Protective Capacity of the Human Anamnestic Antibody Response during Acute Dengue Virus Infection.

Abstract:

:Half of the world's population is exposed to the risk of dengue virus infection. Although a vaccine for dengue virus is now available in a few countries, its reported overall efficacy of about 60% is not ideal. Protective immune correlates following natural dengue virus infection remain undefined, which makes it difficult to predict the efficacy of new vaccines. In this study, we address the protective capacity of dengue virus-specific antibodies that are produced by plasmablasts a few days after natural secondary infection. Among a panel of 18 dengue virus-reactive human monoclonal antibodies, four groups of antibodies were identified based on their binding properties. While antibodies targeting the fusion loop of the glycoprotein of dengue virus dominated the antibody response, two smaller groups of antibodies bound to previously undescribed epitopes in domain II of the E protein. The latter, largely serotype-cross-reactive antibodies, demonstrated increased stability of binding at pH 5. These antibodies possessed weak to moderate neutralization capacity in vitro but were the most efficacious in promoting the survival of infected mice. Our data suggest that the cross-reactive anamnestic antibody response has a protective capacity despite moderate neutralization in vitro and a moderate decrease of viremia in vivo IMPORTANCE: Antibodies can protect from symptomatic dengue virus infection. However, it is not easy to assess which classes of antibodies provide protection because in vitro assays are not always predictive of in vivo protection. During a repeat infection, dengue virus-specific immune memory cells are reactivated and large amounts of antibodies are produced. By studying antibodies cloned from patients with heterologous secondary infection, we tested the protective value of the serotype-cross-reactive "recall" or "anamnestic" response. We found that results from in vitro neutralization assays did not always correlate with the ability of the antibodies to reduce viremia in a mouse model. In addition, a decrease of viremia in mice did not necessarily improve survival. The most protective antibodies were stable at pH 5, suggesting that antibody binding in the endosomes, after the antibody-virus complex is internalized, might be important to block virus spread in the organism.

journal_name

J Virol

journal_title

Journal of virology

authors

Xu M,Züst R,Toh YX,Pfaff JM,Kahle KM,Davidson E,Doranz BJ,Velumani S,Tukijan F,Wang CI,Fink K

doi

10.1128/JVI.01096-16

subject

Has Abstract

pub_date

2016-11-28 00:00:00

pages

11122-11131

issue

24

eissn

0022-538X

issn

1098-5514

pii

JVI.01096-16

journal_volume

90

pub_type

杂志文章
  • The measles virus hemagglutinin stalk: structures and functions of the central fusion activation and membrane-proximal segments.

    abstract:UNLABELLED:The measles virus (MeV) membrane fusion apparatus consists of a fusion protein trimer and an attachment protein tetramer. To trigger membrane fusion, the heads of the MeV attachment protein, hemagglutinin (H), bind cellular receptors while the 96-residue-long H stalk transmits the triggering signal. Structur...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02846-13

    authors: Navaratnarajah CK,Kumar S,Generous A,Apte-Sengupta S,Mateo M,Cattaneo R

    更新日期:2014-06-01 00:00:00

  • Evolution of the human immunodeficiency virus type 1 subtype-specific V3 domain is confined to a sequence space with a fixed distance to the subtype consensus.

    abstract::Human immunodeficiency virus type 1 (HIV-1) strains can be separated into genetic subtypes based on phylogenetic analysis of the envelope gene. Once it had been shown that population-wide intrasubtype genetic variation of HIV-1 strains increases in the course of the AIDS epidemic, it remained uncertain whether HIV-1 s...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.71.9.6332-6338.1997

    authors: Lukashov VV,Goudsmit J

    更新日期:1997-09-01 00:00:00

  • Human T-cell leukemia virus type 2 antisense viral protein 2 is dispensable for in vitro immortalization but functions to repress early virus replication in vivo.

    abstract::Human T-cell leukemia virus type 1 (HTLV-1) and HTLV-2 are closely related but pathogenically distinct human retroviruses. The antisense strand of the HTLV-1 genome encodes HTLV-1 basic leucine zipper (b-ZIP) protein (HBZ), a protein that inhibits Tax-mediated viral transcription, enhances T-cell proliferation, and pr...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00717-12

    authors: Yin H,Kannian P,Dissinger N,Haines R,Niewiesk S,Green PL

    更新日期:2012-08-01 00:00:00

  • Role of the influenza virus M1 protein in nuclear export of viral ribonucleoproteins.

    abstract::The protein kinase inhibitor H7 blocks influenza virus replication, inhibits production of the matrix protein (M1), and leads to a retention of the viral ribonucleoproteins (vRNPs) in the nucleus at late times of infection (K. Martin and A. Helenius, Cell 67:117-130, 1991). We show here that production of assembled vR...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.74.4.1781-1786.2000

    authors: Bui M,Wills EG,Helenius A,Whittaker GR

    更新日期:2000-02-01 00:00:00

  • RNA sequence determinants of a coupled termination-reinitiation strategy for downstream open reading frame translation in Helminthosporium victoriae virus 190S and other victoriviruses (Family Totiviridae).

    abstract::The genome-length, dicistronic mRNA of the double-stranded RNA fungal virus Helminthosporium victoriae virus 190S (genus Victorivirus, family Totiviridae) contains two long open reading frames (ORFs) that overlap in the tetranucleotide AUGA. Translation of the downstream ORF, which encodes the RNA-dependent RNA polyme...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00364-11

    authors: Li H,Havens WM,Nibert ML,Ghabrial SA

    更新日期:2011-07-01 00:00:00

  • The Epstein-Barr virus protein BRLF1 activates S phase entry through E2F1 induction.

    abstract::The Epstein-Barr Virus (EBV) immediate-early protein BRLF1 is one of two transactivators which mediate the switch from latent to lytic replication in EBV-infected cells. DNA viruses often modulate the function of critical cell cycle proteins to maximize the efficiency of virus replication. Here we have examined the ef...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.73.8.6540-6550.1999

    authors: Swenson JJ,Mauser AE,Kaufmann WK,Kenney SC

    更新日期:1999-08-01 00:00:00

  • Insertion mutation of the int-1 and int-2 loci by mouse mammary tumor virus in premalignant and malignant neoplasms from the GR mouse strain.

    abstract::Mouse mammary tumor virus (MMTV)-induced mammary adenocarcinomas can develop from several different premalignant precursors common in GR mice. Insertion mutagenesis of the mammary protooncogenes int-1 and int-2 was studied in this multistep system by analyzing samples from various stages of neoplastic development for ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.64.4.1794-1802.1990

    authors: Morris DW,Barry PA,Bradshaw HD Jr,Cardiff RD

    更新日期:1990-04-01 00:00:00

  • MyD88-dependent immune activation mediated by human immunodeficiency virus type 1-encoded Toll-like receptor ligands.

    abstract::Immune activation is a major characteristic of human immunodeficiency virus type 1 (HIV-1) infection and a strong prognostic factor for HIV-1 disease progression. The underlying mechanisms leading to immune activation in viremic HIV-1 infection, however, are not fully understood. Here we show that, following the initi...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00421-07

    authors: Meier A,Alter G,Frahm N,Sidhu H,Li B,Bagchi A,Teigen N,Streeck H,Stellbrink HJ,Hellman J,van Lunzen J,Altfeld M

    更新日期:2007-08-01 00:00:00

  • Phenotypic hiding: the carryover of mutations in RNA viruses as shown by detection of mar mutants in influenza virus.

    abstract::When influenza virus monoclonal antibody-resistant (mar) mutants are selected by incubation in vitro with excess antibody, 90 to 99% of the mutants are not detectable. This observation may be explained by encapsidation of mar mutant RNAs within phenotypically wild-type envelopes. This phenotypic hiding can be revealed...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.63.9.4107-4109.1989

    authors: Valcárcel J,Ortín J

    更新日期:1989-09-01 00:00:00

  • Keratinocytes are cell targets of West Nile virus in vivo.

    abstract::West Nile virus (WNV) replicates in the skin; however, cell targets in the skin have not been identified. In the current studies, WNV infected the epidermis and adnexal glands of mouse skin, and the epidermal cells were identified as keratinocytes by double labeling for WNV antigen and keratin 10. Inoculation of mice ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02692-10

    authors: Lim PY,Behr MJ,Chadwick CM,Shi PY,Bernard KA

    更新日期:2011-05-01 00:00:00

  • TRIM41-Mediated Ubiquitination of Nucleoprotein Limits Influenza A Virus Infection.

    abstract::Influenza A virus (IAV) is a highly transmissible respiratory pathogen and a major cause of morbidity and mortality around the world. Nucleoprotein (NP) is an abundant IAV protein essential for multiple steps of the viral life cycle. Our recent proteomic study of the IAV-host interaction network found that TRIM41 (tri...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00905-18

    authors: Patil G,Zhao M,Song K,Hao W,Bouchereau D,Wang L,Li S

    更新日期:2018-07-31 00:00:00

  • Basis for variable response of arboviruses to guanidine treatment.

    abstract::The effect of guanidine on the replication of the group A arboviruses, Sindbis virus, and Semliki Forest virus (SFV) was studied. Guanidine rapidly, but reversibly, inhibited SFV ribonucleic acid (RNA) synthesis. The synthesis of all species of viral RNA was inhibited, but that of ribonuclease-resistant forms was leas...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.6.5.628-636.1970

    authors: Friedman RM

    更新日期:1970-11-01 00:00:00

  • Cross-reactivity between hepatitis C virus and Influenza A virus determinant-specific cytotoxic T cells.

    abstract::The cellular immune response contributes to viral clearance as well as to liver injury in acute and chronic hepatitis C virus (HCV) infection. An immunodominant determinant frequently recognized by liver-infiltrating and circulating CD8(+) T cells of HCV-infected patients is the HCV(NS3-1073) peptide CVNGVCWTV. Using ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.75.23.11392-11400.2001

    authors: Wedemeyer H,Mizukoshi E,Davis AR,Bennink JR,Rehermann B

    更新日期:2001-12-01 00:00:00

  • Contrasting effects from a single major histocompatibility complex class II molecule (H-2E) in recovery from Friend virus leukemia.

    abstract::Resistance to erythroleukemia induced by infection with the Friend virus complex (FV) has been mapped to several genes residing both within and outside the murine major histocompatibility complex (MHC). MHC genes located in the A, D, and Qa/Tla regions of the murine H-2 complex have been shown to affect disease resist...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.68.8.4921-4926.1994

    authors: Perry LL,Miyazawa M,Hasenkrug K,Wehrly K,David CS,Chesebro B

    更新日期:1994-08-01 00:00:00

  • Morphological comparison of Machupo with lymphocytic choriomeningitis virus: basis for a new taxonomic group.

    abstract::Striking morphologic similarities between Machupo, Tacaribe, and lymphocytic choriomeningitis viruses were found by thin-section electron microscopy. It is proposed that these viruses be brought together into a single taxonomic group. ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.4.4.535-541.1969

    authors: Murphy FA,Webb PA,Johnson KM,Whitfield SG

    更新日期:1969-10-01 00:00:00

  • Avian retroviral RNA encapsidation: reexamination of functional 5' RNA sequences and the role of nucleocapsid Cys-His motifs.

    abstract::RNA packaging signals (psi) from the 5' ends of murine and avian retroviral genomes have previously been shown to direct encapsidation of heterologous mRNA into the retroviral virion. The avian 5' packaging region has now been further characterized, and we have defined a 270-nucleotide sequence, A psi, which is suffic...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.67.1.178-188.1993

    authors: Aronoff R,Hajjar AM,Linial ML

    更新日期:1993-01-01 00:00:00

  • Roles of Cholesterol in Early and Late Steps of the Nipah Virus Membrane Fusion Cascade.

    abstract::Cholesterol has been implicated in various viral life cycle steps for different enveloped viruses, including viral entry into host cells, cell-cell fusion, and viral budding from infected cells. Enveloped viruses acquire their membranes from their host cells. Though cholesterol has been associated with binding and ent...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02323-20

    authors: Contreras EM,Johnston GP,Buchholz DW,Ortega V,Monreal IA,Zamora JLR,Cheung T,Aguilar HC

    更新日期:2021-01-06 00:00:00

  • Retroviral gag gene amber codon suppression is caused by an intrinsic cis-acting component of the viral mRNA.

    abstract::In some type C retroviruses, translation of the pol gene appears to require translational suppression of the proximal gag amber codon. To identify the region of the viral nucleic acid responsible for synthesis of the pol gene products, a 300-base-pair DNA fragment containing the stop codon from a type C murine virus (...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.62.10.3574-3580.1988

    authors: Panganiban AT

    更新日期:1988-10-01 00:00:00

  • Herpes simplex virus 1 recombinants with noninverting genomes frozen in different isomeric arrangements are capable of independent replication.

    abstract::Herpes simplex virus 1 genome consists of two covalently linked components, L and S, that invert relative to each other to yield four equimolar isomeric populations designated P (prototype), Is (inversion of S component), Il (inversion of L component), and Ils (inversion of L and S components) differing in the orienta...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.59.2.494-499.1986

    authors: Jenkins FJ,Roizman B

    更新日期:1986-08-01 00:00:00

  • The Ig-like v-type domain of paired Ig-like type 2 receptor alpha is critical for herpes simplex virus type 1-mediated membrane fusion.

    abstract::Paired immunoglobulin (Ig)-like type 2 receptor alpha (PILRalpha) and PILRbeta are paired receptors that are highly homologous to each other. When engaged by ligand, PILRalpha is inhibitory whereas PILRbeta is activating. PILRalpha is a newly identified herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) receptor ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01039-10

    authors: Fan Q,Longnecker R

    更新日期:2010-09-01 00:00:00

  • Nucleotide sequence of VP4 and VP7 genes of human rotaviruses with subgroup I specificity and long RNA pattern: implication for new G serotype specificity.

    abstract::We sequenced the genes coding for the two neutralization proteins, VP4 and VP7, of human rotavirus strains L26 and L27 with subgroup I specificity but the long RNA pattern. The deduced VP7 amino acid sequence of strains L26 and L27 showed a low homology (73.6 to 81.9%) to those of rotavirus strains of the established ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.64.11.5640-5644.1990

    authors: Taniguchi K,Urasawa T,Kobayashi N,Gorziglia M,Urasawa S

    更新日期:1990-11-01 00:00:00

  • Tyr82 Amino Acid Mutation in PB1 Polymerase Induces an Influenza Virus Mutator Phenotype.

    abstract::In various positive-sense single-stranded RNA viruses, a low-fidelity viral RNA-dependent RNA polymerase (RdRp) confers attenuated phenotypes by increasing the mutation frequency. We report a negative-sense single-stranded RNA virus RdRp mutant strain with a mutator phenotype. Based on structural data of RdRp, rationa...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00834-19

    authors: Naito T,Shirai K,Mori K,Muratsu H,Ushirogawa H,Ohniwa RL,Hanada K,Saito M

    更新日期:2019-10-29 00:00:00

  • Human immunodeficiency virus mutagenesis during antiviral therapy: impact of drug-resistant reverse transcriptase and nucleoside and nonnucleoside reverse transcriptase inhibitors on human immunodeficiency virus type 1 mutation frequencies.

    abstract::The development of antiviral drug resistance is an important problem in the treatment of human immunodeficiency virus type 1 (HIV-1) infection. Potent antiretroviral therapy is currently used for treatment, and typically consists of at least two reverse transcriptase (RT) inhibitors. We have previously reported that b...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.79.18.12045-12057.2005

    authors: Chen R,Yokoyama M,Sato H,Reilly C,Mansky LM

    更新日期:2005-09-01 00:00:00

  • Disulfide bond configuration of human cytomegalovirus glycoprotein B.

    abstract::Glycoprotein B (gB) is the most highly conserved of the envelope glycoproteins of human herpesviruses. The gB protein of human cytomegalovirus (CMV) serves multiple roles in the life cycle of the virus. To investigate structural properties of gB that give rise to its function, we sought to determine the disulfide bond...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.76.12.6073-6082.2002

    authors: Lopper M,Compton T

    更新日期:2002-06-01 00:00:00

  • Systemic immunization with an ALVAC-HIV-1/protein boost vaccine strategy protects rhesus macaques from CD4+ T-cell loss and reduces both systemic and mucosal simian-human immunodeficiency virus SHIVKU2 RNA levels.

    abstract::Transmission of human immunodeficiency virus type 1 (HIV-1) occurs primarily via the mucosal route, suggesting that HIV-1 vaccines may need to elicit mucosal immune responses. Here, we investigated the immunogenicity and relative efficacy of systemic immunization with two human ALVAC-HIV-1 recombinant vaccines express...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.80.8.3732-3742.2006

    authors: Pal R,Venzon D,Santra S,Kalyanaraman VS,Montefiori DC,Hocker L,Hudacik L,Rose N,Nacsa J,Edghill-Smith Y,Moniuszko M,Hel Z,Belyakov IM,Berzofsky JA,Parks RW,Markham PD,Letvin NL,Tartaglia J,Franchini G

    更新日期:2006-04-01 00:00:00

  • VP3, a structural protein of infectious pancreatic necrosis virus, interacts with RNA-dependent RNA polymerase VP1 and with double-stranded RNA.

    abstract::Infectious pancreatic necrosis virus (IPNV) is a bisegmented, double-stranded RNA (dsRNA) virus of the Birnaviridae family that causes widespread disease in salmonids. Its two genomic segments are encapsulated together with the viral RNA-dependent RNA polymerase, VP1, and the assumed internal protein, VP3, in a single...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02831-06

    authors: Pedersen T,Skjesol A,Jørgensen JB

    更新日期:2007-06-01 00:00:00

  • Extensive genetic variability of simian immunodeficiency virus from African green monkeys.

    abstract::Serological surveys have revealed that 30 to 50% of wild-caught African green monkeys have antibodies reactive to simian immunodeficiency virus (SIV), a retrovirus related to human immunodeficiency virus (HIV). Although the nucleotide sequence of one SIVagm isolate, Tyo1, was recently reported, the extent of genetic v...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.63.4.1800-1802.1989

    authors: Li Y,Naidu YM,Daniel MD,Desrosiers RC

    更新日期:1989-04-01 00:00:00

  • Changes in RNA expression pattern during the malignant progression of cottontail rabbit papillomavirus-induced tumors in rabbits.

    abstract::Cottontail rabbit papillomavirus induces strictly epithelial tumors in both cottontail and domestic rabbits. A high proportion of the initial benign papillomas progress within 8 to 14 months to invasive carcinomas. With the help of mRNA-specific riboprobes for E6, E7, E1, E2, L1 and L2, we investigated by in situ hybr...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.68.6.3620-3630.1994

    authors: Zeltner R,Borenstein LA,Wettstein FO,Iftner T

    更新日期:1994-06-01 00:00:00

  • The large delta antigen of hepatitis delta virus potently inhibits genomic but not antigenomic RNA synthesis: a mechanism enabling initiation of viral replication.

    abstract::Hepatitis delta virus (HDV) contains two types of hepatitis delta antigens (HDAg) in the virion. The small form (S-HDAg) is required for HDV RNA replication, whereas the large form (L-HDAg) potently inhibits it by a dominant-negative inhibitory mechanism. The sequential appearance of these two forms in the infected ce...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.74.16.7375-7380.2000

    authors: Modahl LE,Lai MM

    更新日期:2000-08-01 00:00:00

  • Properties of hamster embryo fibroblasts transformed in vitro after exposure to ultraviolet-irradiated herpes simplex virus type 2.

    abstract::An in vitro method which led to the transformation of hamster embryo fibroblasts after exposure to herpes simplex virus type 2 (HSV-2) inactivated with ultraviolet irradiation is described. The transformed cells (333-8-9) produced tumors when inoculated into newborn Syrian hamsters but not when injected into weanling ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.8.4.469-477.1971

    authors: Duff R,Rapp F

    更新日期:1971-10-01 00:00:00