Abstract:
:DNA was extracted from [(3)H]thymidine-labeled Marek's disease virus (MDV) and purified by two cycles of CsCl gradient centrifugation in a fixed-angle rotor. The DNA was transcribed in vitro into (32)P-labeled complementary RNA (cRNA). MDV cRNA did not hybridize with DNA from chicken embryo fibroblast cultures or from chicken spleen, but hybridized efficiently with DNA from MDV particles or MDV-infected cell cultures. Five Marek's disease tumors from different chickens and different organs (ovary, liver, testis) were all found to contain MDV DNA sequences. The relative amount of MDV DNA varied from tumor to tumor and was between 3 and 15 virus genome equivalents per cell. The content of virus DNA per cell in spleens from tumor-bearing chickens was much lower than in tumors from the same animals. MDV-infected cell cultures contained a large proportion (28-59%) of virus antigen-positive cells, as measured by immunofluorescence, but tumor cells were negative in this respect (<0.02% positive cells). These data indicate that MDV is present in a provirus form in tumor cells.
journal_name
J Viroljournal_title
Journal of virologyauthors
Nazerian K,Lindahl T,Klein G,Lee LFdoi
10.1128/JVI.12.4.841-846.1973subject
Has Abstractpub_date
1973-10-01 00:00:00pages
841-6issue
4eissn
0022-538Xissn
1098-5514journal_volume
12pub_type
杂志文章abstract::The members of Flaviviridae utilize several endocytic pathways to enter a variety of host cells. Our previous work showed that classical swine fever virus (CSFV) enters porcine kidney (PK-15) cells through a clathrin-dependent pathway that requires Rab5 and Rab7. The entry mechanism for CSFV into other cell lines rema...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00797-18
更新日期:2018-07-17 00:00:00
abstract:UNLABELLED:The role of NF-κB in influenza A virus (IAV) infection does not reveal a coherent picture, as pro- and also antiviral functions of this transcription factor have been described. To address this issue, we used clustered regularly interspaced short palindromic repeat with Cas9 (CRISPR-Cas9)-mediated genome eng...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00946-16
更新日期:2016-08-12 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02307-14
更新日期:2015-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.22.14149-14160.2005
更新日期:2005-11-01 00:00:00
abstract::Herpesvirus saimiri (HVS) is a T-cell-specific transforming and oncogenic virus. A protein encoded by HVS known as Tip-484 (for tyrosine kinase interacting protein from HVS strain 484) is required for this transformation. Tip-484 binds specifically to the nonreceptor protein tyrosine kinase p56lck. By transfecting Tip...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.378-382.1997
更新日期:1997-01-01 00:00:00
abstract:UNLABELLED:The distribution of vesicular stomatitis virus (VSV) nucleocapsids in the cytoplasm of infected cells was analyzed by scanning confocal fluorescence microscopy using a newly developed quantitative approach called the border-to-border distribution method. Nucleocapsids were located near the cell nucleus at ea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00488-16
更新日期:2016-06-10 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.8.5216-5224.1994
更新日期:1994-08-01 00:00:00
abstract::A recombinant capripoxvirus vaccine containing a cDNA of the peste-des-petits-ruminants virus (PPRV) fusion protein gene was constructed. A quick and efficient method was used to select a highly purified recombinant virus clone. A trial showed that a dose of this recombinant as low as 0.1 PFU protected goats against c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.2.1571-1577.2003
更新日期:2003-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.2.646-650.1981
更新日期:1981-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.5.2546-2551.1993
更新日期:1993-05-01 00:00:00
abstract::Human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) primarily infect activated CD4(+) T cells but can infect macrophages. Surprisingly, ex vivo tetramer-sorted SIV-specific CD8(+) T cells that eliminated and suppressed viral replication in SIV-infected CD4(+) T cells failed to do so in SIV-infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06324-11
更新日期:2012-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.12.7080-7088.1992
更新日期:1992-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01273-08
更新日期:2008-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.3.2084-2093.1999
更新日期:1999-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01347-09
更新日期:2010-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02360-15
更新日期:2016-01-20 00:00:00
abstract::The phenotypic defects of two mutants of vaccinia virus, the lesions of which map to gene G2R, were characterized in vivo, and the results suggest a role for the G2R protein in viral transcription elongation. Both a temperature-sensitive mutant, Cts56, and an isatin-beta-thiosemicarbazone-dependent deletion mutant, G2...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.1.47-54.1996
更新日期:1996-01-01 00:00:00
abstract::A DNA vaccine plasmid containing the Japanese encephalitis (JE) virus premembrane (prM) and envelope (E) genes (designated pcDNA3JEME) was evaluated for immunogenicity and protective efficacy in mice. Two immunizations of 4-week-old female ICR mice with pcDNA3JEME by intramuscular or intradermal injections at a dose o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.6.4925-4930.1998
更新日期:1998-06-01 00:00:00
abstract::The RNA genomes from the cardioviruses, hepatoviruses, and aphthoviruses encode two to five tandem pseudoknots within their 5' untranslated regions. These pseudoknots lie adjacent to a pyrimidine-rich sequence, which in cardio- and aphthoviruses takes the form of a homopolymeric poly(C) tract. Seven deletion mutations...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.8182-8186.1996
更新日期:1996-11-01 00:00:00
abstract::Broad, multispecific CD4(+) and CD8(+) T-cell responses to the hepatitis C virus (HCV), as well as virus-cross-neutralizing antibodies, are associated with recovery from acute infection and may also be associated in chronic HCV patients with a favorable response to antiviral treatment. In order to recapitulate all of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02743-07
更新日期:2008-08-01 00:00:00
abstract::Adenovirus type 7 (Ad7) early region 1 mRNA species transcribed in rat cell lines transformed by the HindIII-I . J fragment (the left 7.8% of the viral genome) and in human KB cells infected with Ad7 were mapped on the viral genome, using S1 nuclease gel and diazobenzyloxymethyl paper hybridization techniques. At the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.2.337-352.1980
更新日期:1980-11-01 00:00:00
abstract:UNLABELLED:Lethal mutagenesis is a broad-spectrum antiviral strategy that exploits the high mutation rate and low mutational tolerance of many RNA viruses. This approach uses mutagenic drugs to increase viral mutation rates and burden viral populations with mutations that reduce the number of infectious progeny. We inv...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03483-14
更新日期:2015-04-01 00:00:00
abstract::Human immunodeficiency virus infection in humans and simian immunodeficiency virus (SIV) infection in rhesus macaques (RM) leads to a generalized loss of immune responses involving perturbations in T-cell receptor (TCR) signaling. In contrast, naturally SIV-infected sooty mangabeys (SM) remain asymptomatic and retain ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.23.11298-11306.2001
更新日期:2001-12-01 00:00:00
abstract::T-antigen-induced DNA distortion was studied in a series of simian virus 40 (SV40) plasmid constructs whose relative replication efficiency ranges from 0.2 to 36. Bending was detected in the wild-type SV40 regulatory region consisting of three copies of the GC-rich 21-bp repeat but not in constructs with only one or t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.19.10651-10657.2003
更新日期:2003-10-01 00:00:00
abstract::Discovery of genetically distinct hantaviruses in multiple species of shrews (order Soricomorpha, family Soricidae) and moles (family Talpidae) contests the conventional view that rodents (order Rodentia, families Muridae and Cricetidae) are the principal reservoir hosts and suggests that the evolutionary history of h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02450-10
更新日期:2011-08-01 00:00:00
abstract::Herpes simplex virus 1 (HSV-1) is a common human pathogen that causes lifelong latent infection of sensory neurons. Non-nucleoside inhibitors that can limit HSV-1 recurrence are particularly useful in treating immunocompromised individuals or cases of emerging acyclovir-resistant strains of herpesvirus. We report that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01492-10
更新日期:2011-05-01 00:00:00
abstract::Most retroviral packaging cell lines were established by a helper virus plasmid cotransfected with a separate plasmid encoding a selection marker. Since this selection marker coexisted in trans with the helper virus sequence, helper virus gene expression could be inactivated by host DNA methylation despite selection f...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.11.5242-5249.2000
更新日期:2000-06-01 00:00:00
abstract::The 11th influenza A virus gene product is an 87-amino-acid protein provisionally named PB1-F2 (because it is encoded by an open reading frame overlapping the PB1 open reading frame). A significant fraction of PB1-F2 localizes to the inner mitochondrial membrane in influenza A virus-infected cells. PB1-F2 appears to e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.13.7214-7224.2003
更新日期:2003-07-01 00:00:00
abstract::The DNA sensing pathway triggers innate immune responses against DNA virus infection, and NF-κB signaling plays a critical role in establishing innate immunity. We report here that the herpes simplex virus 1 (HSV-1) ubiquitin-specific protease (UL36USP), which is a deubiquitinase (DUB), antagonizes NF-κB activation, d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02417-16
更新日期:2017-02-14 00:00:00
abstract::A total of 103 amber mutants of coliphage P1 were tested for lysis of nonpermissive cells. Of these, 83 caused cell lysis at the normal lysis time and have defects in particle morphogenesis. Five amber mutants, with mutations in the same gene (gene 2), caused premature lysis and may have a defect in a lysis regulator....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.2.519-530.1980
更新日期:1980-08-01 00:00:00