Evidence for coronavirus discontinuous transcription.

abstract：:Viral interference studies have demonstrated the existence of four distinct murine leukemia virus (MuLV) receptors on NIH 3T3 mouse cells. The four viral interference groups are ecotropic MuLV; mink cell focus inducing virus (MCF); amphotropic MuLV; and 10A1, a recombinant derivative of amphotropic MuLV that uses a un...

journal_title：Journal of virology

authors： Ott D,Friedrich R,Rein A

更新日期：1990-02-01 00:00:00

abstract：:Virions produced by cells in the presence of actinomycin D (Act D virions) contain reverse transcriptase but are deficient in 70S genomic RNA. To assess the role of genomic RNA in encapsidation of a functional reverse transcriptase and to study the interaction of the enzyme and its template in the cores of intact viri...

journal_title：Journal of virology

authors： Gerwin BI,Levin JG

更新日期：1977-11-01 00:00:00

abstract：:Growth of phage phi105 and its deoxyribonucleic acid (DNA) was studied in radiation-sensitive mutants of Bacillus subtilis. The recA gene is required for optimal prophage induction with mitomycin C and for infectivity of prophage DNA. rec B gene is required for marker rescue from mature DNA. The importance of bacteria...

journal_title：Journal of virology

authors： Rutberg B,Rutberg L

更新日期：1971-12-01 00:00:00

abstract：:The U(S)3 open reading frame of herpes simplex virus 1 (HSV-1) was reported to encode two mRNAs each directing the synthesis of the same protein. We report that the U(S)3 gene encodes two proteins. The predominant U(S)3 protein is made in wild-type HSV-1-infected cells. The truncated mRNA and a truncated protein desig...

journal_title：Journal of virology

authors： Poon AP,Roizman B

更新日期：2005-07-01 00:00:00

abstract：:It has been suggested that sequences located within the 5' noncoding region of human foamy virus (HFV) are critical for expression of the viral Gag and Pol structural proteins. Here, we identify a discrete approximately 151-nucleotide sequence, located within the R region of the HFV long terminal repeat, that activate...

journal_title：Journal of virology

authors： Russell RA,Zeng Y,Erlwein O,Cullen BR,McClure MO

更新日期：2001-08-01 00:00:00

abstract：:Coronaviruses generally have a narrow host range, infecting one or just a few species. Using targeted RNA recombination, we constructed a mutant of the coronavirus mouse hepatitis virus (MHV) in which the ectodomain of the spike glycoprotein (S) was replaced with the highly divergent ectodomain of the S protein of fel...

journal_title：Journal of virology

authors： Kuo L,Godeke GJ,Raamsman MJ,Masters PS,Rottier PJ

更新日期：2000-02-01 00:00:00

abstract：:We found that recircularized high-risk (type 16 and 18) and low-risk mucosal (type 6b and 11) and cutaneous (type 5 and 8) human papillomavirus (HPV) genomes replicate readily when delivered into U2OS cells by electroporation. The replication efficiency is dependent on the amount of input HPV DNA and can be followed f...

journal_title：Journal of virology

authors： Geimanen J,Isok-Paas H,Pipitch R,Salk K,Laos T,Orav M,Reinson T,Ustav M Jr,Ustav M,Ustav E

更新日期：2011-04-01 00:00:00

abstract：:For the identification of RNA-binding proteins that specifically interact with potato spindle tuber viroid (PSTVd), we subjected a tomato cDNA expression library prepared from viroid-infected leaves to an RNA ligand screening procedure. We repeatedly identified cDNA clones that expressed a protein of 602 amino acids. ...

journal_title：Journal of virology

authors： Martínez de Alba AE,Sägesser R,Tabler M,Tsagris M

更新日期：2003-09-01 00:00:00

abstract：:Murine cytomegalovirus propagated in mouse embryo fibroblasts was purified by the following procedures. (i) Extracellular virus was concentrated by centrifugation at 100,000 x g for 90 min. (ii) The concentrated virus was passed through a Bio-Rad Bio-Gel A-15m column to eliminate contaminating materials smaller than 1...

journal_title：Journal of virology

authors： Kim KS,Sapienza VJ,Carp RI,Moon HM

更新日期：1976-03-01 00:00:00

abstract：:Neutralizing antibodies may have critical importance in immunity against human immunodeficiency virus type 1 (HIV-1) infection. However, the amount of protective antibody needed at mucosal surfaces has not been fully established. Here, we evaluated systemic and mucosal pharmacokinetics (PK) and pharmacodynamics (PD) o...

journal_title：Journal of virology

authors： Klein K,Veazey RS,Warrier R,Hraber P,Doyle-Meyers LA,Buffa V,Liao HX,Haynes BF,Shaw GM,Shattock RJ

更新日期：2013-11-01 00:00:00

abstract：:The human T-cell leukemia virus type I (HTLV-I) envelope protein is synthesized as a gp61 precursor product cleaved into two mature proteins, a gp45 exterior protein and a gp20 anchoring the envelope at the cell membrane. Using N-glycosylation inhibitors and site-directed mutagenesis of the potential glycosylation sit...

journal_title：Journal of virology

authors： Pique C,Pham D,Tursz T,Dokhélar MC

更新日期：1992-02-01 00:00:00

abstract：:Cells of Mus minutoides, an African pygmy mouse of the subgenus Nannomys, are susceptible to ecotropic Moloney and Friend mouse leukemia viruses (MLVs) but not to AKV-type MLVs. Transfected MA139 ferret cells expressing the mCAT-1 cell surface receptor, with the minCAT-1 substitutions K222Q and V233L, did not restrict...

journal_title：Journal of virology

authors： Yan Y,Kozak CA

更新日期：2008-07-01 00:00:00

abstract：:Autophagy is an intracellular degradation pathway that provides a host defense mechanism against intracellular pathogens. However, many viruses exploit this mechanism to promote their replication. This study shows that lytic induction of Epstein-Barr virus (EBV) increases the membrane-bound form of LC3 (LC3-II) and LC...

journal_title：Journal of virology

authors： Hung CH,Chen LW,Wang WH,Chang PJ,Chiu YF,Hung CC,Lin YJ,Liou JY,Tsai WJ,Hung CL,Liu ST

更新日期：2014-10-01 00:00:00

abstract：:Here, we report that specific manipulations of the cellular response to virus infection can cause prevention of apoptosis and consequent establishment of persistent infection. Infection of several human cell lines with Sendai virus (SeV) or human parainfluenza virus 3, two prototypic paramyxoviruses, caused slow apopt...

journal_title：Journal of virology

authors： Peters K,Chattopadhyay S,Sen GC

更新日期：2008-04-01 00:00:00

abstract：:Vaccinia virus has a wide host range and infects mammalian cells of many different species. This suggests that the cell surface receptors for vaccinia virus are ubiquitously expressed and highly conserved. Alternatively, different receptors are used for vaccinia virus infection of different cell types. Here we report ...

journal_title：Journal of virology

authors： Chung CS,Hsiao JC,Chang YS,Chang W

更新日期：1998-02-01 00:00:00

abstract：UNLABELLED:Of the various genetic subtypes of human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2) and simian immunodeficiency virus (SIV), only in subtype C of HIV-1 is a genetically variant NF-κB binding site found at the core of the viral promoter in association with a subtype-specific Sp1III motif. How the ...

journal_title：Journal of virology

authors： Verma A,Rajagopalan P,Lotke R,Varghese R,Selvam D,Kundu TK,Ranga U

更新日期：2016-07-27 00:00:00

abstract：:Three polyadenylated RNAs, 9, 7.3, and 3.5 kilobases long, of a human endogenous retrovirus, ERV3, are abundant in human placental chorion, representing about 0.03 to 0.05% of the total mRNA. We characterized the structure of these mRNAs by Northern blot and S1 nuclease mapping analyses. We found that all three RNAs w...

journal_title：Journal of virology

authors： Kato N,Pfeifer-Ohlsson S,Kato M,Larsson E,Rydnert J,Ohlsson R,Cohen M

更新日期：1987-07-01 00:00:00

abstract：UNLABELLED:Respiratory syncytial virus (RSV), a member of the Paramyxoviridae family of nonsegmented, negative-sense, single-stranded RNA genome viruses, is a leading cause of lower respiratory tract infections in infants, young children, and the elderly or immunocompromised. There are many open questions regarding the...

journal_title：Journal of virology

authors： Kiss G,Holl JM,Williams GM,Alonas E,Vanover D,Lifland AW,Gudheti M,Guerrero-Ferreira RC,Nair V,Yi H,Graham BS,Santangelo PJ,Wright ER

更新日期：2014-07-01 00:00:00

abstract：:Primary RNA transcripts from the human immunodeficiency virus type 1 (HIV-1) are processed into mature mRNA by a complex series of splicing events. Viral structural proteins and reverse transcriptase are translated from unspliced or singly spliced transcripts. Proteins which control virus replication, including tat, r...

journal_title：Journal of virology

authors： Robert-Guroff M,Popovic M,Gartner S,Markham P,Gallo RC,Reitz MS

更新日期：1990-07-01 00:00:00

abstract：:We describe the use of herpesvirus promoters to regulate the expression of a Sindbis virus replicon (SINrep/LacZ). We isolated cell lines that contain the cDNA of SINrep/LacZ under the control of a promoter from a herpesvirus early gene which requires regulatory proteins encoded by immediate-early genes for expression...

journal_title：Journal of virology

authors： Ivanova L,Schlesinger S,Olivo PD

更新日期：1999-03-01 00:00:00

abstract：:The effect DNA repair might have on the integration of exogenous proviral DNA into host cell DNA was investigated by comparing the efficiency of proviral DNA integration in normal chicken embryonic fibroblasts and in chicken embryonic fibroblasts treated with UV or 4-nitroquinoline-1-oxide. The cells were treated with...

journal_title：Journal of virology

authors： Tsuruo T,Baluda MA

更新日期：1977-09-01 00:00:00

abstract：:We investigated the effect of HLA class I alleles on clinical parameters for HIV-1 disease progression in the Japanese population, where two strongly protective alleles, HLA-B*57 and HLA-B*27, are virtually nonexistent. HLA-B alleles showed a dominant role, primarily through HLA-B*67:01 and the HLA-B*52:01-C*12:02 hap...

journal_title：Journal of virology

authors： Naruto T,Gatanaga H,Nelson G,Sakai K,Carrington M,Oka S,Takiguchi M

更新日期：2012-10-01 00:00:00

abstract：:Repeated passages of vaccinia virus in increasing concentrations of hydroxyurea followed by plaque purification resulted in the isolation of variants capable of growth in 5 mM hydroxyurea, a drug concentration which inhibited the reproduction of wild-type vaccinia virus 1,000-fold. Analyses of viral protein synthesis ...

journal_title：Journal of virology

authors： Slabaugh MB,Mathews CK

更新日期：1986-11-01 00:00:00

abstract：:Hepatitis B virus (HBV) DNA clones were propagated from 57 carriers with antibody to hepatitis B e antigen (HBeAg) and sequenced within nucleotides (nt) 1685 to 1926 including the core promoter (nt 1742 to 1849) and the pre-C region (nt 1814 to 1900). Mutations in the core promoter or those in the pre-C region, or bot...

journal_title：Journal of virology

authors： Okamoto H,Tsuda F,Akahane Y,Sugai Y,Yoshiba M,Moriyama K,Tanaka T,Miyakawa Y,Mayumi M

更新日期：1994-12-01 00:00:00

abstract：UNLABELLED:In a genome-wide small interfering RNA (siRNA) screen, we recently identified the interferon (IFN)-inducible protein 35 (IFI35; also known as IFP35) as a factor required for vesicular stomatitis virus (VSV) infection. Studies reported here were conducted to further understand the role and requirement of IFI3...

journal_title：Journal of virology

authors： Das A,Dinh PX,Panda D,Pattnaik AK

更新日期：2014-03-01 00:00:00

abstract：:Sequences and structures within the terminal genomic regions of plus-strand RNA viruses are targets for the binding of host proteins that modulate functions such as translation, RNA replication, and encapsidation. Using murine norovirus 1 (MNV-1), we describe the presence of long-range RNA-RNA interactions that were s...

journal_title：Journal of virology

authors： López-Manríquez E,Vashist S,Ureña L,Goodfellow I,Chavez P,Mora-Heredia JE,Cancio-Lonches C,Garrido E,Gutiérrez-Escolano AL

更新日期：2013-11-01 00:00:00

abstract：UNLABELLED:Cattle have been proposed as the natural reservoir of a novel member of the virus family Orthomyxoviridae, which has been tentatively classified as influenza D virus (IDV). Although isolated from sick animals, it is unclear whether IDV causes any clinical disease in cattle. To address this aspect of Koch's p...

journal_title：Journal of virology

authors： Ferguson L,Olivier AK,Genova S,Epperson WB,Smith DR,Schneider L,Barton K,McCuan K,Webby RJ,Wan XF

更新日期：2016-05-27 00:00:00

abstract：:Early in infection by bacteriophage T4, before replication has commenced, one can detect the presence of newly synthesized DNA which cosediments with parental phage DNA on sucrose gradients. As shown earlier (R. E. Murray and C. K. Mathews, 1969), some of this represents covalent attachment of new material to parental...

journal_title：Journal of virology

authors： Scofield MS,Collinsworth WL,Mathews CK

更新日期：1974-04-01 00:00:00

abstract：:The strain of Newcastle disease virus (NDV(pi)) present in persistently infected L cells differed markedly from the Herts strain (NDV(0)) used to initiate the infection. NDV(pi) produced small plaques (less than 1 mm) in chick embryo cell cultures, whereas the wild type (NDV(0)) produced large plaques (2 to 3 mm). The...

journal_title：Journal of virology

authors： Thacore H,Youngner JS

更新日期：1969-09-01 00:00:00

abstract：:Measles virus (MeV) is an enveloped RNA virus bearing two envelope glycoproteins, the hemagglutinin (H) and fusion (F) proteins. Upon receptor binding, the H protein triggers conformational changes of the F protein, causing membrane fusion and subsequent virus entry. MeV may persist in the brain, infecting neurons and...

journal_title：Journal of virology

authors： Shirogane Y,Hashiguchi T,Yanagi Y

更新日期：2020-01-06 00:00:00