Abstract:
:Brome mosaic virus (BMV), a positive-strand RNA virus, encodes two replication proteins: the 2a protein, which contains polymerase-like sequences, and the 1a protein, with N-terminal putative capping and C-terminal helicase-like sequences. These two proteins are part of a multisubunit complex which is necessary for viral RNA replication. We have previously shown that the yeast two-hybrid assay consistently duplicated results obtained from in vivo RNA replication assays and biochemical assays of protein-protein interaction, thus permitting the identification of additional interacting domains. We now map an interaction found to take place between two 1a proteins. Using previously characterized 1a mutants, a perfect correlation was found between the in vivo phenotypes of these mutants and their abilities to interact with wild-type 1a (wt1a) and each other. Western blot analysis revealed that the stabilities of many of the noninteracting mutant proteins were similar to that of wt1a. Deletion analysis of 1a revealed that the N-terminal 515 residues of the 1a protein are required and sufficient for 1a-1a interaction. This intermolecular interaction between the putative capping domain and itself was detected in another tripartite RNA virus, cucumber mosaic virus (CMV), suggesting that the 1a-1a interaction is a feature necessary for the replication of tripartite RNA viruses. The boundaries for various activities are placed in the context of the predicted secondary structures of several 1a-like proteins of members of the alphavirus-like superfamily. Additionally, we found a novel interaction between the putative capping and helicase-like portions of the BMV and CMV 1a proteins. Our cumulative data suggest a working model for the assembly of the BMV RNA replicase.
journal_name
J Viroljournal_title
Journal of virologyauthors
O'Reilly EK,Wang Z,French R,Kao CCdoi
10.1128/JVI.72.9.7160-7169.1998subject
Has Abstractpub_date
1998-09-01 00:00:00pages
7160-9issue
9eissn
0022-538Xissn
1098-5514journal_volume
72pub_type
杂志文章abstract::By superinfection of human immunodeficiency virus type 2 (HIV-2) strain HIV-2ben-infected macaques with simian immunodeficiency virus (SIV) strain SIVmac, we investigated the mutual influences of an apathogenic and a pathogenic virus in vivo. Four rhesus and two cynomolgus monkeys were infected with HIV-2ben in 1988 a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.3.1564-1574.1995
更新日期:1995-03-01 00:00:00
abstract::Monoclonal antibodies (MAbs) were raised against human immunodeficiency virus type 1 gp120. One MAb, P4/D10, was found to mediate highly efficient antibody-dependent cellular cytotoxicity and virus neutralization. The reactivity was located to a major neutralizing region (amino acids 304 to 323) on gp120. Five other M...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.2.936-940.1990
更新日期:1990-02-01 00:00:00
abstract::An adenovirus vector encoding murine Fas ligand (mFasL) under an inducible control was derived. In vivo ectopic expression of mFasL in murine livers induced an inflammatory cellular infiltration. Furthermore, ectopic expression of mFasL by myocytes did not allow prolonged vector-mediated transgene expression. Thus, ec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.2483-2490.1998
更新日期:1998-03-01 00:00:00
abstract::ev 2 is one of seven recently described genetic loci of chickens which contain structural genes for endogenous virus. ev 2 is present exclusively in line 72 chickens, an inbred strain of white Leghorns which is homozygous for the capacity to produce Rous-associated virus type 0 (RAV-0), a subgroup E virus. This phenot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.33.1.250-255.1980
更新日期:1980-01-01 00:00:00
abstract::Molecularly cloned proviral DNA of avian oncogenic retrovirus CMII was isolated by screening a genomic library of a CMII-transformed quail cell line with a myc-specific probe. On a 10.4-kilobase EcoRI fragment, the cloned DNA contained 4.4 kilobases of CMII proviral sequences extending from the 5' long terminal repeat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.2.576-585.1985
更新日期:1985-05-01 00:00:00
abstract::Klebsiella pneumoniae MirM7 is a wild-type strain which grows as cocci at pH 7 and above and as rods at pH 6.5 and below. Cultures of this strain and an auxotrophic derivative, MirM7b, have been found to undergo spontaneous lysis after purification from possible contaminating viruses. Lysates always contained two phag...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.3.772-785.1978
更新日期:1978-12-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) lytic infection increases the expression of viral and human interleukin-6 (vIL-6 and hIL-6, respectively), an important factor for cell growth and pathogenesis. Here, we report genome-wide analysis of viral RNA targets of KSHV ORF57 by a novel UV-cross-linking and immunop...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02144-10
更新日期:2011-03-01 00:00:00
abstract::Early in infection by bacteriophage T4, before replication has commenced, one can detect the presence of newly synthesized DNA which cosediments with parental phage DNA on sucrose gradients. As shown earlier (R. E. Murray and C. K. Mathews, 1969), some of this represents covalent attachment of new material to parental...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.4.847-857.1974
更新日期:1974-04-01 00:00:00
abstract::Papilloma virus DNA from a laryngeal papilloma was cloned in phage lambda L 47 and characterized after cleavage with different restriction enzymes. Hybridization with the DNAs of human papilloma virus types 1, 2, 3, 4, 5, and 8 showed no homology under stringent hybridization conditions. Human papilloma virus type 6 D...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.1.393-400.1982
更新日期:1982-10-01 00:00:00
abstract::The tsB5 mutant of herpes simplex virus type 1 (HSV-1) strain HFEM was shown previously to be temperature sensitive for accumulation of the mature form of glycoprotein gB, for production or activity of a factor required in virus-induced cell fusion, and for production of virions with normal levels of infectivity. In a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.1.114-128.1980
更新日期:1980-07-01 00:00:00
abstract::Rodent fibroblasts infected with the ts371 Kirsten murine sarcoma virus (KiMuSV) are temperature sensitive for the maintenance of transformation because of the production of an abnormal p21 protein. We cloned the ts371 KiMuSV provirus from the genome of a conditionally transformed nonproducer cell line, ts371 KiMuSV N...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.2.782-786.1986
更新日期:1986-11-01 00:00:00
abstract::TMC114, a newly designed human immunodeficiency virus type 1 (HIV-1) protease inhibitor, is extremely potent against both wild-type (wt) and multidrug-resistant (MDR) viruses in vitro as well as in vivo. Although chemically similar to amprenavir (APV), the potency of TMC114 is substantially greater. To examine the bas...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.21.12012-12021.2004
更新日期:2004-11-01 00:00:00
abstract::Viral hemorrhagic septicemia virus (VHSV) infections cause high losses in cultured rainbow trout in Europe. Attempts to produce a recombinant vaccine based on the transmembrane glycoprotein (G protein) have indicated that proper folding is important for the antigenicity and immunogenicity of the protein. The present s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.12.10189-10196.1998
更新日期:1998-12-01 00:00:00
abstract::Human herpesvirus 8 (HHV8) downregulates major histocompatibility complex (MHC) class I complexes from the plasma membrane via two of its genes, K3 and K5. The N termini of K3 and K5 contain a plant homeodomain (PHD) predicted to be structurally similar to RING domains found in E3 ubiquitin ligases. In view of the imp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.11.5522-5531.2002
更新日期:2002-06-01 00:00:00
abstract::There are no known RNA viruses that infect Archaea. Filling this gap in our knowledge of viruses will enhance our understanding of the relationships between RNA viruses from the three domains of cellular life and, in particular, could shed light on the origin of the enormous diversity of RNA viruses infecting eukaryot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.07196-11
更新日期:2012-05-01 00:00:00
abstract::The envelope glycoproteins of the human immunodeficiency virus (HIV) type 1 are synthesized as a precursor molecule, gp160, which is cleaved to generate the two mature envelope glycoproteins, gp120 and gp41. The cleavage reaction, which is mediated by a host protease, occurs at a sequence highly conserved in retrovira...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.11.4670-4675.1989
更新日期:1989-11-01 00:00:00
abstract::Our study was designed to investigate the nature of an antigen-specific suppressor factor generated by antigen-stimulated herpes simplex virus (HSV)-immune splenocytes. Factor SF-200, a 90,000- to 100,000-dalton fraction obtained after Sephacryl gel filtration, suppressed the generation of HSV-specific cytotoxic T-lym...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.2.331-338.1986
更新日期:1986-05-01 00:00:00
abstract::RNA-dependent protein kinase PKR is an important regulator of gene expression in interferon (IFN)-treated and virus-infected cells. The 50-kb gene encoding human PKR kinase (pkr) is inducible by IFN. Transfection analyses, using chloramphenicol acetyltransferase (CAT) as the reporter in constructs possessing various 5...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.12.9934-9939.1998
更新日期:1998-12-01 00:00:00
abstract::Carcinogenic progression of a human papillomavirus (HPV)-infected cell is often associated with integration of the viral genome in a manner which results in the loss of expression of the viral regulatory protein E2. One function of E2 is the regulation of expression of the viral oncogenes, E6 and E7. Introduction of t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.12.7791-7799.1995
更新日期:1995-12-01 00:00:00
abstract::The human T-cell leukemia virus type I (HTLV-I) envelope protein is synthesized as a gp61 precursor product cleaved into two mature proteins, a gp45 exterior protein and a gp20 anchoring the envelope at the cell membrane. Using N-glycosylation inhibitors and site-directed mutagenesis of the potential glycosylation sit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.2.906-913.1992
更新日期:1992-02-01 00:00:00
abstract::Polyacrylamide gel electrophoresis and tryptic peptide fingerprint analysis of the proteins made in a cell-free system derived from L-cells and immunoprecipitated with simian virus 40 (SV40) anti-T serum demonstrated that both SV40 large-T and small-T antigens are synthesized in vitro in response to mRNA isolated from...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.1.154-170.1978
更新日期:1978-10-01 00:00:00
abstract::Hepatitis C virus (HCV) is a major causative agent of posttransfusion non-A, non-B hepatitis, which often develops into malignant chronic diseases, including liver cirrhosis and hepatocellular carcinoma. We have cloned from human carriers overlapping cDNAs (9,416 bp) covering the entire coding region of the HCV genome...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.3.1105-1113.1991
更新日期:1991-03-01 00:00:00
abstract::Particular interest in human T lymphocyte lymphoma/leukemia virus (HTLV) derives from the close association of HTLV with several types of human mature T lymphocyte malignancies and the strong possibility that HTLV is the causative agent of this group of leukemias and lymphomas. This is the first report to show that HT...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.53.2.440-446.1985
更新日期:1985-02-01 00:00:00
abstract::Transgenic mice expressing the simian virus 40 large T antigen (TAg) in enterocytes develop intestinal hyperplasia that progresses to dysplasia with age. This induction requires TAg action on the retinoblastoma (Rb) family of tumor suppressors and is independent of the p53 pathway. In cell culture systems, the inactiv...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00922-07
更新日期:2007-09-01 00:00:00
abstract::Strains of Bacillus that harbor defective phage PBSX were found to be insensitive to SP-10(C), although the phage adsorbed to these insensitive strains. Strains that did not carry the phage were sensitive to SP-10(C). B. subtilis 168 ind(-), which can be tranduced by SP-10(C) but is nonpermissive for the phage, was re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:
更新日期:1968-08-01 00:00:00
abstract::Reovirus attachment protein σ1 engages glycan receptors and junctional adhesion molecule-A (JAM-A) and is thought to undergo a conformational change during the proteolytic disassembly of virions to infectious subvirion particles (ISVPs) that accompanies cell entry. The σ1 protein is also the primary target of neutrali...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01621-16
更新日期:2017-01-31 00:00:00
abstract::A streptomycin-resistant Escherichia coli mutant has been isolated that is temperature sensitive for Qbeta phage, but not for the group I RNA phages f2, MS2, and R17. The growth of Qbeta in the mutant at the nonpermissive temperature (42 degrees C) results in the release of a near-normal burst of noninfectious particl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.1.1-6.1977
更新日期:1977-01-01 00:00:00
abstract::The sensitivity of highly purified human fibroblast interferon and partially purified human leukocyte interferon to several proteolytic and glycolytic enzymes was determined with respect to antiviral activity, isoelectric point, molecular weight, and thermal stability. Leucine aminopeptidase altered the distribution o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.2.390-399.1980
更新日期:1980-08-01 00:00:00
abstract::Comparative surface feature analyses of the VP1 sequences of hepatitis A virus (HAV) and poliovirus type 1 allowed an alignment of the two sequences and an identification of probable HAV neutralization antigenic sites. A synthetic peptide containing the HAV-specific amino acid sequence of one of these sites induced an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.55.3.836-839.1985
更新日期:1985-09-01 00:00:00
abstract::Both the RNase H domain of Moloney murine leukemia virus (Mo-MLV) reverse transcriptase (RT) and Escherichia coli RNase H possess a positively charged alpha-helix (C helix) and a loop that are not present in the RNase H domains of human immunodeficiency virus (HIV) RT or avian sarcoma virus RT. Although a mutant Mo-ML...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.16.8360-8373.2002
更新日期:2002-08-01 00:00:00