Abstract:
:The contribution of the hepatitis B virus enhancers I and II in the regulation of the activity of the core and the X promoters was assessed in transgenic mice. Surprisingly, despite the presence of heterologous promoters linked 5' of the X gene, the transgene expression is mostly due to core promoter (Cp) activity present in the X coding sequence. Moreover, the restriction of Cp activity to hepatic tissue required the combined action of both enhancers I and II, whereas the proximity of these two enhancers was insufficient to confer tissue specificity on Xp activity. Furthermore, the liver-specific activity of the Cp was developmentally regulated in an enhancer I-independent manner.
journal_name
J Viroljournal_title
Journal of virologyauthors
Billet O,Grimber G,Levrero M,Seye KA,Briand P,Joulin Vdoi
10.1128/JVI.69.9.5912-5916.1995subject
Has Abstractpub_date
1995-09-01 00:00:00pages
5912-6issue
9eissn
0022-538Xissn
1098-5514journal_volume
69pub_type
杂志文章abstract::The human T-cell leukemia virus type 1 (HTLV-1) transforming protein, Tax, is a potent transactivator of both viral and cellular gene expression. The ability of Tax to transform cells is believed to depend on its transactivation of cellular-growth-regulatory genes. Expression of proliferating cell nuclear antigen (PCN...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.2.1181-1190.1997
更新日期:1997-02-01 00:00:00
abstract::Initiation of the Epstein-Barr virus (EBV) lytic cycle is dependent on expression of the viral transactivator Zta, which is encoded by the BZLF1 gene. Described here is an initial mapping of the regions of Zta involved in activating transcription. The data indicate that the amino-terminal 153 amino acids of Zta are im...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.2.922-929.1992
更新日期:1992-02-01 00:00:00
abstract::Deoxyribonucleic acid (DNA) synthesis was studied in poxvirus-infected cells by measuring (14)C-thymidine incorporation into viral and host cell DNA. A complete separation of the two species of DNA was achieved by combining the previously used "Dounce method" with a separation method based on different reannealing pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.5.401-408.1968
更新日期:1968-05-01 00:00:00
abstract::All known DNA tumor viruses are known to target and inactivate two main cell cycle regulatory proteins, retinoblastoma protein (pRb) and p53. Inactivation of pRb promotes host cell cycle progression into S phase, and inactivation of p53 promotes cell immortalization. The DNA tumor virus Kaposi's sarcoma associated her...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.19.10336-10347.2004
更新日期:2004-10-01 00:00:00
abstract::Marburg virus (MARV) has been associated with sporadic episodes of hemorrhagic fever, including a recent highly publicized outbreak in Angola that produced severe disease and significant mortality in infected patients. MARV is also considered to have potential as a biological weapon. Recently, we reported the developm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00959-06
更新日期:2006-10-01 00:00:00
abstract::The herpesvirus saimiri genome encodes a complement control protein homolog (CCPH). Stable mammalian cell transfectants expressing a recombinant transmembrane form of CCPH (mCCPH) or a 5'FLAG epitope-tagged mCCPH (5'FLAGmCCPH) conferred resistance to complement-mediated cell damage by inhibiting the lytic activity of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.6.3889-3892.1995
更新日期:1995-06-01 00:00:00
abstract::Plasmid lambdadv1, which is in a dimeric form, was converted to a linear monomer duplex by the action of EcoRI restriction endonuclease that incises at a unique site in this plasmid genome. The resulting products were then joined by Escherichia coli DNA ligase to produce molecules with various oligomeric forms, and fr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.3.479-485.1975
更新日期:1975-09-01 00:00:00
abstract::During lytic infection, herpes simplex virus subverts the host cell RNA polymerase II transcription machinery to efficiently express its own genome while repressing the expression of most cellular genes. The mechanism by which RNA polymerase II is directed to the viral delayed-early and late genes is still unresolved....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.988-1001.1994
更新日期:1994-02-01 00:00:00
abstract::Phenol-extracted, infectious deoxyribonucleic acid (DNA) species from phi105 phage particles, from phi105 lysogenic bacteria, and from induced phi105 lysogenic bacteria were sedimented in sucrose gradients. Infectious DNA from phi105 particles sedimented like the bulk of mature phage DNA in neutral sucrose. Infectivit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.5.5.604-608.1970
更新日期:1970-05-01 00:00:00
abstract::Purified preparations of human adenovirus type 12 showed two bands when subjected to isopycnic centrifugation in a density gradient of cesium chloride. Their density difference was about 0.003 g/ml, suggesting a small difference in their deoxyribonucleic acid to protein ratio. Virions with a lighter density can kill h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.4.426-433.1971
更新日期:1971-04-01 00:00:00
abstract::Antigen-presenting cells are a heterogeneous group of cells that are characterized by their functional specialization. Consequently, targeting specific antigen-presenting cell subsets offers opportunities to induce distinct T cell responses. Here we report on the generation and use of nanobodies (Nbs) to target lentiv...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01498-13
更新日期:2013-10-01 00:00:00
abstract:UNLABELLED:HIV-1 Vpu decreases the exposure of epitopes within the viral envelope glycoprotein (Env) on the surface of infected cells by downregulating both BST2 and CD4. To test the hypothesis that inhibiting Vpu activity would increase the exposure of these epitopes and sensitize infected cells to antibody-dependent ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02736-15
更新日期:2015-12-16 00:00:00
abstract::The investigation of papillomavirus chromatin has been hampered by the unavailability of a tissue culture system for vegetative growth of these viruses. We have used, therefore, bovine papillomavirus type 1-transformed hamster embryo fibroblasts containing 200 to 250 episomal genome equivalents per cell as a source of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.2.567-574.1983
更新日期:1983-05-01 00:00:00
abstract::Adenovirus type 7 (Ad7) early region 1 mRNA species transcribed in rat cell lines transformed by the HindIII-I . J fragment (the left 7.8% of the viral genome) and in human KB cells infected with Ad7 were mapped on the viral genome, using S1 nuclease gel and diazobenzyloxymethyl paper hybridization techniques. At the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.2.337-352.1980
更新日期:1980-11-01 00:00:00
abstract::The putative envelope 2 (E2) gene of hepatitis C virus (HCV) contains a highly variable region referred to as hypervariable region 1 (HVR1). We hypothesized that this genetic variability is driven by immune selection pressure, rather than representing the accumulation of random mutations in a region with relatively li...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.7.3058-3066.2000
更新日期:2000-04-01 00:00:00
abstract::Influenza pandemic preparedness has focused on influenza virus H5 and H7 subtypes. However, it is not possible to predict with certainty which subtype of avian influenza virus will cause the next pandemic, and it is prudent to include other avian influenza virus subtypes in pandemic preparedness efforts. An H6 influen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01206-08
更新日期:2008-11-01 00:00:00
abstract:UNLABELLED:Noroviruses are the leading cause of acute gastroenteritis outbreaks worldwide. The majority of norovirus outbreaks are caused by genogroup II.4 (GII.4). Novel GII.4 strains emerge every 2 to 4 years and replace older variants as the dominant norovirus. Novel variants emerge through a combination of recombin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02494-14
更新日期:2014-12-01 00:00:00
abstract::We previously identified two novel endogenous murine leukemia virus proviruses, PreXMRV-1 and PreXMRV-2, and showed that they most likely recombined during xenograft passaging of a human prostate tumor in mice to generate xenotropic murine leukemia virus-related virus (XMRV). To determine the recombination potential o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01787-13
更新日期:2013-11-01 00:00:00
abstract::Viral attenuation may be due to lowered efficiency of certain steps essential for viral multiplication. For the construction of less neurovirulent strains of poliovirus in vitro, we introduced deletions into the 5' noncoding sequence (742 nucleotides long) of the genomes of the Mahoney and Sabin 1 strains of polioviru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.12.5354-5363.1989
更新日期:1989-12-01 00:00:00
abstract::In the immunocompetent host, primary cytomegalovirus (CMV) infection is resolved by the immune response without causing overt disease. The viral genome, however, is not cleared but is maintained in a latent state that entails a risk of virus recurrence and consequent organ disease. By using murine CMV as a model, we h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.1797-1804.1998
更新日期:1998-03-01 00:00:00
abstract::We have identified a novel brain-derived single-stranded-DNA-binding protein that interacts with a region of the human neurotropic JC virus enhancer designated the lytic control element (LCE). This nuclear factor, LCP-1 (for lytic control element-binding protein 1), specifically recognizes the LCE, as determined by ge...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.12.6885-6892.1992
更新日期:1992-12-01 00:00:00
abstract::Previous studies with simian virus 40-transformed mouse 3T3 cells which are temperature sensitive for the expression of the transformed phenotype (ts SV3T3 cells) have shown that T-antigen expression and viral DNA transcription are under cell cycle control. Using these ts SV3T3 cells, we studied the expression of the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.30.3.711-719.1979
更新日期:1979-06-01 00:00:00
abstract::Bovine herpesvirus 1 (BoHV-1) is an alphaherpesvirus that causes disease in cattle populations worldwide. Sphingomyelin (SM) is the most abundant sphingolipid in the mammalian cell membrane, where it preferentially associates with cholesterol to form lipid raft domains. SM is a substrate for the lysosome-resident enzy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01547-18
更新日期:2019-02-19 00:00:00
abstract::The accumulation of an abnormal, protease-resistant form of the protein PrP (PrP-res) in hosts with scrapie and related transmissible spongiform encephalopathies appears to be important in disease pathogenesis. To gain insight into the mechanism of PrP-res accumulation and the in vivo antiscrapie activity of certain p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.2.643-650.1993
更新日期:1993-02-01 00:00:00
abstract::Four new nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid viruses have been isolated. Although these viruses (designated Ad2(+)ND(2), Ad2(+)ND(3), Ad2(+)ND(4), and Ad2(+)ND(5)) were clonal derivatives of the same Ad2-SV40 hybrid population, they differ significantly from each other and from the previously...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.11.5.655-664.1973
更新日期:1973-05-01 00:00:00
abstract::The infection of human fetal thymus organ cultures (FTOC) with coxsackievirus B4 E2 (CVB4 E2) was investigated. Both positive- and negative-strand viral RNA were detected by real-time quantitative reverse transcription-PCR (RT-PCR) in CVB4 E2-infected FTOC, which supported high yields of virus production (approximatel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.18.9854-9861.2004
更新日期:2004-09-01 00:00:00
abstract::Multimerization of the Hantaan virus nucleocapsid protein (NP) in Hantaan virus-infected Vero E6 cells was observed in a competitive enzyme-linked immunosorbent assay (ELISA). Recombinant and truncated NPs of Hantaan, Seoul, and Dobrava viruses lacking the N-terminal 49 amino acids were also detected as multimers. Alt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.2.943-952.2003
更新日期:2003-01-01 00:00:00
abstract::Ribosomes from ghost-treated Escherichia coli can form in vitro initiation complexes but cannot translate added template RNA because of a defect in the 30S subunit; 16S rRNA is degraded. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.5.1310-1313.1974
更新日期:1974-11-01 00:00:00
abstract::Second-site revertants from replication-incompetent molecular clones of human immunodeficiency virus (HIV) contain base substitutions adjacent to the TATA motif. The altered TATA box motifs were analyzed for their effect(s) on virus infectivity, long terminal repeat (LTR)-directed expression in transient transfection ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.5.3298-3307.1994
更新日期:1994-05-01 00:00:00
abstract:UNLABELLED:Numerous experimental studies have demonstrated that CD8(+) T cells contribute to immunity against influenza by limiting viral replication. It is therefore surprising that rigorous statistical tests have failed to find evidence of positive selection in the epitopes targeted by CD8(+) T cells. Here we use a n...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01571-15
更新日期:2015-11-01 00:00:00