Abstract:
:Measles is a highly contagious human disease caused by the measles virus (MeV). In this study, by proteomic analysis, we identified peroxiredoxin 1 (Prdx1) as a host factor that binds to the C-terminal region of the nucleoprotein (N; N(TAIL)) of MeV. Glutathione S-transferase (GST) pulldown experiments showed that the Prdx1-binding site overlapped with the MeV phosphoprotein (P)-binding site on N(TAIL) and that Prdx1 competed for the binding to N(TAIL) with the P protein, which is a component of RNA-dependent RNA polymerase (RdRp). Furthermore, RNA interference for Prdx1 resulted in a significant reduction in MeV growth in HEK293-SLAM cells. A minigenome assay indicated that Prdx1 suppression affected the viral RNA transcription and/or replication step. Relative quantification of viral RNA by real-time PCR (RT-PCR) showed that Prdx1 suppression not only reduced viral RNA transcription and replication but also enhanced polar attenuation in viral mRNA transcription. Surface plasmon resonance analysis showed that the binding affinity of Prdx1 to MeV-N was 40-fold lower than that of MeV-P to MeV-N, which suggested that Prdx1 might be involved in the early stage of MeV infection, when the expression level of Prdx1 was much higher than that of MeV-P. Since Prdx1 was expressed abundantly and constitutively in various cells, the results in this study indicate that Prdx1 is one of the inherent host factors implicated in MeV RNA synthesis.
journal_name
J Viroljournal_title
Journal of virologyauthors
Watanabe A,Yoneda M,Ikeda F,Sugai A,Sato H,Kai Cdoi
10.1128/JVI.01796-10subject
Has Abstractpub_date
2011-03-01 00:00:00pages
2247-53issue
5eissn
0022-538Xissn
1098-5514pii
JVI.01796-10journal_volume
85pub_type
杂志文章abstract::The membrane glycoproteins Gn and Gc of Hantaan virus (HTNV) (family Bunyaviridae) are modified by N-linked glycosylation. The glycoproteins contain six potential sites for the attachment of N-linked oligosaccharides, five sites on Gn and one on Gc. The properties of the N-linked oligosaccharide chains were analyzed b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.10.5414-5422.2004
更新日期:2004-05-01 00:00:00
abstract::Targeted vectors will be necessary for many gene therapy applications. To target retroviruses to melanomas, we fused a single-chain variable fragment antibody (scFv) directed against the surface glycoprotein high-molecular-weight melanoma-associated antigen (HMW-MAA) to the amphotropic murine leukemia virus envelope. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.8.6923-6929.1999
更新日期:1999-08-01 00:00:00
abstract::JC virus (JCV) is a DNA virus causing progressive multifocal leukoencephalopathy (PML) in immunodeficient patients. In the present study, 22 genetic quasispecies with more than 1.5% variant frequency were detected in JCV genomes from six clinical samples of PML by next-generation sequencing. A mutation from A to C at ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01335-16
更新日期:2016-12-16 00:00:00
abstract:UNLABELLED:Kaposi's sarcoma-associated herpesvirus (KSHV) is the causative agent for Kaposi sarcoma (KS), primary effusion lymphoma (PEL), and a subset of multicentric Castleman disease (MCD). The KSHV life cycle has two principal gene repertoires, latent and lytic. KSHV viral interleukin-6 (vIL-6), an analog of human ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01192-15
更新日期:2015-10-21 00:00:00
abstract::Adeno-associated virus (AAV) vectors are being considered for in vivo applications of gene therapy in the treatment of a variety of disorders. This study evaluates the biology of second-generation vectors based on the novel serotypes AAV7 and AAV8 and containing self-complementary genomes in the nonhuman primate liver...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00526-06
更新日期:2006-06-01 00:00:00
abstract::Simian virus 40 (SV40) infection of human diploid cells failed to cause an enhanced production of thymidine kinase during the first 10 days after infection. Thymidine kinase activities from extracts of SV40-transformed cultures (human or simian) were considerably higher than the activity levels in extracts from the no...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.1.5.912-919.1967
更新日期:1967-10-01 00:00:00
abstract::The K15 gene of Kaposi's sarcoma-associated herpesvirus (also known as human herpesvirus 8) consists of eight alternatively spliced exons and has been predicted to encode membrane proteins with a variable number of transmembrane regions and a common C-terminal cytoplasmic domain with putative binding sites for SH2 and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.17.9346-9358.2003
更新日期:2003-09-01 00:00:00
abstract::Signal transducers and activators of transcription (STATs) relay signals from activated cell surface receptors directly to the nucleus. Previously, a protein required for T-cell transformation by the DNA tumor virus herpesvirus saimiri (HVS) and designated tyrosine kinase interacting protein (Tip-484) was shown to int...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.9.6677-6682.1997
更新日期:1997-09-01 00:00:00
abstract::Feline leukemia viruses (FeLVs) belonging to interference subgroup C induce fatal anemia resembling human pure red cell aplasia (PRCA). Subgroup A FeLVs, although closely related genetically to FeLVs of subgroup C, do not induce PRCA. The determinants for PRCA induction by a molecularly cloned prototype subgroup C vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.9.5561-5568.1992
更新日期:1992-09-01 00:00:00
abstract::Actinophage MSP2 is infectious for Streptomyces venezuelae S13. Based upon electron microscopy of coliphage T4 mixed with MSP2, MSP2 had a head about 48 +/- 2 nm wide and 87 +/- 5 nm long. DNA from polyoma virus and from coliphages T4 and T7 served as reference markers in estimating the molecular weight of MSP2 DNA fr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.3.736-740.1975
更新日期:1975-09-01 00:00:00
abstract::Rotavirus NSP1 has been shown to function as an E3 ubiquitin ligase that mediates proteasome-dependent degradation of interferon (IFN) regulatory factors (IRF), including IRF3, -5, and -7, and suppresses the cellular type I IFN response. However, the effect of rotavirus NSP1 on viral replication is not well defined. P...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00585-09
更新日期:2009-07-01 00:00:00
abstract::There is controversy as to whether the cell entry mechanism of Sindbis virus (SIN) involves direct fusion of the viral envelope with the plasma membrane at neutral pH or uptake by receptor-mediated endocytosis and subsequent low-pH-induced fusion from within acidic endosomes. Here, we studied the membrane fusion activ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8476-8484.1999
更新日期:1999-10-01 00:00:00
abstract::The major adenovirus (Ad) capsid proteins hexon, penton, and fiber influence the efficiency and tropism of gene transduction by Ad vectors. Fiber is the high-affinity receptor binding protein that serves to mediate cell attachment in vitro when using coxsackie-adenovirus receptor (CAR)-containing cell lines. This cont...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.18.11627-11637.2005
更新日期:2005-09-01 00:00:00
abstract::The roles of intracellular naked (INV), cell-associated enveloped (CEV), and extracellular enveloped (EEV) forms of vaccinia virus in cell-to-cell and longer-range spread were investigated by using two closely related strains of vaccinia virus, WR and IHD-J. We confirmed previous results that WR and IHD-J produced sim...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.7.4170-4179.1992
更新日期:1992-07-01 00:00:00
abstract::Muscle is an attractive target for gene delivery because of its mass and because vectors can be delivered in a noninvasive fashion. Adeno-associated virus (AAV) has been shown to be effective for muscle-targeted gene transfer. Recent progress in characterization of AAV serotype 1 (AAV1) and AAV6 demonstrated that thes...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.4.2768-2774.2003
更新日期:2003-02-01 00:00:00
abstract::In vitro transcription of the avian tumor virus RNA by RNA-directed DNA polymerase is initiated on the unique cellular 4S RNA. Previous studies have shown that on the average there is one such RNA primer hydrogen bonded to each viral 35S RNA. The present study confirms that finding and demonstrates that, at least for ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.3.553-558.1975
更新日期:1975-09-01 00:00:00
abstract::Hepatitis A virus (HAV) has previously been reported to bind to human red blood cells through interaction with glycophorin A. Residue K221 of VP1 and the surrounding VP3 residues are involved in such an interaction. This capsid region is specifically recognized by the monoclonal antibody H7C27. A monoclonal antibody-r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00707-12
更新日期:2012-08-01 00:00:00
abstract::Latency-associated transcript (LAT) promoter deletion mutants of herpes simplex virus type 1 have a reduced capacity to reactivate following adrenergic induction in the rabbit eye model. We have mapped a reactivation phenotype within LAT and describe the construction of recombinants in which poly(A) addition sites hav...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.4.2449-2459.1996
更新日期:1996-04-01 00:00:00
abstract::Coronavirus subgenomic mRNA possesses a 5'-end leader sequence which is derived from the 5' end of genomic RNA and is linked to the mRNA body sequence. This study examined whether coronavirus transcription involves a discontinuous transcription step; the possibility that a leader sequence from mouse hepatitis virus (M...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.4.2615-2623.1994
更新日期:1994-04-01 00:00:00
abstract::The infected cell polypeptide 4 (ICP4) of herpes simplex virus 1 (HSV-1) is a regulator of viral transcription that is required for productive infection. Since viral genes are transcribed by cellular RNA polymerase II (RNA pol II), ICP4 must interact with components of the pol II machinery to regulate viral gene expre...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00385-11
更新日期:2011-06-01 00:00:00
abstract::A mutation that disrupts the interaction between the NS2 protein of minute virus of mice and the nuclear export factor Crm1 results in a block to egress of mutant-generated full virions from the nucleus of infected murine cells. These mutants produce wild-type levels of monomer and dimer replicative DNA forms but are ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.7.3257-3266.2002
更新日期:2002-04-01 00:00:00
abstract::The flavivirus dengue virus (DV) infects cells through a low-pH-triggered membrane fusion reaction mediated by the viral envelope protein E. E is an elongated transmembrane protein with three domains and is organized as a homodimer on the mature virus particle. During fusion, the E protein homodimer dissociates, inser...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00170-10
更新日期:2010-06-01 00:00:00
abstract::Vaccinia virus (VACV) stimulates long-term immunity against highly pathogenic orthopoxvirus infection of humans (smallpox) and mice (mousepox [ectromelia virus {ECTV}]) despite the lack of a natural host-pathogen relationship with either of these species. Previous research revealed that VACV is able to induce polyfunc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00038-12
更新日期:2012-07-01 00:00:00
abstract::The human papillomavirus type 18 (HPV-18) upstream regulatory region (URR) controls viral gene transcription in a cell-type-specific manner. The HPV-18 URR is active in HeLa cells but inactive in HepG2 cells. The activating activity of YY1 in HeLa cells is dependent on its functional interactions with the switch regio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.7695-7705.1996
更新日期:1996-11-01 00:00:00
abstract::Hydroxyurea, an inhibitor of ribonucleotide reductase, blocks replication of vaccinia virus. However, when medium containing hydroxyurea and dialyzed serum was supplemented with deoxyadenosine, the block to viral reproduction was circumvented, provided that an inhibitor of adenosine deaminase was also present. Deoxygu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.5.2290-2298.1991
更新日期:1991-05-01 00:00:00
abstract::Cell extracts from murine A9 or human HeLa cells containing wild-type copies the NS1 polypeptide of minute virus of mice (MVM), produced from a recombinant vaccinia virus, can support the resolution of viral 3' termini from palindromic junction fragments of dimeric, replicative-form MVM DNA. Resolution resulted in the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.3.1579-1589.1993
更新日期:1993-03-01 00:00:00
abstract::The 5'-terminal nucleotide sequence from positions 50 to 130 of vesicular stomatitis virus RNA was determined indirectly by using a defective interfering particle RNA which contains covalently linked genomic minus and antigenomic plus sense RNAs. The last 18 nucleotides of the L gene coding for in the viral polymerase...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.34.2.550-559.1980
更新日期:1980-05-01 00:00:00
abstract::A phage, designated CP-51, that carries out generalized transduction in Bacillus cereus 569 was isolated from soil. All auxotrophic mutants tested, those requiring tryptophan, histidine, leucine, isoleucine, methionine, or phenylalanine, were transduced to prototrophy. The phage was extremely unstable when stored at 2...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.7.657-662.1968
更新日期:1968-07-01 00:00:00
abstract::Neurons of the sensory ganglia are the major site of varicella-zoster virus (VZV) latency and may undergo productive infection during reactivation. Although the VZV glycoprotein E/glycoprotein I (gE/gI) complex is known to be critical for neurovirulence, few studies have assessed the roles of these proteins during inf...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02293-13
更新日期:2013-12-01 00:00:00
abstract::The mechanism of cellular src (c-src) transduction by a transformation-defective deletion mutant, td109, of Rous sarcoma virus was studied by sequence analysis of the recombinational junctions in three td109-derived recovered sarcoma viruses (rASVs). Our results show that two rASVs have been generated by recombination...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.59.3.556-563.1986
更新日期:1986-09-01 00:00:00