Abstract:
:We have previously shown that expression of the adenovirus E1A 12S or 13S products in NIH 3T3 fibroblasts induces susceptibility to the cytotoxic actions of tumor necrosis factor alpha (TNF alpha). A large number of studies have mapped the multiple biological functions of the 12S and 13S products to three highly conserved regions (CR) within the E1A sequence. Here we used plasmids coding for E1A deletion and point mutants in these regions to generate target cell lines for TNF alpha cytotoxicity assays to determine which regions and functions are necessary for the induction of TNF alpha sensitivity. Expression of CR1 was required for the induction of TNF alpha sensitivity. This finding did not reflect a requirement for transforming or transcriptional repression activity, since some mutants that were defective in both of these properties were able to induce TNF alpha sensitivity. CR2 transformation-defective point mutants, but not a CR2/3 region deletion mutant, were also able to induce sensitivity. In addition, NIH 3T3 cells expressing the retroviral transcription activators tat from human immunodeficiency virus type 1 and tax from human T-lymphotropic virus type I were not sensitive to TNF alpha. However, the possibility that E1A-mediated transcriptional activation can augment the induction of TNF alpha sensitivity is not excluded. Comparison of data from previous biological studies with the TNF alpha cytotoxicity assays presented here suggested that the mechanism by which E1A induces sensitivity to TNF alpha in NIH 3T3 cells is independent of many of the known E1A biological functions, including transformation in cooperation with ras, immortalization, induction of DNA synthesis in quiescent cells, and transcriptional repression. A novel E1A-mediated effect may be involved, although our data do not exclude the possibility that sensitization to TNF alpha is mediated through E1A binding to cellular proteins.
journal_name
J Viroljournal_title
Journal of virologyauthors
Ames RS,Holskin B,Mitcho M,Shalloway D,Chen MJdoi
10.1128/JVI.64.9.4115-4122.1990subject
Has Abstractpub_date
1990-09-01 00:00:00pages
4115-22issue
9eissn
0022-538Xissn
1098-5514journal_volume
64pub_type
杂志文章abstract::Levels of the p53 tumor suppressor protein are increased in human cytomegalovirus (HCMV)-infected cells and may be important for HCMV pathogenesis. In normal cells p53 levels are kept low due to an autoregulatory feedback loop where p53 activates the transcription of mdm2 and mdm2 binds and ubiquitinates p53, targetin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.8.3833-3843.2006
更新日期:2006-04-01 00:00:00
abstract:UNLABELLED:CD4+ T cells play a pivotal role in the control of chronic viral infections. Recently, nontraditional CD4+ T cell functions beyond helper effects have been described, and a role for cytolytic CD4+ T cells in the control of HIV infection has been suggested. We define here the transcriptional, phenotypic, and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00438-15
更新日期:2015-08-01 00:00:00
abstract::The stimulation of host macromolecular synthesis and induction into the cell cycle of serum-deprived G0-G1-arrested mouse embryo fibroblasts were examined after infection of resting cells with wild-type simian virus 40 or with viral mutants affecting T antigen (tsA58) or small t antigen (dl884). At various times after...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.2.802-812.1981
更新日期:1981-02-01 00:00:00
abstract::The ability of certain vesicular stomatitis virus (VSV; Indiana serotype) temperature-sensitive (ts) mutants to synthesize intracellular viral complementary RNA (vcRNA) at permissive or nonpermissive temperatures for productive infections has been investigated. Mutants belonging to complementation groups II, III, and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.20.1.157-169.1976
更新日期:1976-10-01 00:00:00
abstract::A substantial number of viruses have been demonstrated to subvert autophagy to promote their own replication. Recent publications have reported the proviral effect of autophagy induction on hepatitis B virus (HBV) replication. Hepatitis delta virus (HDV) is a defective virus and an occasional obligate satellite of HBV...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01936-19
更新日期:2020-01-31 00:00:00
abstract::New zonal centrifuges can conveniently process as much as five orders of magnitude (10(5)) greater sample volumes than conventional swinging-bucket rotors. The continuous-sample-flow-with-banding versions may be used in series with ancillary purification procedures. Here we have studied the combined process: absorptio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.1.6.1207-1216.1967
更新日期:1967-12-01 00:00:00
abstract::We describe here the neurovirulence properties of a herpes simplex virus type 1 gamma34.5 second-site suppressor mutant. gamma34.5 mutants are nonneurovirulent in animals and fail to grow in a variety of cultured cells due to a block at the level of protein synthesis. Extragenic suppressors with restored capacity to r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.11.5189-5196.2001
更新日期:2001-06-01 00:00:00
abstract::Early stages of infection by the mouse polyomavirus have been studied using HeLa cells stably expressing small interfering RNA to protein disulfide isomerase (PDI). Infectibility measured by nuclear T antigen expression was reduced commensurately with the degree of PDI downregulation. Infectibility was restored by tra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01117-06
更新日期:2006-11-01 00:00:00
abstract:UNLABELLED:JC polyomavirus (JCPyV) infection of immunocompromised individuals results in the fatal demyelinating disease progressive multifocal leukoencephalopathy (PML). The viral capsid of JCPyV is composed primarily of the major capsid protein virus protein 1 (VP1), and pentameric arrangement of VP1 monomers results...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00089-15
更新日期:2015-04-01 00:00:00
abstract::The cloned transcription factor hepatocyte nuclear factor 1 (HNF1) transactivates transcription from the hepatitis B virus (HBV) large surface antigen promoter but does not influence the transcriptional activities of the other three HBV promoters. This indicates that this transcription factor can differentially influe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.11.5774-5781.1991
更新日期:1991-11-01 00:00:00
abstract::The 70S RNA of Rous sarcoma virus contains 4S RNAs which serve as primers for the initiation of DNA synthesis in vitro by the RNA-directed DNA polymerase of the virus. We purified these primers in three different ways-by isolation of the covalent complex between primer and nascent DNA, by differential melting of the 7...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.5.1134-1142.1974
更新日期:1974-05-01 00:00:00
abstract::The Epstein-Barr virus oriP, when examined with the use of in vitro nucleosome reconstitution, was found to exhibit a partial sparing of nucleosome assembly within the 3' dyad. Analysis of the homologous region containing the origin of replication of herpesvirus papio disclosed that sparing of nucleosome assembly was ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.12.5505-5508.1989
更新日期:1989-12-01 00:00:00
abstract::Purified Friend viruses obtained from chronically infected tissue cultures were studied under the electron microscope in an effort to correlate the fine structure of the particles to their leukemogenic activity under varied experimental conditions, i.e., temperature treatments and exposure to Tween 80, amyl acetate, o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.4.365-375.1968
更新日期:1968-04-01 00:00:00
abstract::The contribution of the hepatitis B virus enhancers I and II in the regulation of the activity of the core and the X promoters was assessed in transgenic mice. Surprisingly, despite the presence of heterologous promoters linked 5' of the X gene, the transgene expression is mostly due to core promoter (Cp) activity pre...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.9.5912-5916.1995
更新日期:1995-09-01 00:00:00
abstract::We generated a recombinant influenza A virus (Mmut) that produced low levels of matrix (M1) and M2 proteins in infected cells. Mmut virus propagated to significantly lower titers than did wild-type virus in cells infected at low multiplicity. By contrast, virion morphology and incorporation of viral proteins and vRNAs...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.12.7926-7932.2005
更新日期:2005-06-01 00:00:00
abstract::CD81 has been described as a putative receptor for hepatitis C virus (HCV); however, its role in HCV cell entry has not been characterized due to the lack of an efficient cell culture system. We have examined the role of CD81 in HCV glycoprotein-dependent entry by using a recently developed retroviral pseudotyping sys...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.3.1448-1455.2004
更新日期:2004-02-01 00:00:00
abstract::During viral infection, inflammation and recovery are tightly controlled by competing proinflammatory and regulatory immune pathways. Respiratory syncytial virus (RSV) is the leading global cause of infantile bronchiolitis, which is associated with recurrent wheeze and asthma diagnosis in later life. Th2-driven diseas...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01295-13
更新日期:2013-10-01 00:00:00
abstract::The global eradication of poliomyelitis will require substantial changes in immunization practices. One of the proposed scenarios includes cessation of vaccination with live oral poliovirus vaccine (OPV) and the creation of an OPV stockpile for emergency response in case of the reintroduction of poliovirus into circul...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.23.12460-12465.2003
更新日期:2003-12-01 00:00:00
abstract::To facilitate the determination of the genomic location of the vaccinia virus gene(s) encoding alpha-amanitin resistance (alpha r) (Villarreal et al., J. Virol. 51:359-366, 1984), a collection of alpha r, temperature-sensitive (ts) mutants were isolated. The premise of these experiments was that mutants might be found...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.1.65-70.1986
更新日期:1986-01-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) DNA and RNA have been detected in peripheral nervous system (PNS) and central nervous system (CNS) tissues of latently infected mice. However, explant methods are successful in reactivating HSV-1 only from latently infected PNS tissues. In this report, latent herpesvirus infections ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.3.749-756.1988
更新日期:1988-03-01 00:00:00
abstract::The plus strand of virion DNA of duck hepatitis B virus possessed, at its 5' terminus, a capped oligoribonucleotide 18 to 19 bases in length. This oligoribonucleotide had a unique 5' end, the heterogeneity in length reflecting two distinct junctions with plus-strand DNA that were 1 base apart. The sequence of the RNA ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.1.229-236.1986
更新日期:1986-01-01 00:00:00
abstract::The varicella-zoster virus major transactivator, IE62, contains a potent N-terminal acidic transcriptional activation domain (TAD). Our experiments revealed that the minimal IE62 TAD encompasses amino acids (aa) 19 to 67. We showed that the minimal TAD interacts with the human Mediator complex. Site-specific mutations...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00054-09
更新日期:2009-06-01 00:00:00
abstract::Today, global attention is focused on two influenza virus strains: the current pandemic strain, swine origin influenza virus (H1N1-2009), and the highly pathogenic avian influenza virus, H5N1. At present, the infection caused by the H1N1-2009 is moderate, with mortality rates of less <1%. In contrast, infection with t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02289-09
更新日期:2010-04-01 00:00:00
abstract::Most current diagnostic tests for transmissible spongiform encephalopathies (TSE) rely on the presence of proteinase K (PK)-resistant PrP(Sc) (PrP-res) in postmortem tissues as an indication of TSE disease. However, a number of studies have highlighted a discrepancy between TSE infectivity and PrP-res levels in both n...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03469-12
更新日期:2013-05-01 00:00:00
abstract::The natural life cycle of alphaviruses, a group of plus-strand RNA viruses, involves transmission to vertebrate hosts via mosquitoes. Chronic infections are established in mosquitoes (and usually in mosquito cell cultures), but infection of susceptible vertebrate cells typically results in rapid shutoff of host mRNA t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.5.3854-3865.1999
更新日期:1999-05-01 00:00:00
abstract::R325-beta TK+, a herpes simplex virus 1 mutant carrying a 500-base-pair deletion in the alpha 22 gene and the wild-type (beta) thymidine kinase (TK) gene, was previously shown to grow efficiently in HEp-2 and Vero cell lines. We report that in rodent cell lines exemplified by the Rat-1 line, plating efficiency was red...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.55.2.338-346.1985
更新日期:1985-08-01 00:00:00
abstract::It has been shown that not all but most of the avian influenza viruses replicate in the upper respiratory tract of pigs (H. Kida et al., J. Gen. Virol. 75:2183-2188, 1994). It was shown that A/chicken/Yamaguchi/7/2004 (H5N1) [Ck/Yamaguchi/04 (H5N1)] did not replicate in pigs (N. Isoda et al., Arch. Virol. 151:1267-127...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01879-08
更新日期:2009-02-01 00:00:00
abstract::A recombinant vaccinia virus genome was constructed in which the viral thymidine kinase (tk) gene was placed between direct repeats of a 1.5-kilobase-pair DNA sequence of heterologous origin. When forced to replicate in tk- cells in the presence of methotrexate (i.e., under tk+-selective conditions), the recombinant m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.6.1788-1795.1987
更新日期:1987-06-01 00:00:00
abstract::Luteoviruses and the luteovirus-like pea enation mosaic virus (PEMV; genus Enamovirus) are transmitted by aphids in a circulative, nonreplicative manner. Acquired virus particles persist for several weeks in the aphid hemolymph, in which a GroEL homolog, produced by the primary endosymbiont of the aphid, is abundantly...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.10.7258-7265.1997
更新日期:1997-10-01 00:00:00
abstract::Massive infection of memory CD4 T cells is a hallmark of early simian immunodeficiency virus (SIV) infection, with viral infection peaking at day 10 postinfection (p.i.), when a majority of memory CD4 T cells in mucosal and peripheral tissues are infected. It is not clear if mononuclear cells from the monocyte and mac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06372-11
更新日期:2012-01-01 00:00:00