Regeneration of herpesviruses from molecularly cloned subgenomic fragments.


:The ability to manipulate the genomes of herpesviruses is of eminent importance for obtaining insight into gene function and regulation of gene expression of these complex viruses. Here we report the use of in vivo overlap recombination to generate pseudorabies virus mutants. Cotransfection of up to five overlapping cloned subgenomic fragments, which together constitute the entire genomic information of pseudorabies virus, results in the efficient reconstitution of virus. This allows the efficient introduction of multiple well-defined mutations in herpesvirus genomes in a single step, without any selection or screening for a particular phenotype.


J Virol


Journal of virology


van Zijl M,Quint W,Briaire J,de Rover T,Gielkens A,Berns A




Has Abstract


1988-06-01 00:00:00












  • Heat Shock Protein 70 Family Members Interact with Crimean-Congo Hemorrhagic Fever Virus and Hazara Virus Nucleocapsid Proteins and Perform a Functional Role in the Nairovirus Replication Cycle.

    abstract:UNLABELLED:The Nairovirus genus of the Bunyaviridae family contains serious human and animal pathogens classified within multiple serogroups and species. Of these serogroups, the Crimean-Congo hemorrhagic fever virus (CCHFV) serogroup comprises sole members CCHFV and Hazara virus (HAZV). CCHFV is an emerging zoonotic v...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Surtees R,Dowall SD,Shaw A,Armstrong S,Hewson R,Carroll MW,Mankouri J,Edwards TA,Hiscox JA,Barr JN

    更新日期:2016-09-29 00:00:00

  • Altered substrate specificity of drug-resistant human immunodeficiency virus type 1 protease.

    abstract::Resistance to human immunodeficiency virus type 1 protease (HIV PR) inhibitors results primarily from the selection of multiple mutations in the protease region. Because many of these mutations are selected for the ability to decrease inhibitor binding in the active site, they also affect substrate binding and potenti...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Dauber DS,Ziermann R,Parkin N,Maly DJ,Mahrus S,Harris JL,Ellman JA,Petropoulos C,Craik CS

    更新日期:2002-02-01 00:00:00

  • Sites of copy choice replication involved in generation of vesicular stomatitis virus defective-interfering particle RNAs.

    abstract::The copy choice model for the generation of defective interfering (DI) particles of vesicular stomatitis virus suggests that during replication the polymerase prematurely terminates, moves with the nascent daughter strand to another site on the same or a different template molecule, and resumes elongation of the nasce...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Meier E,Harmison GG,Keene JD,Schubert M

    更新日期:1984-08-01 00:00:00

  • Targeting Innate Immunity for Antiviral Therapy through Small Molecule Agonists of the RLR Pathway.

    abstract:UNLABELLED:The cellular response to virus infection is initiated when pathogen recognition receptors (PRR) engage viral pathogen-associated molecular patterns (PAMPs). This process results in induction of downstream signaling pathways that activate the transcription factor interferon regulatory factor 3 (IRF3). IRF3 pl...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Pattabhi S,Wilkins CR,Dong R,Knoll ML,Posakony J,Kaiser S,Mire CE,Wang ML,Ireton RC,Geisbert TW,Bedard KM,Iadonato SP,Loo YM,Gale M Jr

    更新日期:2015-12-16 00:00:00

  • Herpes simplex virus tegument protein VP22 contains an internal VP16 interaction domain and a C-terminal domain that are both required for VP22 assembly into the virus particle.

    abstract::Many steps along the herpesvirus assembly and maturation pathway remain unclear. In particular, the acquisition of the virus tegument is a poorly understood process, and the molecular interactions involved in tegument assembly have not yet been defined. Previously we have shown that the two major herpes simplex virus ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Hafezi W,Bernard E,Cook R,Elliott G

    更新日期:2005-10-01 00:00:00

  • Functional Upregulation of the DNA Cytosine Deaminase APOBEC3B by Polyomaviruses.

    abstract:UNLABELLED:The APOBEC3 family of DNA cytosine deaminases has important roles in innate immunity and cancer. It is unclear how DNA tumor viruses regulate these enzymes and how these interactions, in turn, impact the integrity of both the viral and cellular genomes. Polyomavirus (PyVs) are small DNA pathogens that contai...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Verhalen B,Starrett GJ,Harris RS,Jiang M

    更新日期:2016-06-24 00:00:00

  • HeLa nucleic acid contamination in the cancer genome atlas leads to the misidentification of human papillomavirus 18.

    abstract:UNLABELLED:We searched The Cancer Genome Atlas (TCGA) database for viruses by comparing non-human reads present in transcriptome sequencing (RNA-Seq) and whole-exome sequencing (WXS) data to viral sequence databases. Human papillomavirus 18 (HPV18) is an etiologic agent of cervical cancer, and as expected, we found rob...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Cantalupo PG,Katz JP,Pipas JM

    更新日期:2015-04-01 00:00:00

  • Genome homology of vesicular stomatitis virus and defective T particles and evidence for the sequential transcription of the virion ribonucleic acid.

    abstract::The small defective T particle of vesicular stomatitis virus (VSV-111) has no detectable enzyme activity when assayed at 31 C, although qualitatively it possesses all the virion proteins found in complete VSV-1 virions. With VSV-1 transcription product ribonucleic acid (RNA), it is shown that the VSV-111 RNA is identi...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Roy P,Bishop DH

    更新日期:1972-06-01 00:00:00

  • Sindbis virus with a tricomponent genome.

    abstract::We established a system for propagation of Sindbis virus (SIN)-based replicons in tissue culture in the form of a tricomponent genome virus. Three RNA fragments containing complementing genetic information required for virus replication are packaged into separate viral particles, and each cell produces at least 1,000 ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Fayzulin R,Gorchakov R,Petrakova O,Volkova E,Frolov I

    更新日期:2005-01-01 00:00:00

  • Macrophages infected with cytopathic bovine viral diarrhea virus release a factor(s) capable of priming uninfected macrophages for activation-induced apoptosis.

    abstract::Bovine bone marrow-derived macrophages infected with the cytopathic biotype of bovine viral diarrhea virus released an antiviral activity into the supernatant which was tentatively characterized as type I interferon because of its physicochemical properties. Such supernatants primed both infected and uninfected macrop...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Adler B,Adler H,Pfister H,Jungi TW,Peterhans E

    更新日期:1997-04-01 00:00:00

  • Corneal cell survival in adenovirus type 19 infection requires phosphoinositide 3-kinase/Akt activation.

    abstract::Adenovirus type 19 is a major cause of epidemic keratoconjunctivitis, the only ocular adenoviral infection associated with prolonged corneal inflammation. In this study, we investigated the role of phosphoinositide 3-kinase (PI3K) and Akt and their downstream targets in adenovirus infection, and here we report the nov...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Rajala MS,Rajala RV,Astley RA,Butt AL,Chodosh J

    更新日期:2005-10-01 00:00:00

  • Polyprotein-Driven Formation of Two Interdependent Sets of Complexes Supporting Hepatitis C Virus Genome Replication.

    abstract:UNLABELLED:Hepatitis C virus (HCV) requires proteins from the NS3-NS5B polyprotein to create a replicase unit for replication of its genome. The replicase proteins form membranous compartments in cells to facilitate replication, but little is known about their functional organization within these structures. We recentl...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Gomes RG,Isken O,Tautz N,McLauchlan J,McCormick CJ

    更新日期:2015-12-30 00:00:00

  • Knockout of cGAS and STING Rescues Virus Infection of Plasmid DNA-Transfected Cells.

    abstract::It is well known that plasmid DNA transfection, prior to virus infection, negatively affects infection efficiency. Here, we show that cytosolic plasmid DNA activates the cGAS/STING signaling pathway, which ultimately leads to the induction of an antiviral state of the cells. Using a transient one-plasmid clustered reg...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Langereis MA,Rabouw HH,Holwerda M,Visser LJ,van Kuppeveld FJ

    更新日期:2015-11-01 00:00:00

  • Production of high-titer recombinant adeno-associated virus vectors in the absence of helper adenovirus.

    abstract::Recently, efficient and long-term in vivo gene transfer by recombinant adeno-associated virus type 2 (rAAV) vectors has been demonstrated in a variety of tissues. Further improvement in vector titer and purity will expedite this in vivo exploration and provide preclinical information required for use in human gene the...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Xiao X,Li J,Samulski RJ

    更新日期:1998-03-01 00:00:00

  • Green fluorescent protein-tagged retroviral envelope protein for analysis of virus-cell interactions.

    abstract::Fluorescent retroviral envelope (Env) proteins were developed for direct visualization of viral particles. By fusing the enhanced green fluorescent protein (eGFP) to the N terminus of the amphotropic 4070A envelope protein, extracellular presentation of eGFP was achieved. Viruses incorporated the modified Env protein ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Spitzer D,Dittmar KE,Rohde M,Hauser H,Wirth D

    更新日期:2003-05-01 00:00:00

  • Linked tumor-selective virus replication and transgene expression from E3-containing oncolytic adenoviruses.

    abstract::Historically, the adenoviral E3 region was found to be nonessential for viral replication in vitro. In addition, adenoviruses whose genome was more than approximately 105% the size of the native genome were inefficiently packaged. These profound observations were used experimentally to insert transgenes into the adeno...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Zhu M,Bristol JA,Xie Y,Mina M,Ji H,Forry-Schaudies S,Ennist DL

    更新日期:2005-05-01 00:00:00

  • Critical role of Epstein-Barr Virus (EBV)-encoded RNA in efficient EBV-induced B-lymphocyte growth transformation.

    abstract::It was demonstrated that Epstein-Barr virus (EBV)-encoded small RNAs (EBERs) were nonessential for B-lymphocyte growth transformation. We revisited this issue by producing a large quantity of EBER-deleted EBV by using an Akata cell system. Although the EBER-deleted virus efficiently infected B lymphocytes, its 50% tra...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Yajima M,Kanda T,Takada K

    更新日期:2005-04-01 00:00:00

  • Acquisition of sequences homologous to host deoxyribonucleic acid by closed circular simian virus 40 deoxyribonucleic acid.

    abstract::The synthesis of closed circular simian virus 40 (SV40) deoxyribonucleic acid (DNA) containing sequences homologous to host cell DNA depends upon the conditions under which the cells are infected. When BS-C-1 monkey cells were infected with non-plaque-purified virus at low multiplicity of infection [MOI, 0.032 plaque-...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Lavi S,Winocour E

    更新日期:1972-02-01 00:00:00

  • The minimum replication origin of merkel cell polyomavirus has a unique large T-antigen loading architecture and requires small T-antigen expression for optimal replication.

    abstract::Merkel cell polyomavirus (MCV) is a recently discovered human polyomavirus causing the majority of human Merkel cell carcinomas. We mapped a 71-bp minimal MCV replication core origin sufficient for initiating eukaryotic DNA replication in the presence of wild-type MCV large T protein (LT). The origin includes a poly(T...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Kwun HJ,Guastafierro A,Shuda M,Meinke G,Bohm A,Moore PS,Chang Y

    更新日期:2009-12-01 00:00:00

  • Purification, identification, and biochemical characterization of a host-encoded cysteine protease that cleaves a leishmaniavirus gag-pol polyprotein.

    abstract::Leishmania RNA virus (LRV) is a double-stranded RNA virus that infects some strains of the protozoan parasite leishmania As with other totiviruses, LRV presumably expresses its polymerase by a ribosomal frameshift, resulting in a capsid-polymerase fusion protein. We have demonstrated previously that an LRV capsid-poly...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Carrion R Jr,Ro YT,Patterson JL

    更新日期:2003-10-01 00:00:00

  • A single regulatory region modulates both cis activation and trans activation of the herpes simplex virus VP5 promoter in transient-expression assays in vivo.

    abstract::A detailed analysis of the expression of the bacterial chloramphenicol acetyltransferase gene controlled by the herpes simplex virus major capsid protein (VP5) promoter showed that this promoter can be functionally separated into an 80-base core region, which has the minimal information required to serve as a pol II p...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Blair ED,Wagner EK

    更新日期:1986-11-01 00:00:00

  • Extracellular vaccinia virus envelope glycoprotein encoded by the A33R gene.

    abstract::With the aid of three monoclonal antibodies (MAbs), a glycoprotein specifically localized to the outer envelope of vaccinia virus was shown to be encoded by the A33R gene. These MAbs reacted with a glycosylated protein that migrated as 23- to 28-kDa and 55-kDa species under reducing and nonreducing conditions, respect...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Roper RL,Payne LG,Moss B

    更新日期:1996-06-01 00:00:00

  • Repositioning basic residues in the M domain of the Rous sarcoma virus gag protein.

    abstract::The first 86 residues of the Rous sarcoma virus (RSV) Gag protein form a membrane-binding (M) domain that directs Gag to the plasma membrane during budding. Unlike other retroviral Gag proteins, RSV Gag is not myristylated; however, the RSV M domain does contain 11 basic residues that could potentially interact with a...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Callahan EM,Wills JW

    更新日期:2000-12-01 00:00:00

  • Monoclonal antibodies to a monkeypox virus polypeptide determinant.

    abstract::Three monkeypox virus (MPV) antibody-secreting murine monoclones were characterized as being of the immunoglobulin G1 isotype, gave a 4+ reaction in the indirect fluorescent-antibody test, gave a positive reaction in the enzyme immunoassay, and did not neutralize MPV. These monoclonal antibodies were determined by the...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Roumillat LF,Patton JL,Davis ML

    更新日期:1984-10-01 00:00:00

  • Human T-cell leukemia virus type 2 antisense viral protein 2 is dispensable for in vitro immortalization but functions to repress early virus replication in vivo.

    abstract::Human T-cell leukemia virus type 1 (HTLV-1) and HTLV-2 are closely related but pathogenically distinct human retroviruses. The antisense strand of the HTLV-1 genome encodes HTLV-1 basic leucine zipper (b-ZIP) protein (HBZ), a protein that inhibits Tax-mediated viral transcription, enhances T-cell proliferation, and pr...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Yin H,Kannian P,Dissinger N,Haines R,Niewiesk S,Green PL

    更新日期:2012-08-01 00:00:00

  • Binding of transmembrane mucins to galectin-3 limits herpesvirus 1 infection of human corneal keratinocytes.

    abstract::Epithelial cells lining mucosal surfaces impose multiple barriers to viral infection. At the ocular surface, the carbohydrate-binding protein galectin-3 maintains barrier function by cross-linking transmembrane mucins on the apical glycocalyx. Despite these defense mechanisms, many viruses have evolved to exploit fund...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Woodward AM,Mauris J,Argüeso P

    更新日期:2013-05-01 00:00:00

  • Equine infectious anemia virus and the ubiquitin-proteasome system.

    abstract::Some retroviruses contain monoubiquitinated Gag and do not bud efficiently from cells treated with proteasome inhibitors, suggesting an interaction between the ubiquitin-proteasome system and retrovirus assembly. We examined equine infectious anemia virus (EIAV) particles and found that approximately 2% of the p9(Gag)...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Ott DE,Coren LV,Sowder RC 2nd,Adams J,Nagashima K,Schubert U

    更新日期:2002-03-01 00:00:00

  • Dominant-negative inhibition of prion formation diminished by deletion mutagenesis of the prion protein.

    abstract::Polymorphic basic residues near the C terminus of the prion protein (PrP) in humans and sheep appear to protect against prion disease. In heterozygotes, inhibition of prion formation appears to be dominant negative and has been simulated in cultured cells persistently infected with scrapie prions. The results of nucle...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Zulianello L,Kaneko K,Scott M,Erpel S,Han D,Cohen FE,Prusiner SB

    更新日期:2000-05-01 00:00:00

  • Two specific topoisomerase II inhibitors prevent replication of human cytomegalovirus DNA: an implied role in replication of the viral genome.

    abstract::In this study, we show that human cytomegalovirus DNA synthesis is inhibited in infected confluent human embryonic lung cells treated with the DNA-intercalative topoisomerase II inhibitor 4-9'-(acridinylamino)methanesulfon-m-anisidide (m-AMSA). Similar inhibitory effects were observed with VM-26, a nonintercalative to...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Benson JD,Huang ES

    更新日期:1988-12-01 00:00:00

  • Induction of nephroblastoma by myeloblastosis-associated virus type 1: state of proviral DNAs in tumor cells.

    abstract::Myeloblastosis-associated virus type 1 (MAV1) derived from a molecular clone of infectious proviral DNA (B. Perbal, J. S. Lipsick, J. Svoboda, R. F. Silva, and M. A. Baluda, J. Virol. 56:240-244, 1985) was shown to specifically induce nephroblastoma in chickens and therefore belongs to the MAV-N class. We show that ne...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Soret J,Dambrine G,Perbal B

    更新日期:1989-04-01 00:00:00