Abstract:
:We present an antiviral-immunomodulatory therapeutic strategy involving the chemokine receptor antagonist Met-RANTES, which yields significant survival in the setting of an otherwise fatal respiratory virus infection. In previous work, we demonstrated that infection with the natural rodent pathogen pneumonia virus of mice involves robust virus replication accompanied by cellular inflammation modulated by the CC chemokine macrophage inflammatory protein 1alpha (MIP-1alpha). We found that the antiviral agent ribavirin limited virus replication in vivo but had no impact on morbidity and mortality associated with this disease in the absence of immunomodulatory control. We show here that ribavirin reduces mortality, from 100% to 10 and 30%, respectively, in gene-deleted CCR1(-/-) mice and in wild-type mice treated with the small-molecule chemokine receptor antagonist, Met-RANTES. As MIP-1alpha-mediated inflammation is a common response to several distantly related respiratory virus pathogens, specific antiviral therapy in conjunction with blockade of the MIP-1alpha/CCR1 inflammatory cascade may ultimately prove to be a useful, generalized approach to severe respiratory virus infection and its pathological sequelae in human subjects.
journal_name
J Viroljournal_title
Journal of virologyauthors
Bonville CA,Lau VK,DeLeon JM,Gao JL,Easton AJ,Rosenberg HF,Domachowske JBdoi
10.1128/JVI.78.15.7984-7989.2004subject
Has Abstractpub_date
2004-08-01 00:00:00pages
7984-9issue
15eissn
0022-538Xissn
1098-5514pii
78/15/7984journal_volume
78pub_type
杂志文章abstract::Koi herpesvirus (KHV) is the causative agent of a lethal disease in koi and common carp. In the present study, we describe the cloning of the KHV genome as a stable and infectious bacterial artificial chromosome (BAC) clone that can be used to produce KHV recombinant strains. This goal was achieved by the insertion of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00211-08
更新日期:2008-05-01 00:00:00
abstract::For efficient and versatile use of adenovirus (Ad) as an in vivo gene therapy vector, modulation of the viral tropism is highly desirable. In this study, a novel method to genetically alter the Ad fiber tropism is described. The knob and the last 15 shaft repeats of the fiber gene were deleted and replaced with an ext...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.16.7280-7289.2001
更新日期:2001-08-01 00:00:00
abstract::The development of vaccines against bluetongue, a prevalent livestock disease, has been focused on surface antigens that induce strong neutralizing antibody responses. Because of their antigenic variability, these vaccines are usually serotype restricted. We now show that a single highly conserved nonstructural protei...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00938-18
更新日期:2018-07-31 00:00:00
abstract::Membrane-containing extracts isolated from tobacco plants infected with the plus-strand RNA virus, potato virus X (PVX), supported synthesis of four major, high-molecular-weight PVX RNA products (R1 to R4). Nuclease digestion and hybridization studies indicated that R1 and R2 are a mixture of partially single-stranded...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.7.4795-4799.1996
更新日期:1996-07-01 00:00:00
abstract::Upon inoculation into AKR mice, mink cell focus-forming murine leukemia virus (MCF MLV) accelerates thymic lymphoma formation. During the preleukemic phase of disease, we observed the induction of apoptosis in thymic lymphocytes. A similar induction of apoptosis was observed for cultured mink epithelial cells after MC...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.10.5088-5096.2004
更新日期:2004-05-01 00:00:00
abstract::We assessed the prediction that access of the viral NS1 protein to cellular PDZ domain protein networks enhances the virulence of highly pathogenic avian influenza A viruses. The NS1 proteins of most avian influenza viruses bear the C-terminal ligand sequence Glu-Ser-Glu-Val (ESEV) for PDZ domains present in multiple ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00610-10
更新日期:2010-10-01 00:00:00
abstract::Friend virus induces an erythroleukemia in susceptible mice that is initiated by the interaction of the Friend virus-encoded glycoprotein gp55 with the erythropoietin (Epo) receptor and the product of the host Fv2 gene, a naturally occurring truncated form of the Stk receptor tyrosine kinase (Sf-Stk). We have previous...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02090-09
更新日期:2010-03-01 00:00:00
abstract::Inbred LSH hamster brain cells were transformed in vitro by the GS strain of BK virus (BKV), and transplantable tumors classified as undifferentiated glioblastomas were induced in the syngeneic host. The viral status in the transformed cells, designated LSH-BR-BK, was established. About 46 genome equivalents per cell ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.40.1.276-284.1981
更新日期:1981-10-01 00:00:00
abstract::To evaluate host range differences between two different strains of feline leukemia virus subgroup B (FeLV-B), we compared the binding and infectivity patterns of retrovirus vectors bearing either FeLV-B-90Z or FeLV-B-GA envelopes. We report here that the ability of these envelopes to utilize different Pit1 orthologs ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.23.12369-12375.2002
更新日期:2002-12-01 00:00:00
abstract::Human adenoviruses (HAdV) are nonenveloped viruses containing a linear, double-stranded DNA genome surrounded by an icosahedral capsid. To allow proper viral replication, the genome is imported through the nuclear pore complex associated with viral core proteins. Until now, the role of these incoming virion proteins d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01451-17
更新日期:2018-01-30 00:00:00
abstract::H5N1 and H9N2 avian influenza virus subtypes top the World Health Organization's list for the greatest pandemic potential. Inactivated H5N1 vaccines induce limited immune responses and, in the case of live-attenuated influenza virus vaccines (LAIV), there are safety concerns regarding the possibility of reassortment b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02490-12
更新日期:2013-05-01 00:00:00
abstract::NS4B is one of the nonstructural proteins of classical swine fever virus (CSFV), the etiological agent of a severe, highly lethal disease of swine. Protein domain analysis of the predicted amino acid sequence of the NS4B protein of highly pathogenic CSFV strain Brescia (BICv) identified a putative Toll/interleukin-1 r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02050-09
更新日期:2010-02-01 00:00:00
abstract::We have previously shown for the paramyxovirus simian virus 5 (SV5) that a functional promoter for RNA replication requires proper spacing between two discontinuous elements: a 19-base segment at the 3' terminus (conserved region I [CRI]) and an 18-base internal region (CRII) that is contained within the coding region...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.8.3993-3998.2001
更新日期:2001-04-01 00:00:00
abstract::The structural aspects of SH antigen-containing particles were investigated. These studies confirmed the existence of a large spherical particle (ca. 43 nm) and smaller (ca. 20 nm) rod- and sphere-shaped particles. The large particle consists of an outer and inner membrane and a core of "nucleic acid" as seen by posit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.6.5.685-689.1970
更新日期:1970-11-01 00:00:00
abstract::The Vif protein of human immunodeficiency virus type 1 (HIV-1) is important for virion infectivity. Previous studies have shown that vif-defective virions exhibit structural abnormalities in the virus core and are defective in the ability to complete proviral DNA synthesis in acutely infected cells. We developed novel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.23.11055-11066.2000
更新日期:2000-12-01 00:00:00
abstract::Measles virus (MeV) infection is characterized by the formation of multinuclear giant cells (MGC). We report that beta interferon (IFN-beta) production is amplified in vitro by the formation of virus-induced MGC derived from human epithelial cells or mature conventional dendritic cells. Both fusion and IFN-beta respon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00078-07
更新日期:2007-12-01 00:00:00
abstract::The morphology and development of four members of the reticuloendotheliosis virus group were studied by transmission electron microscopy. Virions of duck spleen necrosis virus, duck infectious anemia virus, chicken syncytial virus, and reticuloendotheliosis virus strain T are sperical with a diameter of approximately ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.4.1027-1038.1975
更新日期:1975-10-01 00:00:00
abstract::When residues 17 and 18 in nef of simian immunodeficiency virus strain SIVmac239 were changed from RQ to YE, the resultant virus was able to replicate in peripheral blood mononuclear cell cultures without prior lymphocyte activation and without the addition of exogenous interleukin-2, caused extensive lymphocyte activ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.6.4157-4161.1996
更新日期:1996-06-01 00:00:00
abstract::Hepatitis B virus (HBV) and woolly monkey hepatitis B virus (WMHBV) are primate hepadnaviruses that display restricted tissue and host tropisms. Hepatitis D virus (HDV) particles pseudotyped with HBV and WMHBV envelopes (HBV-HDV and WM-HDV) preferentially infect human and spider monkey hepatocytes, respectively, there...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.15.9786-9798.2005
更新日期:2005-08-01 00:00:00
abstract::Glycoprotein H (gH) is conserved among all herpesviruses and is essential for virus entry and cell fusion along with gL, gB, and, in most alphaherpesviruses, gD. Within the gH/gL heterodimer, it is thought that gH accounts for the fusion function and gL acts as a chaperone for the folding and transport of gH. Here, we...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00097-07
更新日期:2007-05-01 00:00:00
abstract::Rotavirus is the leading global cause of diarrheal mortality for unvaccinated children under 5 years of age. The outer capsid of rotavirus virions consists of VP7 and VP4 proteins, which determine viral G and P types, respectively, and are primary targets of neutralizing antibodies. Successful vaccination depends upon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01476-18
更新日期:2018-12-10 00:00:00
abstract::Duck hepatitis B virus mutants containing frameshift or stop codon mutations in a portion of the viral pol gene separating the terminal protein and reverse transcriptase domains had a leaky phenotype and, depending on the location and type of mutation, synthesized up to 10% as much viral DNA as did the wild type. This...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.5.2155-2163.1991
更新日期:1991-05-01 00:00:00
abstract::Vaccine-induced memory is necessary for protective immunity to pathogens, but many viruses induce a state of transient immune suppression that might contribute to the inability of a vaccine to elicit immunity. We evaluated here the fate of bystander T cells activated by third party cognate antigens during acute viral ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02516-10
更新日期:2011-06-01 00:00:00
abstract::We have studied initiation and regulation of early transcription of simian virus (SV40) DNA in vitro by eucaryotic RNA polymerase II, using both a crude HeLa cell extract and a partially purified calf thymus polymerase supplemented with a HeLa cell S100 fraction. Analysis of initiation sites by primer-directed cDNA sy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.2.449-461.1982
更新日期:1982-02-01 00:00:00
abstract::Merkel cell polyomavirus (MCV) is a recently discovered human polyomavirus causing the majority of human Merkel cell carcinomas. We mapped a 71-bp minimal MCV replication core origin sufficient for initiating eukaryotic DNA replication in the presence of wild-type MCV large T protein (LT). The origin includes a poly(T...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01336-09
更新日期:2009-12-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) accessory protein Nef is heavily targeted by CD8(+) T lymphocytes (CTLs) during acute infection and therefore is included in many candidate vaccines. We investigated whether CTL targeting of Nef during acute infection contributes to immune control by disrupting the funct...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00482-14
更新日期:2014-07-01 00:00:00
abstract::HTG2 hamster cells produce a defective murine sarcoma virus lacking gp70 and, consequently, viral surface projections (knobs), but the lack of knobs appears to have no effect on intramembrane particle distribution. In addition, it has been noted that the core of the virus remains in the "immature" form as a result of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.3.872-877.1980
更新日期:1980-12-01 00:00:00
abstract::Complementary DNA clones encoding the murine homolog (mCAR) of the human coxsackievirus and adenovirus receptor (CAR) were isolated. Nonpermissive CHO cells transfected with mCAR cDNA became susceptible to infection by coxsackieviruses B3 and B4 and showed increased susceptibility to adenovirus-mediated gene transfer....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.415-419.1998
更新日期:1998-01-01 00:00:00
abstract::We have cloned several prototypic members of the family of human endogenous retroviruslike elements having a histidine tRNA primer-binding site (RTVL-H) and have determined the nucleotide sequence of one of these clones (RTVL-H2). The RTVL-H2 sequence is 5,813 nucleotides long, with long terminal repeats of 450 nucleo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.12.4060-4066.1987
更新日期:1987-12-01 00:00:00
abstract::Since most human immunodeficiency virus (HIV) infections are initiated following mucosal exposure to the virus, the anatomic containment or abortion of an HIV infection is likely to require vaccine-elicited cellular immune responses in those mucosal sites. Studying vaccine-elicited mucosal immune responses has been pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.22.11484-11490.2002
更新日期:2002-11-01 00:00:00