Abstract:
:Natural killer (NK) cells are an important component in the control of influenza virus infection, acting to both clear virus-infected cells and release antiviral cytokines. Engagement of CD16 on NK cells by antibody-coated influenza virus-infected cells results in antibody-dependent cellular cytotoxicity (ADCC). Increasing the potency of antibody-mediated NK cell activity could ultimately lead to improved control of influenza virus infection. To understand if NK cells can be functionally enhanced following exposure to influenza virus-infected cells, we cocultured human peripheral blood mononuclear cells (PBMCs) with influenza virus-infected human alveolar epithelial (A549) cells and evaluated the capacity of NK cells to mediate antibody-dependent functions. Preincubation of PBMCs with influenza virus-infected cells markedly enhanced the ability of NK cells to respond to immune complexes containing hemagglutinin (HA) and anti-HA antibodies or transformed allogeneic cells in the presence or absence of a therapeutic monoclonal antibody. Cytokine multiplex, RNA sequencing, supernatant transfer, Transwell, and cytokine-blocking/cytokine supplementation experiments showed that type I interferons released from PBMCs were primarily responsible for the influenza virus-induced enhancement of antibody-mediated NK cell functions. Importantly, the influenza virus-mediated increase in antibody-dependent NK cell functionality was mimicked by the type I interferon agonist poly(I·C). We conclude that the type I interferon secretion induced by influenza virus infection enhances the capacity of NK cells to mediate ADCC and that this pathway could be manipulated to alter the potency of anti-influenza virus therapies and vaccines.IMPORTANCE Protection from severe influenza may be assisted by antibodies that engage NK cells to kill infected cells through ADCC. Studies have primarily focused on antibodies that have ADCC activity, rather than the capacity of NK cells to become activated and mediate ADCC during an influenza virus infection. We found that type I interferon released in response to influenza virus infection primes NK cells to become highly reactive to anti-influenza virus ADCC antibodies. Enhancing the capacity of NK cells to mediate ADCC could assist in controlling influenza virus infections.
journal_name
J Viroljournal_title
Journal of virologyauthors
Jegaskanda S,Vanderven HA,Tan HX,Alcantara S,Wragg KM,Parsons MS,Chung AW,Juno JA,Kent SJdoi
10.1128/JVI.02090-18subject
Has Abstractpub_date
2019-02-19 00:00:00issue
5eissn
0022-538Xissn
1098-5514pii
JVI.02090-18journal_volume
93pub_type
杂志文章abstract::Thirteen point mutations targeting predicted domains conserved in homologous protein kinases were introduced into the UL97 coding region of the human cytomegalovirus. All mutagenized proteins were expressed in cells infected with recombinant vaccinia viruses (rVV). Several mutations drastically reduced ganciclovir (GC...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8898-8901.1999
更新日期:1999-10-01 00:00:00
abstract::The nucleotide composition of the ribonucleic acid of three strains of rabies virus was determined and found to be similar to that of vesicular stomatitis virus. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.8.6.922-924.1971
更新日期:1971-12-01 00:00:00
abstract::Vaccinia virus has a wide host range and infects mammalian cells of many different species. This suggests that the cell surface receptors for vaccinia virus are ubiquitously expressed and highly conserved. Alternatively, different receptors are used for vaccinia virus infection of different cell types. Here we report ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.2.1577-1585.1998
更新日期:1998-02-01 00:00:00
abstract::Two hundred seventeen field isolates of cucumber mosaic cucumovirus (CMV), sampled from 11 natural populations, were typed by RNase protection assay (RPA) using probes from the genomic RNAs of strains in subgroup I and in subgroup II of CMV strains. Most (85%) of the analyzed isolates belonged to subgroup I. For these...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.2.934-940.1997
更新日期:1997-02-01 00:00:00
abstract::The assembly and budding of Sindbis virus, a prototypic member of the alphavirus subgroup in the family Togaviridae, requires a specific interaction between the nucleocapsid core and the membrane-embedded glycoproteins E1 and E2. These glycoproteins are modified posttranslationally by the addition of palmitic acid, an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.5.2546-2551.1993
更新日期:1993-05-01 00:00:00
abstract::The RNAs of replication-defective murine and primate type C transforming viruses were analyzed for the presence of nucleotide sequences homologous to the genomes of their respective helper type C viruses by using DNAs complementary (cDNA) to either the 5'-terminal (cDNA5') or total (cDNAtotal) nucleotide sequences of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.3.570-576.1978
更新日期:1978-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.12.6585-6594.2004
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abstract::A molecular clone of mouse-neuroadapted yellow fever 17D virus (SPYF-MN) was used to identify critical determinants of viral neuroinvasiveness in a SCID mouse model. Virus derived from this clone differs from nonneuroinvasive YF5.2iv virus at 29 nucleotide positions, encoding 13 predicted amino acid substitutions and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.22.12232-12242.2003
更新日期:2003-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.8.6475-6481.1998
更新日期:1998-08-01 00:00:00
abstract::Infection of monkey cells with human adenovirus (Ad) is abortive, but the infection can be enhanced by coinfecting with simian virus 40 (SV40). However, in the coinfected monkey cells, Ad interferes strongly with SV40 DNA biosynthesis. This interference was found to be a reproducible, delicately controlled phenomenon ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.27.2.275-287.1978
更新日期:1978-08-01 00:00:00
abstract::The localization of H-1 viral replicative-form double-stranded DNA and progeny single-stranded DNA replication in parasynchronously infected, simian virus 40-transformed newborn human kidney cells was studied with high-resolution electron microscope autoradiography (80-nm silver grains). We analyzed wild-type H-1 and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.1.349-360.1978
更新日期:1978-01-01 00:00:00
abstract::Complexes of f2 phage RNA and its A protein, or maturation protein, transfect Escherichia coli cells much better than does protein-free RNA. We used these complexes to introduce the bacteriophage f2 lysis gene into cells. The A protein-RNA complex was found to kill cells, probably by causing them to leak large macromo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.1.60-66.1981
更新日期:1981-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.1.22-27.1974
更新日期:1974-01-01 00:00:00
abstract::Numerous deaths of koi and common carp (Cyprinus carpio) were observed on many farms throughout Israel, resulting in severe financial losses. The lethal viral disease observed is highly contagious and extremely virulent, but morbidity and mortality are restricted to koi and common carp populations. Diseased fish exhib...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.4.1983-1991.2005
更新日期:2005-02-01 00:00:00
abstract:UNLABELLED:The increasing prevalence of ranavirus (RV; Iridoviridae) infections of wild and commercially maintained aquatic species is raising considerable concerns. While Xenopus laevis is the leading model for studies of immunity to RV, amphibian antiviral interferon (IFN) responses remain largely uncharacterized. Ac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00223-14
更新日期:2014-05-01 00:00:00
abstract::Mutations critical for the central nervous system (CNS) attenuation of the Sabin vaccine strains of poliovirus (PV) are located within the viral internal ribosome entry site (IRES). We examined the interaction of the IRESs of PV type 3 (PV3) and Sabin type 3 (Sabin3) with polypyrimidine tract-binding protein (PTB) and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.20.11097-11107.2004
更新日期:2004-10-01 00:00:00
abstract::Ribosomes are observed intimately associated with nucleocapsids of vesicular, stomatitis virus, especially those that line structures that are either cytoplasmic vesicles or invaginations of the plasma membrane. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.2.447-452.1975
更新日期:1975-08-01 00:00:00
abstract::To examine the pathway of the coreceptor switching of CCR5-using (R5) virus to CXCR4-using (X4) virus in simian-human immunodeficiency virus SHIV(SF162P3N)-infected rhesus macaque BR24, analysis was performed on variants present at 20 weeks postinfection, the time when the signature gp120 V3 loop sequence of the X4 sw...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00570-08
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.2398-2405.1998
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.5.4316-4326.1999
更新日期:1999-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.10.5922-5931.1993
更新日期:1993-10-01 00:00:00
abstract::Measles virus (MeV) infection is characterized by the formation of multinuclear giant cells (MGC). We report that beta interferon (IFN-beta) production is amplified in vitro by the formation of virus-induced MGC derived from human epithelial cells or mature conventional dendritic cells. Both fusion and IFN-beta respon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00078-07
更新日期:2007-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.3.1486-1494.2000
更新日期:2000-02-01 00:00:00
abstract::Neutralizing antibodies may have critical importance in immunity against human immunodeficiency virus type 1 (HIV-1) infection. However, the amount of protective antibody needed at mucosal surfaces has not been fully established. Here, we evaluated systemic and mucosal pharmacokinetics (PK) and pharmacodynamics (PD) o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01361-13
更新日期:2013-11-01 00:00:00
abstract:UNLABELLED:Cell culture systems reproducing virus replication can serve as unique models for the discovery of novel bioactive molecules. Here, using a hepatitis C virus (HCV) cell culture system, we identified neoechinulin B (NeoB), a fungus-derived compound, as an inhibitor of the liver X receptor (LXR). NeoB was init...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00856-16
更新日期:2016-09-29 00:00:00
abstract::CtBP has been shown to be a highly conserved corepressor of transcription. E1A and all the various transcription factors to which CtBP binds contain a conserved PLDLS CtBP-interacting domain, and EBNA3C includes a PLDLS motif (amino acids [aa] 728 to 732). Here we show that EBNA3C binds to CtBP both in vitro and in vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.16.7749-7755.2001
更新日期:2001-08-01 00:00:00
abstract::A porcine rotavirus (prv), variant 4F, isolated in tissue culture from the feces of a Chinese pig with diarrhea, was found to have become highly pathogenic when passaged in gnotobiotic piglets (J. C. Bridger, B. Burke, G. M. Beards, and U. Desselberger, J. Gen. Virol. 73:3011-3015, 1992). Comparison with the closely r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.853-857.1997
更新日期:1997-01-01 00:00:00
abstract::Growth of bacteriophage T7 is inhibited by the antibiotic coumermycin A1, an inhibitor of the Escherichia coli DNA gyrase. Since growth of the phage is insensitive to the antibiotic in strains containing a coumermycin-resistant DNA gyrase, this enzyme appears to be required for phage growth. We have investigated the e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.2.529-535.1979
更新日期:1979-02-01 00:00:00
abstract::The nonstructural protein (NSs) of severe fever with thrombocytopenia syndrome phlebovirus (SFTSV) sequesters TANK-binding kinase 1 (TBK1) into NSs-induced cytoplasmic structures to inhibit the phosphorylation and nuclear translocation of interferon (IFN) regulatory factor 3 (IRF3) and subsequent interferon beta (IFN-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00706-18
更新日期:2018-09-12 00:00:00
abstract::Dendritic cells (DC) are believed to play an important role in the initiation of innate and adaptive immune responses to infection, including respiratory tract infections, where respiratory DC (RDC) perform this role. In this report, we examined the susceptibilities of isolated murine RDC to influenza virus infection ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02367-07
更新日期:2008-05-01 00:00:00