Abstract:
:The human papillomavirus type 18 (HPV-18) upstream regulatory region (URR) controls viral gene transcription in a cell-type-specific manner. The HPV-18 URR is active in HeLa cells but inactive in HepG2 cells. The activating activity of YY1 in HeLa cells is dependent on its functional interactions with the switch region which is critical for the HPV-18 URR activity in HeLa cells. Here, we show that a protein complex composed of C/EBP beta and YY1 binds the switch region which is detected only in HeLa cells, not in HepG2 cells. Transfection of C/EBP beta into HepG2 cells restored the formation of the C/EBP beta-YY1-switch region complex, accompanied by increased transcription directed by the HPV-18 URR. Mutations in the switch region that abolished the complex formation also abrogated C/EBP beta-induced transcriptional activation. This provides a strong correlation between the binding of the C/EBP beta-YY1 complex to the switch region and cell-type-specific URR activity. Taken together, we have identified a novel C/EBP beta-YY1 complex that binds the switch region and contributes to cell-type-specific HPV-18 URR activity.
journal_name
J Viroljournal_title
Journal of virologyauthors
Bauknecht T,See RH,Shi Ydoi
10.1128/JVI.70.11.7695-7705.1996subject
Has Abstractpub_date
1996-11-01 00:00:00pages
7695-705issue
11eissn
0022-538Xissn
1098-5514journal_volume
70pub_type
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journal_title:Journal of virology
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doi:10.1128/JVI.44.3.877-881.1982
更新日期:1982-12-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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doi:10.1128/jvi.74.5.2203-2209.2000
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.5.603-611.1971
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.2.364-373.1985
更新日期:1985-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.00662-12
更新日期:2012-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.2650-2657.1999
更新日期:1999-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.12.7271-7275.1993
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journal_title:Journal of virology
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更新日期:1986-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2017-08-10 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.5.2149-2156.1990
更新日期:1990-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2003-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.43.1.92-103.1982
更新日期:1982-07-01 00:00:00
abstract::MicroRNAs (miRNAs) have emerged as key regulators in many biological processes, from development to defense, at almost all organismal levels. Recently, their role has been highlighted in pathogen-host interactions. Emerging evidence from a variety of virus-host systems indicates that cellular as well as virally encode...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01794-09
更新日期:2010-01-01 00:00:00
abstract::Open reading frame P (ORF P), located at the 3' terminus of the 8.5-kb DNA sequence transcribed during latency and almost completely antisense to the gamma(1)34.5 gene, is naturally repressed by infected cell protein 4 (ICP4), the major herpes simplex virus 1 regulatory protein. Earlier studies on cells infected with ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.10.7750-7757.1997
更新日期:1997-10-01 00:00:00