Abstract:
:The combinatorial nature of genetic recombination can potentially provide organisms with immediate access to many more positions in sequence space than can be reached by mutation alone. Recombination features particularly prominently in the evolution of a diverse range of viruses. Despite rapid progress having been made in the characterization of discrete recombination events for many species, little is currently known about either gross patterns of recombination across related virus families or the underlying processes that determine genome-wide recombination breakpoint distributions observable in nature. It has been hypothesized that the networks of coevolved molecular interactions that define the epistatic architectures of virus genomes might be damaged by recombination and therefore that selection strongly influences observable recombination patterns. For recombinants to thrive in nature, it is probably important that the portions of their genomes that they have inherited from different parents work well together. Here we describe a comparative analysis of recombination breakpoint distributions within the genomes of diverse single-stranded DNA (ssDNA) virus families. We show that whereas nonrandom breakpoint distributions in ssDNA virus genomes are partially attributable to mechanistic aspects of the recombination process, there is also a significant tendency for recombination breakpoints to fall either outside or on the peripheries of genes. In particular, we found significantly fewer recombination breakpoints within structural protein genes than within other gene types. Collectively, these results imply that natural selection acting against viruses expressing recombinant proteins is a major determinant of nonrandom recombination breakpoint distributions observable in most ssDNA virus families.
journal_name
J Viroljournal_title
Journal of virologyauthors
Lefeuvre P,Lett JM,Varsani A,Martin DPdoi
10.1128/JVI.02152-08subject
Has Abstractpub_date
2009-03-01 00:00:00pages
2697-707issue
6eissn
0022-538Xissn
1098-5514pii
JVI.02152-08journal_volume
83pub_type
杂志文章abstract::Several aspects of the terminal stages of T4 head maturation were investigated using ts and am mutants blocked at single steps of the assembly pathway. We had previously found that cells infected with mutants of gene 13, e.g., tsN38 and amE609, accumulated both stable (10 to 20%)- and fragile (80%)-filled head precurs...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.2.550-567.1976
更新日期:1976-02-01 00:00:00
abstract:UNLABELLED:The potency and breadth of the recently isolated neutralizing human monoclonal antibodies to HIV-1 have stimulated interest in their use to prevent or to treat HIV-1 infection. Due to the antigenically diverse nature of the HIV-1 envelope (Env), no single antibody is highly active against all viral strains. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02097-15
更新日期:2015-12-01 00:00:00
abstract::Sequence analysis of the Lymantria dispar multicapsid nucleopolyhedrovirus (LdMNPV) genome identified an open reading frame (ORF) encoding a 548-amino-acid (62-kDa) protein that showed 35% amino acid sequence identity with vaccinia virus ATP-dependent DNA ligase. Ligase homologs have not been reported from other bacul...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.9142-9149.1998
更新日期:1998-11-01 00:00:00
abstract::The adenovirus fiber protein is used for attachment of the virus to a specific receptor on the cell surface. Structurally, the protein consists of a long, thin shaft that protrudes from the vertex of the virus capsid and terminates in a globular domain termed the knob. To verify that the knob is the domain which inter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.8.5239-5246.1994
更新日期:1994-08-01 00:00:00
abstract::The susceptibility of monocyte-derived cultured dendritic cells (DCs) to human immunodeficiency virus (HIV) infection and their role in viral transmission in the immune response were studied in detail. We observed that highly purified cultured DCs were infected with the T-tropic Lai strain of HIV type 1 (HIV-1Lai) via...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.7.4544-4547.1995
更新日期:1995-07-01 00:00:00
abstract::Worldwide amphibian population declines have been ascribed to global warming, increasing pollution levels, and other factors directly related to human activities. These factors may additionally be favoring the emergence of novel pathogens. In this report, we have determined the complete genome sequence of the emerging...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.07108-11
更新日期:2012-04-01 00:00:00
abstract::We established previously that the temperature-dependent host range mutant, td CE 3, of vesicular stomatitis virus (VSV) New Jersey possesses temperature-sensitive RNA transcriptase activity. In this paper, we describe dissociation and reconstitution experiments designed to determine which VSV polypeptide is affected ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.22.2.381-388.1977
更新日期:1977-05-01 00:00:00
abstract:UNLABELLED:Of the various genetic subtypes of human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2) and simian immunodeficiency virus (SIV), only in subtype C of HIV-1 is a genetically variant NF-κB binding site found at the core of the viral promoter in association with a subtype-specific Sp1III motif. How the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00308-16
更新日期:2016-07-27 00:00:00
abstract::Inoculation of 3-day-old (3D) or 3-week-old (3W) ducklings with duck hepatitis B virus results in chronic or transient infection, respectively. We previously showed that rapid production of neutralizing antibody following inoculation of 3W ducklings prevents virus from spreading in the liver and leads to a transient i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.15.9896-9903.2005
更新日期:2005-08-01 00:00:00
abstract::Natural killer T (NKT) cells are unique T lymphocytes that recognize CD1d-bound lipid antigens and play an important role in both innate and acquired immune responses against infectious diseases and tumors. We have already shown that a vesicular stomatitis virus (VSV) infection results in the rapid inhibition of murin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00881-08
更新日期:2008-12-01 00:00:00
abstract::Therapeutic vaccination has the potential to boost immune responses and enhance viral control during chronic infections. However, many therapeutic vaccination approaches have fallen short of expectations, and effective boosting of antiviral T-cell responses is not always observed. To examine these issues, we studied t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.14.8960-8968.2005
更新日期:2005-07-01 00:00:00
abstract::Vaccinia virus (VACV) stimulates long-term immunity against highly pathogenic orthopoxvirus infection of humans (smallpox) and mice (mousepox [ectromelia virus {ECTV}]) despite the lack of a natural host-pathogen relationship with either of these species. Previous research revealed that VACV is able to induce polyfunc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00038-12
更新日期:2012-07-01 00:00:00
abstract::Japanese encephalitis virus (JEV) is a mosquito-borne pathogenic flavivirus responsible for acute viral encephalitis in humans. The cellular entry of JEV is poorly characterized in terms of molecular requirements and pathways. Here we present a systematic study of the internalization mechanism of JEV in fibroblasts an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01399-12
更新日期:2013-01-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) DNA and RNA have been detected in peripheral nervous system (PNS) and central nervous system (CNS) tissues of latently infected mice. However, explant methods are successful in reactivating HSV-1 only from latently infected PNS tissues. In this report, latent herpesvirus infections ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.3.749-756.1988
更新日期:1988-03-01 00:00:00
abstract::Foamy viruses (FVs) generate their Pol protein precursor molecule independently of the Gag protein from a spliced mRNA. This mode of expression raises the question of the mechanism of Pol protein incorporation into the viral particle (capsid). We previously showed that the packaging of (pre)genomic RNA is essential fo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.11.7005-7013.2005
更新日期:2005-06-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) utilizes clathrin-mediated endocytosis for its infectious entry into human foreskin fibroblast (HFF) cells (S. M. Akula, P. P. Naranatt, N.-S. Walia, F.-Z. Wang, B. Fegley, and B. Chandran, J. Virol. 77:7978-7990, 2003). Here, we characterized KSHV entry into primary huma...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02498-08
更新日期:2009-05-01 00:00:00
abstract::The Gag-Pol polyprotein of human immunodeficiency virus type 1 (HIV-1) is not required for efficient viral particle production. However, premature termination codons in pol, particularly in the integrase (IN)-coding region, can markedly impair HIV-1 particle formation, apparently due to the premature activation of the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02329-10
更新日期:2011-04-01 00:00:00
abstract::3-O-(3',3'-dimethylsuccinyl)betulinic acid (PA-457 or bevirimat) potently inhibits human immunodeficiency virus type 1 (HIV-1) maturation by blocking a late step in the Gag processing pathway, specifically the cleavage of SP1 from the C terminus of capsid (CA). To gain insights into the mechanism(s) by which HIV-1 cou...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01369-06
更新日期:2006-11-01 00:00:00
abstract::A cell model of primary macrophages isolated from the peritoneal cavity of flavivirus-susceptible and congenic resistant mice has been used to study the extent and kinetics of antiviral effects against West Nile virus upon priming with alpha/beta interferon (IFN-alpha/beta) or poly(I-C) (pIC). The pattern of flaviviru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.3.1753-1764.2005
更新日期:2005-02-01 00:00:00
abstract::Herpesviruses encode an essential, maturational serine protease whose catalytic domain, assemblin (28 kDa), is released by self-cleavage from a 74-kDa precursor (pPR, pUL80a). Although there is considerable information about the structure and enzymatic characteristics of assemblin, a potential pharmacologic target, co...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02821-06
更新日期:2007-04-01 00:00:00
abstract::White spot syndrome virus (WSSV) occurs worldwide and causes high mortality and considerable economic damage to the shrimp farming industry. No adequate treatments against this virus are available. It is generally accepted that invertebrates such as shrimp do not have an adaptive immune response system such as that pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.4.2057-2061.2004
更新日期:2004-02-01 00:00:00
abstract::The nucleotide sequence was determined for the hemagglutinin gene of the Hong Kong subtype influenza strain A/Bangkok/1/79. The amino acid sequence predicted from these data shows a total of 36 amino acid changes as compared with hemagglutinin for a 1968 Hong Kong strain, 11 more than had occurred in a 1975 strain. Th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.3.663-672.1981
更新日期:1981-09-01 00:00:00
abstract::Adult T-cell leukemia (ATL) is caused by human T-cell leukemia virus type 1 (HTLV-1). Elevated expression of vascular endothelial growth factor (VEGF) in ATL patients is associated with leukemic cell invasion and infiltration in different organs. The regulatory protein Tax 1 encoded by HTLV-1 plays a pivotal role in T...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02166-09
更新日期:2010-05-01 00:00:00
abstract::Human cytomegalovirus UL25 codes for a structural phosphoprotein of 85 kDa (C. J. Baldick and T. Shenk, J. Virol. 70:6097-6105, 1996; M. C. Battista et al., J. Virol. 73:3800-3809, 1999). In this study we analyzed the intracellular and intraviral localization of pUL25 by confocal and immunoelectron microscopy and foun...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.7.6073-6075.1999
更新日期:1999-07-01 00:00:00
abstract::Molecularly cloned proviral DNA of avian oncogenic retrovirus CMII was isolated by screening a genomic library of a CMII-transformed quail cell line with a myc-specific probe. On a 10.4-kilobase EcoRI fragment, the cloned DNA contained 4.4 kilobases of CMII proviral sequences extending from the 5' long terminal repeat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.2.576-585.1985
更新日期:1985-05-01 00:00:00
abstract::To identify retroviral antigenic determinants recognized by CD4+ T helper cells during tumor rejection, we established four noncytolytic, helper-type, CD4+ T-cell clones by limiting dilution cultures of mixed lymphocyte-tumor cultures from mice immune to a Friend virus-induced tumor, FBL-3. Among these, three T helper...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.8.4533-4542.1993
更新日期:1993-08-01 00:00:00
abstract::RNA structures play key roles in the replication of RNA viruses. Sequence alignment software, thermodynamic RNA folding programs, and classical comparative phylogenetic analysis were used to build models of six RNA elements in the coding region of the hepatitis C virus (HCV) RNA-dependent RNA polymerase, NS5B. The imp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.3.1352-1366.2004
更新日期:2004-02-01 00:00:00
abstract::The feline immunodeficiency virus (FIV) cat model is extensively used to investigate possible vaccination approaches against AIDS in humans. Although consistent levels of protection have been achieved with FIV, as with other model systems, by immunizing with whole inactivated virus or fixed infected cells, the mechani...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.1.1-10.1999
更新日期:1999-01-01 00:00:00
abstract::Longitudinal studies of T cell immune responses during viral infections in humans are essential for our understanding of how effector T cell responses develop, clear infection, and provide long-lasting immunity. Here, following an outbreak of a Puumala hantavirus infection in the human population, we longitudinally an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05548-11
更新日期:2011-10-01 00:00:00
abstract::African swine fever virus (ASFV) multigene family 360 and 530 (MGF360/530) genes affect viral growth in macrophage cell cultures and virulence in pigs (L. Zsak, Z. Lu, T. G. Burrage, J. G. Neilan, G. F. Kutish, D. M. Moore, and D. L. Rock, J. Virol. 75:3066-3076, 2001). The mechanism by which these novel genes affect ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.4.1858-1864.2004
更新日期:2004-02-01 00:00:00