Fine structure marker rescue of temperature-sensitive mutations of vaccinia virus within a central conserved region of the genome.

abstract：:The Vpr protein, encoded by the human immunodeficiency virus type 1 (HIV-1) genome, is one of the nonstructural proteins packaged in large amounts into viral particles. We have previously reported that Vpr associates with the DNA repair enzyme uracil DNA glycosylase (UDG). In this study, we extended these observations...

journal_title：Journal of virology

authors： Willetts KE,Rey F,Agostini I,Navarro JM,Baudat Y,Vigne R,Sire J

更新日期：1999-02-01 00:00:00

abstract：:A specific endoribonucleolytic activity was detected when detergent-lysed vesicular stomatitis of Sendai virus was incubated with the precursor to Escherichia coli tRNA Tyr. The cleavage products produced and the characteristics of the reaction were similar to those previously reported for human KB cell RNase NU. Like...

journal_title：Journal of virology

authors： Kolakofsky D,Altman S

更新日期：1978-01-01 00:00:00

abstract：:The development of resistance to host defense mechanisms such as tumor necrosis factor (TNF)- and Fas-mediated apoptosis of transformed or virus-infected cells may be a critical component in the development of disease. To find genes that protect cells from apoptosis, we used an expression cloning strategy and identifi...

journal_title：Journal of virology

authors： Foghsgaard L,Jäättelä M

更新日期：1997-10-01 00:00:00

abstract：:The occurrence of genome segment reassortment between two antigenically related orbiviruses was demonstrated in cattle. Individual virus clones were isolated by cell culture plaque assays directly from the blood of a calf infected with two serotypes of bluetongue virus. The majority (89%) of progeny viruses isolated f...

journal_title：Journal of virology

authors： Stott JL,Oberst RD,Channell MB,Osburn BI

更新日期：1987-09-01 00:00:00

abstract：:Kaposi's sarcoma (KS) and primary effusion lymphoma (PEL) cells are predominantly infected with latent Kaposi's sarcoma-associated herpesvirus (KSHV), presenting a barrier to the destruction of tumor cells. Latent KSHV can be reactivated to undergo lytic replication. Here we report that in PEL cells, oxidative stress ...

journal_title：Journal of virology

authors： Li X,Feng J,Sun R

更新日期：2011-01-01 00:00:00

abstract：:The major immediate-early (MIE) transactivator proteins of cytomegaloviruses (CMV) play a pivotal role in the initiation of virus-host cell interactions. Therefore, cis- and trans-acting factors influencing the expression of these proteins through their upstream promoter-enhancer regions are important determinants of ...

journal_title：Journal of virology

authors： Chang YN,Jeang KT,Chiou CJ,Chan YJ,Pizzorno M,Hayward GS

更新日期：1993-01-01 00:00:00

abstract：:African swine fever virus induces in convalescent pigs antibodies that neutralized the virus before and after binding to susceptible cells, inhibiting both virus attachment and internalization. A further analysis of the neutralization mechanisms mediated by the different viral proteins showed that antibodies to protei...

journal_title：Journal of virology

authors： Gómez-Puertas P,Rodríguez F,Oviedo JM,Ramiro-Ibáñez F,Ruiz-Gonzalvo F,Alonso C,Escribano JM

更新日期：1996-08-01 00:00:00

abstract：:Members of the Flaviviridae encode a serine proteinase termed NS3 that is responsible for processing at several sites in the viral polyproteins. In this report, we show that the NS3 proteinase of the pestivirus bovine viral diarrhea virus (BVDV) (NADL strain) is required for processing at nonstructural (NS) protein si...

journal_title：Journal of virology

authors： Xu J,Mendez E,Caron PR,Lin C,Murcko MA,Collett MS,Rice CM

更新日期：1997-07-01 00:00:00

abstract：:The multiplicity of attachment (MOA) of a virion in any particular time interval is the average number of cellular attachment opportunities that must be blocked to keep the virion in suspension. MOA is usually proportional to incubation time and cell concentration. Low MOA (like low multiplicity of infection) is requi...

journal_title：Journal of virology

authors： Spouge JL

更新日期：1994-03-01 00:00:00

abstract：:Carcinogenic progression of a human papillomavirus (HPV)-infected cell is often associated with integration of the viral genome in a manner which results in the loss of expression of the viral regulatory protein E2. One function of E2 is the regulation of expression of the viral oncogenes, E6 and E7. Introduction of t...

journal_title：Journal of virology

authors： Dowhanick JJ,McBride AA,Howley PM

更新日期：1995-12-01 00:00:00

abstract：:Retroviruses use RNA as their genetic material within viral particles and DNA (provirus) as their genetic material within cells. The rate of recombination during reverse transcription between two identical sequences within the same RNA molecule is very high. In this study, we have developed a sensitive system to study...

journal_title：Journal of virology

authors： Li T,Zhang J

更新日期：2000-08-01 00:00:00

abstract：UNLABELLED:Bean pod mottle virus (BPMV) is a bipartite, positive-sense (+) RNA plant virus in the Secoviridae family. Its RNA1 encodes proteins required for genome replication, whereas RNA2 primarily encodes proteins needed for virion assembly and cell-to-cell movement. However, the function of a 58-kDa protein (P58) e...

journal_title：Journal of virology

authors： Lin J,Guo J,Finer J,Dorrance AE,Redinbaugh MG,Qu F

更新日期：2014-03-01 00:00:00

abstract：:Human metapneumovirus (hMPV) is a leading cause of viral lower respiratory tract infection in children. The sole target of neutralizing antibodies targeting hMPV is the fusion (F) protein, a class I viral fusion protein mediating virus-cell membrane fusion. There have been several monoclonal antibodies (mAbs) isolated...

journal_title：Journal of virology

authors： Bar-Peled Y,Diaz D,Pena-Briseno A,Murray J,Huang J,Tripp RA,Mousa JJ

更新日期：2019-09-12 00:00:00

abstract：:The involvement of two bacteriophage T4 gene products in the initiation of T4 tail tube and sheath polymerization on mature baseplates has been studied by radioautography of acrylamide gels of various partially completed tail structures. The products of genes 48 and 54 (P48[the nomenclature P48 refers to the protein p...

journal_title：Journal of virology

authors： Berget PB,Warner HR

更新日期：1975-12-01 00:00:00

abstract：:Coupling of G proteins to ligand-engaged chemokine receptors is the paramount event in G-protein-coupled receptor signal transduction. Previously, we have demonstrated that the human cytomegalovirus-encoded chemokine receptor US28 mediates human vascular smooth muscle cell (SMC) migration in response to either RANTES ...

journal_title：Journal of virology

authors： Melnychuk RM,Streblow DN,Smith PP,Hirsch AJ,Pancheva D,Nelson JA

更新日期：2004-08-01 00:00:00

abstract：:Virus was isolated from the lumen of the calyx region of ovaries in the parasitoid wasp Campoletis sonorensis (Hymenoptera: Ichneumonidae), and the nature of the viral DNA was analyzed. DNA purified from a homogeneous band of virus contained double-stranded superhelical molecules which were polydisperse in molecular w...

journal_title：Journal of virology

authors： Krell PJ,Summers MD,Vinson SB

更新日期：1982-09-01 00:00:00

abstract：:Brome mosaic virus (BMV), a member of the alphavirus-like super-family of positive-strand RNA viruses, encodes two proteins required for viral RNA replication: 1a and 2a. 1a contains m7G methyltransferase- and helicase-like domains, while 2a contains a polymerase (pol)-like core flanked by N- and C-terminal extensions...

journal_title：Journal of virology

authors： Smirnyagina E,Lin NS,Ahlquist P

更新日期：1996-07-01 00:00:00

abstract：:In mammalian cells, alphavirus replication complexes are anchored to the plasma membrane. This interaction with lipid bilayers is mediated through the viral methyl/guanylyltransferase nsP1 and reinforced by palmitoylation of cysteine residue(s) in the C-terminal region of this protein. Lipid content of membranes suppo...

journal_title：Journal of virology

authors： Bakhache W,Neyret A,Bernard E,Merits A,Briant L

更新日期：2020-05-04 00:00:00

abstract：:The human cytomegalovirus UL47 open reading frame encodes a 110-kDa protein that is a component of the virion tegument. We have constructed a cytomegalovirus mutant, ADsubUL47, in which the central portion of the UL47 open reading frame has been replaced by two marker genes. The mutant replicated to titers 100-fold lo...

journal_title：Journal of virology

authors： Bechtel JT,Shenk T

更新日期：2002-02-01 00:00:00

abstract：:We investigated the features of the Moloney murine sarcoma virus leader sequence necessary for RNA packaging function by using a deletion analysis approach. We found that sequences that extend beyond those characterized genetically in previous reports are important for optimal packaging efficiency. A fragment covering...

journal_title：Journal of virology

authors： Izmailova E,Aldovini A

更新日期：2002-05-01 00:00:00

abstract：:A phage, designated CP-51, that carries out generalized transduction in Bacillus cereus 569 was isolated from soil. All auxotrophic mutants tested, those requiring tryptophan, histidine, leucine, isoleucine, methionine, or phenylalanine, were transduced to prototrophy. The phage was extremely unstable when stored at 2...

journal_title：Journal of virology

authors： Thorne CB

更新日期：1968-07-01 00:00:00

abstract：:The complete nucleotide sequence of the NS mRNA of vesicular stomatitis virus (New Jersey serotype) was established from two cDNA clones spanning the entire coding region of the mRNA. The gene is 856 nucleotides long and can code for a polypeptide of 274 amino acids. Comparison with the nucleotide sequence of the NS g...

journal_title：Journal of virology

authors： Gill DS,Banerjee AK

更新日期：1985-07-01 00:00:00

abstract：:In an attempt to generate broadly cross-reactive, neutralizing monoclonal antibodies (MAbs) to simian immunodeficiency virus (SIV), we compared two immunization protocols using different preparations of oligomeric SIV envelope (Env) glycoproteins. In the first protocol, mice were immunized with soluble gp140 (sgp140) ...

journal_title：Journal of virology

authors： Edinger AL,Ahuja M,Sung T,Baxter KC,Haggarty B,Doms RW,Hoxie JA

更新日期：2000-09-01 00:00:00

abstract：:Human immunodeficiency virus type 1 (HIV-1)-infected macrophages damage mature neurons in the brain, although their effect on neuronal development has not been clarified. In this study, we show that HIV-1-infected macrophages produce factors that impair the development of neuronal precursor cells and that soluble vira...

journal_title：Journal of virology

authors： Kitayama H,Miura Y,Ando Y,Hoshino S,Ishizaka Y,Koyanagi Y

更新日期：2008-03-01 00:00:00

abstract：:The agent(s) responsible for sporadic non-A, non-B hepatitis in humans was serially transmitted in rhesus monkeys by intravenous inoculation of the stool extract from a patient. A novel agent called HFV (hepatitis French [origin] virus) was present as 27- to 37-nm particles in the infectious stool extract. Hepatopathi...

journal_title：Journal of virology

authors： Deka N,Sharma MD,Mukerjee R

更新日期：1994-12-01 00:00:00

abstract：:In this report, we describe some phenotypic properties of a temperature-sensitive mutant of herpes simplex type 1 (HSV-1) and present data concerning the physical location and nucleotide sequence of the genomic region harboring the mutation. The effect of shifts from the permissive to the nonpermissive temperature on ...

journal_title：Journal of virology

authors： Pertuiset B,Boccara M,Cebrian J,Berthelot N,Chousterman S,Puvion-Dutilleul F,Sisman J,Sheldrick P

更新日期：1989-05-01 00:00:00

abstract：:In eukaryotic cells, cell growth and division occur in a stepwise, orderly fashion described by a process known as the cell cycle. The relationship between positive-strand RNA viruses and the cell cycle and the concomitant effects on virus replication are not clearly understood. We have shown that infection of asynchr...

journal_title：Journal of virology

authors： Dove B,Brooks G,Bicknell K,Wurm T,Hiscox JA

更新日期：2006-04-01 00:00:00

abstract：:We present an antiviral-immunomodulatory therapeutic strategy involving the chemokine receptor antagonist Met-RANTES, which yields significant survival in the setting of an otherwise fatal respiratory virus infection. In previous work, we demonstrated that infection with the natural rodent pathogen pneumonia virus of ...

journal_title：Journal of virology

authors： Bonville CA,Lau VK,DeLeon JM,Gao JL,Easton AJ,Rosenberg HF,Domachowske JB

更新日期：2004-08-01 00:00:00

abstract：:Isoelectrofocusing of nonionic-detergent-disrupted flaviviruses separated the envelope glycoprotein of 53,000 to 58,000 daltons and the nucleocapsid protein of 14,000 daltons. The envelope protein and nucleocapsid protein were isolated at isoelectric points of pI 7.8 and 10.3, respectively. The antigenic determinants ...

journal_title：Journal of virology

authors： Trent DW

更新日期：1977-06-01 00:00:00

abstract：UNLABELLED:The DNA damage response (DDR) that evolved to repair host cell DNA damage also recognizes viral DNA entering the nucleus during infections. Here, we investigated the modulation of DDR signaling during de novo infection of primary endothelial cells by Kaposi's sarcoma-associated herpesvirus (KSHV). Phosphoryl...

journal_title：Journal of virology

authors： Singh VV,Dutta D,Ansari MA,Dutta S,Chandran B

更新日期：2014-03-01 00:00:00