Abstract:
:ZAM is an env-containing member of the gypsy family of retrotransposons that represents a possible retrovirus of invertebrates. In this paper, we traced ZAM mobilization to get information about a potential path a retroelement may take to reach the germ line of its host. In situ hybridization on whole-mount tissues and immunocytochemistry analyses with antibodies raised against ZAM Gag and Env proteins have shown that all components necessary to assemble ZAM viral particles, i.e., ZAM full-length RNAs and Gag and Env polypeptides, are coexpressed in a small set of follicle cells surrounding the oocyte. By electron microscopy, we have shown that ZAM viral particles are indeed detected in this somatic lineage of cells, which they leave and enter the closely apposed oocyte. Our data provide evidence that the vesicular traffic and yolk granules in the process of vitellogenesis play an important role in ZAM transfer to the oocyte. Our data support the possibility that vitellogenin transfer to the oocyte may help a retroelement pass to the germ line with no need of its envelope product.
journal_name
J Viroljournal_title
Journal of virologyauthors
Leblanc P,Desset S,Giorgi F,Taddei AR,Fausto AM,Mazzini M,Dastugue B,Vaury Cdoi
10.1128/jvi.74.22.10658-10669.2000subject
Has Abstractpub_date
2000-11-01 00:00:00pages
10658-69issue
22eissn
0022-538Xissn
1098-5514journal_volume
74pub_type
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2003-06-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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