Abstract:
:Q beta phage RNAs with inactivating insertion (8-base) or deletion (17-base) mutations within their replicase genes were prepared from modified Q beta cDNAs and transfected into Escherichia coli spheroplasts containing Q beta replicase provided in trans by a resident plasmid. Replicase-defective (Rep-) Q beta phage produced by these spheroplasts were detected as normal-sized plaques on lawns of cells containing plasmid-derived Q beta replicase, but were unable to form plaques on cells lacking this plasmid. When individual Rep- phage were isolated and grown to high titer in cells containing plasmid-derived Q beta replicase, revertant (Rep+) Q beta phage were obtained at a frequency of ca. 10(-8). To investigate the mechanism of this reversion, a point mutation was placed into the plasmid-derived Q beta replicase gene by site-directed mutagenesis. Q beta mutants amplified on cells containing the resultant plasmid also yielded Rep+ revertants. Genomic RNA was isolated from several of the latter phage revertants and sequenced. Results showed that the original mutation (insertion or deletion) was no longer present in the phage revertants but that the marker mutation placed into the plasmid was now present in the genomic RNAs, indicating that recombination was one mechanism involved in the reversion of the Q beta mutants. Further experiments demonstrated that the 3' noncoding region of the plasmid-derived replicase gene was necessary for the reversion-recombination of the deletion mutant, whereas this region was not required for reversion or recombination of the insertion mutant. Results are discussed in terms of a template-switching model of RNA recombination involving Q beta replicase, the mutant phage genome, and plasmid-derived replicase mRNA.
journal_name
J Viroljournal_title
Journal of virologyauthors
Palasingam K,Shaklee PNdoi
10.1128/JVI.66.4.2435-2442.1992subject
Has Abstractpub_date
1992-04-01 00:00:00pages
2435-42issue
4eissn
0022-538Xissn
1098-5514journal_volume
66pub_type
杂志文章abstract::Polydnaviridae is a unique family of DNA viruses that are symbiotically associated with parasitoid wasps. Upon oviposition, wasps inject these viruses into their hosts, where they cause several physiological alterations, including suppression of the cellular immune response. Here we report that expression of the glc1....
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pub_type: 杂志文章
doi:10.1128/JVI.79.3.1861-1870.2005
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pub_type: 杂志文章
doi:10.1128/JVI.02068-08
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.2.565-575.1983
更新日期:1983-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jvi.74.11.5066-5074.2000
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abstract::In 2002, severe acute respiratory syndrome (SARS)-coronavirus (CoV) appeared as a novel human virus with high similarity to bat coronaviruses. However, while SARS-CoV uses the human angiotensin-converting enzyme 2 (ACE2) receptor for cellular entry, no coronavirus isolated from bats appears to use ACE2. Here we show t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00311-12
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abstract::Papillomavirus disease poses a special challenge to people with compromised immune systems. Appropriate models to study infections in these individuals are lacking. We report here the development of a model that will help to address these deficiencies. The MmuPV1 genome was synthesized and used successfully to produce...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00777-13
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.8182-8186.1996
更新日期:1996-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.02275-08
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00019-20
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pub_type: 杂志文章
doi:10.1128/JVI.02307-14
更新日期:2015-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.3.1707-1711.1993
更新日期:1993-03-01 00:00:00
abstract::Forty isolates of herpes simplex virus were compared by means of cross-neutralization curves. The 11 oral isolates were serotype 1, and all 29 genital/anal isolates were serotype 2. The cytopathic effects of the two serotypes were consistently different. Passage of strains of type 1 and type 2 in mice and in rabbits y...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.5.1.51-59.1970
更新日期:1970-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02376-07
更新日期:2008-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01880-15
更新日期:2015-11-18 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.1.312-316.1985
更新日期:1985-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.3401-3406.1998
更新日期:1998-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2013-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2014-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00126-10
更新日期:2010-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.1.3.591-598.1967
更新日期:1967-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01961-09
更新日期:2010-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jvi.78.1.83-93.2004
更新日期:2004-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00505-12
更新日期:2012-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.1.463-466.1989
更新日期:1989-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02433-10
更新日期:2011-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01968-09
更新日期:2010-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.3.1775-1783.1996
更新日期:1996-03-01 00:00:00