Abstract:
:To localize the epitopes recognized by monoclonal antibodies (MAbs) specific for the S1 subunit of the murine coronavirus JHMV spike protein, we have expressed S1 proteins with different deletions from the C terminus of S1. S1utt is composed of the entire 769-amino-acid (aa) S1 protein; S1NM, S1N, S1n(330), and S1n(220) are deletion mutants with 594, 453, 330, and 220 aa from the N terminus of the S1 protein. The expressed S1 deletion mutant proteins were examined for reactivities to a panel of MAbs. All MAbs classified in groups A and B, those reactive to most mouse hepatitis virus (MHV) strains and those specific for isolate JHMV, respectively, recognized S1N(330) and the larger S1 deletion mutants but failed to react with S1N(220). MAbs in group C, specific for the larger S protein of JHMV, reacted only with the S1utt protein without any deletion. These results indicated that the domain composed of the N-terminal 330 aa comprised the cluster of conformational epitopes recognized by MAbs in groups A and B. It was also shown that the epitopes of MAbs in group C were not restricted to the region missing in the smaller S protein. These results together with the fact that all MAbs in group B retained high neutralizing activity suggested the possibility that the N-terminal 330 aa are responsible for binding to the MHV-specific receptors. In investigate this possibility, we expressed the receptor protein and examined the binding of each S1 deletion mutant to the receptor. It was demonstrated that the S1N(330) protein as well as other S1 deletion mutants larger than S1N(330) bound to the receptor. These results indicated that a domain composed of 330 aa at the N terminus of the S1 protein is responsible for binding to the MHV-specific receptor.
journal_name
J Viroljournal_title
Journal of virologyauthors
Kubo H,Yamada YK,Taguchi Fdoi
10.1128/JVI.68.9.5403-5410.1994subject
Has Abstractpub_date
1994-09-01 00:00:00pages
5403-10issue
9eissn
0022-538Xissn
1098-5514journal_volume
68pub_type
杂志文章abstract::Changes in T-cell function are a hallmark of human immunodeficiency virus type 1 (HIV-1) infection, but the pathogenic mechanisms leading to these changes are unclear. We examined the gene expression profiles in ex vivo human CD4+ and CD8+ T cells from untreated HIV-1-infected individuals at different clinical stages ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01552-06
更新日期:2007-04-01 00:00:00
abstract::African swine fever virus induces in convalescent pigs antibodies that neutralized the virus before and after binding to susceptible cells, inhibiting both virus attachment and internalization. A further analysis of the neutralization mechanisms mediated by the different viral proteins showed that antibodies to protei...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.8.5689-5694.1996
更新日期:1996-08-01 00:00:00
abstract::The retrovirus type B leukemogenic virus (TBLV) causes T-cell lymphomas in mice. We have identified the Rorgamma locus as an integration site in 19% of TBLV-induced tumors. Overexpression of one or more Rorgamma isoforms in >77% of the tumors tested may complement apoptotic effects of c-myc overexpression. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.9.4943-4946.2004
更新日期:2004-05-01 00:00:00
abstract::Two major outer envelope glycoproteins of Epstein-Barr virus, gp350 and gp220, are known to be encoded by 3.2- and 2.5-kilobase RNAs which map to the same DNA fragment (M. Hummel, D. Thorley-Lawson, and E. Kieff, J. Virol. 49:413-417). These RNAs have the same 5' and 3' ends. The larger RNA is encoded by a 2,777-base ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.3.665-674.1985
更新日期:1985-06-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) tat protein functions at a much lower level in rodent cells than in human cells. This species-specific difference in trans activation appears to be due to the lack of a functional homolog of a human cofactor for tat in rodent cells. Using HIV-1 long terminal repeat-drive...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.9.4565-4567.1990
更新日期:1990-09-01 00:00:00
abstract::Activated Notch1 (AcN1) alleles cooperate with oncogenes from DNA tumor viruses in transformation of epithelial cells. AcN1 signaling has pleiotropic effects, and suggested oncogenic roles include driving proliferation through cyclin D1 or the generation of resistance to apoptosis on matrix withdrawal through a phosph...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.12.7106-7112.2003
更新日期:2003-06-01 00:00:00
abstract::Rhesus lymphocryptovirus (rLCV) and Epstein-Barr virus (EBV) are closely related gammaherpesviruses that infect and cause disease in rhesus monkeys and humans, respectively. Thus, rLCV is an important model system for EBV pathogenesis. Both rLCV and EBV express microRNAs (miRNAs), several conserved in sequence and gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00110-10
更新日期:2010-05-01 00:00:00
abstract::Half of the world's population is exposed to the risk of dengue virus infection. Although a vaccine for dengue virus is now available in a few countries, its reported overall efficacy of about 60% is not ideal. Protective immune correlates following natural dengue virus infection remain undefined, which makes it diffi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01096-16
更新日期:2016-11-28 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.18.9165-9175.2002
更新日期:2002-09-01 00:00:00
abstract::Passage of native classical swine fever virus (CSFV) in cultured swine kidney cells (SK6 cells) selects virus variants that attach to the surface of cells by interaction with membrane-associated heparan sulfate (HS). A Ser-to-Arg change in the C terminus of envelope glycoprotein E(rns) (amino acid 476 in the open read...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.20.9585-9595.2001
更新日期:2001-10-01 00:00:00
abstract::Epstein-Barr virus (EBV) undergoes latent and lytic replication cycles, and its reactivation from latency to lytic replication is initiated by expression of the two viral immediate-early transactivators, Zta and Rta. In vitro, reactivation of EBV can be induced by anti-immunoglobulin, tetradecanoyl phorbol acetate, an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01602-10
更新日期:2011-03-01 00:00:00
abstract::Epstein-Barr virus (EBV) uses tonsils as the portal of entry to establish persistent infection. EBV is found in various B-cell subsets in tonsils but exclusively in memory B cells in peripheral blood. The in vitro susceptibilities of B-cell subsets to EBV infection have been studied solely qualitatively. In this work,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02630-07
更新日期:2008-05-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) Tat protein is essential for viral replication and stimulates transcription of the integrated provirus by recruiting the kinase complex TAK/P-TEFb, composed of cyclin T1 (CycT1) and Cdk9, to the viral TAR RNA element. TAK/P-TEFb phosphorylates the RNA polymerase II compl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.21.10579-10587.2002
更新日期:2002-11-01 00:00:00
abstract::The combinatorial nature of genetic recombination can potentially provide organisms with immediate access to many more positions in sequence space than can be reached by mutation alone. Recombination features particularly prominently in the evolution of a diverse range of viruses. Despite rapid progress having been ma...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02152-08
更新日期:2009-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.7.4233-4241.1992
更新日期:1992-07-01 00:00:00
abstract::Reverse transcriptases (RTs) are found in a wide variety of mobile genetic elements including viruses, retrotransposons, and infectious organellar introns. An invariant triad of aspartates is thought to be required for the catalytic function of RTs. We generated RT mutants in the yeast retrotransposon Ty1, changing ea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.14.6337-6347.2001
更新日期:2001-07-01 00:00:00
abstract::The protease (PR) from human immunodeficiency virus (HIV) is essential for viral replication: this aspartyl protease, active only as a dimer, is responsible for cleavage of the viral polyprotein precursors (Gag and Gag-Pol), to release the functional mature proteins. In this work, we have studied the structure-functio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.15.8216-8226.2003
更新日期:2003-08-01 00:00:00
abstract::We analyzed xenotropic murine leukemia virus-related virus (XMRV) integration site sequences previously identified from human prostate tissues for single-nucleotide polymorphisms (SNPs) to discriminate between patient and potential cell line sources of the proviruses. The SNPs of two integration sites were identical t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05624-11
更新日期:2011-12-01 00:00:00
abstract::Hepatitis A virus (HAV), an atypical member of the Picornaviridae, grows poorly in cell culture. To define determinants of HAV growth, we introduced a blasticidin (Bsd) resistance gene into the virus genome and selected variants that grew at high concentrations of Bsd. The mutants grew fast and had increased rates of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01470-09
更新日期:2010-08-01 00:00:00
abstract::Vif counteracts the host restriction factor APOBEC3G (A3G) and other APOBEC3s by preventing the incorporation of A3G into progeny virions. We previously identified Vif mutants with a dominant-negative (D/N) phenotype that interfered with the function of wild-type Vif, inhibited the degradation of A3G, and reduced the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01708-19
更新日期:2020-03-17 00:00:00
abstract::When herpes simplex virus was inoculated into the flank of a BALB/c mouse by scarification, the local replication of virus was followed by the establishment of an acute ganglionic infection. The subsequent centrifugal spread of this virus along nerves to the skin of the whole dermatome led to the development of a band...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.53.3.944-948.1985
更新日期:1985-03-01 00:00:00
abstract::Genetic conflicts between retroviruses and their receptors result in the evolution of novel host entry restrictions and novel virus envelopes, and such variants can influence trans-species transmission. We screened rodents and other mammals for sequence variation in the Xpr1 receptor for the mouse xenotropic or polytr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01549-10
更新日期:2010-11-01 00:00:00
abstract::Lysyl-tRNA synthetase (LysRS) is packaged into human immunodeficiency virus type 1 (HIV-1) via its interaction with Gag, and this enzyme facilitates the selective packaging of tRNA(3)(Lys), the primer for initiating reverse transcription, into HIV-1. The Gag/LysRS interaction is detected at detergent-resistant membran...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.14.7553-7564.2004
更新日期:2004-07-01 00:00:00
abstract::Although picornavirus RNA genomes contain a 3'-terminal poly(A) tract that is critical for their replication, the impact of encephalomyocarditis virus (EMCV) infection on the host poly(A)-binding protein (PABP) remains unknown. Here, we establish that EMCV infection stimulates site-specific PABP proteolysis, resulting...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00896-12
更新日期:2012-10-01 00:00:00
abstract::In most prion diseases, infectivity accumulates in lymphoreticular organs early after infection. Defects in hematopoietic compartments, such as impaired B-cell maturation, or in stromal compartments, such as abrogation of follicular dendritic cells, can delay or prevent lymphoreticular prion colonization. However, the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.15.7097-7106.2001
更新日期:2001-08-01 00:00:00
abstract::The protein (VPg) linked to the 5' termini of poliovirus RNAs resolved into two species when subjected to non-equilibrium electrofocusing. The differently charged forms of VPg were not due to protein phosphorylation nor to variability of the number of phosphate residues associated with the nucleotide moiety remaining ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.3.863-871.1981
更新日期:1981-06-01 00:00:00
abstract::Coxsackievirus B3 (CVB3) is known to infect stem cells in the neonatal central nervous system. Here, we evaluated the effects of CVB3 infection on the major source and repository of stem cells, the bone marrow (BM). Viral genome was detectable in BM within 24 h of infection, and productive infection of BM cells was ev...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03004-12
更新日期:2013-03-01 00:00:00
abstract::During ϕX174 morphogenesis, 240 copies of the external scaffolding protein D organize 12 pentameric assembly intermediates into procapsids, a reaction reconstituted in vitro In previous studies, ϕX174 strains resistant to exogenously expressed dominant lethal D genes were experimentally evolved. Resistance was achieve...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01878-16
更新日期:2016-12-16 00:00:00
abstract:UNLABELLED:Lifelong antiretroviral therapy (ART) for HIV-1 does not diminish the established latent reservoir. A possible cure approach is to reactivate the quiescent genome from latency and utilize immune responses to eliminate cells harboring reactivated HIV-1. It is not known whether antibodies within HIV-1-infected...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02717-15
更新日期:2015-12-09 00:00:00
abstract::The tsB5 mutant of herpes simplex virus type 1 (HSV-1) strain HFEM was shown previously to be temperature sensitive for accumulation of the mature form of glycoprotein gB, for production or activity of a factor required in virus-induced cell fusion, and for production of virions with normal levels of infectivity. In a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.1.114-128.1980
更新日期:1980-07-01 00:00:00