Abstract:
:A panel of 28 insertion mutants of the human immunodeficiency virus type 1 (HIV-1) Gag precursor (Pr55Gag) was constructed by linker-insertion mutagenesis and expressed in recombinant baculovirus-infected insect cells. One set of 14 mutants carried the normal N-myristylation signal; the other set constituted their non-N-myristylated counterparts. The mutants were characterized with respect to (i) assembly and extracellular release of membrane-enveloped budding Gag particles, (ii) intracellular assembly and nuclear transport of Gag cores, (iii) specific processing of Pr55Gag by HIV-1 protease in vivo, and (iv) binding of Pr55Gag to an HIV-1 genomic RNA probe in Northwestern blotting. Insertions within the region between amino acid residues 209 and 334 in the CA domain appeared to be the most detrimental to Gag particle assembly and release of Gag into the external medium, whereas a narrower window, between residues 209 and 241, was found to be critical for secretion of soluble Pr55Gag. Differences in Pr55Gag processing in vivo and RNA binding in vitro between N-myristylated and non-N-myristylated Gag mutants suggested a major conformational role for the myristylated N terminus of Gag precursor. In coinfection experiments using wild-type Gag- and mutant Gag-expressing recombinants, a transdominant negative effect on Gag particle assembly and release was observed for insertions located in two separate domains, the matrix and nucleocapsid.
journal_name
J Viroljournal_title
Journal of virologyauthors
Chazal N,Carrière C,Gay B,Boulanger Pdoi
10.1128/JVI.68.1.111-122.1994subject
Has Abstractpub_date
1994-01-01 00:00:00pages
111-22issue
1eissn
0022-538Xissn
1098-5514journal_volume
68pub_type
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pub_type: 杂志文章
doi:10.1128/JVI.71.3.2163-2170.1997
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.2.765-767.1986
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.8.4705-4709.1992
更新日期:1992-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2014-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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