Abstract:
:Inoculation of the leafhopper cell line AC-20 with wound tumor virus resulted in a productive noncytopathic infection with no detectable alteration of cellular protein synthesis. Virus-specific polypeptide synthesis, detectable by 8 h postinoculation, increased in a linear fashion, reaching a peak (approximately 10 to 15% of total protein synthesis) by 48 h postinoculation. The rate of viral protein synthesis continued at this level for several days but declined, relative to cellular protein synthesis, as infected cells were passaged. By passage 10, the synthesis of viral polypeptides was reduced to a level approximately 5% of that observed at 48 h postinoculation. Viral protein synthesis was not stimulated by superinfection. Viral antigens and infectious virus persisted in the majority (greater than 90%) of cells in an infected culture even after more than 100 passages. The synthesis of wound tumor virus polypeptides in infected insect vector cells appears to be regulated in a coordinated and selective manner.
journal_name
J Viroljournal_title
Journal of virologyauthors
Peterson AJ,Nuss DLdoi
10.1128/JVI.56.2.620-624.1985subject
Has Abstractpub_date
1985-11-01 00:00:00pages
620-4issue
2eissn
0022-538Xissn
1098-5514journal_volume
56pub_type
杂志文章abstract::A retrovirus vector has been developed that selects for instances in which the provirus integrates in close proximity to cellular promoters. Coding sequences for a selectable marker (histidinol dehydrogenase) were inserted into the 3' long terminal repeat (LTR) of an enhancerless Molony murine leukemia virus. Although...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.8.3227-3233.1989
更新日期:1989-08-01 00:00:00
abstract::Cytomegalovirus (CMV) infection is the most common opportunistic infection in immunosuppressed individuals, such as transplant recipients or people living with HIV/AIDS, and congenital CMV is the leading viral cause of developmental disabilities in infants. Due to the highly species-specific nature of CMV, animal mode...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05840-11
更新日期:2011-12-01 00:00:00
abstract::Integrated retroviruses or retroviral vectors may be transcriptionally inactive although their promoter-enhancer regions are able to direct transcription in the host cell. We have used single-gene retroviral vectors with a long terminal repeat-directed neo marker gene to determine if the level of transcription relates...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.5596-5601.1994
更新日期:1994-09-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) impacts multiple lineages of hematopoietic cells, including lymphocytes and macrophages, either by direct infection or indirectly by perturbations of cell networks, leading to generalized immune deficiency. We designed a study to discover, in primary human macrophages, senti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.21.11477-11486.2004
更新日期:2004-11-01 00:00:00
abstract::The bovine papillomavirus E5 gene encodes a 44-amino-acid, homodimeric transmembrane protein that is the smallest known transforming protein. The E5 protein transforms cultured fibroblasts by forming a stable complex with the endogenous platelet-derived growth factor (PDGF) beta receptor through transmembrane and juxt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.3264-3272.1999
更新日期:1999-04-01 00:00:00
abstract::In varicella-zoster virus (VZV)-infected primary human brain vascular adventitial fibroblasts (BRAFs), levels of beta interferon (IFN-β,) STAT1, and STAT2 transcripts as well as STAT1 and STAT2 protein were decreased. IFN-α transcript levels were increased but not secreted IFN-α protein levels. Compared to IFN-α-treat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01945-14
更新日期:2014-10-01 00:00:00
abstract::The mechanisms involved in the emergence of virulent mammalian viruses were investigated in the adult immunodeficient SCID mouse infected by the attenuated prototype strain of the parvovirus Minute Virus of Mice (MVMp). Cloned MVMp intravenously inoculated in mice consistently evolved during weeks of subclinical infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.17.11280-11290.2005
更新日期:2005-09-01 00:00:00
abstract:UNLABELLED:One of the first lines of host defense against many viruses in vertebrates is the innate immune system, which detects pathogen-associated molecular patterns (PAMPs) using pathogen recognition receptors (PRR). The dynamic interactions between pathogens and hosts create, in some cases, species-specific relatio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03576-14
更新日期:2015-03-01 00:00:00
abstract::A vigorous expansion of antigen-specific CD8(+) T cells lacking apparent effector function was observed in a rhesus macaque acutely infected with the simian immunodeficiency virus (SIV) strain SIVmac239. Antigen-specific CD8(+) T cells were identified using antigenic-peptide class I major histocompatibility complex te...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.6.3028-3033.2001
更新日期:2001-03-01 00:00:00
abstract::Herpesvirus lytic DNA replication requires both the cis-acting element, the origin, and trans-acting factors, including virally encoded origin-binding protein, DNA replication enzymes, and auxiliary factors. Two lytic DNA replication origins (ori-Lyt) of Kaposi's sarcoma-associated herpesvirus (KSHV) have been identif...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01319-07
更新日期:2008-03-01 00:00:00
abstract::Measles is a highly contagious human disease caused by the measles virus (MeV). In this study, by proteomic analysis, we identified peroxiredoxin 1 (Prdx1) as a host factor that binds to the C-terminal region of the nucleoprotein (N; N(TAIL)) of MeV. Glutathione S-transferase (GST) pulldown experiments showed that the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01796-10
更新日期:2011-03-01 00:00:00
abstract::Strains of Theiler's murine encephalomyelitis virus (TMEV) are divided into two subgroups, TO and GDVII. TMEV strains show subgroup-specific virus growth and cell tropism and induce subgroup-specific diseases. Using site-directed mutagenesis, we demonstrated that the amino acid at position 57 of the leader protein (L(...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00693-06
更新日期:2006-12-01 00:00:00
abstract::The protein kinase C (PKC) pathway has been considered to be essential for activation of latent Epstein-Barr virus (EBV) into the lytic cycle. The phorbol ester tetradecanoyl phorbol acetate (TPA), a PKC agonist, is one of the best understood activators of EBV lytic replication. Zp, the promoter of the EBV immediate-e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.11.5612-5626.2002
更新日期:2002-06-01 00:00:00
abstract::PLC/PRF/5, a tissue culture cell line isolated from a human hepatocellular carcinoma and producing hepatitis B surface antigen, was studied for the presence of hepatitis B virus (HBV)-specific DNA and RNA. PLC/PRF/5 cell DNA accelerated the rate of reassociation of HBV [32P]DNA, and quantitative experiments indicated ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.33.2.795-806.1980
更新日期:1980-02-01 00:00:00
abstract::Lack of disease in long-term nonprogressors with human immunodeficiency virus type 1 (HIV-1) infection was strongly associated with very low copy numbers of HIV-1 DNA and RNA in peripheral blood mononuclear cells and plasma and the presence of high levels of anti-HIV-1 CD8+ memory cytotoxic T lymphocytes specific for ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.9.5838-5842.1995
更新日期:1995-09-01 00:00:00
abstract::Techniques are described for the growth and rapid purification of the avian coronavirus infectious bronchitis virus (IBV). Purified IBV has a sedimentation coefficient of 320S and a buoyant density of 1.22 g/ml in sucrose-deuterium oxide equilibrium gradients. IBV RNA extracted by proteinase K in the presence of sodiu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.24.1.99-107.1977
更新日期:1977-10-01 00:00:00
abstract::Influenza virus infection initiates transcription of a variety of genes for cytokines such as tumor necrosis factor alpha (TNF-alpha), TNF-beta, interleukin 1 alpha, (IL-1 alpha), IL-1 beta, IL-2, IL-4, IL-6, IL-10, granulocyte macrophage colony-stimulating factor, and gamma interferon. However, the mechanism by which...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.3.1480-1484.1995
更新日期:1995-03-01 00:00:00
abstract::The efficient attachment of pseudorabies virus to cultured cells is dependent on an electrostatic interaction between negatively charged cell surface heparan sulfate and the viral envelope glycoprotein gC. Deletion of the first one-third of gC severely impairs virus attachment, but the mutant virions are still capable...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.834-839.1995
更新日期:1995-02-01 00:00:00
abstract::The cytolytic T-lymphocyte (CTL) response to respiratory infection with equine herpesvirus 1 (EHV-1) in CBA (H-2(k)) mice was investigated. Intranasal (i.n.) inoculation of mice with the attenuated EHV-1 strain KyA resulted in the generation of a primary virus-specific CTL response in the draining mediastinal lymph no...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.7.5366-5372.1998
更新日期:1998-07-01 00:00:00
abstract::The nucleic acids of influenza A, B, and C viruses were compared. Susceptibility to nucleases demonstrates that influenza C virus, just as influenza A and B viruses, possesses single-stranded RNA as its genome. The base compositions of the RNAs of influenza A, B, and influenza C virus are almost identical and comparat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.2.738-744.1976
更新日期:1976-05-01 00:00:00
abstract::Replicase gene expression by the human coronavirus 229E involves the synthesis of two large polyproteins, pp1a and pp1ab. Experimental evidence suggests that these precursor molecules are subject to extensive proteolytic processing. In this study, we show that a chymotrypsin-like enzyme, the virus-encoded 3C-like prot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.1.177-185.1999
更新日期:1999-01-01 00:00:00
abstract::Varicella-zoster virus (VZV) encodes within its unique long region a gene product with protein kinase motifs. In a previous study, we demonstrated that immunoprecipitated VZV open reading frame (ORF) 47 protein was associated with a functional protein kinase activity, on the basis of its ability to both autophosphoryl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.3.1350-1359.1994
更新日期:1994-03-01 00:00:00
abstract::Retroviral integrases associate during the early viral life cycle with preintegration complexes that catalyze the integration of reverse-transcribed viral cDNA into the host chromosomes. Several cellular and viral proteins have been reported to be incorporated in the preintegration complex. This study demonstrates tha...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02681-09
更新日期:2010-08-01 00:00:00
abstract:UNLABELLED:Cavally virus (CavV) and related viruses in the family Mesoniviridae diverged profoundly from other nidovirus lineages but largely retained the characteristic set of replicative enzymes conserved in the Coronaviridae and Roniviridae. The expression of these enzymes in virus-infected cells requires the extens...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02040-14
更新日期:2014-12-01 00:00:00
abstract::The fraction of the adenovirus type 5 genome expressed as mRNA in a rat cell line, 5RK, transformed by the HsuI-G restriction fragment was analyzed by saturation hybridization using separated strands of 32P-labeled HindIII-G fragment. It was found that 45 to 50% of G fragment (about 8 X 10(5) daltons) is expressed as ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.3.1011-1014.1978
更新日期:1978-12-01 00:00:00
abstract::Mouse mammary tumor virus (MMTV)-induced mammary adenocarcinomas can develop from several different premalignant precursors common in GR mice. Insertion mutagenesis of the mammary protooncogenes int-1 and int-2 was studied in this multistep system by analyzing samples from various stages of neoplastic development for ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.4.1794-1802.1990
更新日期:1990-04-01 00:00:00
abstract::Many viral fusion proteins are primed by proteolytic cleavage near their fusion peptides. While the coronavirus (CoV) spike (S) protein is known to be cleaved at the S1/S2 boundary, this cleavage site is not closely linked to a fusion peptide. However, a second cleavage site has been identified in the severe acute res...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00079-09
更新日期:2009-08-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) infection requires cell surface expression of CD4. Costimulation of CD8(+)/CD4(-) T lymphocytes by anti-CD3 and anti-CD28 antibodies or by allogeneic dendritic cells induced expression of CD4 and rendered these CD8 cells susceptible to HIV-1 infection. Naive CD45RA+ cells re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.9054-9060.1998
更新日期:1998-11-01 00:00:00
abstract::The fusion (F) protein of simian virus 5 (strain W3A) induced extensive cell fusion in BHK cells when expressed alone, while that of strain WR did not. Mutational analysis demonstrated that the fusing activity can be transferred to the WR F protein by a proline residue at position 22 of subunit W3A F2. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.12.9855-9858.1997
更新日期:1997-12-01 00:00:00
abstract::The synthesis of minus-strand RNA was studied in cell cultures infected with the heat-resistant strain of Sindbis virus and with temperature-sensitive (ts) belonging to complementation groups A, B, F, and G, all of which exhibited an RNA-negative (RNA-) phenotype when infection was initiated and maintained at 39 degre...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.2.348-358.1981
更新日期:1981-08-01 00:00:00