Abstract:
:Biological and genetic variability is a prominent feature of human immunodeficiency virus (HIV) strains, especially in tropism, syncytium formation, and replicative capacity. To determine whether there were variable host cell effects on HIV replication in monocytes, three different strains of low-passage-number monocytotropic blood isolates of HIV and the laboratory-adapted strain Ba-L were inoculated into panels of adherent monocytes drawn from 44 different donors, and peak extracellular HIV p24 antigen titers were compared. The clinical HIV strains showed patterns of either moderate or low-level replication in most donor monocytes (20 to 4,000 pg/ml). However, within this range there was marked variation in peak titers in most donors. HIV type 1 Ba-L replicated in all donor monocytes to much higher levels with less variability (30 to 40 ng/ml). Furthermore, replication of 21 clinical blood-derived strains of HIV in blood monocytes and monocyte-derived macrophages (MDM) from pairs of identical twins and age-matched unrelated donors (URD) of the same sex were compared. In all of the seven pairs of identical twins, the kinetics of replication (measured by extracellular HIV p24 antigen) of panels of four clinical HIV type 1 isolates in monocytes were similar within pairs. However, marked and significant differences in kinetics of HIV production occurred within 10 of the 12 unrelated donor pairs (P = 0.0007). The remaining two URD pairs showed similar kinetic patterns, but only one pair had the same HLA-DR genotype. Similar results were observed with monocytes/MDMs obtained from a second bleed of the same donor. Hence, discordant patterns of HIV replication kinetics between URD monocyte pairs contrasted with concordant patterns in identical twin monocytes. These data strongly suggest a host cell genetic effect on productive viral replication in monocytes and MDMs. So far, no consistent genetic linkage of HIV replication pattern with HLA-DR genotype has been observed.
journal_name
J Viroljournal_title
Journal of virologyauthors
Chang J,Naif HM,Li S,Sullivan JS,Randle CM,Cunningham ALdoi
10.1128/JVI.70.11.7792-7803.1996subject
Has Abstractpub_date
1996-11-01 00:00:00pages
7792-803issue
11eissn
0022-538Xissn
1098-5514journal_volume
70pub_type
杂志文章abstract::Human cytomegalovirus (HCMV) encodes several proteins that inhibit major histocompatibility complex (MHC) class I-dependent antigen presentation. The HCMV products US2 and US11 are each sufficient for causing the dislocation of human and murine MHC class I heavy chains from the lumen of the endoplasmic reticulum to th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.10.5043-5050.2002
更新日期:2002-05-01 00:00:00
abstract::A study of virus-specific protein synthesis in infectious pancreatic necrosis virus-infected RTG-2 cells was undertaken to find a relationship between the coding capacity of virus genome (two segments of double-stranded RNA of 2.5 X10(6) and 2.3 X 10(6) molecular weight) and the sizes and relative amounts of polypepti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.1.242-258.1977
更新日期:1977-01-01 00:00:00
abstract::Capsid (core) assembly is essential for hepatitis B virus (HBV) replication. We hypothesize that assembly kinetics and stability are tuned for optimal viral replication, not maximal assembly. Assembly effectors (AEfs) are small molecules proposed to disrupt this balance by inappropriately enhancing core assembly. Guid...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03014-12
更新日期:2013-03-01 00:00:00
abstract::The extracellular virion form (EV) of vaccinia virus (VACV) is essential for viral pathogenesis and is difficult to neutralize with antibodies. Why this is the case and how the smallpox vaccine overcomes this challenge remain incompletely understood. We previously showed that high concentrations of anti-B5 antibodies ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02152-12
更新日期:2013-02-01 00:00:00
abstract::Simian immunodeficiency virus (SIV) infection of rhesus macaques has become an important surrogate model for evaluating HIV vaccine strategies. The extreme resistance to neutralizing antibody (NAb) of many commonly used strains, such as SIVmac251/239 and SIVsmE543-3, limits their potential relevance for evaluating the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00923-12
更新日期:2012-08-01 00:00:00
abstract::The long terminal repeat U3 sequences were determined for multiple feline leukemia virus proviruses isolated from naturally occurring T-cell tumors. Heterogeneity was evident, even among proviruses cloned from individual tumors. Proviruses with one, two, or three repeats of the long terminal repeat enhancer sequences ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.4.1675-1682.1990
更新日期:1990-04-01 00:00:00
abstract::The phenotypic properties of ICP27 temperature-sensitive and deletion mutants and the results of transient expression assays have demonstrated that ICP27 has a modulatory effect on viral gene expression induced by ICPs 0 and 4. In order to identify the regions of the ICP27 molecule that are responsible for its enhanci...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.7.3471-3485.1990
更新日期:1990-07-01 00:00:00
abstract::Human cytomegalovirus (HCMV) has been implicated as a potential cofactor in human immunodeficiency virus type 1 (HIV-1)-related disease. Previously, we reported that HCMV inhibits HIV-1 RNA and protein synthesis in cells productively infected with both viruses but, in transient assays, activates an HIV-1 long terminal...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.3.1645-1651.1995
更新日期:1995-03-01 00:00:00
abstract::ICP27 is a multifunctional protein that is required for herpes simplex virus 1 mRNA export. ICP27 interacts with the mRNA export receptor TAP/NXF1 and binds RNA through an RGG box motif. Unlike other RGG box proteins, ICP27 does not bind G-quartet structures but instead binds GC-rich sequences that are flexible in str...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00509-10
更新日期:2010-07-01 00:00:00
abstract::The replication of many positive-strand RNA viruses [(+)RNA viruses] depends on the cellular protein GBF1, but its role in the replication process is not clear. In uninfected cells, GBF1 activates small GTPases of the Arf family and coordinates multiple steps of membrane metabolism, including functioning of the cellul...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00856-19
更新日期:2019-10-15 00:00:00
abstract::Herpes B virus (BV) naturally infects macaque monkeys and is genetically similar to herpes simplex virus (HSV). Zoonotic infection of humans can cause encephalitis and if untreated has a fatality rate of ∼80%. The frequent use of macaques in biomedical research emphasizes the need to understand the molecular basis of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00505-11
更新日期:2011-07-01 00:00:00
abstract::Viral transcription and replication of Ebola virus (EBOV) is balanced by transcription factor VP30, an RNA binding protein. An RNA hairpin at the transcription start site (TSS) of the first gene (NP hairpin) in the 3'-leader promoter is thought to mediate the VP30 dependency of transcription. Here, we investigated the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02215-20
更新日期:2020-12-02 00:00:00
abstract::Herpes simplex virus (HSV) glycoprotein B (gB) is an integral part of the multicomponent fusion system required for virus entry and cell-cell fusion. Here we investigated the mechanism of viral neutralization by the monoclonal antibody (MAb) 2c, which specifically recognizes the gB of HSV type 1 (HSV-1) and HSV-2. Bin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01924-10
更新日期:2011-02-01 00:00:00
abstract::We examined the population structure and genetic variation of four genomic regions within and between 30 Citrus tristeza virus (CTV) isolates from Spain and California. Our analyses showed that most isolates contained a population of sequence variants, with one being predominant. Four isolates showed two major sequenc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.75.17.8054-8062.2001
更新日期:2001-09-01 00:00:00
abstract::We examined the migratory properties of the mouse serum protein responsible for specific neutralization of the endogenous mouse xenotropic type C virus and demonstrated that this neutralizing factor can be transferred from mouse lipoproteins to human and to nonreactive mouse lipoproteins. After acceptance of this acti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.2.365-371.1982
更新日期:1982-05-01 00:00:00
abstract:UNLABELLED:The cellular response to virus infection is initiated when pathogen recognition receptors (PRR) engage viral pathogen-associated molecular patterns (PAMPs). This process results in induction of downstream signaling pathways that activate the transcription factor interferon regulatory factor 3 (IRF3). IRF3 pl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02202-15
更新日期:2015-12-16 00:00:00
abstract::Beta interferon (IFN-beta) expression is triggered by double-stranded RNA, a common intermediate in the replication of many viruses including hepatitis C virus (HCV). The recent development of cell culture-grown HCV allowed us to analyze the IFN signaling pathway following virus infection. In this study, we have exami...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01695-07
更新日期:2007-11-01 00:00:00
abstract::The innate and adaptive immune responses have evolved distinct strategies for controlling different viral pathogens. Encephalomyocarditis virus (EMCV) is a picornavirus that can cause paralysis, diabetes, and myocarditis within days of infection. The optimal innate immune response against EMCV in vivo requires CD1d. I...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02745-05
更新日期:2006-07-01 00:00:00
abstract::We have amplified by the polymerase chain reaction, cloned, and sequenced genomic segments of 118 human papillomavirus type 16 (HPV-16) isolates from 76 cervical biopsy, 14 cervical smear, 3 vulval biopsy, 2 penile biopsy, 2 anal biopsy, and 1 vaginal biopsy sample and two cell lines. The specimens were taken from pat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.4.2057-2066.1992
更新日期:1992-04-01 00:00:00
abstract::The papillomavirus E2 protein is a critical viral regulatory protein with transcription, DNA replication, and genome maintenance functions. We have previously identified the cellular bromodomain protein Brd4 as a major E2-interacting protein and established that it participates in tethering bovine papillomavirus type ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00447-07
更新日期:2007-09-01 00:00:00
abstract::The ability to manipulate the genomes of herpesviruses is of eminent importance for obtaining insight into gene function and regulation of gene expression of these complex viruses. Here we report the use of in vivo overlap recombination to generate pseudorabies virus mutants. Cotransfection of up to five overlapping c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.6.2191-2195.1988
更新日期:1988-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.59.1.120-126.1986
更新日期:1986-07-01 00:00:00
abstract::Through their hemagglutinin-neuraminidase glycoprotein, parainfluenza viruses bind to sialic acid-containing glycoconjugates to initiate infection. Although the virus-receptor interaction is a key factor of infection, the exact nature of the receptors that human parainfluenza viruses recognize has not been determined....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.10.4604-4613.2001
更新日期:2001-05-01 00:00:00
abstract::Among the members of the paramyxovirus family, the transcription process and the components involved have been studied under in vitro conditions thus far. Here, we reexamined the function of the viral RNA-dependent RNA polymerase through infection studies with Sendai virus (SeV) N and P deletion (Delta) mutants. To el...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00914-07
更新日期:2007-12-01 00:00:00
abstract:UNLABELLED:To date, the response of teleost B cells to specific pathogens has been only scarcely addressed. In this work, we have demonstrated that viral hemorrhagic septicemia virus (VHSV), a fish rhabdovirus, has the capacity to infect rainbow trout spleen IgM-positive (IgM(+)) cells, although the infection is not pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03080-14
更新日期:2015-02-01 00:00:00
abstract::Titers of vaccinia virus consistently increased in cultures of washed phytohemagglutinin-treated, peripheral blood leukocytes of a vaccinated adult. Concomitantly, a gradual rise occurred in the numbers of infected leukocytes, as determined by the infective center assay. Increase in viral titer was accompanied by cell...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.8.787-792.1968
更新日期:1968-08-01 00:00:00
abstract::The inhibition of protein synthesis by cycloheximide markedly stimulated adenovirus 2 early transcription (assayed in vivo and in vitro in nuclei) when the drug was added 3 h after infection of HeLa cells. The stimulation was not uniform but ranged from three- to eightfold for region 1A to 10- to 30-fold for region 2 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.2.683-692.1983
更新日期:1983-02-01 00:00:00
abstract::YY1 is a zinc finger transcription factor which acts as either a repressor or an activator dependent on the promoter context. YY1 is a potent activator of the genuine human papillomavirus type 18 (HPV-18) upstream regulatory region (URR) in HeLa cells, which are known for high-level expression of the HPV-18 early gene...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.1.1-12.1995
更新日期:1995-01-01 00:00:00
abstract::Reovirus encephalitis in mice was used as a model system to investigate astrocyte activation (astrogliosis) following viral infection of the brain. Reovirus infection resulted in astrogliosis, as evidenced by increased expression of glial fibrillary acidic protein (GFAP), and the upregulation of genes that have been p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02027-18
更新日期:2019-05-01 00:00:00
abstract::By means of both electron microscopy and plaque assay techniques, 10(-3)m Zn(2+) has been shown to reduce the adsorption rate of male-specific filamentous deoxyribonucleic acid bacteriophages to the tips of F pili. In contrast, 10(-3)m Zn(2+) did not affect the adsorption of male-specific ribonucleic acid phages to th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.4.869-871.1972
更新日期:1972-10-01 00:00:00