Isolation and structural characterization of monomeric and dimeric forms of replicative intermediates of Kilham rat virus DNA.

Abstract:

:Two virus-specific species of newly synthesized DNA were isolated from rat fibroblast cell cultures infected with the Kilham rat virus (RV). These two DNA species were purified; their behavior on hydroxyapatite chromatography and their sedimentation coefficients in sucrose gradients were determined. One of the two species corresponds to the linear double-stranded form of the RV DNA, and the other corresponds to the dimeric duplex form. After denaturation, a fraction of both species showed an intramolecular renaturation; these molecules are composed of viral strand covalently linked to complementary strand. Models for the structure of both species are posposed. Both species may be considered as double-strand replicative intermediates of the single-stranded RV DNA.

journal_name

J Virol

journal_title

Journal of virology

authors

Gunther M,May P

doi

10.1128/JVI.20.1.86-95.1976

subject

Has Abstract

pub_date

1976-10-01 00:00:00

pages

86-95

issue

1

eissn

0022-538X

issn

1098-5514

journal_volume

20

pub_type

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