Abstract:
:The hepatitis B virus X protein acts as a transcriptional transactivator in vitro. To elucidate possible biological effects of X protein on liver cells in vivo, we generated four lines of transgenic mice carrying the X gene open reading frame under the control of the human alpha-1-antitrypsin regulatory region. The plasmid construct used to introduce the transgene was shown to encode a 16-kDa X protein with transactivating capability. The expression of X protein was detectable in liver tissue of transgenic animals of three of the lines by immunoblot analysis; levels of expression were highest in the first month after birth of the animals. Over 80 animals from the expressing lines were examined histologically. Most transgenic mice, some of which were observed for up to 2 years, remained normal. However, a few transgenic animals developed mild focal hepatitis, nuclear pleomorphism, focal necrosis, and/or nodular hyperplasia in the liver. Increased mitotic activity of hepatocytes also was observed. We conclude that, at the level of expression achieved in these transgenic mice, the hepatitis B virus transcriptional transactivator X protein alone does not appear to mediate the development of serious liver damage or hepatocellular carcinomas.
journal_name
J Viroljournal_title
Journal of virologyauthors
Lee TH,Finegold MJ,Shen RF,DeMayo JL,Woo SL,Butel JSdoi
10.1128/JVI.64.12.5939-5947.1990subject
Has Abstractpub_date
1990-12-01 00:00:00pages
5939-47issue
12eissn
0022-538Xissn
1098-5514journal_volume
64pub_type
杂志文章abstract::Defective interfering (DI) RNAs of Tomato bushy stunt virus (TBSV), a plus-sense RNA virus, comprise four conserved noncontiguous regions (I through IV) derived from the viral genome. Region III, a 70-nucleotide-long sequence corresponding to a genomic segment located 378 nucleotides upstream of the 3' terminus of the...
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00269-14
更新日期:2014-07-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.24.1.99-107.1977
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.10.6810-6815.1996
更新日期:1996-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.2.620-624.1985
更新日期:1985-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.40.3.691-702.1981
更新日期:1981-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00792-16
更新日期:2016-07-27 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01769-06
更新日期:2007-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00250-19
更新日期:2019-07-30 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.5.2029-2035.2002
更新日期:2002-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.3.1047-1050.1979
更新日期:1979-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01498-13
更新日期:2013-10-01 00:00:00
abstract::Natural killer (NK) cells are an important component in the control of influenza virus infection, acting to both clear virus-infected cells and release antiviral cytokines. Engagement of CD16 on NK cells by antibody-coated influenza virus-infected cells results in antibody-dependent cellular cytotoxicity (ADCC). Incre...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02090-18
更新日期:2019-02-19 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2013-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1983-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1975-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章,评审
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更新日期:2015-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.7.5613-5620.1999
更新日期:1999-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8898-8901.1999
更新日期:1999-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00986-19
更新日期:2019-11-13 00:00:00
abstract::The RNA of infectious bursal disease virus was reexamined in a detailed analysis. It could be established that its genome consists of two segments of double-stranded RNA. The RNA is RNase resistant and has a sedimentation coefficient of 14S and a buoyant density of 1.62 g/ml. The purine/pyrimidine ratio is nearly 1; t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.31.3.584-589.1979
更新日期:1979-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.1.354-360.1990
更新日期:1990-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.11.6242-6251.1991
更新日期:1991-11-01 00:00:00
abstract::The high rate of HIV-1 evolution contributes to immune escape, enables the virus to escape drug therapy, and may underlie the difficulty of producing an effective vaccine. Identifying constraints on HIV evolution is therefore of prime importance. To investigate this problem, we examined the relationships between seque...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00702-10
更新日期:2010-12-01 00:00:00
abstract::Sindbis virus nonstructural polyproteins and their cleavage products are believed to be essential components of viral RNA replication and transcription complexes. Although numerous studies have investigated the effect of mutations in nsP1-, nsP2-, and nsP4-coding regions on Sindbis virus-specific RNA synthesis, relati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.5781-5791.1994
更新日期:1994-09-01 00:00:00