Abstract:
:For the identification of RNA-binding proteins that specifically interact with potato spindle tuber viroid (PSTVd), we subjected a tomato cDNA expression library prepared from viroid-infected leaves to an RNA ligand screening procedure. We repeatedly identified cDNA clones that expressed a protein of 602 amino acids. The protein contains a bromodomain and was termed viroid RNA-binding protein 1 (VIRP1). The specificity of interaction of VIRP1 with viroid RNA was studied by different methodologies, which included Northwestern blotting, plaque lift, and electrophoretic mobility shift assays. VIRP1 interacted strongly and specifically with monomeric and oligomeric PSTVd positive-strand RNA transcripts. Other RNAs, for example, U1 RNA, did not bind to VIRP1. Further, we could immunoprecipitate complexes from infected tomato leaves that contained VIRP1 and viroid RNA in vivo. Analysis of the protein sequence revealed that VIRP1 is a member of a newly identified family of transcriptional regulators associated with chromatin remodeling. VIRP1 is the first member of this family of proteins, for which a specific RNA-binding activity is shown. A possible role of VIRP1 in viroid replication and in RNA mediated chromatin remodeling is discussed.
journal_name
J Viroljournal_title
Journal of virologyauthors
Martínez de Alba AE,Sägesser R,Tabler M,Tsagris Mdoi
10.1128/jvi.77.17.9685-9694.2003subject
Has Abstractpub_date
2003-09-01 00:00:00pages
9685-94issue
17eissn
0022-538Xissn
1098-5514journal_volume
77pub_type
杂志文章abstract::The interaction of papillomavirus E2 proteins with cellular Brd4 protein is important for transcriptional regulation of viral genes and partitioning of viral genomes. Bovine papillomavirus type 1 (BPV-1) E2 binds cellular chromatin in complex with Brd4 in both mitotic and interphase cells. To identify specific sites o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02275-08
更新日期:2009-03-01 00:00:00
abstract::A rapid assay for retroviral reverse transcriptase activity released into the culture medium by infected cells was developed. With the assay, 4,000 clonally infected cell lines could be tested in a few hours. We have adapted the assay for use as a screen for the detection of spontaneous viral mutants. Mutants of Molon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.1.239-248.1981
更新日期:1981-04-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) is linked to several neoplastic diseases: Kaposi's sarcoma, primary effusion lymphoma (PEL), and multicentric Castleman's disease (MCD). KSHV replicates actively, via a controlled gene expression program, but can also remain latent. It had been thought that the transition...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06617-11
更新日期:2012-04-01 00:00:00
abstract::We have mapped the temperature-sensitive (ts) lesions of three mutants, ts51, ts53, and ts65, and two other mutants, ts7 and ts20, to regions on the vaccinia virus genome that encode the 147- and 22-kilodalton subunits of the viral DNA-dependent RNA polymerase, respectively. Plasmid and bacteriophage clones from the H...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.2.705-713.1989
更新日期:1989-02-01 00:00:00
abstract:UNLABELLED:Kaposi's sarcoma-associated herpesvirus (KSHV) typically displays two different phases in its life cycle, the default latent phase and the lytic phase. There is a short period of lytic gene expression in the early stage of KSHV primary infection. The factors involved in the shutdown process of lytic gene exp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00596-14
更新日期:2014-07-01 00:00:00
abstract::The vpr sequences from six human immunodeficiency virus type 1 (HIV-1)-infected mother-infant pairs following perinatal transmission were analyzed. We found that 153 of the 166 clones analyzed from uncultured peripheral blood mononuclear cell DNA samples showed a 92.17% frequency of intact vpr open reading frames. The...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.8.6937-6943.1998
更新日期:1998-08-01 00:00:00
abstract::The wild-type strain of vesicular stomatitis virus (VSV) contains in its complete virion (VSV-1, B particles) a minus strand RNA. The principle defective particle of the wild-type strain (VSV-111, T particles) contains a shorter minus strand, homologous to part of the VSV-1 genome. Neither virion contains any detectab...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.11.6.915-925.1973
更新日期:1973-06-01 00:00:00
abstract::Human immunodeficiency virus (HIV) infections in sub-Saharan Africa represent about 56% of global infections. Study of active-site mutations (the V82A single mutation and the V82F I84V double mutation) in the less-studied South African HIV type 1 subtype C (C-SA) protease indicated that neither mutation had a signific...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00726-08
更新日期:2008-11-01 00:00:00
abstract::An immunodominant envelope glycoprotein is encoded by the human herpesvirus 8 (HHV-8) (also termed Kaposi's sarcoma-associated herpesvirus) K8.1 gene. The functional role of glycoprotein K8.1 is unknown, and recognizable sequence homology to K8.1 is not detectable in the genomes of most other closely related gammaherp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.23.11583-11593.2001
更新日期:2001-12-01 00:00:00
abstract::Vaccinia virus (VACV) stimulates long-term immunity against highly pathogenic orthopoxvirus infection of humans (smallpox) and mice (mousepox [ectromelia virus {ECTV}]) despite the lack of a natural host-pathogen relationship with either of these species. Previous research revealed that VACV is able to induce polyfunc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00038-12
更新日期:2012-07-01 00:00:00
abstract::Interaction between viral proteins is necessary for viral replication and viral particle assembly. We used the yeast two-hybrid assay to identify interactions among all the mature proteins of the hepatitis C virus. The interaction between NS3 and NS3 was one of the strongest viral protein-protein interactions detected...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.1.205-214.2001
更新日期:2001-01-01 00:00:00
abstract::Progressive multifocal leukoencephalopathy (PML) is an often-fatal demyelinating disease of the central nervous system. PML results when oligodendrocytes within immunocompromised individuals are infected with the human JC virus (JCV). We have identified an oligodendrocyte precursor cell line, termed G144, that support...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00735-17
更新日期:2017-09-27 00:00:00
abstract::The mechanism of action of hepatitis B virus (HBV) X protein in transcriptional transactivation and in tumorigenesis remains obscure. We have used the yeast two-hybrid system to identify a cellular protein that can interact with HBV X protein. This protein, designated X-associated protein 1 (XAP-1), is a human homolog...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.1107-1114.1995
更新日期:1995-02-01 00:00:00
abstract::Toroviruses (family Coronaviridae, order Nidovirales) are enveloped, positive-stranded RNA viruses that have been implicated in enteric disease in cattle and possibly in humans. Despite their potential veterinary and clinical relevance, little is known about torovirus epidemiology and molecular genetics. Here, we pres...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.17.9567-9577.2003
更新日期:2003-09-01 00:00:00
abstract::Human bocavirus (HBoV) is a newly discovered parvovirus associated with acute respiratory tract illness (ARTI) and gastrointestinal illness. No previous reports indicated the presence of HBoV infection in Jiangsu Province, China. Here we report three complete genomic sequences of HBoV strains from children with gastro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02618-12
更新日期:2012-12-01 00:00:00
abstract::Sonchus yellow net virus is a plant nucleorhabdovirus whose nucleocapsid (N), phosphoprotein (P), and polymerase (L) proteins form large viroplasms in the nuclei of infected plants (C. R. F. Martins, J. A. Johnson, D. M. Lawrence, T. J. Choi, A. Pisi, S. L. Tobin, D. Lapidus, J. D. O. Wagner, S. Ruzin, K. McDonald, an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02349-06
更新日期:2007-05-01 00:00:00
abstract::All human influenza pandemics have originated from avian influenza viruses. Although multiple changes are needed for an avian virus to be able to transmit between humans, binding to human-type receptors is essential. Several research groups have reported mutations in H5N1 viruses that exhibit specificity for human-typ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02016-17
更新日期:2018-04-27 00:00:00
abstract::Lassa fever (LF) is a potentially lethal human disease that is caused by the arenavirus Lassa virus (LASV). Annually, around 300,000 infections with up to 10,000 deaths occur in regions of Lassa fever endemicity in West Africa. Here we demonstrate that mice lacking a functional STAT1 pathway are highly susceptible to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01433-13
更新日期:2013-10-01 00:00:00
abstract::Retroviral Gag proteins have the ability to induce budding and particle release from the plasma membrane when expressed in the absence of all of the other virus-encoded components; however, the locations of the functional domains within the Gag protein that are important for this process are poorly understood. It was ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.9.5550-5561.1993
更新日期:1993-09-01 00:00:00
abstract:UNLABELLED:Marek's disease virus (MDV), an alphaherpesvirus, is the causative agent of a lethal disease in chickens characterized by generalized nerve inflammation and rapid lymphoma development. The extensive colinearity of the MDV genome with those of related herpesviruses has eased functional characterization of man...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02687-14
更新日期:2015-01-15 00:00:00
abstract:UNLABELLED:Arthritogenic alphaviruses such as Ross River virus (RRV) and chikungunya virus (CHIKV) cause large-scale epidemics of severe musculoskeletal disease and have been progressively expanding their global distribution. Since its introduction in July 2014, CHIKV now circulates in the United States. The hallmark o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00224-15
更新日期:2015-08-01 00:00:00
abstract::The inducible transcription factor NF-kappaB is commonly activated upon RNA virus infection and is a key player in the induction and regulation of the innate immune response. Borna disease virus (BDV) is a neurotropic negative-strand RNA virus, which replicates in the nucleus of the infected cell and causes a persiste...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.10.6043-6051.2005
更新日期:2005-05-01 00:00:00
abstract::To analyze the biological activities of the alphavirus glycoproteins, eight different monoclonal antibodies against the two glycoproteins of western equine encephalitis virus were isolated. Five of the eight monoclonal antibodies were shown to be specific for E1 and three for E2 protein by an enzyme-linked immunosorbe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.55.3.840-842.1985
更新日期:1985-09-01 00:00:00
abstract::The nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid virus, Ad2(+)ND(1), differs from the defective Ad-SV40 hybrid populations previously described, in that this hybrid virus can replicate without the aid of nonhybrid adenovirus helper. Consequently, the deoxyribonucleic acid (DNA) from this virus, which ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.3.352-358.1971
更新日期:1971-03-01 00:00:00
abstract::Molecularly cloned proviral DNA of avian oncogenic retrovirus CMII was isolated by screening a genomic library of a CMII-transformed quail cell line with a myc-specific probe. On a 10.4-kilobase EcoRI fragment, the cloned DNA contained 4.4 kilobases of CMII proviral sequences extending from the 5' long terminal repeat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.2.576-585.1985
更新日期:1985-05-01 00:00:00
abstract::The roles of intracellular naked (INV), cell-associated enveloped (CEV), and extracellular enveloped (EEV) forms of vaccinia virus in cell-to-cell and longer-range spread were investigated by using two closely related strains of vaccinia virus, WR and IHD-J. We confirmed previous results that WR and IHD-J produced sim...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.7.4170-4179.1992
更新日期:1992-07-01 00:00:00
abstract::Alphaherpesviruses are parasites of the peripheral nervous system in their natural hosts. After the initial infection of peripheral tissues such as mucosal cells, these neurotropic viruses will invade the peripheral nervous system that innervates the site of infection via long-distance axonal transport of the viral ge...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.17.10875-10889.2005
更新日期:2005-09-01 00:00:00
abstract::HB-3, a temperate bacteriophage of Streptococcus pneumoniae, synthesizes its own murein hydrolase activity when multiplying on cultures of pneumococcus. The enzyme (HBL) was purified and biochemically characterized as an N-acetylmuramoyl-L-alanine amidase of 36,000 daltons, and a 2.1-kilobase DraI fragment containing ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.1.137-142.1990
更新日期:1990-01-01 00:00:00
abstract::By pretreating simian virus 40-infected BSC-1 cells with glucosamine, [(3)H]uridine labeling of both cellular and viral RNA can be halted instantaneously by addition of cold uridine. We have studied the fate of pulse-labeled viral RNA from cells at 45 h postinfection under these conditions. During a 5-min period of la...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.2.590-599.1978
更新日期:1978-11-01 00:00:00
abstract::Epstein-Barr virus (EBV) is typically acquired asymptomatically in childhood. In contrast, infection later in life often leads to infectious mononucleosis (IM), a febrile illness characterized by anti-EBV IgM antibody positivity, high loads of circulating latently infected B cells, and a marked lymphocytosis caused by...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00382-17
更新日期:2017-10-13 00:00:00