Abstract:
:Three molecules have been identified as the main cellular factors required for binding and entry of human T-cell leukemia virus type 1 (HTLV-1): glucose transporter 1 (GLUT1), heparan sulfate (HS), and neuropilin 1 (NRP-1). However, the precise mechanism of HTLV-1 cell tropism has yet to be elucidated. Here, we examined the susceptibilities of various human cell lines to HTLV-1 by using vesicular stomatitis virus pseudotypes bearing HTLV-1 envelope proteins. We found that the cellular susceptibility to HTLV-1 infection did not correlate with the expression of GLUT1, HS, or NRP-1 alone. To investigate whether other cellular factors were responsible for HTLV-1 susceptibility, we conducted expression cloning. We identified two HS proteoglycan core proteins, syndecan 1 and syndecan 2, as molecules responsible for susceptibility to HTLV-1. We found that treatment of syndecan 1-transduced cells (expressing increased HS) with heparinase, a heparin-degradative enzyme, reduced HTLV-1 susceptibility without affecting the expression levels of HS chains. To further elucidate these results, we characterized the expression of HS chains in terms of the mass, number, and length of HS in several syndecan 1-transduced cell clones as well as human cell lines. We found a significant correlation between HTLV-1 susceptibility and the number of HS chains with short chain lengths. Our findings suggest that a combination of the number and the length of HS chains containing heparin-like regions is a critical factor which affects the cell tropism of HTLV-1.
journal_name
J Viroljournal_title
Journal of virologyauthors
Tanaka A,Jinno-Oue A,Shimizu N,Hoque A,Mori T,Islam S,Nakatani Y,Shinagawa M,Hoshino Hdoi
10.1128/JVI.05783-11subject
Has Abstractpub_date
2012-03-01 00:00:00pages
2959-69issue
6eissn
0022-538Xissn
1098-5514pii
JVI.05783-11journal_volume
86pub_type
杂志文章abstract::Mutant recombinant respiratory syncytial viruses (RSV) which cannot express the NS1 and M2-2 proteins, designated rA2DeltaNS1 and rA2DeltaM2-2, respectively, were evaluated as live-attenuated RSV vaccines. The rA2DeltaNS1 virus contains a large deletion that should have the advantageous property of genetic stability d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.19.9317-9321.2000
更新日期:2000-10-01 00:00:00
abstract::Following intranasal administration, the severe acute respiratory syndrome (SARS) coronavirus replicated to high titers in the respiratory tracts of BALB/c mice. Peak replication was seen in the absence of disease on day 1 or 2, depending on the dose administered, and the virus was cleared within a week. Viral antigen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.7.3572-3577.2004
更新日期:2004-04-01 00:00:00
abstract::The synthesis of different viral ribonucleic acid (RNA) species was studied in chick embryo (CE) and mouse L-cell cultures infected with the Herts strain of Newcastle disease virus (NDV(o)) and a mutant isolated from persistently infected L cells (NDV(pi)). In CE cell cultures, both viruses synthesized significant amo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.3.503-509.1972
更新日期:1972-03-01 00:00:00
abstract::Two second-site mutations in Moloney murine leukemia virus envelope surface protein (SU) were previously shown to rescue infection of two different SU mutants, a fusion-defective point mutant and a fusion-defective modified SU that exhibits weak subunit association. We report here that they also rescue infection of a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.23.11881-11885.2001
更新日期:2001-12-01 00:00:00
abstract::The state of intracellular viral DNA in cells infected with channel catfish virus has been studied by the Hirt selective extraction procedure and by restriction endonuclease digestion. The sedimentation properties and restriction patterns of viral DNA in the Hirt supernatant fraction indicate that the majority, if not...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.2.405-412.1983
更新日期:1983-05-01 00:00:00
abstract::We report on the use of spectral map analysis of the inter- and intraclade neutralization data of 14 sera of human immunodeficiency virus type 1 (HIV-1)-infected individuals and 16 primary isolates, representing genetic clades A to H in group M and group O. This multivariate analysis has been used previously to study ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.6235-6243.1996
更新日期:1996-09-01 00:00:00
abstract::Ground squirrel hepatitis virus (GSHV) is a small DNA virus, structurally and antigenically related to the human hepatitis B virus, which occurs naturally among certain wild populations of ground squirrels (P. L. Marion et al., Proc. Natl. Acad. Sci. U.S.A. 77:2941-2945, 1980). Serum from naturally infected animals wa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.1.366-373.1982
更新日期:1982-10-01 00:00:00
abstract::The effects of recombinant gp120 on the proliferative responses and cytokine production by normal peripheral blood mononuclear cells (PBMC) were investigated. gp120 inhibited in a dose-dependent fashion the anti-CD3 monoclonal antibody (MAb)- and concanavalin A-induced proliferative responses. The production of interl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.8.4953-4960.1996
更新日期:1996-08-01 00:00:00
abstract::Biological and genetic variability is a prominent feature of human immunodeficiency virus (HIV) strains, especially in tropism, syncytium formation, and replicative capacity. To determine whether there were variable host cell effects on HIV replication in monocytes, three different strains of low-passage-number monocy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.7792-7803.1996
更新日期:1996-11-01 00:00:00
abstract::We have cloned several prototypic members of the family of human endogenous retroviruslike elements having a histidine tRNA primer-binding site (RTVL-H) and have determined the nucleotide sequence of one of these clones (RTVL-H2). The RTVL-H2 sequence is 5,813 nucleotides long, with long terminal repeats of 450 nucleo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.12.4060-4066.1987
更新日期:1987-12-01 00:00:00
abstract::A new subgroup of avian leukosis virus (ALV) that includes a unique env gene, designated J, was identified recently in England. Sequence analysis of prototype English isolate HPRS-103 revealed several other unique genetic characteristics of this strain and provided information that it arose by recombination between ex...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.12.10301-10304.1998
更新日期:1998-12-01 00:00:00
abstract::The bacteriophage vB_YecM-ϕR1-37 (ϕR1-37) is a lytic yersiniophage that can propagate naturally in different Yersinia species carrying the correct lipopolysaccharide receptor. This large-tailed phage has deoxyuridine (dU) instead of thymidine in its DNA. In this study, we determined the genomic sequence of phage ϕR1-3...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01783-12
更新日期:2012-12-01 00:00:00
abstract::Open reading frame 24 (ORF24) of murine gammaherpesvirus 68 (MHV-68) is conserved among beta- and gammaherpesviruses; however, its function in viral replication has not been defined. Using MHV-68 as a model, we have identified ORF24 as being essential for viral replication. An ORF24-null virus was generated and shown ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02726-06
更新日期:2007-06-01 00:00:00
abstract::The objective of this investigation was to examine by electron microscopy the replicative ribonucleoprotein (RNP) structures synthesized in vesicular stomatitis virus-infected HeLa cells. Pulse-labeled in vivo products of vesicular stomatitis virus replication and transcription can be separated by centrifugation in Re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.34.3.764-771.1980
更新日期:1980-06-01 00:00:00
abstract::The replication of porcine endogenous retrovirus subgroup A (PERV-A) and PERV-B in certain human cell lines indicates that PERV may pose an infectious risk in clinical xenotransplantation. We have previously reported that human-tropic PERVs isolated from infected human cells following cocultivation with miniature swin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.5.2494-2501.2004
更新日期:2004-03-01 00:00:00
abstract::Late (L) domains containing the highly conserved sequence PPXY were first described for retroviruses, and later research confirmed their conservation and importance for efficient budding of several negative-stranded RNA viruses. Rabies virus (RV), a member of the Rhabdoviridae family, contains the sequence PPEY (amino...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00889-08
更新日期:2008-10-01 00:00:00
abstract::Animals that naturally acquire the prion diseases have a well-developed olfactory sense that they utilize for a variety of basic behaviors. To assess the potential for the nasal cavity to serve as a point of entry for prion diseases, a small amount of prion-infected brain homogenate was placed inferior to the nostrils...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02649-06
更新日期:2007-05-01 00:00:00
abstract:UNLABELLED:CD4+ T cells play a pivotal role in the control of chronic viral infections. Recently, nontraditional CD4+ T cell functions beyond helper effects have been described, and a role for cytolytic CD4+ T cells in the control of HIV infection has been suggested. We define here the transcriptional, phenotypic, and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00438-15
更新日期:2015-08-01 00:00:00
abstract::Borna disease virus (BDV) is a nonsegmented negative-strand (NNS) RNA virus that is unusual because it replicates in the nucleus. The most abundant viral protein in infected cells is a 38/39-kDa doublet that is presumed to represent the nucleocapsid. Infectious particles also contain high levels of this protein, accou...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.7.5133-5139.1997
更新日期:1997-07-01 00:00:00
abstract::N6-adenosine methylation (m6A) is the most common posttranscriptional RNA modification in mammalian cells. We found that most transcripts encoded by the Kaposi's sarcoma-associated herpesvirus (KSHV) genome undergo m6A modification. The levels of m6A-modified mRNAs increased substantially upon stimulation for lytic re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00466-17
更新日期:2017-07-27 00:00:00
abstract::Meq is the major Marek's disease virus (MDV)-encoded oncoprotein and is essential for T-cell lymphomagenesis. Meq and several noncoding RNAs, including three microRNA (MiR) clusters, are expressed from the repeats of the MDV genome during latent infection of T cells. To investigate the state of the chromatin in this a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06113-11
更新日期:2012-02-01 00:00:00
abstract::The lymphoid cell line #1670 has been derived from the infiltrated spleen of a tumor-bearing marmoset monkey infected with Herpesvirus saimiri. The cells contain both types of H. saimiri DNA, unique light (L-) DNA (36% cytosine plus guanine) and repetitive heavy (H-) DNA (71% cytosine plus guanine), without producing ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.22.3.794-803.1977
更新日期:1977-06-01 00:00:00
abstract::Stress signaling from mitochondria and the endoplasmic reticulum (ER) leads to the induction of the proapoptotic transcription factor CHOP/GADD153. Many viruses use the ER as a site of replication and/or envelopment, and this activity can lead to the activation of ER stress and apoptosis. African swine fever virus (AS...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.19.10825-10828.2004
更新日期:2004-10-01 00:00:00
abstract::SERINC3 (serine incorporator 3) and SERINC5 are recently identified host cell inhibitors of HIV-1 particle infectivity that are counteracted by the viral pathogenesis factor Nef. Here we confirm that HIV-1 Nef, but not HIV-1 Vpu, antagonizes the particle infectivity restriction of SERINC5. SERINC5 antagonism occurred ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01246-16
更新日期:2016-11-14 00:00:00
abstract::A unique feature of the large hepadnavirus envelope protein (L) is its mixed transmembrane topology, resulting from partial posttranslational translocation of the pre-S domain. Using protease protection analysis, we demonstrate for duck hepatitis B virus an essential role for the small envelope protein (S) in this pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.5.2455-2458.2000
更新日期:2000-03-01 00:00:00
abstract::CtBP has been shown to be a highly conserved corepressor of transcription. E1A and all the various transcription factors to which CtBP binds contain a conserved PLDLS CtBP-interacting domain, and EBNA3C includes a PLDLS motif (amino acids [aa] 728 to 732). Here we show that EBNA3C binds to CtBP both in vitro and in vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.16.7749-7755.2001
更新日期:2001-08-01 00:00:00
abstract::We have demonstrated that a liposomal array of well-ordered trimers enhances B cell activation, germinal center formation, and the elicitation of tier-2 autologous neutralizing antibodies. Previously, we coupled well-ordered cleavage-independent NFL trimers via their C-terminal polyhistidine tails to nickel lipids int...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00443-17
更新日期:2017-07-27 00:00:00
abstract::The entire genome of the reference strain of bluetongue virus (BTV) serotype 16 (strain RSArrrr/16) was sequenced (a total of 23,518 base pairs). The virus was obtained from the Orbivirus Reference Collection (ORC) at IAH, Pirbright, United Kingdom. The virus strain, which was previously provided by the Onderstepoort ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01672-12
更新日期:2012-09-01 00:00:00
abstract::GS-8374 is a potent HIV protease inhibitor (PI) with a unique diethyl-phosphonate moiety. Due to a balanced contribution of enthalpic and entropic components to its interaction with the protease (PR) active site, the compound retains activity against HIV mutants with high-level multi-PI resistance. We report here the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01211-12
更新日期:2013-01-01 00:00:00
abstract:UNLABELLED:The DNA damage response (DDR) that evolved to repair host cell DNA damage also recognizes viral DNA entering the nucleus during infections. Here, we investigated the modulation of DDR signaling during de novo infection of primary endothelial cells by Kaposi's sarcoma-associated herpesvirus (KSHV). Phosphoryl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03126-13
更新日期:2014-03-01 00:00:00