当前位置： SCI文献检索 > JOURNAL OF VIROLOGY期刊下所有文献 > Construction of less neurovirulent polioviruses by introducing deletions into the 5' noncoding sequence of the genome.

Construction of less neurovirulent polioviruses by introducing deletions into the 5' noncoding sequence of the genome.

Abstract：

:Viral attenuation may be due to lowered efficiency of certain steps essential for viral multiplication. For the construction of less neurovirulent strains of poliovirus in vitro, we introduced deletions into the 5' noncoding sequence (742 nucleotides long) of the genomes of the Mahoney and Sabin 1 strains of poliovirus type 1 by using infectious cDNA clones of the virus strains. Plaque sizes shown by deletion mutants were used as a marker for rate of viral proliferation. Deletion mutants of both the strains thus constructed lacked a genome region of nucleotide positions 564 to 726. The sizes of plaques displayed by these deletion mutants were smaller than those by the respective parental viruses, although a phenotype referring to reproductive capacity at different temperatures (rct) of viruses was not affected by introduction of the deletion. Monkey neurovirulence tests were performed on the deletion mutants. The results clearly indicated that the deletion mutants had much less neurovirulence than with the corresponding parent viruses. Production of infectious particles and virus-specific protein synthesis in cells infected with the deletion mutants started later than in those infected with the parental viruses. The rate at which cytopathic effect progressed was also slower in cells infected with the mutants. Phenotypic stability of the deletion mutant for small-plaque phenotype and temperature sensitivity was investigated after passaging the mutant at an elevated temperature of 37.5 degrees C. Our data strongly suggested that the less neurovirulent phenotype introduced by the deletion is very stable during passaging of the virus.

journal_name

J Virol

journal_title

Journal of virology

authors

Iizuka N,Kohara M,Hagino-Yamagishi K,Abe S,Komatsu T,Tago K,Arita M,Nomoto A

doi

10.1128/JVI.63.12.5354-5363.1989

subject

Has Abstract

pub_date

1989-12-01 00:00:00

pages

5354-63

issue

12

eissn

0022-538X

issn

1098-5514

journal_volume

63

pub_type

杂志文章

相关文献

JOURNAL OF VIROLOGY文献大全
  • T-cell infiltration correlates with CXCL10 expression in ganglia of cynomolgus macaques with reactivated simian varicella virus.

    abstract：:Ganglia of monkeys with reactivated simian varicella virus (SVV) contained more CD8 than CD4 T cells around neurons. The abundance of CD8 T cells was greater less than 2 months after reactivation than that at later times and correlated with that of CXCL10 RNA but not with those of SVV protein or open reading frame 61 ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.03181-12

    authors： Ouwendijk WJ,Abendroth A,Traina-Dorge V,Getu S,Steain M,Wellish M,Andeweg AC,Osterhaus AD,Gilden D,Verjans GM,Mahalingam R

    更新日期：2013-03-01 00:00:00

  • Key molecular factors in hemagglutinin and PB2 contribute to efficient transmission of the 2009 H1N1 pandemic influenza virus.

    abstract：:Animal influenza viruses pose a clear threat to public health. Transmissibility among humans is a prerequisite for a novel influenza virus to cause a human pandemic. A novel reassortant swine influenza virus acquired sustained human-to-human transmissibility and caused the 2009 influenza pandemic. However, the molecul...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.00958-12

    authors： Zhang Y,Zhang Q,Gao Y,He X,Kong H,Jiang Y,Guan Y,Xia X,Shu Y,Kawaoka Y,Bu Z,Chen H

    更新日期：2012-09-01 00:00:00

  • Herpes simplex virus type 2 glycoprotein G-negative clinical isolates are generated by single frameshift mutations.

    abstract：:Herpes simplex virus (HSV) codes for several envelope glycoproteins, including glycoprotein G-2 (gG-2) of HSV type 2 (HSV-2), which are dispensable for replication in cell culture. However, clinical isolates which are deficient in such proteins occur rarely. We describe here five clinical HSV-2 isolates which were fou...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.73.12.9796-9802.1999

    authors： Liljeqvist JA,Svennerholm B,Bergström T

    更新日期：1999-12-01 00:00:00

  • High-level transcription from the adenovirus major late promoter requires downstream binding sites for late-phase-specific factors.

    abstract：:The adenovirus major late promoter (MLP) is active during both the early and late phases of infection. During the early phase the activity of the MLP is similar to those of the other early viral promoters, but during the late phase the rate of transcription from the MLP becomes much greater by comparison. We report he...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.64.1.51-60.1990

    authors： Leong K,Lee W,Berk AJ

    更新日期：1990-01-01 00:00:00

  • Emergence of simian virus 40 variants during serial passage of plaque isolates.

    abstract：:Three serial passage series of simian virus 40 (SV40) in CV-1 cells were initiated by infection directly from the same wild-type plaque isolate, three series were initiated by infection with another plaque isolate, and two series were initiated with each of two other plaque isolates. Aberrant SV40 genomes were not det...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.42.2.730-733.1982

    authors： Norkin LC,Tirrell SM

    更新日期：1982-05-01 00:00:00

  • Genetic location of a mutant of bacteriophage T4 deficient in the ability to induce endonuclease II.

    abstract：:Reciprocal three-factor crosses and the use of a partial revertant of a putative ribonucleotide reductase mutant of Escherichia coli B/5 as indicator have made it possible to map denA (deficient in endonuclease II) between nrd-11 (ribonucleotide reductase gene B) and amM69 (gene 63) on the bacteriophage T4 chromosome....

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.9.1.184-186.1972

    authors： Ray P,Sinha NK,Warner HR,Snustad DP

    更新日期：1972-01-01 00:00:00

  • Synergistic roles of antibody and interferon in noncytolytic clearance of Sindbis virus from different regions of the central nervous system.

    abstract：:Sindbis virus (SINV) is an alphavirus that causes infection of neurons and encephalomyelitis in adult immunocompetent mice. Recovery can occur without apparent neurological damage. To better define the factors facilitating noncytolytic clearance of SINV in different regions of the central nervous system (CNS) and the ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.01152-06

    authors： Burdeinick-Kerr R,Wind J,Griffin DE

    更新日期：2007-06-01 00:00:00

  • Lentiviral vector-based prime/boost vaccination against AIDS: pilot study shows protection against Simian immunodeficiency virus SIVmac251 challenge in macaques.

    abstract：:AIDS vaccination has a pressing need for more potent vaccination vectors capable of eliciting strong, diversified, and long-lasting cellular immune responses against human immunodeficiency virus (HIV). Lentiviral vectors have demonstrated efficiency not only as gene delivery vehicles for gene therapy applications but ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.01284-09

    authors： Beignon AS,Mollier K,Liard C,Coutant F,Munier S,Rivière J,Souque P,Charneau P

    更新日期：2009-11-01 00:00:00

  • VP2 cleavage and the leucine ring at the base of the fivefold cylinder control pH-dependent externalization of both the VP1 N terminus and the genome of minute virus of mice.

    abstract：:Cylindrical projections surrounding the fivefold-symmetry axes in minute virus of mice (MVM) harbor central pores that penetrate through the virion shell. In newly released DNA-containing particles, these pores contain residues 28 to 38 belonging to a single copy of VP2, disposed so that its extreme N-terminal domain ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.80.1.161-171.2006

    authors： Farr GA,Cotmore SF,Tattersall P

    更新日期：2006-01-01 00:00:00

  • Rorgamma (Rorc) is a common integration site in type B leukemogenic virus-induced T-cell lymphomas.

    abstract：:The retrovirus type B leukemogenic virus (TBLV) causes T-cell lymphomas in mice. We have identified the Rorgamma locus as an integration site in 19% of TBLV-induced tumors. Overexpression of one or more Rorgamma isoforms in >77% of the tumors tested may complement apoptotic effects of c-myc overexpression. ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/jvi.78.9.4943-4946.2004

    authors： Broussard DR,Lozano MM,Dudley JP

    更新日期：2004-05-01 00:00:00

  • Resistance mutations in human immunodeficiency virus type 1 integrase selected with elvitegravir confer reduced susceptibility to a wide range of integrase inhibitors.

    abstract：:Integration of viral DNA into the host chromosome is an essential step in the life cycle of retroviruses and is facilitated by the viral integrase enzyme. The first generation of integrase inhibitors recently approved or currently in late-stage clinical trials shows great promise for the treatment of human immunodefic...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.00470-08

    authors： Goethals O,Clayton R,Van Ginderen M,Vereycken I,Wagemans E,Geluykens P,Dockx K,Strijbos R,Smits V,Vos A,Meersseman G,Jochmans D,Vermeire K,Schols D,Hallenberger S,Hertogs K

    更新日期：2008-11-01 00:00:00

  • Nucleotide sequence analysis of the long terminal repeat of integrated simian sarcoma virus: evolutionary relationship with other mammalian retroviral long terminal repeats.

    abstract：:Nucleotide sequence analysis of the long terminal repeat (LTR) of the integrated simian sarcoma virus showed that the simian sarcoma virus LTR comprised 504 nucleotides with an inverted repeat of seven bases at its 5' and 3' termini. At the site of simian sarcoma virus integration, cellular flanking sequences adjacent...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.42.3.1108-1113.1982

    authors： Devare SG,Reddy EP,Law JD,Aaronson SA

    更新日期：1982-06-01 00:00:00

  • The PA-gene-mediated lethal dissemination and excessive innate immune response contribute to the high virulence of H5N1 avian influenza virus in mice.

    abstract：:Highly pathogenic H5N1 influenza A virus remains a substantial threat to public health. To understand the molecular basis and host mechanism for the high virulence of H5N1 viruses in mammals, we compared two H5N1 isolates which have similar genetic backgrounds but greatly differ in their virulence in mice. A/Chicken/J...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.02891-12

    authors： Hu J,Hu Z,Song Q,Gu M,Liu X,Wang X,Hu S,Chen C,Liu H,Liu W,Chen S,Peng D,Liu X

    更新日期：2013-03-01 00:00:00

  • In Vivo Dynamics of Reporter Flaviviridae Viruses.

    abstract：:Recombinant viruses possessing reporter proteins have been generated for virus research. In the case of the family Flaviviridae, we recently generated recombinant viruses, including the hepatitis C virus of the genus Hepacivirus, Japanese encephalitis virus (JEV) of the genus Flavivirus, and bovine viral diarrhea viru...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.01191-19

    authors： Tamura T,Igarashi M,Enkhbold B,Suzuki T,Okamatsu M,Ono C,Mori H,Izumi T,Sato A,Fauzyah Y,Okamoto T,Sakoda Y,Fukuhara T,Matsuura Y

    更新日期：2019-10-29 00:00:00

  • CXC chemokine ligand 10 controls viral infection in the central nervous system: evidence for a role in innate immune response through recruitment and activation of natural killer cells.

    abstract：:How chemokines shape the immune response to viral infection of the central nervous system (CNS) has largely been considered within the context of recruitment and activation of antigen-specific lymphocytes. However, chemokines are expressed early following viral infection, suggesting an important role in coordinating i...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/jvi.78.2.585-594.2004

    authors： Trifilo MJ,Montalto-Morrison C,Stiles LN,Hurst KR,Hardison JL,Manning JE,Masters PS,Lane TE

    更新日期：2004-01-01 00:00:00

  • Hepatitis Delta Virus Alters the Autophagy Process To Promote Its Genome Replication.

    abstract：:A substantial number of viruses have been demonstrated to subvert autophagy to promote their own replication. Recent publications have reported the proviral effect of autophagy induction on hepatitis B virus (HBV) replication. Hepatitis delta virus (HDV) is a defective virus and an occasional obligate satellite of HBV...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.01936-19

    authors： Khabir M,Aliche AZ,Sureau C,Blanchet M,Labonté P

    更新日期：2020-01-31 00:00:00

  • Attenuated murine cytomegalovirus binds to N-acetylglucosamine, and shift to virulence may involve recognition of sialic acids.

    abstract：:Treatment of cells with lectins specific for N-acetylglucosamine (GlcNAc) blocked infection by mouse cytomegalovirus (MCMV), and GlcNAc pretreatment of the lectin blocked this effect. MCMV failed to infect N-acetylglucosaminidase (GlcNAcase)-treated mouse embryo fibroblasts (MEF). GlcNAc and GlcNAc-containing syntheti...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.64.11.5430-5440.1990

    authors： Ravindranath RM,Graves MC

    更新日期：1990-11-01 00:00:00

  • Transcriptional activities of mammalian genomes at sites of recombination with foreign DNA.

    abstract：:The nucleotide sequences of several sites of recombination between adenovirus DNA and hamster, mouse, or human cell DNAs were determined. These sites of recombination had been cloned from adenovirus type 2 (Ad2)- or type 12 (Ad12)-transformed cells, from Ad12-induced tumor cells, or from a symmetric recombinant betwee...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.61.2.344-353.1987

    authors： Schulz M,Freisem-Rabien U,Jessberger R,Doerfler W

    更新日期：1987-02-01 00:00:00

  • Immunogenicity of novel mumps vaccine candidates generated by genetic modification.

    abstract：:Mumps is a highly contagious human disease, characterized by lateral or bilateral nonsuppurative swelling of the parotid glands and neurological complications that can result in aseptic meningitis or encephalitis. A mumps vaccination program implemented since the 1960s reduced mumps incidence by more than 99% and kept...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.02778-13

    authors： Xu P,Chen Z,Phan S,Pickar A,He B

    更新日期：2014-03-01 00:00:00

  • Late domain function identified in the vesicular stomatitis virus M protein by use of rhabdovirus-retrovirus chimeras.

    abstract：:Little is known about the mechanisms used by enveloped viruses to separate themselves from the cell surface at the final step of budding. However, small sequences in the Gag proteins of several retroviruses (L domains) have been implicated in this process. A sequence has been identified in the M proteins of rhabdoviru...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.73.4.3359-3365.1999

    authors： Craven RC,Harty RN,Paragas J,Palese P,Wills JW

    更新日期：1999-04-01 00:00:00

  • A Genome-Wide Epstein-Barr Virus Polyadenylation Map and Its Antisense RNA to EBNA.

    abstract：:Epstein-Barr virus (EBV) is a ubiquitous human pathogen associated with Burkitt's lymphoma and nasopharyngeal carcinoma. Although the EBV genome harbors more than a hundred genes, a full transcription map with EBV polyadenylation profiles remains unknown. To elucidate the 3' ends of all EBV transcripts genome-wide, we...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.01593-18

    authors： Majerciak V,Yang W,Zheng J,Zhu J,Zheng ZM

    更新日期：2019-01-04 00:00:00

  • Human plasma enhances the infectivity of primary human immunodeficiency virus type 1 isolates in peripheral blood mononuclear cells and monocyte-derived macrophages.

    abstract：:Physiological microenvironments such as blood, seminal plasma, mucosal secretions, or lymphatic fluids may influence the biology of the virus-host cell and immune interactions for human immunodeficiency virus type 1 (HIV-1). Relative to media, physiological levels of human plasma were found to enhance the infectivity ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.69.10.6054-6062.1995

    authors： Wu SC,Spouge JL,Conley SR,Tsai WP,Merges MJ,Nara PL

    更新日期：1995-10-01 00:00:00

  • RNA polymerase II holoenzyme modifications accompany transcription reprogramming in herpes simplex virus type 1-infected cells.

    abstract：:During lytic infection, herpes simplex virus type 1 (HSV-1) represses host transcription, recruits RNA polymerase II (RNAP II) to viral replication compartments, and alters the phosphorylation state of the RNAP II large subunit. Host transcription repression and RNAP II modifications require expression of viral immedi...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.75.20.9872-9884.2001

    authors： Jenkins HL,Spencer CA

    更新日期：2001-10-01 00:00:00

  • Bronchoalveolar fluid is not a major hindrance to virus-mediated gene therapy in cystic fibrosis.

    abstract：:Successfully targeting the airway epithelium is essential for gene therapy of some pulmonary diseases. However, the airway epithelium is resistant to virus-mediated gene transfer with commonly used vectors. Vectors that interact with endogenously expressed receptors on the apical surface significantly increase gene tr...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/jvi.76.20.10437-10443.2002

    authors： Rooney CP,Denning GM,Davis BP,Flaherty DM,Chiorini JA,Zabner J

    更新日期：2002-10-01 00:00:00

  • Chromosomal clustering of five defined endogenous retrovirus loci in White Leghorn chickens.

    abstract：:By using in situ hybridization, we localized three endogenous chicken retrovirus loci associated with the gs- chf- phenotype to distinct regions on chromosome 1. These three loci, ev4, ev5, and ev8, along with the two other known gs- chf--associated loci in White Leghorn chickens, ev1 and ev13, appear to be evenly dis...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.43.2.737-740.1982

    authors： Tereba A,Astrin SM

    更新日期：1982-08-01 00:00:00

  • Recombinant newcastle disease virus expressing a foreign viral antigen is attenuated and highly immunogenic in primates.

    abstract：:Paramyxoviruses such as human parainfluenza viruses that bear inserts encoding protective antigens of heterologous viruses can induce an effective immunity against the heterologous viruses in experimental animals. However, vectors based on common human pathogens would be expected to be restricted in replication in the...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.79.21.13275-13284.2005

    authors： Bukreyev A,Huang Z,Yang L,Elankumaran S,St Claire M,Murphy BR,Samal SK,Collins PL

    更新日期：2005-11-01 00:00:00

  • Rotavirus entry: a deep journey into the cell with several exits.

    abstract：:Rotaviruses are the leading etiological agents of acute gastroenteritis in infants and young children worldwide. These nonenveloped viruses enter cells using different types of endocytosis and, depending on the virus strain, travel to different endosomal compartments before exiting to the cytosolic space. In this Gem,...

    journal_title：Journal of virology

    pub_type： 杂志文章,评审

    doi：10.1128/JVI.01787-14

    authors： Arias CF,Silva-Ayala D,López S

    更新日期：2015-01-15 00:00:00

  • Antiviral activity of polyacrylic and polymethacrylic acids. II. Mode of action in vivo.

    abstract：:A marked virus-inhibiting potency is obtained in the serum after intraperitoneal injection of polyacrylic acid (PAA) and polymethacrylic acid (PMAA) in mice. Much higher antiviral levels were reached than for other related polymers including dextran sulfate, heparin, polyvinyl sulfate, pyran copolymer, polystyrene sul...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.2.9.886-893.1968

    authors： De Somer P,De Clercq E,Billiau A,Schonne E,Claesen M

    更新日期：1968-09-01 00:00:00

  • The small envelope glycoprotein (GS) of equine arteritis virus folds into three distinct monomers and a disulfide-linked dimer.

    abstract：:The small membrane glycoprotein (GS) of equine arteritis virus (EAV) is a minor virion component but is abundantly expressed in EAV-infected cells. In this study, we have analyzed its membrane topology, folding, oligomerization, and intracellular transport. We show that GS is a class I integral membrane protein with o...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.69.6.3441-3448.1995

    authors： de Vries AA,Raamsman MJ,van Dijk HA,Horzinek MC,Rottier PJ

    更新日期：1995-06-01 00:00:00

  • Impact of HLA-B*52:01-Driven Escape Mutations on Viral Replicative Capacity.

    abstract：:HLA-B*52:01 is strongly associated with protection against HIV disease progression. However, the mechanisms of HLA-B*52:01-mediated immune control have not been well studied. We here describe a cohort with a majority of HIV C-clade-infected individuals from Delhi, India, where HLA-B*52:01 is highly prevalent (phenotyp...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.02025-19

    authors： Tsai MC,Singh S,Adland E,Goulder P

    更新日期：2020-06-16 00:00:00