Abstract:
:Little is known about the mechanisms used by enveloped viruses to separate themselves from the cell surface at the final step of budding. However, small sequences in the Gag proteins of several retroviruses (L domains) have been implicated in this process. A sequence has been identified in the M proteins of rhabdoviruses that closely resembles the PPPPY motif in the L domain of Rous sarcoma virus (RSV), an avian retrovirus. To evaluate whether the PPPY sequence in vesicular stomatitis virus (VSV) M protein has an activity analogous to that of the retroviral sequence, M-Gag chimeras were characterized. The N-terminal 74 amino acids of the VSV (Indiana) M protein, including the PPPY motif, was able to replace the L domain of RSV Gag and allow the assembly and release of virus-like particles. Alanine substitutions in the VSV PPPY motif severely compromised the budding activity of this hybrid protein but not that of another chimera which also contained the RSV PPPPY sequence. We conclude that this VSV sequence is functionally homologous to the RSV L domain in promoting virus particle release, making this the first example of such an activity in a virus other than a retrovirus. Both the RSV and VSV motifs have been shown to interact in vitro with certain cellular proteins that contain a WW interaction module, suggesting that the L domains are sites of interaction with unknown host machinery involved in virus release.
journal_name
J Viroljournal_title
Journal of virologyauthors
Craven RC,Harty RN,Paragas J,Palese P,Wills JWdoi
10.1128/JVI.73.4.3359-3365.1999subject
Has Abstractpub_date
1999-04-01 00:00:00pages
3359-65issue
4eissn
0022-538Xissn
1098-5514journal_volume
73pub_type
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pub_type: 杂志文章
doi:10.1128/JVI.21.1.131-138.1977
更新日期:1977-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-02-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1994-06-01 00:00:00
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2006-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2001-11-01 00:00:00