Avian retroviral RNA encapsidation: reexamination of functional 5' RNA sequences and the role of nucleocapsid Cys-His motifs.

abstract：:ZAM is an env-containing member of the gypsy family of retrotransposons that represents a possible retrovirus of invertebrates. In this paper, we traced ZAM mobilization to get information about a potential path a retroelement may take to reach the germ line of its host. In situ hybridization on whole-mount tissues an...

journal_title：Journal of virology

authors： Leblanc P,Desset S,Giorgi F,Taddei AR,Fausto AM,Mazzini M,Dastugue B,Vaury C

更新日期：2000-11-01 00:00:00

abstract：:A short sequence, located between the A14L and A15L open reading frames (ORFs) of vaccinia virus, was predicted to encode a hydrophobic protein of 53 amino acids that is conserved in orthopoxviruses, leporipoxviruses, yatapoxiruses, and molluscipoxviruses. We constructed a recombinant vaccinia virus with a 10-codon ep...

journal_title：Journal of virology

authors： Betakova T,Wolffe EJ,Moss B

更新日期：2000-05-01 00:00:00

abstract：:Purified Friend viruses obtained from chronically infected tissue cultures were studied under the electron microscope in an effort to correlate the fine structure of the particles to their leukemogenic activity under varied experimental conditions, i.e., temperature treatments and exposure to Tween 80, amyl acetate, o...

journal_title：Journal of virology

authors： De Tkaczevski L,De Harven E,Friend C

更新日期：1968-04-01 00:00:00

abstract：:A cDNA fragment coding for poliovirus capsid polypeptide VP1 was inserted into a simian virus 40 (SV40) genome in the place of the SV40 VP1 gene and fused in phase to the 3' end of the VP2-VP3 genes. Simian cells were infected with the resulting hybrid virus in the presence of an early SV40 mutant used as a helper. In...

journal_title：Journal of virology

authors： Wychowski C,Benichou D,Girard M

更新日期：1987-12-01 00:00:00

abstract：:The synthesis of membrane protein after infection with bacteriophage T4 was examined. Protein constituents of both the cytoplasmic and outer membrane are made during the infective cycle. In addition, newly synthesized membrane protein is found in material which has a buoyant density greater than that of either of the ...

journal_title：Journal of virology

authors： Fletcher G,Wulff JL,Earhart CF

更新日期：1974-01-01 00:00:00

abstract：:AV3 cells (continuous human amnion) infected with the Onderstepoort strain of canine distemper virus produced cell fusion within 2 to 5 hr when added to AV3 cell monolayers. An apparent requirement for intact, infected cells was demonstrated by showing that (i) frozen-and-thawed infected cells failed to induce fusion,...

journal_title：Journal of virology

authors： Rankin AM,Fisher LE,Bussell RH

更新日期：1972-12-01 00:00:00

abstract：:Influenza virus defective interfering (DI) particles are naturally occurring noninfectious virions typically generated during in vitro serial passages in cell culture of the virus at a high multiplicity of infection. DI particles are recognized for the role they play in inhibiting viral replication and for the impact ...

journal_title：Journal of virology

authors： Saira K,Lin X,DePasse JV,Halpin R,Twaddle A,Stockwell T,Angus B,Cozzi-Lepri A,Delfino M,Dugan V,Dwyer DE,Freiberg M,Horban A,Losso M,Lynfield R,Wentworth DN,Holmes EC,Davey R,Wentworth DE,Ghedin E,INSIGHT FLU002 S

更新日期：2013-07-01 00:00:00

abstract：:Propagation of the flavivirus tick-borne encephalitis virus in BHK-21 cells selected for mutations within the large surface glycoprotein E that increased the net positive charge of the protein. In the course of 16 independent experiments, 12 different protein E mutation patterns were identified. These were located in ...

journal_title：Journal of virology

authors： Mandl CW,Kroschewski H,Allison SL,Kofler R,Holzmann H,Meixner T,Heinz FX

更新日期：2001-06-01 00:00:00

abstract：:Today, global attention is focused on two influenza virus strains: the current pandemic strain, swine origin influenza virus (H1N1-2009), and the highly pathogenic avian influenza virus, H5N1. At present, the infection caused by the H1N1-2009 is moderate, with mortality rates of less <1%. In contrast, infection with t...

journal_title：Journal of virology

authors： Achdout H,Meningher T,Hirsh S,Glasner A,Bar-On Y,Gur C,Porgador A,Mendelson M,Mandelboim M,Mandelboim O

更新日期：2010-04-01 00:00:00

abstract：:Zika virus (ZIKV) envelope (E) protein is the major target of neutralizing antibodies in infected hosts and thus represents a candidate of interest for vaccine design. However, a major concern in the development of vaccines against ZIKV and the related dengue virus is the induction of cross-reactive poorly neutralizin...

journal_title：Journal of virology

authors： De Lorenzo G,Tandavanitj R,Doig J,Setthapramote C,Poggianella M,Sanchez-Velazquez R,Scales HE,Edgar JM,Kohl A,Brewer J,Burrone OR,Patel AH

更新日期：2020-12-09 00:00:00

abstract：:Three polyadenylated RNAs, 9, 7.3, and 3.5 kilobases long, of a human endogenous retrovirus, ERV3, are abundant in human placental chorion, representing about 0.03 to 0.05% of the total mRNA. We characterized the structure of these mRNAs by Northern blot and S1 nuclease mapping analyses. We found that all three RNAs w...

journal_title：Journal of virology

authors： Kato N,Pfeifer-Ohlsson S,Kato M,Larsson E,Rydnert J,Ohlsson R,Cohen M

更新日期：1987-07-01 00:00:00

abstract：:The paramyxoviral family contains many medically important viruses, including measles virus, mumps virus, parainfluenza viruses, respiratory syncytial virus, human metapneumovirus, and the deadly zoonotic henipaviruses Hendra and Nipah virus (NiV). To both enter host cells and spread from cell to cell within infected ...

journal_title：Journal of virology

authors： Stone JA,Vemulapati BM,Bradel-Tretheway B,Aguilar HC

更新日期：2016-11-14 00:00:00

abstract：:The genome of the simian virus 40 (SV40) temperature-sensitive (ts) mutant tsD202 rescued by passage on transformed permissive monkey lines (see accompanying paper [Y. Gluzman et al., J. Virol. 24:534-540, 1977]) was analyzed by restriction endonuclease cleavage mapping to obtain biochemical evidence that the rescue o...

journal_title：Journal of virology

authors： Vogel T,Gluzman Y,Winocour E

更新日期：1977-11-01 00:00:00

abstract：:Treatment of polyoma virions with ethyleneglycol-bil-N,N'-tetraacetic acid (EGTA) and dithiothreitol (DTT) at pH 8.5 resulted in the dissociation of the virions into a DNA-protein complex and individual structural capsomere subunits. The sedimentation value of the DNA-protein complex in sucrose gradients was approxima...

journal_title：Journal of virology

authors： Brady JN,Winston VD,Consigli RA

更新日期：1978-07-01 00:00:00

abstract：:The Ebola virus glycoprotein mucin-like domain (MLD) is implicated in Ebola virus cell entry and immune evasion. Using cryo-electron tomography of Ebola virus-like particles, we determined a three-dimensional structure for the full-length glycoprotein in a near-native state and compared it to that of a glycoprotein la...

journal_title：Journal of virology

authors： Tran EE,Simmons JA,Bartesaghi A,Shoemaker CJ,Nelson E,White JM,Subramaniam S

更新日期：2014-09-01 00:00:00

abstract：:Bacteriophages of the C3 morphotype, characterized by very long heads that exceed their width several times, are extremely rare among the Podoviridae family members and constitute only 0.5% of over 5,500 phages that have been examined by the electron microscope (H. W. Ackermann, Arch. Virol. 152:227-243, 2007; H. W. A...

journal_title：Journal of virology

authors： Li Y,Chen M,Tang F,Yao H,Lu C,Zhang W

更新日期：2012-12-01 00:00:00

abstract：:Viral infection induces production of type I interferons (IFNs), which stimulate the expression of a variety of antiviral factors to inhibit viral replication. To establish effective infection, viruses need to develop strategies to evade the immune responses. A neurovirulent Sindbis virus strain with neuroinvasive pro...

journal_title：Journal of virology

authors： Wang X,Li MMH,Zhao J,Li S,MacDonald MR,Rice CM,Gao X,Gao G

更新日期：2016-10-28 00:00:00

abstract：:We have studied initiation and regulation of early transcription of simian virus (SV40) DNA in vitro by eucaryotic RNA polymerase II, using both a crude HeLa cell extract and a partially purified calf thymus polymerase supplemented with a HeLa cell S100 fraction. Analysis of initiation sites by primer-directed cDNA sy...

journal_title：Journal of virology

authors： Lebowitz P,Ghosh PK

更新日期：1982-02-01 00:00:00

abstract：:Human hepatitis B virus (HBV) HBx protein is a multifunctional protein that activates cellular signaling pathways and is thought to be essential for viral infection. Woodchuck HBV mutants that lack HBx are unable to replicate in vivo or are severely impaired. HBV replication in HepG2 cells, a human hepatoblastoma cell...

journal_title：Journal of virology

authors： Bouchard MJ,Puro RJ,Wang L,Schneider RJ

更新日期：2003-07-01 00:00:00

abstract：:The process of Epstein-Barr virus (EBV)-induced transformation of human B lymphocytes results in a cell line that is a mixture of latently and lytically infected cells, with the lytic cells composing roughly 5% to less than 0.0001% of the overall population. A set of nine normal lymphoblastoid cell lines that span a 1...

journal_title：Journal of virology

authors： Metzenberg S

更新日期：1990-01-01 00:00:00

abstract：:Due to various advantageous features there is current interest in retroviral vectors derived from primate foamy viruses (PFVs). Two PFV cis-acting sequences have been mapped in the 5' region of the RNA (pre-)genome and in the 3' pol genomic region. In order to genetically separate PFV packaging constructs from vector ...

journal_title：Journal of virology

authors： Heinkelein M,Thurow J,Dressler M,Imrich H,Neumann-Haefelin D,McClure MO,Rethwilm A

更新日期：2000-04-01 00:00:00

abstract：UNLABELLED:We have recently shown in both herpesviruses and phages that packaged viral DNA creates a pressure of tens of atmospheres pushing against the interior capsid wall. For the first time, using differential scanning microcalorimetry, we directly measured the energy powering the release of pressurized DNA from th...

journal_title：Journal of virology

authors： Bauer DW,Li D,Huffman J,Homa FL,Wilson K,Leavitt JC,Casjens SR,Baines J,Evilevitch A

更新日期：2015-09-01 00:00:00

abstract：:The herpes simplex virus type 1 (HSV-1) tegument is the least understood component of the virion, and the mechanism of tegument assembly and incorporation into virions during viral egress has not yet been elucidated. In the present study, the addition of tegument proteins (VP13/14, VP16, VP22, and US9) and envelope gl...

journal_title：Journal of virology

authors： Miranda-Saksena M,Boadle RA,Armati P,Cunningham AL

更新日期：2002-10-01 00:00:00

abstract：:Polydnaviridae is a unique family of DNA viruses that are symbiotically associated with parasitoid wasps. Upon oviposition, wasps inject these viruses into their hosts, where they cause several physiological alterations, including suppression of the cellular immune response. Here we report that expression of the glc1....

journal_title：Journal of virology

authors： Beck M,Strand MR

更新日期：2005-02-01 00:00:00

abstract：:Group A rotaviruses are major pathogens causing acute gastroenteritis in children and animals. To determine if group A rotavirus replicates and induces disease in rats, antibody-negative Lewis neonatal or adult rats were inoculated orally with tissue culture-adapted human (Wa, WI61, and HAL1166), simian (rhesus rotavi...

journal_title：Journal of virology

authors： Ciarlet M,Conner ME,Finegold MJ,Estes MK

更新日期：2002-01-01 00:00:00

abstract：:We have previously demonstrated that two mouse cell lines that are poorly infectible by reovirus become highly susceptible upon transfection with the gene encoding the epidermal growth factor receptor (EGFR) (J. E. Strong, D. Tang, and P. W. K. Lee, Virology 197:405-411, 1993). This enhancement of infection efficiency...

journal_title：Journal of virology

authors： Strong JE,Lee PW

更新日期：1996-01-01 00:00:00

abstract：:We have previously characterized five proteins induced by the presence of the E2 open reading frame (ORF) region of bovine papillomavirus type 1 (BPV-1) in C127 mouse fibroblasts (R. M. Levenson, U. G. Brinckmann, M. K. O'Banion, E. J. Androphy, J. T. Schiller, F. Tabatabai, L. P. Turek, K. Neary, M. T. Chin, T. R. Br...

journal_title：Journal of virology

authors： O'Banion MK,Young DA

更新日期：1991-07-01 00:00:00

abstract：:Six phages were isolated from sewage and from a lysogenic strain. Three of them, belonging to a new morphological group, had contractile tails and elongated heads with axial ratios of 2.4:1. Two phages, possessing short tails and very long heads with axial ratios of 3.5:1, were new isolates of an extremely rare group....

journal_title：Journal of virology

authors： Ackermann HW,Petrow S,Kasatiya SS

更新日期：1974-03-01 00:00:00

abstract：:Using quantitative RNase protection assays, we have monitored the appearance of mRNAs generated during lytic infection of tightly synchronized murine cells by the autonomous parvovirus minute virus of mice. Our results demonstrate that transcripts from the P4 promoter can be detected prior to those from the P39 promot...

journal_title：Journal of virology

authors： Clemens KE,Pintel DJ

更新日期：1988-04-01 00:00:00

abstract：:The herpes simplex virus (HSV) virion host shutoff (vhs) protein (UL41 gene product) is a component of the HSV virion tegument that triggers shutoff of host protein synthesis and accelerated mRNA degradation during the early stages of HSV infection. vhs displays weak amino acid sequence similarity to the fen-1 family ...

journal_title：Journal of virology

authors： Elgadi MM,Smiley JR

更新日期：1999-11-01 00:00:00