当前位置： SCI文献检索 > JOURNAL OF VIROLOGY期刊下所有文献 > Human cytomegalovirus UL50 and UL53 recruit viral protein kinase UL97, not protein kinase C, for disruption of nuclear lamina and nuclear egress in infected cells.

Human cytomegalovirus UL50 and UL53 recruit viral protein kinase UL97, not protein kinase C, for disruption of nuclear lamina and nuclear egress in infected cells.

Abstract：

:Herpesvirus nucleocapsids traverse the nuclear envelope into the cytoplasm in a process called nuclear egress that includes disruption of the nuclear lamina. In several herpesviruses, a key player in nuclear egress is a complex of two proteins, whose homologs in human cytomegalovirus (HCMV) are UL50 and UL53. However, their roles in nuclear egress during HCMV infection have not been shown. Based largely on transfection studies, UL50 and UL53 have been proposed to facilitate disruption of the nuclear lamina by recruiting cellular protein kinase C (PKC), as occurs with certain other herpesviruses, and/or the viral protein kinase UL97 to phosphorylate lamins. To investigate these issues during HCMV infection, we generated viral mutants null for UL50 or UL53. Correlative light electron microscopic analysis of null mutant-infected cells showed the presence of intranuclear nucleocapsids and the absence of cytoplasmic nucleocapsids. Confocal immunofluorescence microscopy revealed that UL50 and UL53 are required for disruption of the nuclear lamina. A subpopulation of UL97 colocalized with the nuclear rim, and this was dependent on UL50 and, to a lesser extent, UL53. However, PKC was not recruited to the nuclear rim, and its localization was not affected by the absence of UL50 or UL53. Immunoprecipitation from cells infected with HCMV expressing tagged UL53 detected UL97 but not PKC. In summary, HCMV UL50 and UL53 are required for nuclear egress and disruption of nuclear lamina during HCMV infection, and they recruit UL97, not PKC, for these processes. Thus, despite the strong conservation of herpesvirus nuclear egress complexes, a key function can differ among them.

journal_name

J Virol

journal_title

Journal of virology

authors

Sharma M,Kamil JP,Coughlin M,Reim NI,Coen DM

doi

10.1128/JVI.02358-13

subject

Has Abstract

pub_date

2014-01-01 00:00:00

pages

249-62

issue

1

eissn

0022-538X

issn

1098-5514

pii

JVI.02358-13

journal_volume

88

pub_type

杂志文章

相关文献

JOURNAL OF VIROLOGY文献大全
  • CCR2 deficiency promotes exacerbated chronic erosive neutrophil-dominated chikungunya virus arthritis.

    abstract：UNLABELLED:Chikungunya virus (CHIKV) is a member of a globally distributed group of arthritogenic alphaviruses that cause weeks to months of debilitating polyarthritis/arthralgia, which is often poorly managed with current treatments. Arthritic disease is usually characterized by high levels of the chemokine CCL2 and a...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.03364-13

    authors： Poo YS,Nakaya H,Gardner J,Larcher T,Schroder WA,Le TT,Major LD,Suhrbier A

    更新日期：2014-06-01 00:00:00

  • Host microRNA regulation of human cytomegalovirus immediate early protein translation promotes viral latency.

    abstract：UNLABELLED:Reactivation of human cytomegalovirus (HCMV) is a significant cause of disease and death in immunocompromised patients, underscoring the need to understand how latency is controlled. Here we demonstrate that HCMV has evolved to utilize cellular microRNAs (miRNAs) in cells that promote latency to regulate exp...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.00481-14

    authors： O'Connor CM,Vanicek J,Murphy EA

    更新日期：2014-05-01 00:00:00

  • A novel polymorphism at codon 333 of human immunodeficiency virus type 1 reverse transcriptase can facilitate dual resistance to zidovudine and L-2',3'-dideoxy-3'-thiacytidine.

    abstract：:Recent clinical trials examining 3'-azido-3'-deoxythymidine (AZT, zidovudine, or Retrovir) combined with L-2', 3'-dideoxy-3'-thiacytidine (3TC or lamivudine) have shown that combination therapy with these nucleoside analogs affords significant virological and clinical benefits. The addition of 3TC to AZT delays AZT re...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.72.6.5093-5098.1998

    authors： Kemp SD,Shi C,Bloor S,Harrigan PR,Mellors JW,Larder BA

    更新日期：1998-06-01 00:00:00

  • Apoptosis-inducing and apoptosis-preventing functions of poliovirus.

    abstract：:Data showing that an apoptotic reaction (the exit into the cytoplasm and nucleolytic internucleosomal degradation of chromosomal DNA, compaction and fragmentation of chromatin, cellular shrinkage, and cytoplasmic blebbing) developed in a subline of HeLa-S3 cells upon nonpermissive poliovirus infection with either a gu...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.69.2.1181-1189.1995

    authors： Tolskaya EA,Romanova LI,Kolesnikova MS,Ivannikova TA,Smirnova EA,Raikhlin NT,Agol VI

    更新日期：1995-02-01 00:00:00

  • Transformation of T-lymphocyte subsets by Marek's disease herpesvirus.

    abstract：:Marek's disease herpesvirus (MDV)-transformed lymphoblastoid tumor cell lines were characterized for the presence of the surface markers. Monoclonal antibodies were used for CD3 (T-cell receptor [TCR] complex), TCR1, TCR2, and TCR3, CD4, CD8, and Ia antigen by indirect fluorescence staining followed by microscopic exa...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.65.3.1408-1413.1991

    authors： Schat KA,Chen CL,Calnek BW,Char D

    更新日期：1991-03-01 00:00:00

  • A 2.4-kilobase-pair fragment of the Friend murine leukemia virus genome contains the sequences responsible for friend murine leukemia virus-induced erythroleukemia.

    abstract：:Friend murine leukemia virus (F-MuLV) is a replication-competent, ecotropic, NB-tropic retrovirus which produces a rapidly fatal erythroleukemia in susceptible strains of mice. We previously molecularly cloned the entire F-MuLV genome. Transfection of this cloned DNA into NIH 3T3 mouse fibroblasts produces a virus wit...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.46.3.718-725.1983

    authors： Oliff A,Ruscetti S

    更新日期：1983-06-01 00:00:00

  • Role of the varicella-zoster virus gB cytoplasmic domain in gB transport and viral egress.

    abstract：:To study the function of the varicella-zoster virus (VZV) gB cytoplasmic domain during viral infection, we produced a VZV recombinant virus that expresses a truncated form of gB lacking the C-terminal 36 amino acids of its cytoplasmic domain (VZV gB-36). VZV gB-36 replicates in noncomplementing cells and grows at a ra...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/jvi.76.2.591-599.2002

    authors： Heineman TC,Hall SL

    更新日期：2002-01-01 00:00:00

  • Identification of deletion mutant respiratory syncytial virus strains lacking most of the G protein in immunocompromised children with pneumonia in South Africa.

    abstract：:Respiratory syncytial virus (RSV) G protein deletion mutants replicate effectively in vitro but have not been detected in nature. Subtyping of RSV strains in hospitalized children in South Africa identified G protein PCR amplicons significantly reduced in size in 2 out of 209 clinical specimens screened over 4 years. ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.00674-11

    authors： Venter M,van Niekerk S,Rakgantso A,Bent N

    更新日期：2011-08-01 00:00:00

  • Infection by retroviral vectors outside of their host range in the presence of replication-defective adenovirus.

    abstract：:Retrovirus infection is normally limited to cells within a specific host range which express a cognate receptor that is recognized by the product of the env gene. We describe retrovirus infection of cells outside of their normal host range when the infection is performed in the presence of a replication-defective aden...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.69.3.1887-1894.1995

    authors： Adams RM,Wang M,Steffen D,Ledley FD

    更新日期：1995-03-01 00:00:00

  • Equine herpesvirus 1 enters cells by two different pathways, and infection requires the activation of the cellular kinase ROCK1.

    abstract：:Equine herpesvirus type 1 (EHV-1), a member of the Alphaherpesviridae, displays a broad host range in vitro, allowing for detailed study of the mechanisms of productive infection, including attachment and entry, in various cell culture systems. Previously, we showed that EHV-1 infects Chinese hamster ovary (CHO-K1) ce...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.00504-07

    authors： Frampton AR Jr,Stolz DB,Uchida H,Goins WF,Cohen JB,Glorioso JC

    更新日期：2007-10-01 00:00:00

  • Simian immunodeficiency virus replicates to high levels in naturally infected African green monkeys without inducing immunologic or neurologic disease.

    abstract：:African green monkeys can maintain long-term persistent infection with simian immunodeficiency viruses (SIVagm) without developing AIDS and thus provide an important model for understanding mechanisms of natural host resistance to disease. This study assessed the levels and anatomic distribution of SIVagm in healthy, ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.75.5.2262-2275.2001

    authors： Broussard SR,Staprans SI,White R,Whitehead EM,Feinberg MB,Allan JS

    更新日期：2001-03-01 00:00:00

  • Dual tropism for macrophages and lymphocytes is a common feature of primary human immunodeficiency virus type 1 and 2 isolates.

    abstract：:We have investigated the ability of human immunodeficiency virus type 1 (HIV-1) and HIV-2 isolates to infect and replicate in primary human macrophages. Monocytes from blood donors were allowed to differentiate into macrophages by culture in the presence of autologous lymphocytes and human serum for 5 days before infe...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.68.10.6684-6689.1994

    authors： Valentin A,Albert J,Fenyö EM,Asjö B

    更新日期：1994-10-01 00:00:00

  • Generation of glucocorticoid-responsive Moloney murine leukemia virus by insertion of regulatory sequences from murine mammary tumor virus into the long terminal repeat.

    abstract：:The glucocorticoid-regulatory sequences from the murine mammary tumor virus long terminal repeat (MMTV LTR) were introduced into the LTR of Moloney murine leukemia virus (M-MuLV) by recombinant DNA techniques. The site of insertion was in the M-MuLV LTR U3 region at -150 base pairs with respect to the RNA cap site. In...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.54.1.133-144.1985

    authors： Overhauser J,Fan H

    更新日期：1985-04-01 00:00:00

  • Intracellular transport and secretion of hepatitis B surface antigen in mammalian cells.

    abstract：:The oligosaccharide processing and secretion of hepatitis B surface antigen (HBsAg) was studied in Chinese hamster ovary cells stably transfected with the gene coding HBsAg. HBsAg was secreted from cells with a relatively long half time (ca. 5 h). This appeared to be a characteristic of HBsAg itself, since HBsAg-produ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.51.2.346-353.1984

    authors： Patzer EJ,Nakamura GR,Yaffe A

    更新日期：1984-08-01 00:00:00

  • Persistent infections in L cells with temperature-sensitive mutants of reovirus.

    abstract：:Serial passage of reovirus temperature-sensitive (ts) mutant C(447) produced by passage 9 (P9) a heavily defective population of virus from which the double-stranded RNA genomic segments L(1), L(3), and M(1) were largely missing. Viral cores obtained from this P9 population were heterogeneous with respect to buoyant d...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.23.2.250-262.1977

    authors： Ahmed R,Graham AF

    更新日期：1977-08-01 00:00:00

  • Membrane binding of human immunodeficiency virus type 1 matrix protein in vivo supports a conformational myristyl switch mechanism.

    abstract：:The interaction of the human immunodeficiency virus (HIV) Gag protein with the plasma membrane of a cell is a critical event in the assembly of HIV particles. The matrix protein region (MA) of HIV type 1 (HIV-1) Pr55Gag has previously been demonstrated to confer membrane-binding properties on the precursor polyprotein...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.71.9.6582-6592.1997

    authors： Spearman P,Horton R,Ratner L,Kuli-Zade I

    更新日期：1997-09-01 00:00:00

  • Simian virus 40 replication pause sites map with the nucleosomes.

    abstract：:The locations of replication pause sites in the simian virus 40 minichromosome which were determined by sizing cloned fragments of nascent DNA (Zannis-Hadjopoulos et al., J. Mol. Biol. 165:599-607, 1983) were compared with the positions of simian virus 40 nucleosomes in the genome, as obtained by sequence-directed map...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.52.3.1011-1012.1984

    authors： Trifonov EN,Mengeritsky G

    更新日期：1984-12-01 00:00:00

  • Direct interaction between human cytomegalovirus glycoprotein B and cellular annexin II.

    abstract：:Cellular annexin II has been shown to specifically bind human cytomegalovirus (HCMV) and be a component of highly purified virions. In this report, we characterize the interaction of annexin II with HCMV. We found that the binding of annexin II to the HCMV envelope occurs partially through the calcium-dependent phosph...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.71.12.9803-9807.1997

    authors： Pietropaolo RL,Compton T

    更新日期：1997-12-01 00:00:00

  • Localization of cis-acting sequences in the latency-related promoter of bovine herpesvirus 1 which are regulated by neuronal cell type factors and immediate-early genes.

    abstract：:Bovine herpesvirus 1 (BHV-1) establishes a latent infection in sensory ganglionic neurons of cattle. During a latent infection, a single latency-related (LR) transcript is expressed. This observation suggested that DNA sequences in the LR promoter are positively regulated by neural cell type factors. The regulation of...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.66.10.6099-6106.1992

    authors： Bratanich AC,Jones CJ

    更新日期：1992-10-01 00:00:00

  • Targeting c-Mpl for revival of human immunodeficiency virus type 1-induced hematopoietic inhibition when CD34+ progenitor cells are re-engrafted into a fresh stromal microenvironment in vivo.

    abstract：:The inhibition of multilineage hematopoiesis which occurs in the severe combined immunodeficiency mouse with transplanted human fetal thymus and liver tissues (SCID-hu Thy/Liv) due to human immunodeficiency virus type 1 (HIV-1) infection is also accompanied by a severe loss of c-Mpl expression on these progenitor cell...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.78.20.11385-11392.2004

    authors： Koka PS,Kitchen CM,Reddy ST

    更新日期：2004-10-01 00:00:00

  • The amphotropic murine leukemia virus receptor gene encodes a 71-kilodalton protein that is induced by phosphate depletion.

    abstract：:The amphotropic murine leukemia virus (MuLV) can infect cells from a number of mammals, including humans, via its specific receptor. Basic knowledge of amphotropic MuLV receptor expression is likely to be useful in the development and improvement of gene therapy protocols based on amphotropic-pseudotyped vectors. To i...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.71.6.4564-4570.1997

    authors： Chien ML,Foster JL,Douglas JL,Garcia JV

    更新日期：1997-06-01 00:00:00

  • Insertion mutation of the int-1 and int-2 loci by mouse mammary tumor virus in premalignant and malignant neoplasms from the GR mouse strain.

    abstract：:Mouse mammary tumor virus (MMTV)-induced mammary adenocarcinomas can develop from several different premalignant precursors common in GR mice. Insertion mutagenesis of the mammary protooncogenes int-1 and int-2 was studied in this multistep system by analyzing samples from various stages of neoplastic development for ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.64.4.1794-1802.1990

    authors： Morris DW,Barry PA,Bradshaw HD Jr,Cardiff RD

    更新日期：1990-04-01 00:00:00

  • Kaposi's sarcoma-associated herpesvirus induces the ATM and H2AX DNA damage response early during de novo infection of primary endothelial cells, which play roles in latency establishment.

    abstract：UNLABELLED:The DNA damage response (DDR) that evolved to repair host cell DNA damage also recognizes viral DNA entering the nucleus during infections. Here, we investigated the modulation of DDR signaling during de novo infection of primary endothelial cells by Kaposi's sarcoma-associated herpesvirus (KSHV). Phosphoryl...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.03126-13

    authors： Singh VV,Dutta D,Ansari MA,Dutta S,Chandran B

    更新日期：2014-03-01 00:00:00

  • Neutrophils aid in protection of the vaginal mucosae of immune mice against challenge with herpes simplex virus type 2.

    abstract：:Large numbers of polymorphonuclear leukocytes (PMNs) infiltrated the murine vaginal mucosa within 24 h after intravaginal inoculation with an attenuated strain of herpes simplex virus type 2 (HSV-2). The role of these cells in resolution of a primary genital infection and in protection of HSV-immune animals against ch...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.73.8.6380-6386.1999

    authors： Milligan GN

    更新日期：1999-08-01 00:00:00

  • Structure of adeno-associated virus type 4.

    abstract：:Adeno-associated virus (AAV) is a member of the Parvoviridae, belonging to the Dependovirus genus. Currently, several distinct isolates of AAV are in development for use in human gene therapy applications due to their ability to transduce different target cells. The need to manipulate AAV capsids for specific tissue d...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.79.8.5047-5058.2005

    authors： Padron E,Bowman V,Kaludov N,Govindasamy L,Levy H,Nick P,McKenna R,Muzyczka N,Chiorini JA,Baker TS,Agbandje-McKenna M

    更新日期：2005-04-01 00:00:00

  • Helper-independent transformation by unintegrated Harvey sarcoma virus DNA.

    abstract：:We have studied the unintegrated infectious DNA of Harvey sarcoma virus (Ha-SV) and Moloney leukemia virus (Mo-MuLV). The source of infectious viral DNA was the Hirt supernatant fraction from cells acutely infected with Ha-SV and Mo-MuLV. To obtain a direct quantitative assay for infectious viral DNA, recipient mouse ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.26.2.291-298.1978

    authors： Lowy DR,Rands E,Scolnick EM

    更新日期：1978-05-01 00:00:00

  • Genome-wide histone acetylation profiling of Herpesvirus saimiri in human T cells upon induction with a histone deacetylase inhibitor.

    abstract：:Herpesviruses establish latency in suitable host cells after primary infection and persist in their host organisms for life. Most of the viral genes are silenced during latency, also enabling the virus to escape from an immune response. This study addresses the control of viral gene silencing by epigenetic mechanisms,...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.00164-11

    authors： Alberter B,Vogel B,Lengenfelder D,Full F,Ensser A

    更新日期：2011-06-01 00:00:00

  • Group A rotavirus infection and age-dependent diarrheal disease in rats: a new animal model to study the pathophysiology of rotavirus infection.

    abstract：:Group A rotaviruses are major pathogens causing acute gastroenteritis in children and animals. To determine if group A rotavirus replicates and induces disease in rats, antibody-negative Lewis neonatal or adult rats were inoculated orally with tissue culture-adapted human (Wa, WI61, and HAL1166), simian (rhesus rotavi...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/jvi.76.1.41-57.2002

    authors： Ciarlet M,Conner ME,Finegold MJ,Estes MK

    更新日期：2002-01-01 00:00:00

  • Segregation of EAV-HP ancient endogenous retroviruses within the chicken population.

    abstract：:Avian leukosis virus subgroup J (ALV-J), an exogenous avian retrovirus, is thought to have evolved by recombination with the highly identical env gene of the endogenous avian retrovirus EAV-HP. Embryonic expression of EAV-HP env has been suggested to be associated with the induction of immunological tolerance, a featu...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.75.23.11935-11938.2001

    authors： Sacco MA,Venugopal K

    更新日期：2001-12-01 00:00:00

  • Efficient production of human immunodeficiency virus proteins in transgenic mice.

    abstract：:Transgenic mice containing the complete human immunodeficiency virus (HIV) coding sequences fused to the mouse mammary tumor virus long terminal repeat were generated. They were found to produce high levels of authentic gag and env HIV proteins in several tissues known to support mouse mammary tumor virus-driven trans...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.66.6.3904-3908.1992

    authors： Jolicoeur P,Laperrière A,Beaulieu N

    更新日期：1992-06-01 00:00:00