutinin (HA) are important determinants of its biological activities. Recent studies have demonstrated that mutations in the HA trimer interface affect the thermal and pH sensitivities of HA, suggesting a possible impact on vaccin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02278-13
更新日期:2014-05-01 00:00:00
abstract::The ubiquitous human polyomavirus JC (JCV) is a small double-stranded DNA virus that establishes a persistent infection, and it is often transmitted from parents to children. There are at least 14 subtypes of the virus associated with different human populations. Because of its presumed codivergence with humans, JCV h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00441-06
更新日期:2006-10-01 00:00:00
abstract::A detailed comparison of the gp70 proteins of cloned ecotropic Friend murine leukemia virus (FLV) and dual-tropic Friend mink focus-forming virus (FrMCF) was performed by analyzing the structural and immunological properties of amino- and carboxy-terminal domains of these molecules generated upon controlled trypsiniza...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.2.452-458.1984
更新日期:1984-02-01 00:00:00
abstract::A mutation that disrupts the interaction between the NS2 protein of minute virus of mice and the nuclear export factor Crm1 results in a block to egress of mutant-generated full virions from the nucleus of infected murine cells. These mutants produce wild-type levels of monomer and dimer replicative DNA forms but are ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.7.3257-3266.2002
更新日期:2002-04-01 00:00:00
abstract::Despite being able to use the Bonzo coreceptor as efficiently as CCR5 in transfected cells, pediatric human immunodeficiency virus type 1 isolate P6 was unable to replicate in peripheral blood mononuclear cells (PBMC) lacking the CCR5 receptor. Furthermore, its replication in wild-type PBMC was completely inhibited by...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.3443-3448.1999
更新日期:1999-04-01 00:00:00
abstract::Brome mosaic virus (BMV), a positive-strand RNA virus, encodes two replication proteins: the 2a protein, which contains polymerase-like sequences, and the 1a protein, with N-terminal putative capping and C-terminal helicase-like sequences. These two proteins are part of a multisubunit complex which is necessary for vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7160-7169.1998
更新日期:1998-09-01 00:00:00
abstract::Merkel cell carcinoma (MCC) is the most aggressive skin cancer. Recently, it was demonstrated that human Merkel cell polyomavirus (MCV) is clonally integrated in approximately 80% of MCC tumors. However, direct evidence for whether oncogenic viral proteins are needed for the maintenance of MCC cells is still missing. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02400-09
更新日期:2010-07-01 00:00:00
abstract::To evaluate host range differences between two different strains of feline leukemia virus subgroup B (FeLV-B), we compared the binding and infectivity patterns of retrovirus vectors bearing either FeLV-B-90Z or FeLV-B-GA envelopes. We report here that the ability of these envelopes to utilize different Pit1 orthologs ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.23.12369-12375.2002
更新日期:2002-12-01 00:00:00
abstract::The structural polypeptides of purified Plodia interpunctella granulosis virus were analyzed by three different two-dimensional gel systems. Isoelectric focusing followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis allowed resolution of 53 acidic polypeptides in the enveloped nucleocapsid of the virus...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.1.82-89.1986
更新日期:1986-10-01 00:00:00
abstract:UNLABELLED:A hallmark of Ebola virus (EBOV) infection is the formation of viral inclusions in the cytoplasm of infected cells. These viral inclusions contain the EBOV nucleocapsids and are sites of viral replication and nucleocapsid maturation. Although there is growing evidence that viral inclusions create a protected...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00459-16
更新日期:2016-07-27 00:00:00
abstract:UNLABELLED:Human cytomegalovirus (hCMV) infection is characterized by a vast expansion of resting effector-type virus-specific T cells in the circulation. In mice, interleukin-7 receptor α (IL-7Rα)-expressing cells contain the precursors for long-lived antigen-experienced CD8(+) T cells, but it is unclear if similar me...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02003-14
更新日期:2015-01-01 00:00:00
abstract::The sequence of the human immunodeficiency virus type 2 circle junction was determined. The most common sequence found between the conserved CA and TG dinucleotides at the ends of the integrated provirus was five bases long (GGTAC). This suggests that the integration of human immunodeficiency virus type 2 DNA is accom...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.7.3906-3910.1991
更新日期:1991-07-01 00:00:00
abstract::We have demonstrated that Epstein-Barr virus (EBV) confers enhanced growth capability in soft agarose, tumorigenesis in the SCID mouse, and resistance to apoptosis in the Burkitt's lymphoma cell line Akata. Subsequently, we have shown that EBV-encoded small RNAs (EBERs) are responsible for these phenotypes. We constan...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.3.1561-1564.2001
更新日期:2001-02-01 00:00:00
abstract::Phosphorylation of the murine mammary tumor virus (MuMTV) structural proteins was studied in an MuMTV-infected epithelial cell line derived from a BALB/cf C3H mouse mammary tumor. Immunoprecipitation of 32P-labeled cell extracts with monospecific anti-p27 serum revealed that phosphorylation occurred at the stage of th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.30.1.241-247.1979
更新日期:1979-04-01 00:00:00
abstract::We have used an antisense RNA approach in the analysis of gene function in human cytomegalovirus (HCMV). An astrocytoma cell line (U373-MG) that is permissive for virus replication was permanently transfected with a construct bearing sequence from HCMV UL44 (coding for the major late DNA-binding protein, ppUL44, also ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.4.2047-2057.1995
更新日期:1995-04-01 00:00:00
abstract::The phosphoprotein P of Borna disease virus (BDV) is an essential cofactor of the viral RNA-dependent RNA polymerase. It is preferentially phosphorylated at serine residues 26 and 28 by protein kinase C epsilon (PKCepsilon) and, to a lesser extent, at serine residues 70 and 86 by casein kinase II (CKII). To determine ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02233-06
更新日期:2007-06-01 00:00:00
abstract::Human erythroid progenitor cells are the main target cells of the human parvovirus B19 (B19), and B19 infection induces a transient erythroid aplastic crisis. Several authors have reported that the nonstructural protein 1 (NS-1) encoded by this virus has a cytotoxic effect, but the underlying mechanism of NS-1-induced...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8762-8770.1999
更新日期:1999-10-01 00:00:00
abstract::Lentiviruses are nononcogenic retroviruses that cause persistent infections and slowly progressive diseases. Visna virus, a lentivirus of sheep, persists in cells of the macrophage lineage despite the presence of neutralizing antibodies in the animal. These antibodies are measured by prevention of virus replication in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.59.1.37-44.1986
更新日期:1986-07-01 00:00:00
abstract::The immune response to bovine herpesvirus 1 (BHV-1) infection can protect cattle from subsequent challenge with the virus. This protection involves a variety of defensive strategies, and the activation of most of these defenses requires the recognition of viral proteins by the cellular immune system. To identify some ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.10.5114-5122.1990
更新日期:1990-10-01 00:00:00
abstract::The amphotropic murine leukemia virus (MuLV) can infect cells from a number of mammals, including humans, via its specific receptor. Basic knowledge of amphotropic MuLV receptor expression is likely to be useful in the development and improvement of gene therapy protocols based on amphotropic-pseudotyped vectors. To i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.6.4564-4570.1997
更新日期:1997-06-01 00:00:00
abstract::Characterization of immune responses induced by live attenuated simian immunodeficiency virus (SIV) strains may yield clues to the nature of protective immunity induced by this vaccine approach. We investigated the ability of CD8(+) T lymphocytes from rhesus macaques immunized with the live, attenuated SIV strain SIVm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.8.6315-6324.1998
更新日期:1998-08-01 00:00:00
abstract::The genome-length, dicistronic mRNA of the double-stranded RNA fungal virus Helminthosporium victoriae virus 190S (genus Victorivirus, family Totiviridae) contains two long open reading frames (ORFs) that overlap in the tetranucleotide AUGA. Translation of the downstream ORF, which encodes the RNA-dependent RNA polyme...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00364-11
更新日期:2011-07-01 00:00:00
abstract::Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3 (APOBEC3) proteins are encapsidated by assembling HIV-1 virions and edit viral cDNA in the next round of infection. Using alpha interferon (IFN-α)-treated monocyte-derived macrophages, we show that infrequent editing of HIV-1 reverse transcripts can als...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00775-11
更新日期:2011-12-01 00:00:00
abstract::In humans, homologous to the E6-AP carboxyl terminus (HECT) and regulator of chromosome condensation 1 (RCC1)-like domain-containing protein 5 (HERC5) is an interferon-induced protein that inhibits replication of evolutionarily diverse viruses, including human immunodeficiency virus type 1 (HIV-1). To better understan...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00528-18
更新日期:2018-06-13 00:00:00