Abstract:
:Kaposi's sarcoma (KS) and primary effusion lymphoma (PEL) cells are predominantly infected with latent Kaposi's sarcoma-associated herpesvirus (KSHV), presenting a barrier to the destruction of tumor cells. Latent KSHV can be reactivated to undergo lytic replication. Here we report that in PEL cells, oxidative stress induced by upregulated reactive oxygen species (ROS) can lead to KSHV reactivation or cell death. ROS are upregulated by NF-κB inhibition and are required for subsequent KSHV reactivation. Disruption of the intracellular redox balance through depletion of the antioxidant glutathione or inhibition of the antioxidant enzyme catalase also induces KSHV reactivation, suggesting that hydrogen peroxide induces reactivation. In addition, p38 signaling is required for KSHV reactivation induced by ROS. Furthermore, treatment of PEL cells with a higher concentration of the NF-κB inhibitor than that used for inducing KSHV reactivation further upregulates ROS and induces massive cell death. ROS, but not p38 signaling, are required for PEL cell death induced by NF-κB inhibition as well as by glutathione depletion. Importantly, anticancer drugs, such as cisplatin and arsenic trioxide, also induce KSHV reactivation and PEL cell death in a ROS-dependent manner. Our study thus establishes a critical role for ROS and oxidative stress in the regulation of KSHV reactivation and PEL cell death. Disrupting the cellular redox balance may be a potential strategy for treating KSHV-associated lymphoma.
journal_name
J Viroljournal_title
Journal of virologyauthors
Li X,Feng J,Sun Rdoi
10.1128/JVI.01742-10subject
Has Abstractpub_date
2011-01-01 00:00:00pages
715-24issue
2eissn
0022-538Xissn
1098-5514pii
JVI.01742-10journal_volume
85pub_type
杂志文章abstract::Defensins are innate immune effector peptides expressed at mucosal surfaces throughout the human body and are potently antiviral in vitro The role of defensins in viral pathogenesis in vivo is poorly understood; however, recent studies have revealed that defensin-virus interactions in vivo are complicated and distinct...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.00904-15
更新日期:2016-05-12 00:00:00
abstract::There is an open reading frame in the E3 transcription unit of adenovirus type 3 (Ad3) and Ad7 that could encode a protein of 16 kDa (16K protein). Ad3 and Ad7 are members of subgroup B of human adenoviruses. Using a rabbit antipeptide antiserum, we show that the 16K protein is expressed in Ad3- and Ad7-infected cells...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.7.4292-4298.1995
更新日期:1995-07-01 00:00:00
abstract::Adenovirus type 2 (Ad2) and Ad5 enter epithelial cells via the coxsackievirus B Ad receptor (CAR) and alpha(v) integrin coreceptors. In the absence of CAR, they can be directed to the Fcgamma receptor 1 of hematopoietic cells by an adaptor comprising the extracellular CAR domain and the Fc portion of a human immunoglo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.4.2604-2613.2005
更新日期:2005-02-01 00:00:00
abstract::The immunosuppressant drug rapamycin blocks progression of the cell cycle at G1 in mammalian cells and yeast. We recently showed that rapamycin inhibits both in vitro and in vivo cap-dependent, but not cap-independent, translation. This inhibition is causally related to reduced phosphorylation and consequent activatio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.12.8993-8996.1996
更新日期:1996-12-01 00:00:00
abstract::Viral DNA sequences were not detected in high-molecular-weight host DNA until well after the onset of viral DNA replication. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.30.1.397-399.1979
更新日期:1979-04-01 00:00:00
abstract::Closed circular unintegrated DNA of the SEATO strain of gibbon ape leukemia virus (GaLV-S) was isolated from canine thymus fibroblasts after cocultivation with chronically infected bat lung fibroblasts. Restriction endonuclease HindIII cleaves GaLV-S DNA once, thus allowing isolation and cloning of HindIII-digested un...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.1.269-275.1982
更新日期:1982-10-01 00:00:00
abstract::This paper reports the use of zonal ultracentrifuge techniques to conduct biophysical studies of rhinoviruses grown with WI-38 cells. Good clean-out of infectivity from rhinovirus harvests was obtained with the continuous-flow B-V and B-IX rotors. Use of the B-V rotor resulted in the successful concentration of rhinov...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.9.937-943.1968
更新日期:1968-09-01 00:00:00
abstract::The herpes simplex virus type 1 UL6 protein forms a 12-subunit ring structure at a unique capsid vertex which functions as a conduit for encapsidation of the viral genome. To characterize UL6 protein domains that are involved in intersubunit interactions and interactions with other capsid proteins, we engineered a set...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00739-07
更新日期:2007-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.3.1056-1067.1982
更新日期:1982-12-01 00:00:00
abstract::Type I interferon (IFN-I) inhibits the replication of different viruses. However, the effect of IFN-I on the human T-lymphotropic virus type 1 (HTLV-1) viral cycle is controversial. Here, we investigated the consequences of IFN-α addition for different steps of HTLV-1 and HTLV-2 infection. We first show that alpha int...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02758-13
更新日期:2013-12-01 00:00:00
abstract::The protease (PR) from human immunodeficiency virus (HIV) is essential for viral replication: this aspartyl protease, active only as a dimer, is responsible for cleavage of the viral polyprotein precursors (Gag and Gag-Pol), to release the functional mature proteins. In this work, we have studied the structure-functio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.15.8216-8226.2003
更新日期:2003-08-01 00:00:00
abstract::The B19 parvovirus, a small single-stranded DNA virus of 5.4 kilobases, is pathogenic in humans. B19 has remarkable specificity for erythroid progenitor cells and has been propagated in vitro only with human erythroid bone marrow. Replication of viral DNA and the viral protein products of B19 appear similar to those o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.8.2395-2406.1987
更新日期:1987-08-01 00:00:00
abstract::A hydrophilic region consisting of strikingly clustered charged amino acids is present at the center of human immunodeficiency virus type 1 (HIV-1) Vif. In this study, the role for this central hydrophilic region (E(88)WRKKR(93)) in the virus replication in nonpermissive H9 cells was investigated by extensive deletion...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.2.1626-1632.2003
更新日期:2003-01-01 00:00:00
abstract::A DNA vaccine plasmid containing the Japanese encephalitis (JE) virus premembrane (prM) and envelope (E) genes (designated pcDNA3JEME) was evaluated for immunogenicity and protective efficacy in mice. Two immunizations of 4-week-old female ICR mice with pcDNA3JEME by intramuscular or intradermal injections at a dose o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.6.4925-4930.1998
更新日期:1998-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.6.2936-2951.2002
更新日期:2002-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.1.198-205.1995
更新日期:1995-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.22.13943-13952.2005
更新日期:2005-11-01 00:00:00
abstract::The investigation of papillomavirus chromatin has been hampered by the unavailability of a tissue culture system for vegetative growth of these viruses. We have used, therefore, bovine papillomavirus type 1-transformed hamster embryo fibroblasts containing 200 to 250 episomal genome equivalents per cell as a source of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.2.567-574.1983
更新日期:1983-05-01 00:00:00
abstract:UNLABELLED:Cavally virus (CavV) and related viruses in the family Mesoniviridae diverged profoundly from other nidovirus lineages but largely retained the characteristic set of replicative enzymes conserved in the Coronaviridae and Roniviridae. The expression of these enzymes in virus-infected cells requires the extens...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02040-14
更新日期:2014-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.3.1486-1494.2000
更新日期:2000-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.1.47-54.1996
更新日期:1996-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.1.131-138.1977
更新日期:1977-01-01 00:00:00
abstract::Here, we report that specific manipulations of the cellular response to virus infection can cause prevention of apoptosis and consequent establishment of persistent infection. Infection of several human cell lines with Sendai virus (SeV) or human parainfluenza virus 3, two prototypic paramyxoviruses, caused slow apopt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02536-07
更新日期:2008-04-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) evokes a strong immune response, but the virus persists. Polymorphisms within known antigenic sites result in loss of immune recognition and can be positively selected. Amino acid variation outside known HLA class I restricted epitopes can also enable immune escape by interf...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00968-06
更新日期:2007-02-01 00:00:00
abstract::Treatment of human immunodeficiency virus type 1 (HIV-1)-infected CEM cell cultures with escalating concentrations of the quinoxaline S-2720 resulted in an ordered appearance of single and multiple mutant virus strains that gradually became resistant to the quinoxaline and other nonnucleoside reverse transcriptase (RT...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8195-8203.1997
更新日期:1997-11-01 00:00:00
abstract::A recent publication indicated that overexpression of Axl, a cellular receptor that negatively regulates Toll-like receptor signaling, enhanced the entry of viruses pseudotyped with the glycoprotein of lymphocytic choriomeningitis virus (LCMV) in vitro. In testing the biological relevance of these observations, we fou...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03268-12
更新日期:2013-04-01 00:00:00
abstract::Three molecules have been identified as the main cellular factors required for binding and entry of human T-cell leukemia virus type 1 (HTLV-1): glucose transporter 1 (GLUT1), heparan sulfate (HS), and neuropilin 1 (NRP-1). However, the precise mechanism of HTLV-1 cell tropism has yet to be elucidated. Here, we examin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05783-11
更新日期:2012-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.23.3.533-542.1977
更新日期:1977-09-01 00:00:00
abstract::Efforts to develop a vaccine against human immunodeficiency virus type 1 (HIV-1) are complicated by resistance of virus to neutralization. The neutralization resistance phenotype of HIV-1 has been linked to high infectivity. We studied the mechanisms determining this phenotype using clones of the T-cell-line-adapted (...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.1.560-570.2003
更新日期:2003-01-01 00:00:00
abstract::Lentiviruses are nononcogenic retroviruses that cause persistent infections and slowly progressive diseases. Visna virus, a lentivirus of sheep, persists in cells of the macrophage lineage despite the presence of neutralizing antibodies in the animal. These antibodies are measured by prevention of virus replication in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.59.1.37-44.1986
更新日期:1986-07-01 00:00:00