Abstract:
:Herpes simplex virus type 1 (HSV-1) DNA and RNA have been detected in peripheral nervous system (PNS) and central nervous system (CNS) tissues of latently infected mice. However, explant methods are successful in reactivating HSV-1 only from latently infected PNS tissues. In this report, latent herpesvirus infections in mouse PNS and CNS tissues were compared by in situ hybridization to determine whether the difference in reactivation was at the level of the virus or the host tissue. It was demonstrated that the HSV-1 transcripts present during latency in the mouse PNS and CNS originated from the same region of the genome, the repeats which bracket the long unique sequence. Therefore, the difference in reactivation with PNS and CNS tissues cannot be accounted for by differences in the extent of the HSV-1 genome transcribed during herpesvirus latency. Latent HSV-1 RNA was detected in the trigeminal ganglia (PNS) and the trigeminal system in the CNS from the mesencephalon to the spinal cord as well as other regions of the CNS not noted previously. Latent HSV-1 RNA was found predominantly in neurons but also in a small number of cells which could not be identified as neuronal cells. It is suggested that host differences in CNS and PNS tissues, rather than differences in latent virus transcription, may be important determinants in the HSV-1 reactivation process in explanted tissues.
journal_name
J Viroljournal_title
Journal of virologyauthors
Deatly AM,Spivack JG,Lavi E,O'Boyle DR 2nd,Fraser NWdoi
10.1128/JVI.62.3.749-756.1988subject
Has Abstractpub_date
1988-03-01 00:00:00pages
749-56issue
3eissn
0022-538Xissn
1098-5514journal_volume
62pub_type
杂志文章abstract::Herpes simplex viruses 1 and 2 (HSV-1 and HSV-2) infect and establish latency in peripheral neurons, from which they can reactivate to cause recurrent disease throughout the life of the host. Stress is associated with the exacerbation of clinical symptoms and the induction of recurrences in humans and animal models. T...
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abstract::Identifying protease cleavage sites contributes to our understanding of their specificity and biochemical properties and can help in designing specific inhibitors. One route to this end is the generation and screening of random libraries of cleavage sites. Both synthetic and phage-displayed libraries have been extensi...
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.7.3530-3537.1991
更新日期:1991-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-01-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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doi:10.1128/JVI.9.3.503-509.1972
更新日期:1972-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.3.585-598.1975
更新日期:1975-03-01 00:00:00
abstract::CM2 is the second membrane protein of the influenza C virus and has been demonstrated to play a role in the uncoating and genome packaging processes in influenza C virus replication. Although the effects of N-linked glycosylation, disulfide-linked oligomerization, and palmitoylation of CM2 on virus replication have be...
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更新日期:2020-03-17 00:00:00
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abstract::Betanodaviruses, the causal agents of viral nervous necrosis in marine fish, have bipartite positive-sense RNAs as genomes. The larger genomic segment, RNA1 (3.1 kb), encodes an RNA-dependent RNA polymerase, and the smaller genomic segment, RNA2 (1.4 kb), codes for the coat protein. Betanodaviruses have marked host sp...
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更新日期:2004-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.43.3.1072-1081.1982
更新日期:1982-09-01 00:00:00
abstract::In previous reports it was demonstrated that the Nipah virus V and W proteins have interferon (IFN) antagonist activity due to their ability to block signaling from the IFN-alpha/beta receptor (J. J. Rodriguez, J. P. Parisien, and C. M. Horvath, J. Virol. 76:11476-11483, 2002; M. S. Park et al., J. Virol. 77:1501-1511...
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doi:10.1128/JVI.73.5.3854-3865.1999
更新日期:1999-05-01 00:00:00
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doi:10.1128/JVI.72.4.3451-3454.1998
更新日期:1998-04-01 00:00:00
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doi:10.1128/JVI.61.6.1781-1787.1987
更新日期:1987-06-01 00:00:00
abstract:UNLABELLED:Oncolytic viruses (OVs) are attractive avenues of cancer therapy due to the absence of toxic side effects often seen with current treatment modalities. Bovine herpesvirus 1 (BHV-1) is a species-specific virus that does not induce cytotoxicity in normal primary human cells but can infect and kill various huma...
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更新日期:2014-06-01 00:00:00
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journal_title:Journal of virology
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abstract:UNLABELLED:Epstein-Barr virus (EBV) is a well-established B-cell-tropic virus associated with various lymphoproliferative diseases of both B-cell and non-B-cell origin. EBV is associated with a number of T-cell lymphomas; however, in vitro studies utilizing prototypical EBV type 1 (EBV-1) laboratory strains have genera...
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更新日期:1980-08-01 00:00:00
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更新日期:1993-09-01 00:00:00