Abstract:
:Sonchus yellow net virus is a plant nucleorhabdovirus whose nucleocapsid (N), phosphoprotein (P), and polymerase (L) proteins form large viroplasms in the nuclei of infected plants (C. R. F. Martins, J. A. Johnson, D. M. Lawrence, T. J. Choi, A. Pisi, S. L. Tobin, D. Lapidus, J. D. O. Wagner, S. Ruzin, K. McDonald, and A. O. Jackson, J. Virol. 72:5669-5679, 1998). When expressed alone, the N protein localizes to the nuclei of plant and yeast (Saccharomyces cerevisiae) cells and the P protein is distributed throughout the cells, but coexpression of N and P results in formation of subnuclear viroplasm-like foci (M. M. Goodin, J. Austin, R. Tobias, M. Fujita, C. Morales, and A. O. Jackson, J. Virol. 75:9393-9406, 2001; M. M. Goodin, R. G. Dietzgen, D. Schichnes, S. Ruzin, and A. O. Jackson, Plant J. 31:375-383, 2002). We now show that the N protein and various fluorescent derivatives form similar subnuclear foci in plant cells and that homologous interactions mediated by a helix-loop-helix region near the amino terminus are required for formation of the foci. Mutations within the helix-loop-helix region also interfere with N- and P-protein interactions that are required for N and P colocalization in the subnuclear foci. Affinity purification of N proteins harboring single mutations within the motif revealed that Tyr40 is critical for N-N and N-P interactions. Additional in vitro binding assays also indicated that the N protein binds to yeast and plant importin alpha homologues, whereas mutations in the carboxy-terminal nuclear localization signal abrogate importin alpha binding. The P protein did not bind to the importin alpha homologues, suggesting that the N and P proteins use different pathways for nuclear entry. Our results in toto support a model suggesting that during infection, the N and P proteins enter the nucleus independently, that viroplasm formation requires homologous N-protein interactions, and that P protein targeting to the viroplasm requires N-P protein interactions that occur after N and P protein import into the nucleus.
journal_name
J Viroljournal_title
Journal of virologyauthors
Deng M,Bragg JN,Ruzin S,Schichnes D,King D,Goodin MM,Jackson AOdoi
10.1128/JVI.02349-06subject
Has Abstractpub_date
2007-05-01 00:00:00pages
5362-74issue
10eissn
0022-538Xissn
1098-5514pii
JVI.02349-06journal_volume
81pub_type
杂志文章abstract::A total of 103 amber mutants of coliphage P1 were tested for lysis of nonpermissive cells. Of these, 83 caused cell lysis at the normal lysis time and have defects in particle morphogenesis. Five amber mutants, with mutations in the same gene (gene 2), caused premature lysis and may have a defect in a lysis regulator....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.2.519-530.1980
更新日期:1980-08-01 00:00:00
abstract::Two virus-specific species of newly synthesized DNA were isolated from rat fibroblast cell cultures infected with the Kilham rat virus (RV). These two DNA species were purified; their behavior on hydroxyapatite chromatography and their sedimentation coefficients in sucrose gradients were determined. One of the two spe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.20.1.86-95.1976
更新日期:1976-10-01 00:00:00
abstract::Hepatitis C virus (HCV) chronic infection is characterized by low-level or undetectable cellular immune responses against HCV antigens. HCV proteins have been shown to affect various intracellular events and modulate immune responses, although the precise mechanisms used to mediate these effects are not fully understo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02547-07
更新日期:2008-04-01 00:00:00
abstract::Newborn mice suckled on dams immunized either orally or parenterally with primate rotavirus SA-11 were protected against diarrhea induced by SA-11 virus challenge. Experimental oral administration of milk from orally immunized dams protected suckling mice against challenge; protective activity was detected both in the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.1.58-64.1985
更新日期:1985-04-01 00:00:00
abstract::Proteolytic processing of the Rous sarcoma virus (RSV) Gag precursor was altered in vivo through the introduction of amino acid substitutions into either the polyprotein cleavage junctions or the PR coding sequence. Single amino acid substitutions (V(P2)S and P(P4)G), which are predicted from in vitro peptide substrat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.3.2083-2091.1997
更新日期:1997-03-01 00:00:00
abstract::The foot-and-mouth disease virus (FMDV) capsid is highly acid labile, but introduction of amino acid replacements, including an N17D change in VP1, can increase its acid resistance. Using mutant VP1 N17D as a starting point, we isolated a virus with higher acid resistance carrying an additional replacement, VP2 H145Y,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03222-13
更新日期:2014-03-01 00:00:00
abstract::The herpes simplex virus 2 (HSV-2) viral microRNA (miRNA) designated miR-H6 is located upstream of the latency-associated transcript (LAT) promoter region on the strand opposite the LAT. Deletion of the LAT promoter and part of LAT exon 1 abolished HSV-2 miR-H6 expression in acutely and latently infected guinea pig do...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01997-10
更新日期:2011-05-01 00:00:00
abstract::Epstein-Barr virus (EBV) uses tonsils as the portal of entry to establish persistent infection. EBV is found in various B-cell subsets in tonsils but exclusively in memory B cells in peripheral blood. The in vitro susceptibilities of B-cell subsets to EBV infection have been studied solely qualitatively. In this work,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02630-07
更新日期:2008-05-01 00:00:00
abstract:UNLABELLED:Coronaviruses (CoVs) are unique in encoding a 3'→5' exoribonuclease within nonstructural protein 14 (nsp14-ExoN) that is required for high-fidelity replication, likely via proofreading. nsp14 associates with the CoV RNA-dependent RNA polymerase (nsp12-RdRp), and nsp14-ExoN activity is enhanced by binding nsp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00110-15
更新日期:2015-06-01 00:00:00
abstract::The ATP-dependent DNase activity of Escherichia coli disappeared or was markedly reduced after infection with double-stranded DNA phages, T2, T3, T4, T5, T6, T7, lambda, phi80, and P1, but not with the single-stranded DNA phage f1, or the RNA phage Qbeta. This DNase activity was not reduced when chloramphenicol was ad...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.6.1611-1612.1974
更新日期:1974-12-01 00:00:00
abstract::Only a few archaeal viruses have been subjected to detailed structural analyses. Major obstacles have been the extreme conditions such as high salinity or temperature needed for the propagation of these viruses. In addition, unusual morphotypes of many archaeal viruses have made it difficult to obtain further informat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01347-09
更新日期:2010-01-01 00:00:00
abstract:UNLABELLED:The HIV-1 envelope protein (Env) is heavily glycosylated, with approximately 50% of the Env molecular mass being contributed by N-glycans. HIV-1 Env N-glycans shield the protein backbone and have been shown to play key roles in determining Env structure, surface exposure, and, consequently, antigenicity, inf...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02164-14
更新日期:2014-12-01 00:00:00
abstract::A new purification procedure was adopted for Eastern equine encephalitis virus which does not subject the virus to pelleting at any stage. Three- to 4-liter volumes were passed through a diethylaminoethyl cellulose column. The virus-containing fractions were banded on a sucrose cushion and finally concentrated in an i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.2.233-240.1971
更新日期:1971-02-01 00:00:00
abstract::Infections of fungi by mycoviruses are often symptomless but sometimes also fatal, as they perturb sporulation, growth, and, if applicable, virulence of the fungal host. Hypovirulence-inducing mycoviruses, therefore, represent a powerful means to defeat fungal epidemics on crop plants. Infection with Fusarium graminea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00326-18
更新日期:2018-08-16 00:00:00
abstract::RNA transfer (Northern) blot analysis was used to perform the physical characterization of the transcript expressed in murine sensory nerve ganglia latently infected with herpes simplex virus type 1. Most of this latency-associated transcript (LAT) was isolated in the poly(A)- fraction from ganglia. A smaller RNA spec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.4.1194-1202.1988
更新日期:1988-04-01 00:00:00
abstract::Differential effect of various metabolic inhibitors on the development of hemadsorption activity and virus formation in cells infected with Newcastle disease virus (NDV) was investigated. It was found that, in BHK-21 cells infected with NDV, cycloheximide did not prevent the development of hemadsorption activity, wher...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.11.4.479-486.1973
更新日期:1973-04-01 00:00:00
abstract:UNLABELLED:Multiple cellular pathways are regulated by small ubiquitin-like modifier (SUMO) modification, including ubiquitin-mediated proteolysis, signal transduction, innate immunity, and antiviral defense. In the study described in this report, we investigated the effects of SUMO on the replication of two members of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00722-16
更新日期:2016-06-24 00:00:00
abstract::We have demonstrated that a liposomal array of well-ordered trimers enhances B cell activation, germinal center formation, and the elicitation of tier-2 autologous neutralizing antibodies. Previously, we coupled well-ordered cleavage-independent NFL trimers via their C-terminal polyhistidine tails to nickel lipids int...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00443-17
更新日期:2017-07-27 00:00:00
abstract::The gene encoding glycoprotein F (gF) of herpes simplex virus type 2 (HSV-2) was mapped to the region of the viral genome from 0.62 to 0.64 map units. This region is colinear with, and partially homologous to, the region of the HSV-1 genome previously shown to encode gC. Mapping of the gF gene was done by insertion of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.3.741-747.1984
更新日期:1984-03-01 00:00:00
abstract::NSP5 (NS26), the product of rotavirus gene 11, is a phosphoprotein whose role in the virus replication cycle is unknown. To gain further insight into its function, we obtained monoclonal antibodies against the baculovirus-expressed protein. By immunoprecipitation and immunoblotting experiments, we showed that (i) NSP5...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.34-41.1997
更新日期:1997-01-01 00:00:00
abstract::Recombinant viruses were constructed to have an Escherichia coli replicon containing a mutagenesis marker, the supF gene, integrated within the thymidine kinase locus (tk) of herpes simplex virus type 1. These viruses expressed either wild-type or mutant DNA polymerase (Pol) and were tested in a mutagenesis assay for ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.8.3605-3614.2002
更新日期:2002-04-01 00:00:00
abstract::The expression of a human immunodeficiency virus (HIV) type 1 provirus (F12-HIV) cloned from a nonproducer, chronically infected CD4 down-regulated Hut-78 cell clone (F12) does not lead to the formation of viral particles and, upon transfection in HeLa CD4+ cells, confers resistance to HIV superinfection without affec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.6618-6626.1995
更新日期:1995-11-01 00:00:00
abstract::Signal peptidase cleavage at the C-prM junction in the flavivirus structural polyprotein is inefficient in the absence of the cytoplasmic viral protease, which catalyzes cleavage at the COOH terminus of the C protein. The signal peptidase cleavage occurs efficiently in circumstances where the C protein is deleted or i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.2141-2149.1998
更新日期:1998-03-01 00:00:00
abstract::Epstein-Barr virus (EBV) transforms B lymphocytes through the expression of the latent viral proteins EBNA and latent membrane protein (LMP). Recently, it has become apparent that microRNAs (miRNAs) also contribute to EBV's oncogenic properties; recombinant EBVs that lack the BHRF1 miRNA cluster display a reduced abil...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05100-11
更新日期:2011-10-01 00:00:00
abstract::The E1A gene of highly oncogenic type 12 adenovirus (Ad12) possesses a segment unique to this serotype and comprising 60 base pairs contiguous with and separating conserved regions 2 and 3 in the gene. A similar but slightly longer segment is also present in the E1A gene of highly oncogenic simian adenovirus type 7 (D...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.877-887.1994
更新日期:1994-02-01 00:00:00
abstract::The cellular protein bone marrow stromal antigen-2 (BST-2)/tetherin acts against a variety of enveloped viruses by restricting their release from the plasma membrane. The HIV-1 accessory protein Vpu counteracts BST-2 by downregulating it from the cell surface and displacing it from virion assembly sites. Previous comp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02315-18
更新日期:2019-05-15 00:00:00
abstract::The herpes simplex virus type 1 capsid is composed of seven capsid proteins which are termed VP5, VP19c, VP21, VP22a, VP23, VP24, and VP26. Major capsid protein VP5 is encoded by the gene UL19. UL18, whose transcript is 3' coterminal with that of VP5, specifies capsid protein VP23. Vero cell lines have been isolated t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.3.1357-1364.1993
更新日期:1993-03-01 00:00:00
abstract::Removal of nucleoside triphosphates from extracts prepared from bacteriophage T7-infected Escherichia coli results in a stringent requirement for added ATP to form infective phage particles by in vitro packaging of bacteriophage T7 DNA. Optimal packaging efficiency was achieved at a concentration of about 1.25 mM. Oth...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.43.1.365-367.1982
更新日期:1982-07-01 00:00:00
abstract::The genome of murine cytomegalovirus, extracted from extracellular virions, is a linear double-stranded DNA molecule ca. 240 kilobase pairs long. In our initial cloning of subgenomic fragments of the murine cytomegalovirus genome, we obtained a HindIII clone which contained fused HindIII-terminal fragments. By hybridi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.1.24-28.1984
更新日期:1984-10-01 00:00:00
abstract::Caprine arthritis encephalitis virus (CAEV) is a lentivirus closely related to visna virus and more distantly to other lentiviruses, such as human immunodeficiency virus. The genomes of visna virus and CAEV contain a tat gene encoding a protein able to weakly transactivate its own long terminal repeat, suggesting that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.9.5445-5454.1995
更新日期:1995-09-01 00:00:00