Abstract:
:Human group C adenoviruses cause an acute infection in respiratory epithelia and establish a long-term or persistent infection, possibly in lymphocytes. The mechanism by which this persistence is maintained is unknown; however, it would require that persistently infected lymphocytes not be deleted. The adenovirus genome encodes proteins that prevent the immune system from eliminating the virus-infected cell, including the E3 receptor internalization and degradation (RID) complex. The RID complex prevents death of infected cells by blocking apoptosis initiated through death domain-containing receptors of the tumor necrosis factor receptor (TNFR) superfamily, including TNFR1 (L. R. Gooding, T. S. Ranheim, A. E. Tollefson, L. Aquino, P. Duerksen-Hughes, T. M. Horton, and W. S. Wold, J. Virol. 65:4114-4123, 1991), TNF-related apoptosis-inducing ligand receptors (TRAIL-R1 and -R2) (C. A. Benedict, P. S. Norris, T. I. Prigozy, J. L. Bodmer, J. A. Mahr, C. T. Garnett, F. Martinon, J. Tschopp, L. R. Gooding, and C. F. Ware, J. Biol. Chem. 276:3270-3278, 2001; A. E. Tollefson, K. Toth, K. Doronin, M. Kuppuswamy, O. A. Doronina, D. L. Lichtenstein, T. W. Hermiston, C. A. Smith, and W. S. Wold, J. Virol. 75:8875-8887, 2001), and Fas (J. Shisler, C. Yang, B. Walter, C. F. Ware, and L. R. Gooding, J. Virol. 71:8299-8306, 1997). Here, we test the ability of RID to protect human lymphocytes from apoptosis induced by ligation of Fas, a mechanism important for regulating lymphocyte populations. Using a retrovirus expressing RID to infect six human lymphocyte cell lines, we found that RID functions in the absence of other viral proteins to downregulate surface Fas on some, but not all, cell lines. Total cellular levels of Fas decrease as measured by Western blotting, and this loss of Fas correlates with protection from apoptosis induced by ligation of Fas in every cell line tested. Although in some cases, RID causes loss of only a fraction of surface Fas, the presence of RID completely blocks the immediate events downstream of Fas ligation (i.e., Fas-FADD association and caspase-8 cleavage) in susceptible cell lines. Nonetheless, the ability of RID to block Fas signaling is independent of the Fas signaling pathway used (type I or type II). Interestingly, among the four T-cell lines tested, RID caused loss of Fas in the two T-cell lines bearing a relatively immature phenotype, while having no activity in T cells with mature phenotypes. Collectively, these data suggest that RID functions to prevent apoptosis of some human lymphocytes by internalizing surface Fas receptors. It is possible that the expression of RID facilitates long-term infection by preventing Fas-mediated deletion of persistently infected lymphocytes.
journal_name
J Viroljournal_title
Journal of virologyauthors
McNees AL,Garnett CT,Gooding LRdoi
10.1128/jvi.76.19.9716-9723.2002subject
Has Abstractpub_date
2002-10-01 00:00:00pages
9716-23issue
19eissn
0022-538Xissn
1098-5514journal_volume
76pub_type
杂志文章abstract::Gene transfer to differentiated airway epithelia with existing viral vectors is very inefficient when they are applied to the apical surface. This largely reflects the polarized distribution of receptors on the basolateral surface. To identify new receptor-ligand interactions that might be used to redirect vectors to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.19.9234-9239.2000
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abstract::In order to study retroviral variation, selection, and viral correlates of in vivo pathogenicity, we documented the evolution of feline leukemia virus (FeLV) variants in cats that died with thymic lymphoma after infection with molecularly cloned subgroup A FeLV. Using genomic DNA from cat necropsy samples, we employed...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.4.2458-2467.1994
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.8.5395-5404.1996
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abstract::A porcine rotavirus (prv), variant 4F, isolated in tissue culture from the feces of a Chinese pig with diarrhea, was found to have become highly pathogenic when passaged in gnotobiotic piglets (J. C. Bridger, B. Burke, G. M. Beards, and U. Desselberger, J. Gen. Virol. 73:3011-3015, 1992). Comparison with the closely r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.853-857.1997
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.22.3.839-843.1977
更新日期:1977-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.9.5550-5559.1995
更新日期:1995-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.6.2730-2738.2002
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abstract::Interactions between host factors and the viral replication complex play important roles in host adaptation and regulation of influenza virus replication. A cellular protein, nuclear factor 90 (NF90), was copurified with H5N1 viral nucleoprotein (NP) from human cells in which NP was transiently expressed and identifie...
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.4.812-819.1975
更新日期:1975-04-01 00:00:00
abstract::Feline panleukopenia virus (FPV) and its host range variant, canine parvovirus (CPV), can bind the feline transferrin receptor (TfR), while only CPV binds to the canine TfR. Introducing two CPV-specific changes into FPV (at VP2 residues 93 and 323) endowed that virus with the canine TfR binding property and allowed ca...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.18.10099-10105.2003
更新日期:2003-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02723-07
更新日期:2008-06-01 00:00:00
abstract::The hepatitis B virus X protein acts as a transcriptional transactivator in vitro. To elucidate possible biological effects of X protein on liver cells in vivo, we generated four lines of transgenic mice carrying the X gene open reading frame under the control of the human alpha-1-antitrypsin regulatory region. The pl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.12.5939-5947.1990
更新日期:1990-12-01 00:00:00
abstract::The immediate-early gene ie2 of murine cytomegalovirus was characterized. The 1.75-kb ie2 transcript is spliced from three exons, of 78, 124, and 1,283 nucleotides, which are separated by introns of 1,245 and 364 nucleotides. An ATG codon located in the third exon leads into an open reading frame of 391 codons. Immedi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.3.1638-1643.1991
更新日期:1991-03-01 00:00:00
abstract::Our previous studies showed that establishment of murine cytomegalovirus (MCMV) latency in vivo is associated with repression of immediate-early gene expression, deacetylation of histones bound to the major immediate-early promoter (MIEP), changes in patterns of methylation of histones, and recruitment of cellular rep...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02380-09
更新日期:2010-04-01 00:00:00
abstract::Measles virus (MV) infection causes an acute childhood disease, associated in certain cases with infection of the central nervous system and development of a severe neurological disease. We have generated transgenic mice ubiquitously expressing the human protein SLAM (signaling lymphocytic activation molecule), or CD1...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00209-06
更新日期:2006-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.34.1.280-284.1980
更新日期:1980-04-01 00:00:00
abstract::Several different forms of progeny viral DNA can be identified in polyoma virus (Py)-infected mouse L-cells. The majority comprise mature form I superhelical DNA and the circular, double-stranded "theta" replicating intermediates in which the progeny DNA strands never exceed the unit genome length of the template. The...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.3.768-777.1983
更新日期:1983-06-01 00:00:00
abstract::The infectious entry pathway of duck hepatitis B virus (DHBV) was investigated with primary duck hepatocytes. Virus uptake was measured by a selective PCR technique which allows for the detection of a successful infection without the need for viral replication or gene expression. To test whether DHBV uptake occurs by ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.5827-5831.1996
更新日期:1996-09-01 00:00:00
abstract::Fine structure marker rescue involving the use of subfragments of vaccinia virus HindIII DNA fragments L, J, and H has been used to map the mutations in eight temperature-sensitive mutants of vaccinia virus representing four complementation groups. Comparison of their map locations with the positions of the open readi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.3.1027-1029.1985
更新日期:1985-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.2.319-324.1984
更新日期:1984-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.17.8797-8807.2002
更新日期:2002-09-01 00:00:00
abstract::Adult T-cell leukemia (ATL) occurs in a small population of human T-cell leukemia virus type 1 (HTLV-1)-infected individuals. Although the critical risk factor for ATL development is not clear, it has been noted that ATL is incidentally associated with mother-to-child infection, elevated proviral loads, and weakness i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.5.2956-2963.2003
更新日期:2003-03-01 00:00:00
abstract::We have previously shown that styrylquinolines (SQLs) are integrase inhibitors in vitro. They compete with the long terminal repeat substrate for integrase. Here, we describe the cellular mode of action of these molecules. We show that SQLs do not interfere with virus entry. In fact, concentrations of up to 20 times t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.11.5728-5736.2004
更新日期:2004-06-01 00:00:00
abstract::Replication and transcription activator (RTA) (also referred to as ORF50), an immediate-early gene product of Kaposi's sarcoma-associated herpesvirus (KSHV)/(human herpesvirus 8), plays a critical role in balancing the viral life cycle between latency and lytic replication. RTA has been shown to act as a strong transc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.17.9451-9462.2003
更新日期:2003-09-01 00:00:00
abstract::We have reinvestigated the subcellular distribution of the duck hepatitis B virus (DHBV) core protein in infected duck hepatocytes and in transfected cells. By using indirect immunofluorescence, the protein was found to be localized not only in the cytoplasm, as described previously, but also within the cell nucleus, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.3.2157-2164.2003
更新日期:2003-02-01 00:00:00
abstract::Murine cytomegalovirus encodes three regulators of antigen presentation to antiviral CD8 T cells. According to current paradigms, all three regulators are committed to the inhibition of the presentation of antigenic peptides. Whereas m152/gp40 catalyzes the retention of peptide-loaded major histocompatibility complex ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00723-06
更新日期:2006-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.3.1274-1283.2001
更新日期:2001-02-01 00:00:00
abstract::Secondary cultures of chicken embryo fibroblasts were infected with the Schmidt Ruppin strain of Rous sarcoma virus (RSV). Five days after infection, the medium was replaced at 2-h intervals with phosphate-free Eagle medium containing 50 muCi of [32P]orthophosphate per ml. Virus was collected by centrifugation, and th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.1.161-166.1975
更新日期:1975-01-01 00:00:00
abstract::Human norovirus (HuNoV) is a major causative agent of food-borne gastroenteritis worldwide. Currently, there are no vaccines or effective therapeutic interventions for this virus. Development of an attenuated vaccine for HuNoV has been hampered by the inability to grow the virus in cell culture. Thus, a vector-based v...
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更新日期:2011-03-01 00:00:00
abstract:UNLABELLED:Multiple cellular pathways are regulated by small ubiquitin-like modifier (SUMO) modification, including ubiquitin-mediated proteolysis, signal transduction, innate immunity, and antiviral defense. In the study described in this report, we investigated the effects of SUMO on the replication of two members of...
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pub_type: 杂志文章
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更新日期:2016-06-24 00:00:00