Abstract:
:Three drug-dependent mutants of human rhinovirus 16 (HRV16) were characterized by sequence analyses of spontaneous mutant isolates and were genetically reconstructed from a parental cDNA plasmid. These mutants formed plaques in the presence but not in the absence of the selecting antiviral drug, WIN 52035, which binds to the capsid of wild-type virus and inhibits its attachment to the host cell. The drug-dependent phenotype of each mutant was caused by a single amino acid substitution in the VP1 coat protein. The three independent mutations conferring drug dependence are M1103T, T1208A, and V1210A. Single-step growth experiments involving rescue of one of the three mutants (V1210A) by delayed drug addition suggested (i) that the drug dependence lesion is at the stage of virus assembly and (ii) that one or more components of the viral assembly pool decay in the absence of drug. RNA accumulation and infectivity were unaffected by the absence of drug in all three mutants, suggesting that the labile assembly component is coat protein.
journal_name
J Viroljournal_title
Journal of virologyauthors
Wang W,Lee WM,Mosser AG,Rueckert RRdoi
10.1128/JVI.72.2.1210-1218.1998subject
Has Abstractpub_date
1998-02-01 00:00:00pages
1210-8issue
2eissn
0022-538Xissn
1098-5514journal_volume
72pub_type
杂志文章abstract::A variant form of splicing of RNA crossing the Epstein-Barr virus (EBV) BZLF1 gene was observed in the late productive cycle of EBV. This splice omits the middle exon of BZLF1 and joins the outer two exons of BZLF1 in frame, but the shortened form of BZLF1 protein (Z delta) could not be detected in natural EBV infecti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.10.6233-6236.1992
更新日期:1992-10-01 00:00:00
abstract::Alphavirus expression systems based on suicidal virus particles carrying recombinant replicons have proven to be a very efficient way to deliver genes for heterologous protein expression. However, present strategies for production of such particles have biosafety limitations due to the generation, by RNA recombination...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.2.1092-1098.1999
更新日期:1999-02-01 00:00:00
abstract::When short pulses of [(3)H]uracil were administered to Bacillus subtilis infected with phage 2C, the main species of labeled RNA was a 10S component that hybridized chiefly, but not exclusively, with the heavy strand of 2C DNA. After long pulses, most of the radioactivity was found in the 23S, 16S, and 5S rRNA's, whic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.6.1482-1493.1974
更新日期:1974-12-01 00:00:00
abstract::Although several virologic and immunologic factors associated with an increased risk of perinatal human immunodeficiency virus type 1 (HIV-1) transmission have been described, the mechanism of mother-to-child transmission is still unclear. More specifically, the question of whether selective pressures influence the tr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.5.3050-3057.2003
更新日期:2003-03-01 00:00:00
abstract::Rotavirus plus-strand RNAs not only direct protein synthesis but also serve as templates for the synthesis of the segmented double-stranded RNA (dsRNA) genome. In this study, we identified short-interfering RNAs (siRNAs) for viral genes 5, 8, and 9 that suppressed the expression of NSP1, a nonessential protein; NSP2, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.14.7763-7774.2004
更新日期:2004-07-01 00:00:00
abstract::F proteins from baculovirus nucleopolyhedrovirus (NPV) group II members are the major budded virus (BV) viral envelope fusion proteins. They undergo furin-like proteolysis processing in order to be functional. F proteins from different baculovirus species have a long cytoplasmic tail domain (CTD), ranging from 48 (Spo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01178-06
更新日期:2006-11-01 00:00:00
abstract::Three polyadenylated RNAs, 9, 7.3, and 3.5 kilobases long, of a human endogenous retrovirus, ERV3, are abundant in human placental chorion, representing about 0.03 to 0.05% of the total mRNA. We characterized the structure of these mRNAs by Northern blot and S1 nuclease mapping analyses. We found that all three RNAs w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.7.2182-2191.1987
更新日期:1987-07-01 00:00:00
abstract::Three of thirty-six monoclonal antibodies were found to cause irreversible inactivation of type 1 poliovirus at 39 degrees C but not at 37 degrees C. Neutralization at 37 degrees C depended on aggregation and was reversible by acid-induced deaggregation; at 39 degrees C, the virions (N antigenic, 160S) were disrupted ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.9.5299-5302.1993
更新日期:1993-09-01 00:00:00
abstract:UNLABELLED:Little is known about the antiviral response in mollusks. As in other invertebrates, the interferon signaling pathways have not been identified, and in fact, there is a debate about whether invertebrates possess antiviral immunity similar to that of vertebrates. In marine bivalves, due to their filtering act...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00591-16
更新日期:2016-08-12 00:00:00
abstract::The neuraminidase inhibitors zanamivir and oseltamivir are marketed for the treatment and prophylaxis of influenza and have been stockpiled by many countries for use in a pandemic. Although recent surveillance has identified a striking increase in the frequency of oseltamivir-resistant seasonal influenza A (H1N1) viru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01200-09
更新日期:2009-10-01 00:00:00
abstract::The vast majority of people already have preexisting immune responses to influenza viruses from one or more subtypes. However, almost all preclinical studies evaluate new influenza vaccine candidates in immunologically naive animals. Recently, our group demonstrated that priming naive ferrets with broadly reactive H1 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00946-18
更新日期:2019-01-17 00:00:00
abstract::A general theme that has emerged from studies of DNA tumor viruses is that otherwise unrelated oncoproteins encoded by these viruses often target the same important cellular factors. Major oncogenic determinants for human adenovirus type 9 (Ad9) and high-risk human papillomaviruses (HPV) are the E4-ORF1 and E6 oncopro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.20.9680-9693.2000
更新日期:2000-10-01 00:00:00
abstract::The influenza A virus (IAV) envelope protein hemagglutinin binds α2,6- or α2,3-linked sialic acid as a host cell receptor. Bat IAV subtypes H17N10 and H18N11 form an exception to this rule and do not bind sialic acid but enter cells via major histocompatibility complex (MHC) class II. Here, we review current knowledge...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.01357-19
更新日期:2020-01-31 00:00:00
abstract:UNLABELLED:Transmission of chronic wasting disease (CWD) between cervids is influenced by the primary structure of the host cellular prion protein (PrP(C)). In white-tailed deer, PRNP alleles encode the polymorphisms Q95 G96 (wild type [wt]), Q95 S96 (referred to as the S96 allele), and H95 G96 (referred to as the H95 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02010-15
更新日期:2015-12-01 00:00:00
abstract::Hepatitis E virus genotype 1 strain Sar55 replicated in subcloned Caco-2 intestinal cells and Huh7 hepatoma cells that had been transfected with in vitro transcribed viral genomes, and hepatitis E virions were released into the culture medium of both cell lines. Virus egress from cells depended on open reading frame 3...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00593-10
更新日期:2010-09-01 00:00:00
abstract::In this paper, we report that the DNA of bovine mammillitis virus (BMV) consists of two covalently linked components that are 71.5 x 10(6) and 15.7 x 10(6) in molecular weight and designated L and S, respectively. We further report that the BMV DNA consists of four equimolar populations differing only in the orientati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.1.395-407.1978
更新日期:1978-01-01 00:00:00
abstract::The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) glycoprotein GP64 is the major envelope protein of the budded virus (BV). GP64 is a class III fusion protein that mediates BV attachment to the cell surface and low-pH-triggered membrane fusion between the BV envelope and the endosome membrane during en...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00813-12
更新日期:2012-09-01 00:00:00
abstract::The C-terminal domain of human immunodeficiency virus type 1 (HIV-1) integrase (IN) is a dimer that binds to DNA in a nonspecific manner. The structure of the minimal region required for DNA binding (IN220-270) has been solved by nuclear magnetic resonance spectroscopy. The overall fold of the C-terminal domain of HIV...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.6.4841-4848.1998
更新日期:1998-06-01 00:00:00
abstract::Nonstructural protein 5B (NS5B) of bovine viral diarrhea virus (BVDV) contains sequence motifs that are predictive of an RNA-dependent RNA polymerase activity. We describe the expression and purification of the BVDV NS5B protein derived from an infectious cDNA clone of BVDV (NADL strain). BVDV NS5B protein was active ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.9365-9369.1998
更新日期:1998-11-01 00:00:00
abstract::The key role of the mitochondria in the regulation of cellular Ca2+ led to a study of mitochondrial Ca2+ uptake during the infection of chicken embryo cells with Semliki Forest virus. Mitochondrial Ca2+ uptake was stimulated during the first 5 h of infection but declined later in infection. The early stimulation sugge...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.1.143-152.1979
更新日期:1979-01-01 00:00:00
abstract::Viruses actively interact with host metabolism because viral replication relies on host cells to provide nutrients and energy. Vaccinia virus (VACV; the prototype poxvirus) prefers glutamine to glucose for efficient replication to the extent that VACV replication is hindered in glutamine-free medium. Remarkably, our d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01834-18
更新日期:2019-06-14 00:00:00
abstract::Major neutralization antigenic sites have been previously mapped by us on VP1, the largest capsid protein of poliovirus type 1. Here we report the first identification of the primary sequence of a neutralization antigenic site on capsid protein VP2. Inspection of the amino acid sequence of VP2 led to the selection and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.2.719-721.1984
更新日期:1984-11-01 00:00:00
abstract::RNA packaging signals (psi) from the 5' ends of murine and avian retroviral genomes have previously been shown to direct encapsidation of heterologous mRNA into the retroviral virion. The avian 5' packaging region has now been further characterized, and we have defined a 270-nucleotide sequence, A psi, which is suffic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.1.178-188.1993
更新日期:1993-01-01 00:00:00
abstract::The locations of replication pause sites in the simian virus 40 minichromosome which were determined by sizing cloned fragments of nascent DNA (Zannis-Hadjopoulos et al., J. Mol. Biol. 165:599-607, 1983) were compared with the positions of simian virus 40 nucleosomes in the genome, as obtained by sequence-directed map...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.3.1011-1012.1984
更新日期:1984-12-01 00:00:00
abstract::Using a yeast two-hybrid screen of a T-cell cDNA library to identify cellular proteins that bind to the human immunodeficiency virus type 2 (HIV-2) Gag polyprotein, we identified PRP4, a serine-threonine protein kinase. Specific interaction of PRP4 and HIV-2 Gag was confirmed in in vitro and in vivo assays. The intera...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.20.11303-11312.2004
更新日期:2004-10-01 00:00:00
abstract::Echovirus 1 (EV1) is a human pathogen which belongs to the Picornaviridae family of RNA viruses. We have analyzed the early events of infection after EV1 binding to its receptor alpha 2 beta 1 integrin and elucidated the route by which EV1 gains access to the host cell. EV1 binding onto the cell surface and subsequent...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.4.1856-1865.2002
更新日期:2002-02-01 00:00:00
abstract::We have previously shown that expression of the adenovirus E1A 12S or 13S products in NIH 3T3 fibroblasts induces susceptibility to the cytotoxic actions of tumor necrosis factor alpha (TNF alpha). A large number of studies have mapped the multiple biological functions of the 12S and 13S products to three highly conse...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.9.4115-4122.1990
更新日期:1990-09-01 00:00:00
abstract::At mucosal sites, papillomavirus genomes can persist in the epithelial basal layer following immune-mediated regression. Subsequent T-cell depletion stimulates a 3- to 5-log increase in the viral copy number, to levels associated with productive infection. Reappearance of microlesions was rare within the short time fr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02589-13
更新日期:2014-01-01 00:00:00
abstract::Hepatitis C virus (HCV) has two envelope proteins, E1 and E2, which form a heterooligomer. During dissection of interacting regions of HCV E1 and E2, we found the presence of an interfering compound or compounds in skim milk. Here we report that human as well as bovine lactoferrin, a multifunctional immunomodulator, b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.8.5997-6002.1997
更新日期:1997-08-01 00:00:00
abstract::In most human immunodeficiency virus type 1 (HIV-1)-infected individuals who achieve viral loads of <50 copies/ml during highly active antiretroviral therapy (HAART), low levels of plasma virus remain detectable for years by ultrasensitive methods. The relative contributions of ongoing virus replication and virus prod...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00570-09
更新日期:2009-10-01 00:00:00