Abstract:
:Forty isolates of herpes simplex virus were compared by means of cross-neutralization curves. The 11 oral isolates were serotype 1, and all 29 genital/anal isolates were serotype 2. The cytopathic effects of the two serotypes were consistently different. Passage of strains of type 1 and type 2 in mice and in rabbits yielded two variants, although the majority of the strains remained unchanged serologically and in their cytopathic effects. The two variants were derived from type 1 strains and differed from the parent strains in their cytopathic effects, each of them producing syncytia and enlarged plaques. They had, however, retained the serotypic properties and the deoxyribonucleic acid (DNA) densities of their parent strains. The Roizman syncytial/macroplaque strain of herpes simplex virus was also included in the study; the density of its DNA (1.727 g/ml) was typical of type 1 strains, and serologically it seemed to be basically a type 1 strain, although it was neutralized by type 2 antiserum slightly better than were other type 1 strains. Growth curves were performed of the two serotypes in rabbit kidney, human fibroblast, and mouse embryo tissue cultures. The type 2 strains attained lower titers of infectivity in these three cell systems; the levels of infectivity of type 2 virus in the culture fluid decreased much more rapidly after the maximum had been attained than did the levels of infectivity of the type 1 strains, due to the greater instability of the type 2 virus. Parallel titrations of different strains in tissue cultures and intracerebrally in mice indicated that the latter assay system was usually more sensitive for type 2 strains than it was for type 1 strains. The paralytic sequelae and inflammatory changes of lumbar ganglia and spinal cord in young rabbits inoculated extraneurally with strains of the two serotypes also indicate that the type 2 virus is more virulent in laboratory animals than is type 1 virus.
journal_name
J Viroljournal_title
Journal of virologyauthors
Plummer G,Waner JL,Phuangsab A,Goodheart CRdoi
10.1128/JVI.5.1.51-59.1970subject
Has Abstractpub_date
1970-01-01 00:00:00pages
51-9issue
1eissn
0022-538Xissn
1098-5514journal_volume
5pub_type
杂志文章abstract::The RNAs of replication-defective murine and primate type C transforming viruses were analyzed for the presence of nucleotide sequences homologous to the genomes of their respective helper type C viruses by using DNAs complementary (cDNA) to either the 5'-terminal (cDNA5') or total (cDNAtotal) nucleotide sequences of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.3.570-576.1978
更新日期:1978-06-01 00:00:00
abstract::We analyzed the CD4+ T-lymphocyte responses to dengue, West Nile, and yellow fever viruses 4 months after immunization of a volunteer with an experimental live-attenuated dengue virus type 1 vaccine (DEN-1 45AZ5). We examined bulk culture proliferation to noninfectious antigens, determined the precursor frequency of s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.10.5962-5967.1993
更新日期:1993-10-01 00:00:00
abstract::Some strains of the protozoan parasite genus Leishmania are persistently infected with single-segmented double-stranded RNA viruses, which are termed LRV. The function of these cytoplasmic viruses is unknown. In order to address the question of whether LRV affects the parasite's phenotype, pairs of isogenic LRV(+)-LRV...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.7.4122-4126.1995
更新日期:1995-07-01 00:00:00
abstract::Group I host range (hr) mutants of adenovirus type 5 are unable to transform rat embryo or rat embryo brain cells but induce an abnormal transformation of baby rat kidney cells. We established several transformed rat kidney cell lines and characterized them with respect to the transformed phenotype and the structure o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.2.674-685.1982
更新日期:1982-02-01 00:00:00
abstract::MVL2, an enveloped double-stranded DNA mycoplasmavirus, causes a nonlytic infection of Acholeplasma laidlawii leading to the establishment of a persistent infection. Persistently infected clones were found to be resistant to superinfection by homologous virus, but could be infected by heterologous virus. Cells in a pe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.1.254-261.1978
更新日期:1978-10-01 00:00:00
abstract::The level of RNase activity in human interferon preparations was examined. Although sequential purification of interferon resulted in nearly a 300-fold increase in specific activity, RNase-specific activity remained more or less constant. The implications of this finding for the analyses of the mode of action of inter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.3.1247-1248.1977
更新日期:1977-03-01 00:00:00
abstract::Infection by viruses depends on a balance between capsid stability and dynamics. This study investigated biologically and biotechnologically relevant aspects of the relationship in foot-and-mouth disease virus (FMDV) between capsid structure and thermostability and between thermostability and infectivity. In the FMDV ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02293-18
更新日期:2019-05-01 00:00:00
abstract::We report here the complete genomic sequence of the duck Tembusu virus (DTMUV) WJ-1 strain, isolated from Muscovy ducks. This is the first complete genome sequence of DTMUV reported in southern China. Compared with the other strains (TA, GH-2, YY5, and ZJ-407) that were previously found in eastern China, WJ-1 bears a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02361-12
更新日期:2012-12-01 00:00:00
abstract::Varicella-zoster virus (VZV) causes varicella and establishes latency in sensory nerve ganglia, but the characteristics of VZV latency are not well defined. Immunohistochemical detection of the VZV immediate-early 63 (IE63) protein in ganglion neurons has been described, but there are significant discrepancies in esti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02416-09
更新日期:2010-04-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) protease (PR) makes five obligatory cleavages in the viral Gag polyprotein precursor. The cleavage events release the virion structural proteins from the precursor and allow the virion to undergo maturation to become infectious. The protease cleavage between the matrix p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00317-09
更新日期:2009-09-01 00:00:00
abstract::All known DNA tumor viruses are known to target and inactivate two main cell cycle regulatory proteins, retinoblastoma protein (pRb) and p53. Inactivation of pRb promotes host cell cycle progression into S phase, and inactivation of p53 promotes cell immortalization. The DNA tumor virus Kaposi's sarcoma associated her...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.19.10336-10347.2004
更新日期:2004-10-01 00:00:00
abstract::The ATP-dependent DNase activity of Escherichia coli disappeared or was markedly reduced after infection with double-stranded DNA phages, T2, T3, T4, T5, T6, T7, lambda, phi80, and P1, but not with the single-stranded DNA phage f1, or the RNA phage Qbeta. This DNase activity was not reduced when chloramphenicol was ad...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.6.1611-1612.1974
更新日期:1974-12-01 00:00:00
abstract::Fowl adenovirus serotype 4 (FAdV-4) is a hepatotropic virus that causes severe hepatic damage characterized by basophilic intranuclear inclusion bodies, vacuolar degeneration, and multifocal necrosis in hepatocytes. Many aspects of FAdV-4 infection and pathogenesis, however, remain unknown. Here, we found that FAdV-4-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01938-20
更新日期:2020-12-23 00:00:00
abstract::Using gel mobility assays, we have detected two proteins that bind in the U3 region of the 1731 retrotransposon long terminal repeats (between positions -110 and -73) in nuclear extracts from Drosophila melanogaster cultured cells. The first one binds double-stranded DNA, whereas the other binds the mRNA-like strand i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.3.1682-1687.1992
更新日期:1992-03-01 00:00:00
abstract::The 5' untranslated region (UTR) of hepatitis C virus (HCV), which is composed of four domains (I, II, III, and IV) and a pseudoknot, is essential for translation and viral replication. Equine nonprimate hepacivirus (EHcV) harbors a 5' UTR consisting of a large 5'-terminal domain (I); three additional domains (I', II,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01997-17
更新日期:2018-03-14 00:00:00
abstract::Viruses are considered to use vesicular trafficking in infected cells, but the details of assembly/release pathways of hepatitis B virus (HBV) are still unknown. To identify key regulators of HBV production, we performed short interfering RNA (siRNA) screening for Rab proteins, which are considered to act as molecular...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00621-19
更新日期:2019-07-17 00:00:00
abstract::One characteristic of all positive-strand RNA viruses is the necessity to assemble viral RNA replication complexes on host intracellular membranes, a process whose molecular details are poorly understood. To study viral replication complex assembly we use the established model system of Flock House virus (FHV), which ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00040-09
更新日期:2009-05-01 00:00:00
abstract::A protamine-like protein named P6.9 is thought to play a role in the condensation of genomes of the baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) during an infection. Previous studies have shown that P6.9 is phosphorylated immediately upon synthesis and dephosphorylated upon the entry of th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00438-12
更新日期:2012-11-01 00:00:00
abstract::The stability of an Epstein-Barr virus (EBV)-simian virus 40 (SV40) hybrid shuttle vector, the p205-GTI plasmid, was analyzed in human cells during EBV- or SV40-type replication mode. When the p205-GTI plasmid was maintained as an episomal EBV vector in the human 293 cell line, no rearrangement was detected. To induce...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.9.3837-3843.1989
更新日期:1989-09-01 00:00:00
abstract::Using a DNA probe from an avian transforming virus, NK24, that contains the fos gene and that was newly isolated and characterized (M. Nishizawa, N. Goto, and S. Kawai, J. Virol. 61:3733-3740, 1987), we cloned and sequenced the chicken c-fos gene. Results showed that this gene, like the mouse or human c-fos gene, cont...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.12.4012-4018.1987
更新日期:1987-12-01 00:00:00
abstract::A comparison has been made of the membrane glycoproteins and glycopeptides from two enveloped viruses, Sindbis virus and vesicular stomatitis virus (VSV). Glycopeptides isolated from Sindbis virus and VSV grown in the same host appear to differ principally in the number of sialic acid residues per glycopeptide; when s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.6.2.176-182.1970
更新日期:1970-08-01 00:00:00
abstract::This report describes the results of experiments to determine whether chimeras between a retrovirus and portions of Ty3 are active in vivo. A chimera between Ty3 and a Neo(r)-marked Moloney murine leukemia virus (M-MuLV) was constructed. The C-terminal domain of M-MuLV integrase (IN) was replaced with the C-terminal d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.4297-4307.1998
更新日期:1998-05-01 00:00:00
abstract::Infectious bursal disease (IBD) is an acute, highly contagious, and immunosuppressive avian disease caused by IBD virus (IBDV). Although IBDV-induced host cell apoptosis has been established, the underlying molecular mechanism is still unclear. We report here that IBDV viral protein 5 (VP5) is a major apoptosis induce...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06104-11
更新日期:2012-02-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) infection of the human thymus results in depletion of CD4-bearing thymocytes. This depletion is initially manifested in the immature CD4+/CD8+ thymocyte subset. To determine cellular factors involved in HIV infection in the thymus, we examined the expression of the recently ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.9.6928-6934.1997
更新日期:1997-09-01 00:00:00
abstract::We have previously described the expression of the major nonstructural protein (NS-1) of Aleutian mink disease parvovirus (ADV) in insect cells by using a baculovirus vector (J. Christensen, T. Storgaard, B. Bloch, S. Alexandersen, and B. Aasted, J. Virol. 67:229-238, 1993). To study its biochemical properties, ADV NS...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.3.1802-1809.1995
更新日期:1995-03-01 00:00:00
abstract::Rhesus cytomegalovirus (RhCMV) infection of macaques exhibits strong similarities to human CMV (HCMV) persistence and pathogenesis. The immunogenicity of DNA vaccines encoding three RhCMV proteins (a truncated version of glycoprotein B lacking the transmembrane region and endodomain [gBDeltaTM], phosphoprotein 65-2 [p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01708-06
更新日期:2007-02-01 00:00:00
abstract::Ebola virus (EBOV) infections result in aggressive hemorrhagic fever in humans, with fatality rates reaching 90% and with no licensed specific therapeutics to treat ill patients. Advances over the past 5 years have firmly established monoclonal antibody (MAb)-based products as the most promising therapeutics for treat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01548-18
更新日期:2019-02-19 00:00:00
abstract::The configuration of latent varicella-zoster virus (VZV) DNA was analyzed by PCR. Template DNA for both internal and terminal VZV primers was present in a 1:1 ratio in ganglionic DNA, compared with a 15:1 ratio in DNA extracted from VZV virions, indicating that the VZV genomic termini are adjacent in latently infected...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.12.8151-8154.1995
更新日期:1995-12-01 00:00:00
abstract::The relationship between immunosenescence and the host response to virus infection is poorly understood at the molecular level. Two different patterns of pulmonary host responses to virus were observed when gene expression profiles from severe acute respiratory syndrome coronavirus (SARS-CoV)-infected young mice that ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00489-08
更新日期:2008-10-01 00:00:00
abstract:UNLABELLED:STING has emerged in recent years as a key player in orchestrating innate immune responses to cytosolic DNA and RNA derived from pathogens. However, the regulation of STING still remains poorly defined. In the present study, we investigated the mechanism of the regulation of STING expression in relation to t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00748-16
更新日期:2016-09-29 00:00:00