Cloned bacteriophage phi X174 DNA sequence interferes with synthesis of the complementary strand of infecting bacteriophage phi X174.

abstract：:Passage of foot-and-mouth disease virus (FMDV) in cell culture in the presence of the mutagenic base analog 5-fluorouracil or 5-azacytidine resulted in decreases of infectivity and occasional extinction of the virus. Low viral loads and low viral fitness enhanced the frequency of extinction events; this finding was sh...

journal_title：Journal of virology

authors： Sierra S,Dávila M,Lowenstein PR,Domingo E

更新日期：2000-09-01 00:00:00

abstract：:We have previously demonstrated that two mouse cell lines that are poorly infectible by reovirus become highly susceptible upon transfection with the gene encoding the epidermal growth factor receptor (EGFR) (J. E. Strong, D. Tang, and P. W. K. Lee, Virology 197:405-411, 1993). This enhancement of infection efficiency...

journal_title：Journal of virology

authors： Strong JE,Lee PW

更新日期：1996-01-01 00:00:00

abstract：:Retinoid (vitamin A) is an essential diet constituent that governs a broad range of biological processes. Its biologically active metabolite, all-trans retinoic acid (ATRA), exhibits a potent antiviral property by enhancing both innate and adaptive antiviral immunity against a variety of viral pathogens, such as, but ...

journal_title：Journal of virology

authors： Bang BR,Li M,Tsai KN,Aoyagi H,Lee SA,Machida K,Aizaki H,Jung JU,Ou JJ,Saito T

更新日期：2019-04-03 00:00:00

abstract：:Entry of the budded virus form of baculoviruses into insect and mammalian cells is generally thought to occur through a low-pH-dependent endocytosis pathway, possibly through clathrin-coated pits. This insight is primarily based on (immuno)electron microscopy studies but requires biochemical support to exclude the use...

journal_title：Journal of virology

authors： Long G,Pan X,Kormelink R,Vlak JM

更新日期：2006-09-01 00:00:00

abstract：:Tetherin (CD317/BST-2), an interferon-induced membrane protein, restricts the release of nascent retroviral particles from infected cell surfaces. While human immunodeficiency virus type 1 (HIV-1) encodes the accessory gene vpu to overcome the action of tetherin, the lineage of primate lentiviruses that gave rise to H...

journal_title：Journal of virology

authors： Le Tortorec A,Neil SJ

更新日期：2009-11-01 00:00:00

abstract：:We introduced various mutations into the activation and RNA binding domains of human immunodeficiency virus type 1 (HIV-1) Tat in order to develop a novel and potent transdominant Tat protein and to characterize its mechanism of action. The different mutant Tat proteins were characterized for their abilities to activa...

journal_title：Journal of virology

authors： Orsini MJ,Debouck CM

更新日期：1996-11-01 00:00:00

abstract：:Poliovirus proteinase 2A (2Apro) is autocatalytically released from the viral polyprotein by cleavage in cis of a Tyr-Gly dipeptide at its own amino terminus, resulting in separation of the P1 structural and P2-P3 nonstructural protein precursors. A second Ty-Gly dipeptide within 3D polymerase is cleaved by 2Apro in t...

journal_title：Journal of virology

authors： Hellen CU,Lee CK,Wimmer E

更新日期：1992-06-01 00:00:00

abstract：:Herpes simplex virus (HSV) latency in neurons remains poorly understood, and the heterogeneity of the sensory nervous system complicates mechanistic studies. In this study, we used primary culture of adult trigeminal ganglion (TG) mouse neurons in microfluidic devices and an in vivo model to examine the subtypes of se...

journal_title：Journal of virology

authors： Cabrera JR,Charron AJ,Leib DA

更新日期：2018-06-13 00:00:00

abstract：:DNA was extracted from [(3)H]thymidine-labeled Marek's disease virus (MDV) and purified by two cycles of CsCl gradient centrifugation in a fixed-angle rotor. The DNA was transcribed in vitro into (32)P-labeled complementary RNA (cRNA). MDV cRNA did not hybridize with DNA from chicken embryo fibroblast cultures or from...

journal_title：Journal of virology

authors： Nazerian K,Lindahl T,Klein G,Lee LF

更新日期：1973-10-01 00:00:00

abstract：:The human cytomegalovirus (HCMV) UL138 protein downregulates the cell surface expression of the multidrug resistance-associated protein 1 (MRP1) transporter. We examined the genetic requirements within UL138 for MRP1 downregulation. We determined that the acidic cluster dileucine motif is essential for UL138-mediated ...

journal_title：Journal of virology

authors： Gelbmann CB,Kalejta RF

更新日期：2019-05-15 00:00:00

abstract：:The replication of porcine endogenous retrovirus subgroup A (PERV-A) and PERV-B in certain human cell lines indicates that PERV may pose an infectious risk in clinical xenotransplantation. We have previously reported that human-tropic PERVs isolated from infected human cells following cocultivation with miniature swin...

journal_title：Journal of virology

authors： Wood JC,Quinn G,Suling KM,Oldmixon BA,Van Tine BA,Cina R,Arn S,Huang CA,Scobie L,Onions DE,Sachs DH,Schuurman HJ,Fishman JA,Patience C

更新日期：2004-03-01 00:00:00

abstract：:In this study, we have identified an internal ribosome entry site (IRES) from the highly infectious herpesvirus Marek's disease virus (MDV). The IRES was mapped to the intercistronic region (ICR) of a bicistronic mRNA that we cloned from the MDV-transformed CD4(+) T-cell line MSB-1. The transcript is a member of a fam...

journal_title：Journal of virology

authors： Tahiri-Alaoui A,Smith LP,Baigent S,Kgosana L,Petherbridge LJ,Lambeth LS,James W,Nair V

更新日期：2009-06-01 00:00:00

abstract：:The presence or absence of the receptor CD4 and the coreceptors CCR5 and CXCR4 restrict the cell tropism of human immunodeficiency virus type 1 (HIV-1). Despite the importance of thymic infection by HIV-1, conflicting reports regarding the expression of HIV-1 coreceptors on human thymocytes have not been resolved. We ...

journal_title：Journal of virology

authors： Taylor JR Jr,Kimbrell KC,Scoggins R,Delaney M,Wu L,Camerini D

更新日期：2001-09-01 00:00:00

abstract：:Type 1 human immunodeficiency viruses encoding mutated nef reading frames are 10- to 30-fold less infectious than are isogenic viruses in which the nef gene is intact. This defect in infectivity causes nef-negative viruses to grow at an attenuated rate in vitro. To investigate the mechanism of Nef-mediated enhancement...

journal_title：Journal of virology

authors： Pandori MW,Fitch NJ,Craig HM,Richman DD,Spina CA,Guatelli JC

更新日期：1996-07-01 00:00:00

abstract：:Dendritic cells (DCs) play a central role in instructing antiviral immune responses. DCs, however, can become targeted by different viruses themselves. We recently demonstrated that human DCs can be productively infected with echoviruses (EVs), but not coxsackie B viruses (CVBs), both of which are RNA viruses belongin...

journal_title：Journal of virology

authors： Kramer M,Schulte BM,Toonen LW,Barral PM,Fisher PB,Lanke KH,Galama JM,van Kuppeveld FJ,Adema GJ

更新日期：2008-03-01 00:00:00

abstract：:Latency-associated nuclear antigen 1 (LANA1) of Kaposi's sarcoma-associated herpesvirus (KSHV) is implicated in the persistence of the viral genome during latent infection. It has been suggested that LANA1 tethers the viral genome to the host chromosome and also participates actively in DNA replication from the termin...

journal_title：Journal of virology

authors： Lim C,Choi C,Choe J

更新日期：2004-07-01 00:00:00

abstract：:Because immunologic classification of human immunodeficiency virus type 1 (HIV) might be more relevant than genotypic classification for designing polyvalent vaccines, studies were undertaken to determine whether immunologically defined groups of HIV ("immunotypes") could be identified. For these experiments, the V3 r...

journal_title：Journal of virology

authors： Zolla-Pazner S,Gorny MK,Nyambi PN,VanCott TC,Nádas A

更新日期：1999-05-01 00:00:00

abstract：:The architecture of a retroviral genome presents some unusual features for transcriptional regulation because of duplication of the transcriptional control sequences in the 5' and 3' long terminal repeats (LTRs). We have studied the transcriptional activity of the 5' and 3' LTRs of human immunodeficiency virus type 1 ...

journal_title：Journal of virology

authors： Klaver B,Berkhout B

更新日期：1994-06-01 00:00:00

abstract：:The lymphoid cell line #1670 has been derived from the infiltrated spleen of a tumor-bearing marmoset monkey infected with Herpesvirus saimiri. The cells contain both types of H. saimiri DNA, unique light (L-) DNA (36% cytosine plus guanine) and repetitive heavy (H-) DNA (71% cytosine plus guanine), without producing ...

journal_title：Journal of virology

authors： Werner FJ,Bornkamm GW,Fleckenstein B

更新日期：1977-06-01 00:00:00

abstract：:VP16-H is an essential structural protein of herpes simplex virus type 1 (HSV-1) and is also a potent activator of virus immediate-early (IE) gene expression. Current models of functional determinants within VP16-H indicate that it consists of two domains, an N-terminal domain involved in recruiting VP16-H to a multic...

journal_title：Journal of virology

authors： Grapes M,O'Hare P

更新日期：2000-11-01 00:00:00

abstract：:Systemic application of first-generation adenovirus induces pathogenic effects in the liver. To begin unraveling the mechanisms underlying early liver toxicity after adenovirus infusion, particularly the role of macrophage activation and expression of viral genes in transduced target cells, first-generation adenovirus...

journal_title：Journal of virology

authors： Lieber A,He CY,Meuse L,Schowalter D,Kirillova I,Winther B,Kay MA

更新日期：1997-11-01 00:00:00

abstract：:The poliovirus-encoded, membrane-associated VPg-precursor polypeptide 3AB has been implicated in the initiation of viral RNA synthesis. We have expressed 3AB and 3A polypeptides in eukaryotic cells and examined their localization using indirect immunofluorescence and a direct in vitro membrane-binding assay. Results p...

journal_title：Journal of virology

authors： Datta U,Dasgupta A

更新日期：1994-07-01 00:00:00

abstract：:9-([2-Hydroxy-1-(hydroxymethyl)ethoxy]methyl)guanine (BW B759U) is more potent and has a more prolonged inhibitory effect against Epstein-Barr virus (EBV) in vitro than does acyclovir (ACV). To assess the mechanism of this difference, we first compared the extent of phosphorylation of the two drugs in superinfected Ra...

journal_title：Journal of virology

authors： Lin JC,Nelson DJ,Lambe CU,Choi EI

更新日期：1986-11-01 00:00:00

abstract：:Picornavirus RNAs are uncapped messengers and have unusually long 5' nontranslated regions (5'NTRs) which contain many noninitiating AUG triplets. The translational efficiency of different picornavirus RNAs varies between different cell-free extracts and even in the same extract, such as micrococcal nuclease-treated r...

journal_title：Journal of virology

authors： Jang SK,Kräusslich HG,Nicklin MJ,Duke GM,Palmenberg AC,Wimmer E

更新日期：1988-08-01 00:00:00

abstract：:Canine distemper virus attachment (hemagglutinin [H] equivalent) and fusion (F) antigens were purified by affinity chromatography with monoclonal antibodies. The purified antigens were used to immunize groups of three dogs. Radioimmune precipitation assays with sera from these animals showed that the F antigen prepara...

journal_title：Journal of virology

authors： Norrby E,Utter G,Orvell C,Appel MJ

更新日期：1986-05-01 00:00:00

abstract：:EBNA2 transcriptional activation and regulated EBNALP coactivation are critical for Epstein-Barr virus-infected primary B-lymphocyte growth transformation. EBNALP coactivation requires the EBNA2 acidic activation domain (E2AD); EBNALP can bind to E2AD. EBNALP has now been found to bind less well to EBNA2 amino acids 1...

journal_title：Journal of virology

authors： Peng CW,Zhao B,Kieff E

更新日期：2004-10-01 00:00:00

abstract：:The hepatitis B virus (HBV) X protein (HBx) is a multifunctional regulator of cellular signal transduction and transcription pathways and has a critical role in HBV replication. Much of the cytoplasmic signal transduction activity associated with HBx expression and its stimulation of viral replication is attributable ...

journal_title：Journal of virology

authors： Bouchard MJ,Wang L,Schneider RJ

更新日期：2006-05-01 00:00:00

abstract：:The nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid virus, Ad2(+)ND(1), differs from the defective Ad-SV40 hybrid populations previously described, in that this hybrid virus can replicate without the aid of nonhybrid adenovirus helper. Consequently, the deoxyribonucleic acid (DNA) from this virus, which ...

journal_title：Journal of virology

authors： Crumpacker CS,Henry PH,Kakefuda T,Rowe WP,Levin MJ,Lewis AM Jr

更新日期：1971-03-01 00:00:00

abstract：:Bovine bone marrow-derived macrophages infected with the cytopathic biotype of bovine viral diarrhea virus released an antiviral activity into the supernatant which was tentatively characterized as type I interferon because of its physicochemical properties. Such supernatants primed both infected and uninfected macrop...

journal_title：Journal of virology

authors： Adler B,Adler H,Pfister H,Jungi TW,Peterhans E

更新日期：1997-04-01 00:00:00

abstract：:A number of eucaryotic viruses have devised strategies to minimize the deleterious effects on protein synthesis caused by activation of the interferon-induced, double-stranded-RNA-activated protein kinase, P68. In a recent report, we described the down regulation of the P68 protein kinase in cells infected by human im...

journal_title：Journal of virology

authors： Roy S,Agy M,Hovanessian AG,Sonenberg N,Katze MG

更新日期：1991-02-01 00:00:00