Abstract:
:Human herpesvirus 8 (HHV8) downregulates major histocompatibility complex (MHC) class I complexes from the plasma membrane via two of its genes, K3 and K5. The N termini of K3 and K5 contain a plant homeodomain (PHD) predicted to be structurally similar to RING domains found in E3 ubiquitin ligases. In view of the importance of the ubiquitin-proteasome system in sorting within the endocytic pathway, we analyzed its role in downregulation of MHC class I complexes in cells expressing K3. Proteasome inhibitors as well as cysteine and aspartyl protease inhibitors stabilize MHC class I complexes in cells expressing K3. However, proteasome inhibitors differentially affect sorting of MHC class I complexes within the endocytic pathway and prevent their delivery to a dense endosomal compartment. In this compartment, the cytoplasmic tail of MHC class I complexes is cleaved by cysteine proteases. The complex is then cleaved within the plane of the membrane by an aspartyl protease, resulting in a soluble MHC class I fragment composed of the lumenal domain of the heavy chain, beta(2)-microglobulin (beta(2)m), and peptide. We conclude that K3 not only directs internalization, but also targets MHC class I complexes to a dense endocytic compartment on the way to lysosomes in a ubiquitin-proteasome-dependent manner.
journal_name
J Viroljournal_title
Journal of virologyauthors
Lorenzo ME,Jung JU,Ploegh HLdoi
10.1128/jvi.76.11.5522-5531.2002subject
Has Abstractpub_date
2002-06-01 00:00:00pages
5522-31issue
11eissn
0022-538Xissn
1098-5514journal_volume
76pub_type
杂志文章abstract::A detailed kinetic and quantitative analysis of the early and late biosynthetic events undergone by the human immunodeficiency virus type 1 envelope protein expressed by a recombinant vaccinia virus was performed. Early folding events that occurred in the endoplasmic reticulum included disulfide bond formation (t1/2 a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.4.2047-2055.1991
更新日期:1991-04-01 00:00:00
abstract::Regulation of immediate-early gene expression in human cytomegalovirus is subject to complex controls. The major immediate-early (mIE) gene is regulated by both positive and negative regulatory signals, including autoregulation mediated by a cis-repressive sequence. A second immediate-early gene, the US3 gene, is tran...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.9.5362-5367.1995
更新日期:1995-09-01 00:00:00
abstract::Hepatitis C virus (HCV) infection is a prominent risk factor for the development of hepatocellular carcinoma (HCC). Similar to most solid tumors, HCCs are believed to contain poorly differentiated cancer stem cell-like cells (CSCs) that initiate tumorigenesis and confer resistance to chemotherapy. In these studies, we...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05920-11
更新日期:2011-12-01 00:00:00
abstract::ICP0, one of the five immediate-early (IE) gene products of herpes simplex virus (HSV), is a potent activator of transcription. To assess the biological activities of ICP0 and to explore its mechanisms of action, two helper-independent recombinant adenoviruses were constructed. In each recombinant, the E1 region was s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.12.4544-4553.1988
更新日期:1988-12-01 00:00:00
abstract::The effect of bacteriophage SPO1 infection of Bacillus subtilis and a deoxyribonucleic acid (DNA) polymerase-deficient (pol(-)) mutant of this microorganism on the synthesis of DNA has been examined. Soon after infection, the incorporation of deoxyribonucleoside triphosphates into acid-insoluble material by cell lysat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.2.263-272.1972
更新日期:1972-02-01 00:00:00
abstract::Using in situ hybridization, we demonstrated that latent herpes simplex virus type 1 (HSV-1) gene expression is prevalent in human adult nodose ganglia. This suggests that infection of gastrointestinal sensory nerves, probably through swallowed virus-laden oral secretions, occurs commonly and that HSV-1 reactivating f...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.5.4103-4106.1997
更新日期:1997-05-01 00:00:00
abstract::Cytotoxic T lymphocytes (CTL) play an important role in recovery from a number of viral infections. They are also implicated in virus-induced immunopathology as best demonstrated in lymphocytic choriomeningitis virus (LCMV) infection of adult immunocompetent mice. In the present study, the structure of the T-cell rece...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.12.5919-5926.1990
更新日期:1990-12-01 00:00:00
abstract::The Epstein-Barr virus BZLF1 gene product ZEBRA is a DNA-binding protein that is partially homologous to c-Fos, binds specifically to AP-1 sites, and can induce the lytic cycle in latently infected B lymphocytes. Induction of the viral lytic cycle can also be achieved by treatment with the phorbol ester 12-O-tetrade-c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.9.4549-4552.1990
更新日期:1990-09-01 00:00:00
abstract::Following subcutaneous inoculation of newborn Wistar-Furth rats with human adenovirus type 9 (Ad9), 16 of 16 female and 0 of 11 male rats developed mammary tumors. Tumor-positive animals usually developed tumors in multiple glands. Histopathological analyses indicated that three general categories of tumor could be id...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.6.3192-3202.1991
更新日期:1991-06-01 00:00:00
abstract::We introduced various mutations into the activation and RNA binding domains of human immunodeficiency virus type 1 (HIV-1) Tat in order to develop a novel and potent transdominant Tat protein and to characterize its mechanism of action. The different mutant Tat proteins were characterized for their abilities to activa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.8055-8063.1996
更新日期:1996-11-01 00:00:00
abstract::The interaction of papillomavirus E2 proteins with cellular Brd4 protein is important for transcriptional regulation of viral genes and partitioning of viral genomes. Bovine papillomavirus type 1 (BPV-1) E2 binds cellular chromatin in complex with Brd4 in both mitotic and interphase cells. To identify specific sites o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02275-08
更新日期:2009-03-01 00:00:00
abstract::The restrictive properties of tripartite motif-containing 5 alpha (TRIM5α) from small ruminant species have not been explored. Here, we identify highly similar TRIM5α sequences in sheep and goats. Cells transduced with ovine TRIM5α effectively restricted the lentivirus visna/maedi virus DNA synthesis. Proteasome inhib...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00440-12
更新日期:2012-09-01 00:00:00
abstract::Some retroviruses contain monoubiquitinated Gag and do not bud efficiently from cells treated with proteasome inhibitors, suggesting an interaction between the ubiquitin-proteasome system and retrovirus assembly. We examined equine infectious anemia virus (EIAV) particles and found that approximately 2% of the p9(Gag)...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.6.3038-3044.2002
更新日期:2002-03-01 00:00:00
abstract:UNLABELLED:Cell culture (cc)-derived hepatitis B virus (HBV) can infect differentiated HepaRG cells, but efficient infection requires addition of polyethylene glycol (PEG) during inoculation. Identification of sodium taurocholate cotransporting polypeptide (NTCP) as an HBV receptor enabled ccHBV infection of NTCP recon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01153-16
更新日期:2016-08-26 00:00:00
abstract::The human immunodeficiency virus type 1 Vpu protein is a 16-kDa phosphoprotein which enhances the efficiency of virion production and induces rapid degradation of CD4, the cellular receptor for human immunodeficiency virus. The topology of membrane-inserted Vpu was investigated by using in vitro-synthesized Vpu cotran...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.8.5056-5061.1993
更新日期:1993-08-01 00:00:00
abstract::Nipah virus is an emerging, highly pathogenic, zoonotic virus of the Paramyxoviridae family. Human transmission occurs by close contact with infected animals, the consumption of contaminated food, or, occasionally, via other infected individuals. Currently, we lack therapeutic or prophylactic treatments for Nipah viru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01461-17
更新日期:2017-11-14 00:00:00
abstract::Bats are the second largest group of mammals on earth and act as reservoirs of many emerging viruses. In this study, a novel bat adenovirus (AdV) (BtAdV-TJM) was isolated from bat fecal samples by using a bat primary kidney cell line. Infection studies indicated that most animal and human cell lines are susceptible to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02497-09
更新日期:2010-04-01 00:00:00
abstract::A novel circular DNA virus sequence is reported from grapevine. The corresponding genomic organization, coding potential, and conserved origin of replication are similar to those of members of the family Geminiviridae, but the genome of 3,206 nucleotides is 4% larger than the largest reported geminiviral genome and sh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00943-12
更新日期:2012-07-01 00:00:00
abstract::Respiratory syncytial virus (RSV) is a major cause of severe lower respiratory tract disease in infants and the elderly, but no safe and effective RSV vaccine is yet available. For reasons that are not well understood, RSV is only weakly immunogenic, and reinfection occurs throughout life. This has complicated the sea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.3.1130-1139.2006
更新日期:2006-02-01 00:00:00
abstract::Nine pre-early polypeptides have been detected after infection with bacteriophage T5 and 10 pre-early polypeptides have been detected after infection with bacteriophage BF23. Only about one-half of the coding capacity of the redundant ends of the phage DNA, which code for pre-early proteins, is needed for these 9 to 1...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.22.2.480-488.1977
更新日期:1977-05-01 00:00:00
abstract::Neurotropic herpesviruses depend on long-distance axon transport for the initial establishment of latency in peripheral ganglia (retrograde transport) and for viral spread in axons to exposed body surfaces following reactivation (anterograde transport). Images of neurons infected with herpes simplex virus type 1 (HSV-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01296-10
更新日期:2010-12-01 00:00:00
abstract::Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is known to take an endosomal pathway for cell entry; however, it is thought to enter directly from the cell surface when a receptor-bound virion spike (S) protein is affected by trypsin, which induces cleavage of the S protein and activates its fusion po...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01412-08
更新日期:2008-12-01 00:00:00
abstract::Vaccinia extracellular enveloped virus (EEV) is critical for cell-to-cell and long-range virus spread both in vitro and in vivo. The B5R gene encodes an EEV-specific type I membrane protein that is essential for efficient EEV formation. The majority of the B5R ectodomain consists of four domains with homology to short...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.10.6260-6271.2005
更新日期:2005-05-01 00:00:00
abstract::A recombinant vesicular stomatitis virus (VSV-PeGFP-M-MmRFP) encoding enhanced green fluorescent protein fused in frame with P (PeGFP) in place of P and a fusion matrix protein (monomeric red fluorescent protein fused in frame at the carboxy terminus of M [MmRFP]) at the G-L gene junction, in addition to wild-type (wt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01668-08
更新日期:2009-03-01 00:00:00
abstract::The major immediate-early (MIE) gene of human cytomegalovirus (HCMV) expresses IE86, IE72, IE55, and IE18 mRNA by differential splicing. Reverse transcription-PCR with IE72-specific primers generated an 0.65-kb cDNA from HCMV-infected fibroblast RNA, which does not correspond to any known MIE cDNA. Nucleotide sequenci...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.7.3158-3167.2002
更新日期:2002-04-01 00:00:00
abstract::West Nile virus (WNV) replicates in the skin; however, cell targets in the skin have not been identified. In the current studies, WNV infected the epidermis and adnexal glands of mouse skin, and the epidermal cells were identified as keratinocytes by double labeling for WNV antigen and keratin 10. Inoculation of mice ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02692-10
更新日期:2011-05-01 00:00:00
abstract:UNLABELLED:Human cytomegalovirus (hCMV) infection is characterized by a vast expansion of resting effector-type virus-specific T cells in the circulation. In mice, interleukin-7 receptor α (IL-7Rα)-expressing cells contain the precursors for long-lived antigen-experienced CD8(+) T cells, but it is unclear if similar me...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02003-14
更新日期:2015-01-01 00:00:00
abstract::Infection of primary or secondary cultures of Chinese hamster embryo cells with simian virus 40 at a multiplicity of 20 to 50 induced synthesis of the virus-specific intranuclear T antigen in 80 to 90% of the cells within 48 to 72 hr. In the infected cultures, 30 to 50% more cells were recruited into deoxyribonucleic ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.6.6.738-749.1970
更新日期:1970-12-01 00:00:00
abstract::Immunological comparisons were made of baculovirus structural proteins by using a modification of the radioimmunological techniques described by Renart et al. (Proc. Natl. Acad. Sci. U.S.A. 76: 3116-3120, 1979) and Towbin et al. (Proc. Natl. Acad. Sci. U.S.A. 76: 4350-4354, 1979). Viral proteins were electrophoresed i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.1.125-137.1981
更新日期:1981-07-01 00:00:00
abstract::We previously showed that the cellular proteins ZEB1 and ZEB2/SIP1 both play key roles in regulating the latent-lytic switch of Epstein-Barr Virus (EBV) by repressing BZLF1 gene expression. We investigated here the effects of cellular microRNA (miRNA) 200 (miR200) family members on the EBV infection status of cells. W...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00923-10
更新日期:2010-10-01 00:00:00