Abstract:
:Mouse mammary tumor virus (MMTV)-induced mammary adenocarcinomas can develop from several different premalignant precursors common in GR mice. Insertion mutagenesis of the mammary protooncogenes int-1 and int-2 was studied in this multistep system by analyzing samples from various stages of neoplastic development for novel int-1 and int-2 restriction fragments generated by MMTV provirus integration. int-1 and int-2 insertion mutations were observed in both premalignant lesions and malignant tumors. Some of the tumors with insertion mutations were experimentally derived from insertion mutation-free premalignant precursors. Each class of neoplasm examined had a characteristic frequency of int-1 and int-2 insertion mutations; however, no correspondence was observed between neoplasm morphology and mutation of either gene. These results indicate that insertion mutation of the int-1 and int-2 loci by MMTV provirus can be involved in the earliest identifiable stages of neoplastic development as well as during progression of premalignant lesions to tumors. Insertion mutation of int-1 and int-2 is therefore not stage specific in this system.
journal_name
J Viroljournal_title
Journal of virologyauthors
Morris DW,Barry PA,Bradshaw HD Jr,Cardiff RDdoi
10.1128/JVI.64.4.1794-1802.1990subject
Has Abstractpub_date
1990-04-01 00:00:00pages
1794-802issue
4eissn
0022-538Xissn
1098-5514journal_volume
64pub_type
杂志文章abstract::The Sindbis virus minimal subgenomic mRNA promoter (spanning positions -19 to +5 relative to the subgenomic mRNA start site) is approximately three- to sixfold less active than the fully active -98 to +14 promoter region. We identified two elements flanking the -19 to +5 region which increase its transcription to leve...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.8.3509-3519.2001
更新日期:2001-04-01 00:00:00
abstract::The temporal sequence of coronavirus plus-strand and minus-strand RNA synthesis was determined in 17CL1 cells infected with the A59 strain of mouse hepatitis virus (MHV). MHV-induced fusion was prevented by keeping the pH of the medium below pH 6.8. This had no effect on the MHV replication cycle, but gave 5- to 10-fo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.1.328-334.1986
更新日期:1986-01-01 00:00:00
abstract::We have found that interleukin-4 (IL-4) abrogated constitutive beta interferon (IFN-beta) synthesis in mouse macrophages. Analysis of endogenous IFN mRNA by reverse transcription-PCR clearly documented the presence of IFN-beta but not of IFN-alpha transcripts in RNA from bone marrow-derived macrophages (BMM). Culture ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.10.6763-6766.1994
更新日期:1994-10-01 00:00:00
abstract::The bacteriophage T4 unf gene, known to be involved in the arrest of transcription from cytosine-containing DNA, is unessential except in Escherichia coli strains containing plasmid pR386. Comparative genetic and biochemical analyses of parameters of unf+ and unf- phage growth in host cells isogenic except for the pre...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.53.2.430-439.1985
更新日期:1985-02-01 00:00:00
abstract::In an attempt to generate broadly cross-reactive, neutralizing monoclonal antibodies (MAbs) to simian immunodeficiency virus (SIV), we compared two immunization protocols using different preparations of oligomeric SIV envelope (Env) glycoproteins. In the first protocol, mice were immunized with soluble gp140 (sgp140) ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.17.7922-7935.2000
更新日期:2000-09-01 00:00:00
abstract::RNA recombination is a major process in promoting rapid virus evolution in an infected host. A previous genome-wide screen with the yeast single-gene deletion library of 4,848 strains, representing approximately 80% of all genes of yeast, led to the identification of 11 host genes affecting RNA recombination in Tomato...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.3.1231-1241.2006
更新日期:2006-02-01 00:00:00
abstract::In-frame stop codons were introduced into the coding region of human immunodeficiency virus type 1 (HIV-1) transmembrane protein (gp41). Truncation of 147 amino acids from the carboxyl terminus of gp41 (TM709) significantly decreased the stability and cell surface expression of the viral Env proteins, while truncation...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.1.213-221.1993
更新日期:1993-01-01 00:00:00
abstract::5' S-isobutyl-adenosine (SIBA), a structural analogue of S-adenosylhomocysteine, reversibly blocks the multiplication of herpes simplex type 1 virus. In the presence of SIBA, viral protein synthesis is inhibited. After removing SIBA the synthesis of proteins starts rapidly again. The new polypeptides are mainly alpha ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.22.1.160-167.1977
更新日期:1977-04-01 00:00:00
abstract::To localize the epitopes recognized by monoclonal antibodies (MAbs) specific for the S1 subunit of the murine coronavirus JHMV spike protein, we have expressed S1 proteins with different deletions from the C terminus of S1. S1utt is composed of the entire 769-amino-acid (aa) S1 protein; S1NM, S1N, S1n(330), and S1n(22...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.5403-5410.1994
更新日期:1994-09-01 00:00:00
abstract::The locations of replication pause sites in the simian virus 40 minichromosome which were determined by sizing cloned fragments of nascent DNA (Zannis-Hadjopoulos et al., J. Mol. Biol. 165:599-607, 1983) were compared with the positions of simian virus 40 nucleosomes in the genome, as obtained by sequence-directed map...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.3.1011-1012.1984
更新日期:1984-12-01 00:00:00
abstract::A temperature-sensitive mutant of adenovirus 31 was defective in formation of the DNA replication complex, suggesting the existence of a virus-coded protein necessary for the complex-formation. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.2.538-540.1974
更新日期:1974-02-01 00:00:00
abstract::The fusogenic properties of the uncleaved spike (S) protein of murine coronavirus JHMV variant cl-2 were studied by expressing the S protein with a deleted putative cleavage site. The amino acid sequence of the putative cleavage site, Arg-Arg-Ala-Arg-Arg, was replaced by Arg-Thr-Ala-Leu-Glu by in vitro mutagenesis of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.3.1195-1202.1993
更新日期:1993-03-01 00:00:00
abstract::The protein (p59rel) encoded by the transforming gene of reticuloendotheliosis virus strain T (REV-T) has been identified in REV-T-transformed avian lymphoid cells by using antisera raised against synthetic peptides whose sequences were derived from three nonoverlapping regions of v-rel (N. R. Rice, T. D. Copeland, S....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.59.1.120-126.1986
更新日期:1986-07-01 00:00:00
abstract::Subgroup J avian leukosis virus (ALV-J) isolate GDKP1202 was isolated from a 50-day-old local yellow commercial broiler in the Guangdong province of China in 2012. Here we report the complete genomic sequence of the GDKP1202 isolate, which caused high mortality, serious growth suppression, thymic atrophy, and liver en...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02009-12
更新日期:2012-11-01 00:00:00
abstract::Chicken embryo fibroblasts and NIH 3T3 mouse cells were transformable by DNAs of chicken cells infected with avian myelocytomatosis virus strain MC29 or with avian erythroblastosis virus. Transfection of chicken cells appeared to require replication of MC29 or avian erythroblastosis virus in the presence of a nontrans...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.33.3.1199-1202.1980
更新日期:1980-03-01 00:00:00
abstract::Epstein-Barr virus (EBV) latent membrane protein 2A (LMP2A) is widely expressed in EBV-infected cells within the infected human host and EBV-associated malignancies, suggesting that LMP2A is important for EBV latency, persistence, and EBV-associated tumorigenesis. Previously, we demonstrated that LMP2A provides an ant...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00537-07
更新日期:2007-09-01 00:00:00
abstract:UNLABELLED:Aspects of intrinsic antiviral immunity are mediated by promyelocytic leukemia nuclear body (PML-NB) constituent proteins. During herpesvirus infection, these antiviral proteins are independently recruited to nuclear domains that contain infecting viral genomes to cooperatively promote viral genome silencing...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00426-16
更新日期:2016-06-10 00:00:00
abstract::The enhancer within the long terminal repeats (LTRs) of acquired somatic mouse mammary tumor viruses (MMTV) can activate juxtaposed genes and induce mammary tumors. In contrast, germ line proviral MMTV genomes are integrated in the host genome and considered to be genetically confined transcription units. Here we demo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8669-8676.1999
更新日期:1999-10-01 00:00:00
abstract::Proteolytic processing of the dengue virus polyprotein is mediated by host cell proteases and the virus-encoded NS2B-NS3 two-component protease. The NS3 protease represents an attractive target for the development of antiviral inhibitors. The three-dimensional structure of the NS3 protease domain has been determined, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.24.13708-13716.2004
更新日期:2004-12-01 00:00:00
abstract::Phage 2 adsorbed to Pseudomonas aeruginosa strain BI in 5 mM Tris buffer, providing that cations like Na(+), Mg(2+), or Ca(2+) were present. Adsorption was observed over a broad pH range, reaching a maximum level around pH 7.5, which coincided with the pH required for maximal activity of the phage 2-associated slime p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.1.22-27.1974
更新日期:1974-01-01 00:00:00
abstract::Infection of cells with poliovirus induces a massive intracellular membrane reorganization to form vesicle-like structures where viral RNA replication occurs. The mechanism of membrane remodeling remains unknown, although some observations have implicated components of the cellular secretory and/or autophagy pathways....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01820-06
更新日期:2007-01-01 00:00:00
abstract::The complex evolutionary process of human immunodeficiency virus type 1 (HIV-1) is marked by a high level of genetic variation. It has been shown that the HIV-1 genome is characterized by variable and more constant regions, unequal nucleotide frequencies, and preference for G-to-A substitutions. However, this knowledg...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.6.4761-4770.1997
更新日期:1997-06-01 00:00:00
abstract::The mutational alterations in polyoma virus mutants ts-a and ts-25E which cause their large T-antigens to be thermolabile have been identified. In ts-a, a G leads to A transition at nucleotide 2193 causes the replacement of Ala (GCT) by Thr (ACT). In ts-25E, a G leads to T transversion at nucleotide 2883 causes the re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.3.1094-1098.1981
更新日期:1981-03-01 00:00:00
abstract::Immune activation is a major characteristic of human immunodeficiency virus type 1 (HIV-1) infection and a strong prognostic factor for HIV-1 disease progression. The underlying mechanisms leading to immune activation in viremic HIV-1 infection, however, are not fully understood. Here we show that, following the initi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00421-07
更新日期:2007-08-01 00:00:00
abstract::We have developed a means to identify genes associated with particular aspects of virulence. By beginning with an avirulent deletion mutant of rabbitpox virus and systematically reintroducing overlapping segments of the deleted region, we have identified two regions of the viral genome associated with increased virule...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.3.1530-1542.1991
更新日期:1991-03-01 00:00:00
abstract::The infectious entry pathway of duck hepatitis B virus (DHBV) was investigated with primary duck hepatocytes. Virus uptake was measured by a selective PCR technique which allows for the detection of a successful infection without the need for viral replication or gene expression. To test whether DHBV uptake occurs by ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.5827-5831.1996
更新日期:1996-09-01 00:00:00
abstract::Rat lymphoid cells, TARS-1, immortalized by coculture with adult T-cell leukemia cells, were intraperitoneally injected into 65 newborn, inbred WKAH/Hkm rats. In most of the rats, tumor nodules were discernible 7 to 15 days after transplantation but were completely rejected within 5 to 6 weeks. Two rats with no tumor ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.11.6686-6694.1992
更新日期:1992-11-01 00:00:00
abstract::One characteristic of all positive-strand RNA viruses is the necessity to assemble viral RNA replication complexes on host intracellular membranes, a process whose molecular details are poorly understood. To study viral replication complex assembly we use the established model system of Flock House virus (FHV), which ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00040-09
更新日期:2009-05-01 00:00:00
abstract::Human respiratory syncytial virus (HRSV) is the leading cause of serious pediatric acute respiratory tract infections, and a better understanding is needed of the host response to HRSV and its attenuated vaccine derivatives. It has been shown previously that HRSV nonstructural proteins 1 and 2 (NS1 and NS2) inhibit th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.9.5353-5362.2005
更新日期:2005-05-01 00:00:00
abstract::The present study used the cottontail rabbit papillomavirus DNA-rabbit system to evaluate whether the regulatory genes E1 and E2 and the transforming gene E6 are required for papilloma formation. Frameshift mutations were generated in the individual genes in the context of a full-length cottontail rabbit papillomaviru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.6097-6102.1994
更新日期:1994-09-01 00:00:00