Abstract:
:A new purification procedure was adopted for Eastern equine encephalitis virus which does not subject the virus to pelleting at any stage. Three- to 4-liter volumes were passed through a diethylaminoethyl cellulose column. The virus-containing fractions were banded on a sucrose cushion and finally concentrated in an isopycnic band in a linear sucrose gradient. This method reduced the volume 1,000-fold with a concomitant increase in viral titer, i.e., better than 90% recovery. Numerous criteria have been used to establish that this viral preparation was essentially free from cellular debris and nonviral material. Physical studies on this purified viral product were initiated. The sedimentation coefficient as determined by band sedimentation was 240S, the buoyant density in sucrose was 1.18 g/cc, and the diameter of the virus was 54 nm. From the diameter and the buoyant density it was possible to calculate the molecular weight of a spherical particle. In this case, the calculated molecular weight for Eastern equine encephalitis virus was 58 x 10(6) daltons.
journal_name
J Viroljournal_title
Journal of virologyauthors
Fuscaldo AA,Aaslestad HG,Hoffman EJdoi
10.1128/JVI.7.2.233-240.1971subject
Has Abstractpub_date
1971-02-01 00:00:00pages
233-40issue
2eissn
0022-538Xissn
1098-5514journal_volume
7pub_type
杂志文章abstract::Synthesis of the vesicular stomatitis virus membrane matrix protein and the glycoprotein is inhibited to a greater extent than the synthesis of the nucleocapsid protein, the nonstructural protein, and the large protein when the rate of peptide chain initiation is reduced by exposure of vesicular stomatitis virus-infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.1.283-286.1976
更新日期:1975-01-01 00:00:00
abstract::A tumor line (58-2T) was established from a slowly growing tumor in a BALB/c mouse inoculated with M58-2 cells. The latter clonal cell line was isolated after bromodeoxyuridine treatment as a flat variant from nonproducer BALB/3T3 cells transformed by the Kirsten sarcoma virus. The 58-2T cells produced type C virus wi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.5.1262-1267.1974
更新日期:1974-11-01 00:00:00
abstract::For efficient and versatile use of adenovirus (Ad) as an in vivo gene therapy vector, modulation of the viral tropism is highly desirable. In this study, a novel method to genetically alter the Ad fiber tropism is described. The knob and the last 15 shaft repeats of the fiber gene were deleted and replaced with an ext...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.16.7280-7289.2001
更新日期:2001-08-01 00:00:00
abstract::In this study, we document that efficient interaction between arenavirus nucleoprotein (NP) and RNA-dependent RNA polymerase (L protein), the two trans-acting viral factors required for both virus RNA replication and gene transcription, requires the presence of virus-specific RNA sequences located within the untransla...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00103-15
更新日期:2015-05-01 00:00:00
abstract::We used 18 monoclonal antibodies against B19 parvovirus to identify neutralizing epitopes on the viral capsid. Of the 18 antibodies, 9 had in vitro neutralizing activity in a bone marrow colony culture assay. The overlapping polypeptide fragments spanning the B19 structural proteins were produced in a pMAL-c Escherich...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.12.7056-7060.1991
更新日期:1991-12-01 00:00:00
abstract::Hepatitis B virus particles contain three related viral envelope proteins, the small, middle, and large S (surface) proteins. All three proteins contain the small S amino acid sequence at their carboxyl terminus. It is not clear which of these S proteins functions as the viral attachment protein, binding to a target c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.5.1981-1988.1989
更新日期:1989-05-01 00:00:00
abstract::The recently emerged highly virulent variants of porcine epidemic diarrhea virus (PEDV) have caused colossal economic losses to the worldwide swine industry. In this study, we investigated the viral virulence determinants by constructing a series of chimeric mutants between the highly virulent strain BJ2011C and the a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00603-18
更新日期:2018-06-13 00:00:00
abstract::We have studied the fate of CD4 in CEM T cells expressing a human immunodeficiency virus type 1 HIV-1 Nef protein. Nef triggered a rapid endocytosis and a degradation of CD4, while most of the p56lck was upheld at the cell membrane. In the presence of Nef, CD4 accumulated in acidic intracellular vesicles that were not...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.1.528-533.1995
更新日期:1995-01-01 00:00:00
abstract::Intracellular simian virus 40 (SV40) chromatin was photoreacted with a 3H-labeled psoralen derivative, hydroxymethyltrimethylpsoralen (HMT), at 48 h postinfection. Psoralen compounds have been shown to readily penetrate intact cells and, in the presence of long-wavelength UV light, form covalent adducts to DNA, prefer...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.1.78-87.1982
更新日期:1982-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01345-10
更新日期:2010-12-01 00:00:00
abstract::Three drug-dependent mutants of human rhinovirus 16 (HRV16) were characterized by sequence analyses of spontaneous mutant isolates and were genetically reconstructed from a parental cDNA plasmid. These mutants formed plaques in the presence but not in the absence of the selecting antiviral drug, WIN 52035, which binds...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.2.1210-1218.1998
更新日期:1998-02-01 00:00:00
abstract::The glucocorticoid-regulatory sequences from the murine mammary tumor virus long terminal repeat (MMTV LTR) were introduced into the LTR of Moloney murine leukemia virus (M-MuLV) by recombinant DNA techniques. The site of insertion was in the M-MuLV LTR U3 region at -150 base pairs with respect to the RNA cap site. In...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.1.133-144.1985
更新日期:1985-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.7.4205-4209.1996
更新日期:1996-07-01 00:00:00
abstract::Activated microglia and astroglia are known to be involved in a variety of neurodegenerative diseases, including prion diseases. In the present experiments, we studied activation of astroglia and microglia after intraocular scrapie infection in transgenic mice expressing prion protein (PrP) in multiple cell types (tg7...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00865-07
更新日期:2007-10-01 00:00:00
abstract::Infection with human papillomavirus (HPV) is a critical factor in the pathogenesis of most cervical cancers and some aerodigestive cancers. The HPV E6 oncoprotein from high-risk HPV types contributes to the immortalization and transformation of cells by multiple mechanisms, including degradation of p53, transcriptiona...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01942-05
更新日期:2006-06-01 00:00:00
abstract::Complement, part of the innate immune system, acts to remove pathogens and unwanted host material. Complement is known to function in all tissues, including the central nervous system (CNS). In this study, we demonstrated the importance of the complement system within the CNS in the development of behavioral seizures ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00422-10
更新日期:2010-07-01 00:00:00
abstract::WUH4 is a highly pathogenic North American porcine reproductive and respiratory syndrome virus (PRRSV). Unlike previous PRRSV isolates, which were mainly recovered from sera or tissues, WUH4 was isolated from a piglet stool sample. Here we announce its complete genome sequence. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00055-12
更新日期:2012-04-01 00:00:00
abstract:UNLABELLED:Many viruses have the capacity to prevent a cell from being infected by a second virus, often termed superinfection exclusion. Alphaherpesviruses, including the human pathogen herpes simplex virus 1 (HSV-1) and the animal herpesvirus pseudorabies virus (PRV), encode a membrane-bound glycoprotein, gD, that ca...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00089-16
更新日期:2016-03-28 00:00:00
abstract:UNLABELLED:The increasing prevalence of ranavirus (RV; Iridoviridae) infections of wild and commercially maintained aquatic species is raising considerable concerns. While Xenopus laevis is the leading model for studies of immunity to RV, amphibian antiviral interferon (IFN) responses remain largely uncharacterized. Ac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00223-14
更新日期:2014-05-01 00:00:00
abstract::The secreted cytokine alpha/beta interferon (IFN-alpha/beta) binds its receptor to activate the Jak-STAT signal transduction pathway, leading to formation of the heterotrimeric IFN-stimulated gene factor 3 (ISGF3) transcription complex for induction of IFN-stimulated genes (ISGs) and establishment of an antiviral stat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01787-08
更新日期:2009-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.34.3.764-771.1980
更新日期:1980-06-01 00:00:00
abstract::Respiratory syncytial virus (RSV) is the major cause of acute bronchiolitis in infancy, a syndrome characterized by wheezing, respiratory distress, and the pathologic findings of peribronchial mononuclear cell infiltration and release of inflammatory mediators by basophil and eosinophil leukocytes. Composition and act...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.6.4756-4764.1998
更新日期:1998-06-01 00:00:00
abstract::While the facile transmission of chronic wasting disease (CWD) remains incompletely elucidated, studies in rodents suggest that exposure of the respiratory mucosa may be an efficient pathway. The present study was designed to address this question in the native cervid host. Here, we demonstrate aerosol transmission of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02852-12
更新日期:2013-02-01 00:00:00
abstract::BK polyomavirus (BKV) establishes persistent, low-level, and asymptomatic infections in most humans and causes polyomavirus-associated nephropathy (PVAN) and other pathologies in some individuals. The activation of BKV replication following kidney transplantation, leading to viruria, viremia, and, ultimately, PVAN, is...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06369-11
更新日期:2012-03-01 00:00:00
abstract::In mammalian cells, alphavirus replication complexes are anchored to the plasma membrane. This interaction with lipid bilayers is mediated through the viral methyl/guanylyltransferase nsP1 and reinforced by palmitoylation of cysteine residue(s) in the C-terminal region of this protein. Lipid content of membranes suppo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02183-19
更新日期:2020-05-04 00:00:00
abstract::The pathway of envelopment and egress of the varicella-zoster virus (VZV) and the primary site of viral production within the epidermal layer of the skin are not fully understood. There are several hypotheses to explain how the virus may receive an envelope as it travels to the surface of the monolayer. In this study,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.8.4994-5010.1995
更新日期:1995-08-01 00:00:00
abstract:UNLABELLED:To investigate the molecular mechanism(s) by which herpes simplex virus 1 (HSV-1) regulatory protein ICP0 promotes viral gene expression and replication, we screened cells overexpressing ICP0 for ICP0-binding host cell proteins. Tandem affinity purification of transiently expressed ICP0 coupled with mass spe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03043-15
更新日期:2016-01-06 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.4.3.313-314.1969
更新日期:1969-09-01 00:00:00
abstract::Interferon-induced proteins (IFPs) exert multiple functions corresponding to diverse interferon signals. However, the intracellular functions of many IFPs are not fully characterized. Here, we report that IFP35, a member of the IFP family with a molecular mass of 35 kDa, can interact with the bovine Tas (BTas) regulat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02249-07
更新日期:2008-05-01 00:00:00
abstract::Normal replication of Moloney murine leukemia virus (MoMLV) requires the integration of a DNA copy of the viral RNA genome into a chromosome of the host. In this work, we characterize the DNA sequences at the ends of the linear proviral precursor that are required for integration in the presence of MoMLV integration p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.11.5645-5648.1990
更新日期:1990-11-01 00:00:00