Abstract:
:The ability to manipulate the genomes of herpesviruses is of eminent importance for obtaining insight into gene function and regulation of gene expression of these complex viruses. Here we report the use of in vivo overlap recombination to generate pseudorabies virus mutants. Cotransfection of up to five overlapping cloned subgenomic fragments, which together constitute the entire genomic information of pseudorabies virus, results in the efficient reconstitution of virus. This allows the efficient introduction of multiple well-defined mutations in herpesvirus genomes in a single step, without any selection or screening for a particular phenotype.
journal_name
J Viroljournal_title
Journal of virologyauthors
van Zijl M,Quint W,Briaire J,de Rover T,Gielkens A,Berns Adoi
10.1128/JVI.62.6.2191-2195.1988subject
Has Abstractpub_date
1988-06-01 00:00:00pages
2191-5issue
6eissn
0022-538Xissn
1098-5514journal_volume
62pub_type
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
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