Abstract:
:The nature and theory of flow field-flow fractionation is described, and its potential applicability to virus-like particles is discussed. Different virus types are shown to be retained at different levels. Retention can be controlled by variation of the experimental parameters, in good agreement with theory. However, a mild adsorption effect is indicated and requires the development of alternate strategies for measuring diffusion coefficients. For Qbeta, our value agrees well within 10% of literature values; the values obtained for other viruses, using Abeta as an internal standard, are untested. Finally, it is demonstrated that flow field-flow fractionation can cleanly fractionate two viruses from one another and from an albumin impurity, that samples as large as several milligrams in size can be analyzed, and that the method has potential utility in the quantitative and qualitative analysis of virus systems.
journal_name
J Viroljournal_title
Journal of virologyauthors
Giddings JC,Yang FJ,Myers MNdoi
10.1128/JVI.21.1.131-138.1977subject
Has Abstractpub_date
1977-01-01 00:00:00pages
131-8issue
1eissn
0022-538Xissn
1098-5514journal_volume
21pub_type
杂志文章abstract::Recent studies have explored the chromatin structures associated with the herpes simplex virus type 1 (HSV-1) genome during latency, particularly with regard to specific histone tail modifications such as acetylation and dimethylation. The objective of our present study was to develop a rapid systemic method of in viv...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00070-07
更新日期:2007-06-01 00:00:00
abstract::Hepatitis delta virus (HDV) contains two types of hepatitis delta antigens (HDAg) in the virion. The small form (S-HDAg) is required for HDV RNA replication, whereas the large form (L-HDAg) potently inhibits it by a dominant-negative inhibitory mechanism. The sequential appearance of these two forms in the infected ce...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.16.7375-7380.2000
更新日期:2000-08-01 00:00:00
abstract:UNLABELLED:Capsid-associated tegument proteins have been identified in alpha- and betaherpesviruses to play an essential role in viral DNA packaging. Whether and how such tegument proteins exist in gammaherpesviruses have been mysteries. Here, we report a 6-Å-resolution cryo-electron microscopy (cryo-EM) structure of K...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01509-14
更新日期:2014-11-01 00:00:00
abstract::The bovine papillomavirus type 1 (BPV-1) genome replicates as a multiple-copy plasmid in murine C127 cells transformed to neoplasia by virus infection or by transfection with BPV-1 DNA. It was reported previously that BPV-1 genomes harboring frameshift mutations in the E6 or E7 open reading frame (ORF) replicated in C...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.8.4957-4965.1992
更新日期:1992-08-01 00:00:00
abstract::Glycoprotein gIII of pseudorabies virus is a major antigen found in the envelopes of virus particles as well as in and on the surfaces of infected cells. It is not an essential gene product for virus growth in tissue culture. In this report, we provide evidence that, although it is not essential, the gIII protein is r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.7.2512-2515.1988
更新日期:1988-07-01 00:00:00
abstract::Virus-specific cytotoxic T lymphocytes (CTL) exert intense selection pressure on replicating simian immunodeficiency virus (SIV) and human immunodeficiency virus type 1 (HIV-1) in infected individuals. The immunodominant Mamu-A(*)01-restricted Gag p11C, C-M epitope is highly conserved among all sequenced isolates of S...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.23.12572-12578.2003
更新日期:2003-12-01 00:00:00
abstract::The pathogenic parvovirus B19 (B19V) has an extreme tropism for human erythroid progenitor cells. In vitro, only a few erythroid leukemic cell lines (JK-1 and KU812Ep6) or megakaryoblastoid cell lines (UT7/Epo and UT7/Epo-S1) with erythroid characteristics support B19V replication, but these cells are only semipermiss...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02247-07
更新日期:2008-03-01 00:00:00
abstract::The abilities to infect and transmit efficiently among humans are essential for a novel influenza A virus to cause a pandemic. To evaluate the pandemic potential of widely disseminated H5N1 influenza viruses, a ferret contact model using experimental groups comprised of one inoculated ferret and two contact ferrets wa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00170-07
更新日期:2007-07-01 00:00:00
abstract::By using an isothermal multiply primed rolling-circle amplification protocol, the complete genomic DNA of a novel papillomavirus was amplified from a skin lesion biopsy of a Florida manatee (Trichechus manatus latirostris), one of the most endangered marine mammals in United States coastal waters. The nucleotide seque...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.22.12698-12702.2004
更新日期:2004-11-01 00:00:00
abstract::Replication of human papillomavirus type 11 (HPV-11) DNA requires the full-length viral E1 and E2 proteins (C.-M. Chiang, M. Ustav, A. Stenlund, T. F. Ho, T. R. Broker, and L. T. Chow, Proc. Natl. Acad. Sci. USA 89:5799-5803, 1992). Using transient transfection of subgenomic HPV DNA into hamster CHO and human 293 cell...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.9.5224-5231.1992
更新日期:1992-09-01 00:00:00
abstract::RNA viruses of filamentous fungi fall into two broad categories, those that contain double-stranded RNA (dsRNA) genomes in rigid particles and those that are more closely related to positive-sense, single-stranded RNA viruses with dsRNA replicative intermediates found within lipid vesicles. Effective infectivity syste...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.2.892-898.2004
更新日期:2004-01-01 00:00:00
abstract::We established a novel experimental model for human T-cell leukemia virus type 1 (HTLV-1)-induced tumor using NOD-SCID/gammac(null) (NOG) mice. This model is very useful for investigating the mechanism of tumorigenesis and malignant cell growth of adult T-cell leukemia (ATL)/lymphoma, which still remains unclear. Nine...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.9.5286-5294.2003
更新日期:2003-05-01 00:00:00
abstract::The structure of Bacillus subtilis bacteriophage phi25 and phi25 deoxyribonucleic acid (DNA) were studied by electron microscopy. The head of phi25 is a regular polyhedron measuring 75 nm in diameter. The uncontracted tail of phi25 is 130 nm in length and includes a large, complex tail plate. Phage phi25 DNA is double...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.6.1.107-113.1970
更新日期:1970-07-01 00:00:00
abstract:UNLABELLED:Hepatitis B virus (HBV) infects hundreds of millions of people worldwide and causes acute and chronic hepatitis, cirrhosis, and hepatocellular carcinoma. HBV is an enveloped virus with a relaxed circular (RC) DNA genome. In the nuclei of infected human hepatocytes, conversion of RC DNA from the incoming viri...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01263-15
更新日期:2015-10-21 00:00:00
abstract::Adult T-cell leukemia (ATL) is a fatal malignancy of CD4+ T cells infected with human T-cell leukemia virus type 1 (HTLV-1). ATL cells often exhibit random gross chromosomal rearrangements that are associated with the induction and improper repair of double-stranded DNA breaks (DSBs). The viral oncoprotein Tax has bee...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00672-18
更新日期:2018-07-17 00:00:00
abstract::Production of Sindbis virus in the presence of transcription and translation inhibitors was examined in three Aedes albopictus cell lines. Addition of cycloheximide to heat-resistant Sindbis virus (SVHR)-infected mosquito cells arrested viral RNA synthesis completely, in contrast to the effects of this drug on virus-i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.8.2629-2635.1988
更新日期:1988-08-01 00:00:00
abstract::Using in situ hybridization, we demonstrated that latent herpes simplex virus type 1 (HSV-1) gene expression is prevalent in human adult nodose ganglia. This suggests that infection of gastrointestinal sensory nerves, probably through swallowed virus-laden oral secretions, occurs commonly and that HSV-1 reactivating f...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.5.4103-4106.1997
更新日期:1997-05-01 00:00:00
abstract::Interleukin-15 (IL-15), natural killer (NK) cells, and NK T (NKT) cells, components of the innate immune system, are known to contribute to defense against pathogens, including viruses. Here we report that IL-15(-/-) (NK(-) and NKT(-/+)) mice and RAG-2(-/-)/gamma(c)(-/-) (NK(-) and NKT(-)) mice that lack all lymphoid ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.18.10168-10171.2003
更新日期:2003-09-01 00:00:00
abstract::Virion capture assays, in which immobilized antibodies (Abs) capture virus particles, have been used to suggest that nonneutralizing Abs bind effectively to human immunodeficiency virus type 1 (HIV-1) primary viruses. Here, we show that virion capture assays, under conditions commonly reported in the literature, give ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.1.353-365.2003
更新日期:2003-01-01 00:00:00
abstract::Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA-2) is a key determinant in the EBV-driven B-cell growth transformation process. By activating an array of viral and cellular target genes, EBNA-2 initiates a cascade of events which ultimately cause cell cycle entry and the proliferation of the infected B cell. In order...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00665-06
更新日期:2006-10-01 00:00:00
abstract::Transcription and replication signals within the negative-sense genomic RNA of vesicular stomatitis virus (VSV) are located at the 3' terminus. To identify these signals, we have used a transcription- and replication-competent minigenome of VSV to generate a series of deletions spanning the first 47 nucleotides at the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.1.444-452.1999
更新日期:1999-01-01 00:00:00
abstract::The insertion of a particular phi X DNA sequence in the plasmid pACYC177 strongly decreased the capacity of Escherichia coli cells containing such a plasmid to propagate bacteriophage phi X174. The smallest DNA sequence tested that showed the effect was the HindII fragment R4. This fragment does not code for a complet...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.1.1-11.1982
更新日期:1982-04-01 00:00:00
abstract::Immunosuppressed patients are at risk for developing Epstein-Barr Virus (EBV)-positive lymphomas that express the major EBV oncoprotein, LMP1. Although increasing evidence suggests that a small number of lytically infected cells may promote EBV-positive lymphomas, the impact of enhanced lytic gene expression on the ab...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00770-12
更新日期:2012-08-01 00:00:00
abstract::The fate of polyomavirus genomes in stable transformants was examined in experiments in which rat or hamster cells were infected with mixed viral populations containing either two distinguishable wild types or a wild type plus a transformation-deficient mutant. The results demonstrate that in 90% of the cases in eithe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.3.1304-1313.1990
更新日期:1990-03-01 00:00:00
abstract::Q beta phage RNAs with inactivating insertion (8-base) or deletion (17-base) mutations within their replicase genes were prepared from modified Q beta cDNAs and transfected into Escherichia coli spheroplasts containing Q beta replicase provided in trans by a resident plasmid. Replicase-defective (Rep-) Q beta phage pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.4.2435-2442.1992
更新日期:1992-04-01 00:00:00
abstract::The copy choice model for the generation of defective interfering (DI) particles of vesicular stomatitis virus suggests that during replication the polymerase prematurely terminates, moves with the nascent daughter strand to another site on the same or a different template molecule, and resumes elongation of the nasce...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.2.515-521.1984
更新日期:1984-08-01 00:00:00
abstract::Adenine residues of 70S avian myeloblastosis virus (AMV) RNA are modified when reacted with chloroacetaldehyde. This modification introduces characteristic fluorescent epsilon-adenosine (epsilonA) probes which were used to monitor the reaction. Under suitable conditions, modified 70S(epsilonA) RNA was maintained intac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.5.1108-1114.1974
更新日期:1974-11-01 00:00:00
abstract::Polydnaviridae is a unique family of DNA viruses that are symbiotically associated with parasitoid wasps. Upon oviposition, wasps inject these viruses into their hosts, where they cause several physiological alterations, including suppression of the cellular immune response. Here we report that expression of the glc1....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.3.1861-1870.2005
更新日期:2005-02-01 00:00:00
abstract::Herpesviruses encode a set of core proteins essential for lytic replication of their genomes. Three of these proteins form a tripartite helix-primase complex that, in the case of Epstein-Barr virus (EBV), consists of the helicase BBLF4, the primase BSLF1, and the linker protein BBLF2/3. BBLF2/3 and its homologs in the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.1.245-256.2005
更新日期:2005-01-01 00:00:00
abstract::Rotavirus replication and virulence are strongly influenced by virus strain and host species. The rotavirus proteins VP3, VP4, VP7, NSP1, and NSP4 have all been implicated in strain and species restriction of replication; however, the mechanisms have not been fully determined. Simian (RRV) and bovine (UK) rotaviruses ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02408-10
更新日期:2011-03-01 00:00:00