Abstract:
:In this study, we have identified an internal ribosome entry site (IRES) from the highly infectious herpesvirus Marek's disease virus (MDV). The IRES was mapped to the intercistronic region (ICR) of a bicistronic mRNA that we cloned from the MDV-transformed CD4(+) T-cell line MSB-1. The transcript is a member of a family of mRNAs expressed as immediate-early genes with two open reading frames (ORF). The first ORF encodes a 14-kDa polypeptide with two N-terminal splice variants, whereas the second ORF is contained entirely within a single exon and encodes a 12-kDa protein also known as RLORF9. We have shown that the ICR that separates the two ORFs functions as an IRES that controls the translation of RLORF9 when cap-dependent translation is inhibited. Deletion analysis revealed that there are two potential IRES elements within the ICR. Reverse genetic experiments with the oncogenic strain of MDV type 1 indicated that deletion of IRES-controlled RLORF9 does not significantly affect viral replication or MDV-induced mortality.
journal_name
J Viroljournal_title
Journal of virologyauthors
Tahiri-Alaoui A,Smith LP,Baigent S,Kgosana L,Petherbridge LJ,Lambeth LS,James W,Nair Vdoi
10.1128/JVI.02602-08subject
Has Abstractpub_date
2009-06-01 00:00:00pages
5846-53issue
11eissn
0022-538Xissn
1098-5514pii
JVI.02602-08journal_volume
83pub_type
杂志文章abstract::We present an antiviral-immunomodulatory therapeutic strategy involving the chemokine receptor antagonist Met-RANTES, which yields significant survival in the setting of an otherwise fatal respiratory virus infection. In previous work, we demonstrated that infection with the natural rodent pathogen pneumonia virus of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.15.7984-7989.2004
更新日期:2004-08-01 00:00:00
abstract::The virion proteins encoded by the avian retroviral pol gene (reverse transcriptase and endonuclease) are formed by the proteolytic processing of a gag-pol fusion protein precursor. Recent studies have predicted that the avian sarcoma-leukosis virus pol precursor protein undergoes a previously undetected processing ev...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.2.528-533.1988
更新日期:1988-02-01 00:00:00
abstract:UNLABELLED:The RNA genome of respiratory syncytial virus (RSV) is constitutively encapsidated by the viral nucleoprotein N, thus forming a helical nucleocapsid. Polymerization of N along the genomic and antigenomic RNAs is concomitant to replication and requires the preservation of an unassembled monomeric nucleoprotei...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03666-14
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abstract::The human cytomegalovirus UL47 open reading frame encodes a 110-kDa protein that is a component of the virion tegument. We have constructed a cytomegalovirus mutant, ADsubUL47, in which the central portion of the UL47 open reading frame has been replaced by two marker genes. The mutant replicated to titers 100-fold lo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.3.1043-1050.2002
更新日期:2002-02-01 00:00:00
abstract::The E5 oncoprotein of bovine papillomavirus type 1 is a 44-amino-acid, hydrophobic polypeptide which localizes predominantly in Golgi membranes and appears to transform cells through the activation of tyrosine kinase growth factor receptors. In fibroblasts, E5 interacts with both the 16-kilodalton vacuolar ATPase subu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.4.2420-2430.1996
更新日期:1996-04-01 00:00:00
abstract::Human erythroid progenitor cells are the main target cells of the human parvovirus B19 (B19), and B19 infection induces a transient erythroid aplastic crisis. Several authors have reported that the nonstructural protein 1 (NS-1) encoded by this virus has a cytotoxic effect, but the underlying mechanism of NS-1-induced...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8762-8770.1999
更新日期:1999-10-01 00:00:00
abstract::The glycoproteins of several enveloped viruses, grown in a variety of cell types, are labeled with 35SO4(-2), whereas the nonglycosylated proteins are not. This was shown for the HN and F glycoproteins of SV5 and Sendai virus, the E1 and E2 glycoproteins of Sindbis virus, and for the major glycoprotein, gp69, as well ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.4.859-866.1975
更新日期:1975-10-01 00:00:00
abstract::Inhibition of hepatitis B virus (HBV) replication and viral clearance from an infected host requires both the innate and adaptive immune responses. Expression of interferon (IFN)-inducible proteasome catalytic and regulatory subunits correlates with the IFN-alpha/beta- and IFN-gamma-mediated noncytopathic inhibition o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01779-06
更新日期:2007-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00423-08
更新日期:2008-07-01 00:00:00
abstract:UNLABELLED:Enterovirus 71 (EV71) recruits various cellular factors to assist in the replication and translation of its genome. Identification of the host factors involved in the EV71 life cycle not only will enable a better understanding of the infection mechanism but also has the potential to be of use in the developm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01677-15
更新日期:2015-10-01 00:00:00
abstract::ts5, a temperature-sensitive mutant of influenza B virus, belongs to one of seven recombination groups. When the mutant infected MDCK cells at the nonpermissive temperature (37.5 degrees C), infectious virus was produced at very low levels compared with the yield at the permissive temperature (32 degrees C) and hemagg...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.6.3264-3273.1993
更新日期:1993-06-01 00:00:00
abstract::Herpesvirus nucleocapsids traverse the nuclear envelope into the cytoplasm in a process called nuclear egress that includes disruption of the nuclear lamina. In several herpesviruses, a key player in nuclear egress is a complex of two proteins, whose homologs in human cytomegalovirus (HCMV) are UL50 and UL53. However,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02358-13
更新日期:2014-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.14.7903-7913.2003
更新日期:2003-07-01 00:00:00
abstract::Although hepatitis A virus (HAV) is typically transmitted by the fecal-oral route, little is known of its interactions with cells of the gastrointestinal tract. We studied the replication of HAV in polarized cultures of Caco-2 cells, a human cell line which retains many differentiated functions of small intestinal epi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.14.6476-6484.2000
更新日期:2000-07-01 00:00:00
abstract::The human T-cell leukemia virus type 1 (HTLV-1) envelope protein is required for virus spread. This study further characterizes the role of the envelope protein in HTLV-1 immortalization. Viruses with single amino acid substitutions within the SU protein at residue 75, 81, 95, 101, 105, or 195 or with a C-terminal cyt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.19.9553-9559.2001
更新日期:2001-10-01 00:00:00
abstract::In a study of the evolution and distribution of avian retroviruses, we found avian sarcoma and leukosis virus (ASLV) gag genes in 26 species of galliform birds from North America, Central America, eastern Europe, Asia, and Africa. Nineteen of the 26 host species from whom ASLVs were sequenced were not previously known...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.9.3984-3995.2000
更新日期:2000-05-01 00:00:00
abstract::Retroviral recombination can occur between two viral RNA molecules (intermolecular) or between two sequences within the same RNA molecule (intramolecular). The rate of retroviral intramolecular recombination is high. Previous studies showed that, after a single round of replication, 50 to 60% of retroviral recombinati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.19.9614-9623.2002
更新日期:2002-10-01 00:00:00
abstract::The monoclonal antibody PAb280 binds to small t antigen but not to large T antigen. Its binding site within the unique region of small t antigen was localized by studying its reaction with simian virus 40 mutants, other papovaviruses, and bacterial expression vectors coding for fragments of small t antigen. The antibo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.7.3128-3134.1989
更新日期:1989-07-01 00:00:00
abstract::We established a system for propagation of Sindbis virus (SIN)-based replicons in tissue culture in the form of a tricomponent genome virus. Three RNA fragments containing complementing genetic information required for virus replication are packaged into separate viral particles, and each cell produces at least 1,000 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.1.637-643.2005
更新日期:2005-01-01 00:00:00
abstract::Rat (3Y1) and hamster embryo brain cells were transformed by wild-type adenovirus type 12 or the DNA-minus temperature-sensitive mutant ts401. The ts401-transformed 3Y1 cells, but not the wild-type transformants, displayed a temperature-sensitive response with respect to the following characteristics of the transforme...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.1.250-260.1979
更新日期:1979-01-01 00:00:00
abstract::During secondary immune responses to influenza virus, virus-specific T memory cells are a major source of gamma interferon (IFN-gamma). We assessed the contribution of IFN-gamma to heterologous protection against the A/WSN/33 (H1N1) virus of wild-type and IFN-gamma-/- mice previously immunized with the A/HK/68 (H3N2) ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.8.6637-6645.1998
更新日期:1998-08-01 00:00:00
abstract::PM2 DNA was prepared with different superhelical densities (sigma) in order to examine the relationship betweenn supercoiling and the occurrence of a region(s) of unpaired bases in this DNA. A previous study showed that CH3HgOH reacts with native superhelical PM2 DNA more rapidly than the nicked form II. This evaluati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.1.195-204.1976
更新日期:1976-04-01 00:00:00
abstract::To study the expression of the Autographa californica nuclear polyhedrosis virus (AcNPV) genome, intracellular virus-specific proteins and mRNAs were pulsed-labeled, extracted, and analyzed at 6-h intervals during the replicative cycle. Most RNAs were detected between 12 and 24 h postinfection (p.i.), but many continu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.50.3.739-747.1984
更新日期:1984-06-01 00:00:00
abstract::The promoter P7535 of human papillomavirus type 8 and the promoter P7185 of bovine papillomavirus type 1 are negatively regulated by viral E2 proteins via the promoter proximal binding sites P2 and BS1, respectively. Mutations of these E2 binding sites can reduce basal promoter activity. This suggests binding of a tra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.10.8029-8034.1997
更新日期:1997-10-01 00:00:00
abstract:UNLABELLED:An understanding of the antigen-specific B-cell response to the influenza virus hemagglutinin (HA) is critical to the development of universal influenza vaccines, but it has not been possible to examine these cells directly because HA binds to sialic acid (SA) on most cell types. Here, we use structure-based...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03422-13
更新日期:2014-04-01 00:00:00
abstract::Retroviruses integrate into cellular DNA nonrandomly. Lentiviruses such as human immunodeficiency virus type 1 (HIV-1) favor the bodies of active genes and gene-enriched transcriptionally active regions of chromosomes. The interaction between lentiviral integrase and the cellular protein lens epithelium-derived growth...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01148-12
更新日期:2013-01-01 00:00:00
abstract::To study the function of the varicella-zoster virus (VZV) gB cytoplasmic domain during viral infection, we produced a VZV recombinant virus that expresses a truncated form of gB lacking the C-terminal 36 amino acids of its cytoplasmic domain (VZV gB-36). VZV gB-36 replicates in noncomplementing cells and grows at a ra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.2.591-599.2002
更新日期:2002-01-01 00:00:00
abstract::Walleye dermal sarcoma (WDS) and walleye epidermal hyperplasia (WEH) are skin diseases of walleye fish that appear and regress on a seasonal basis. We report here that the complex retroviruses etiologically associated with WDS (WDS virus [WDSV]) and WEH (WEH viruses 1 and 2 [WEHV1 and WEHV2, respectively]) encode D-ty...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.8765-8771.1998
更新日期:1998-11-01 00:00:00
abstract::Human cytomegalovirus (HCMV) encodes four putative G protein-coupled receptors, including pUL78, whose rodent orthologues are known to be important for replication and spread in their hosts. To investigate the mechanism by which pUL78 contributes to viral replication and pathogenesis, we generated a derivative of the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05900-11
更新日期:2012-11-01 00:00:00
abstract::To analyze the proposed growth-inhibitory function of wild-type p53, we compared simian virus 40 (SV40) DNA replication in primary rhesus monkey kidney (PRK) cells, which express wild-type p53, and in the established rhesus monkey kidney cell line LLC-MK2, which expresses a mutated p53 that does not complex with large...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.2.886-893.1993
更新日期:1993-02-01 00:00:00