Abstract:
:Nipah virus is an emerging, highly pathogenic, zoonotic virus of the Paramyxoviridae family. Human transmission occurs by close contact with infected animals, the consumption of contaminated food, or, occasionally, via other infected individuals. Currently, we lack therapeutic or prophylactic treatments for Nipah virus. To develop these agents we must now improve our understanding of the host-virus interactions that underpin a productive infection. This aim led us to perform the present work, in which we identified 101 human-Nipah virus protein-protein interactions (PPIs), most of which (88) are novel. This data set provides a comprehensive view of the host complexes that are manipulated by viral proteins. Host targets include the PRP19 complex and the microRNA (miRNA) processing machinery. Furthermore, we explored the biologic consequences of the interaction with the PRP19 complex and found that the Nipah virus W protein is capable of altering p53 control and gene expression. We anticipate that these data will help in guiding the development of novel interventional strategies to counter this emerging viral threat.IMPORTANCE Nipah virus is a recently discovered virus that infects a wide range of mammals, including humans. Since its discovery there have been yearly outbreaks, and in some of them the mortality rate has reached 100% of the confirmed cases. However, the study of Nipah virus has been largely neglected, and currently we lack treatments for this infection. To develop these agents we must now improve our understanding of the host-virus interactions that underpin a productive infection. In the present work, we identified 101 human-Nipah virus protein-protein interactions using an affinity purification approach coupled with mass spectrometry. Additionally, we explored the cellular consequences of some of these interactions. Globally, this data set offers a comprehensive and detailed view of the host machinery's contribution to the Nipah virus's life cycle. Furthermore, our data present a large number of putative drug targets that could be exploited for the treatment of this infection.
journal_name
J Viroljournal_title
Journal of virologyauthors
Martinez-Gil L,Vera-Velasco NM,Mingarro Idoi
10.1128/JVI.01461-17subject
Has Abstractpub_date
2017-11-14 00:00:00issue
23eissn
0022-538Xissn
1098-5514pii
JVI.01461-17journal_volume
91pub_type
杂志文章abstract::Gene mapping experiments show that Fis-1, a preferred integration region for Friend murine leukemia virus in hematopoietic neoplasms, is extremely closely linked to Int-2, a preferred integration region for mouse mammary tumor virus in mammary carcinomas. Studies at the RNA and DNA level prove that these loci are dist...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.3.1156-1158.1986
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abstract:UNLABELLED:Semen enhances HIV infection in vitro, but how long it retains this activity has not been carefully examined. Immediately postejaculation, semen exists as a semisolid coagulum, which then converts to a more liquid form in a process termed liquefaction. We demonstrate that early during liquefaction, semen exh...
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.1.307-311.1985
更新日期:1985-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.8.4848-4858.2005
更新日期:2005-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2009-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jvi.74.2.1033-1037.2000
更新日期:2000-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00841-20
更新日期:2020-07-30 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.15.7473-7484.2002
更新日期:2002-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.7.4292-4298.1995
更新日期:1995-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.6.3943-3954.1994
更新日期:1994-06-01 00:00:00
abstract::We have developed a system for producing murine leukemia virus (MLV) pseudotyped with human hepatitis B virus (HBV) large (L) and small (S) surface antigens (HBsAg) for targeting primary human hepatocytes. Using the MLV(HBV) pseudotype virus containing a beta-galactosidase reporter gene, we demonstrated that this pseu...
journal_title:Journal of virology
pub_type: 杂志文章,收录出版
doi:10.1128/jvi.76.2.912-917.2002
更新日期:2002-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.03176-14
更新日期:2015-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1971-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2019-11-26 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2003-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.8.2480-2488.1987
更新日期:1987-08-01 00:00:00
abstract::Cytomegalovirus (CMV) is a betaherpesvirus that latently infects most adult humans worldwide and is a major cause of morbidity and mortality in immunocompromised hosts. Latent human CMV (HCMV) is believed to reside in precursors of myeloid-lineage leukocytes and monocytes, which give rise to macrophages and dendritic ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01138-17
更新日期:2017-12-14 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.3.1274-1283.2001
更新日期:2001-02-01 00:00:00
abstract::The DNA synthesized in the nonpermissive host by the noncomplementing mutants am36 and am42 of bacteriophage phi W-14 contains about half the wild-type level of alpha-putrescinylthymine (putThy) and a correspondingly greater level of thymine. The mechanisms whereby thymine nucleotides are excluded from replicating DNA...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.47.3.399-405.1983
更新日期:1983-09-01 00:00:00
abstract::Bovine herpesvirus 1 (BHV-1) is an important pathogen of cattle, and infection is usually initiated via the ocular or nasal cavity. Following acute infection, the primary site for BHV-1 latency is the sensory neuron. Reactivation from latency occurs sporadically, resulting in virus shedding and transmission to uninfec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.13.6771-6779.2002
更新日期:2002-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00490-19
更新日期:2019-08-28 00:00:00
abstract::Bluetongue virus (BTV), a member of the Reoviridae family, is an insect-borne animal pathogen. Virus release from infected cells is predominantly by cell lysis, but some BTV particles are also released from the plasma membrane. The nonstructural protein NS3 has been implicated in this process. Using alternate initiato...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02352-10
更新日期:2011-05-01 00:00:00
abstract::Human group C adenoviruses cause an acute infection in respiratory epithelia and establish a long-term or persistent infection, possibly in lymphocytes. The mechanism by which this persistence is maintained is unknown; however, it would require that persistently infected lymphocytes not be deleted. The adenovirus geno...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.19.9716-9723.2002
更新日期:2002-10-01 00:00:00