p21 of Kirsten murine sarcoma virus is thermolabile in a viral mutant temperature sensitive for the maintenance of transformation.

abstract：:Vesicular stomatitis virus (VSV) has been shown in laboratory studies to be effective against a variety of tumors, including malignant brain tumors. However, attenuation of VSV may be necessary to balance the potential toxicity toward normal cells, particularly when targeting brain tumors. Here we compared 10 recombin...

journal_title：Journal of virology

authors： Wollmann G,Rogulin V,Simon I,Rose JK,van den Pol AN

更新日期：2010-02-01 00:00:00

abstract：:Recombinant vaccinia viruses containing the 22-kilodalton protein (matrixlike or 22K protein) or phosphoprotein gene from respiratory syncytial virus were constructed. These recombinant viruses expressed proteins which were immunoprecipitated by appropriate respiratory syncytial virus antibodies and comigrated with au...

journal_title：Journal of virology

authors： Openshaw PJ,Anderson K,Wertz GW,Askonas BA

更新日期：1990-04-01 00:00:00

abstract：:The required activities of CD4(+) T cells and antibody against the virally encoded oncoprotein simian virus 40 (SV40) Tag have previously been demonstrated by our laboratory to be mediators in achieving antitumor responses and tumor protection through antibody-dependent cell-mediated cytotoxicity (ADCC). In this study...

journal_title：Journal of virology

authors： Lowe DB,Shearer MH,Jumper CA,Bright RK,Kennedy RC

更新日期：2010-01-01 00:00:00

abstract：:We introduced various mutations into the activation and RNA binding domains of human immunodeficiency virus type 1 (HIV-1) Tat in order to develop a novel and potent transdominant Tat protein and to characterize its mechanism of action. The different mutant Tat proteins were characterized for their abilities to activa...

journal_title：Journal of virology

authors： Orsini MJ,Debouck CM

更新日期：1996-11-01 00:00:00

abstract：:We previously reported that exosomal transfer of hepatitis C virus (HCV) positive-strand RNA from human Huh-7 hepatoma cells to human plasmacytoid dendritic cells (pDCs) triggers pDC alpha/beta interferon (IFN-α/β) production in a Toll-like receptor 7 (TLR7)-dependent, virus-independent manner. Here we show that human...

journal_title：Journal of virology

authors： Wieland SF,Takahashi K,Boyd B,Whitten-Bauer C,Ngo N,de la Torre JC,Chisari FV

更新日期：2014-01-01 00:00:00

abstract：:We have established a genetic assay for the multimerization of retroviral gag polyproteins. This assay is based on the GAL4 two-hybrid system for studying protein-protein interactions (S. Fields and O. Song, Nature (London) 340:245-246, 1989). In our initial experiments, we generated Saccharomyces cerevisiae plasmids ...

journal_title：Journal of virology

authors： Luban J,Alin KB,Bossolt KL,Humaran T,Goff SP

更新日期：1992-08-01 00:00:00

abstract：:The identification of several simian immunodeficiency virus mac251 (SIV(mac251)) cytotoxic T-lymphocyte epitopes recognized by CD8(+) T cells of infected rhesus macaques carrying the Mamu-A*01 molecule and the use of peptide-major histocompatibility complex tetrameric complexes enable the study of the frequency, bread...

journal_title：Journal of virology

authors： Hel Z,Nacsa J,Kelsall B,Tsai WP,Letvin N,Parks RW,Tryniszewska E,Picker L,Lewis MG,Edghill-Smith Y,Moniuszko M,Pal R,Stevceva L,Altman JD,Allen TM,Watkins D,Torres JV,Berzofsky JA,Belyakov IM,Strober W,Franchini G

更新日期：2001-12-01 00:00:00

abstract：:Previous work by our laboratory and others has shown that mouse cells normally resistant to tumor necrosis factor can be made sensitive to the cytokine by the expression of adenovirus E1A. The E1A gene can be introduced by either infection or transfection, and either of the two major E1A proteins, 289R or 243R, can in...

journal_title：Journal of virology

authors： Duerksen-Hughes PJ,Hermiston TW,Wold WS,Gooding LR

更新日期：1991-03-01 00:00:00

abstract：:Epstein-Barr virus (EBV) is a ubiquitous human pathogen associated with Burkitt's lymphoma and nasopharyngeal carcinoma. Although the EBV genome harbors more than a hundred genes, a full transcription map with EBV polyadenylation profiles remains unknown. To elucidate the 3' ends of all EBV transcripts genome-wide, we...

journal_title：Journal of virology

authors： Majerciak V,Yang W,Zheng J,Zhu J,Zheng ZM

更新日期：2019-01-04 00:00:00

abstract：:Alternative splicing of adeno-associated virus type 2 (AAV2) P19-generated pre-mRNAs generates the small Rep proteins Rep52 and Rep40, which differ in their carboxyl termini. Both proteins are required for optimal packaging of AAV2 genomes. AAV5 Rep-encoding P19-generated transcripts are primarily polyadenylated withi...

journal_title：Journal of virology

authors： Farris KD,Pintel DJ

更新日期：2010-01-01 00:00:00

abstract：:Mitochondria are important for the viral life cycle, mainly by providing the energy required for viral replication and assembly. A highly complex interaction with mitochondria is exerted by rubella virus (RV), which includes an increase in the mitochondrial membrane potential as a general marker for mitochondrial acti...

journal_title：Journal of virology

authors： Claus C,Schönefeld K,Hübner D,Chey S,Reibetanz U,Liebert UG

更新日期：2013-08-01 00:00:00

abstract：:Purified rabies virions, unlabeled or labeled with radioactive amino acids or d-glucosamine, were dissociated into their polypeptides by treatment with sodium dodecyl sulfate in a reducing environment and fractionated by electroiphoresis in sodium dodecyl sulfate-containing polyacrylamide gel. The molecular weights of...

journal_title：Journal of virology

authors： Sokol F,Stancek D,Koprowski H

更新日期：1971-02-01 00:00:00

abstract：:Although recombinant human adenovirus (HAV)-based vectors offer several advantages for somatic gene therapy and vaccination over other viral vectors, it would be desirable to develop alternative vectors with prolonged expression and decreased toxicity. Toward this objective, a replication-defective bovine adenovirus t...

journal_title：Journal of virology

authors： Reddy PS,Idamakanti N,Chen Y,Whale T,Babiuk LA,Mehtali M,Tikoo SK

更新日期：1999-11-01 00:00:00

abstract：:Naked DNA vaccines expressing the prM and E genes of two tick-borne flaviviruses, Russian spring summer encephalitis (RSSE) virus and Central European encephalitis (CEE) virus were evaluated in mice. The vaccines were administered by particle bombardment of DNA-coated gold beads by Accell gene gun inoculation. Two imm...

journal_title：Journal of virology

authors： Schmaljohn C,Vanderzanden L,Bray M,Custer D,Meyer B,Li D,Rossi C,Fuller D,Fuller J,Haynes J,Huggins J

更新日期：1997-12-01 00:00:00

abstract：:Three hypervariable regions were identified in a central portion of open reading frame 1 of TT virus DNA, which codes for a putative capsid protein of 770 amino acids. TT virus circulates as quasispecies, with many amino acid substitutions in hypervariable regions, to evade immune surveillance of the hosts and to esta...

journal_title：Journal of virology

authors： Nishizawa T,Okamoto H,Tsuda F,Aikawa T,Sugai Y,Konishi K,Akahane Y,Ukita M,Tanaka T,Miyakawa Y,Mayumi M

更新日期：1999-11-01 00:00:00

abstract：:Disulfide bonds reportedly stabilize the capsids of several viruses, including papillomavirus, polyomavirus, and simian virus 40, and have been detected in herpes simplex virus (HSV) capsids. In this study, we show that in mature HSV-1 virions, capsid proteins VP5, VP23, VP19C, UL17, and UL25 participate in covalent c...

journal_title：Journal of virology

authors： Szczepaniak R,Nellissery J,Jadwin JA,Makhov AM,Kosinski A,Conway JF,Weller SK

更新日期：2011-09-01 00:00:00

abstract：:Human metapneumovirus (HMPV) has recently been identified as a significant cause of serious respiratory tract disease in humans. In particular, the emerging information on the contribution of HMPV to pediatric respiratory tract disease suggests that it will be important to develop a vaccine against this virus for use ...

journal_title：Journal of virology

authors： Biacchesi S,Skiadopoulos MH,Yang L,Lamirande EW,Tran KC,Murphy BR,Collins PL,Buchholz UJ

更新日期：2004-12-01 00:00:00

abstract：:Human norovirus (HuNoV) is a major causative agent of food-borne gastroenteritis worldwide. Currently, there are no vaccines or effective therapeutic interventions for this virus. Development of an attenuated vaccine for HuNoV has been hampered by the inability to grow the virus in cell culture. Thus, a vector-based v...

journal_title：Journal of virology

authors： Ma Y,Li J

更新日期：2011-03-01 00:00:00

abstract：:Human papillomavirus type 16 (HPV16) is the primary etiologic agent for cervical cancer. The infectious entry of HPV16 into cells occurs via a so-far poorly characterized clathrin- and caveolin-independent endocytic pathway, which involves tetraspanin proteins and actin. In this study, we investigated the specific rol...

journal_title：Journal of virology

authors： Scheffer KD,Gawlitza A,Spoden GA,Zhang XA,Lambert C,Berditchevski F,Florin L

更新日期：2013-03-01 00:00:00

abstract：:Injection of conventional or axenic weanling mice with potent sheep or goat antibody to mouse interferon alpha/beta resulted in a decrease in the basal level of 2-5A synthetase in resting peritoneal macrophages and rendered these cells permissive for vesicular stomatitis virus. There was a good inverse correlation bet...

journal_title：Journal of virology

authors： Gresser I,Vignaux F,Belardelli F,Tovey MG,Maunoury MT

更新日期：1985-01-01 00:00:00

abstract：:ETnII elements are mobile members of the repetitive early transposon family of mouse long terminal repeat (LTR) retroelements and have caused a number of mutations by inserting into genes. ETnII sequences lack retroviral genes, but the recent discovery of related MusD retroviral elements with regions similar to gag, p...

journal_title：Journal of virology

authors： Baust C,Gagnier L,Baillie GJ,Harris MJ,Juriloff DM,Mager DL

更新日期：2003-11-01 00:00:00

abstract：:The hepatitis E virus (HEV), a nonenveloped RNA virus, is the causative agent of hepatitis E. The mode by which HEV attaches to and enters into target cells for productive infection remains unidentified. Open reading frame 2 (ORF2) of HEV encodes its major capsid protein, pORF2, which is likely to have the determinant...

journal_title：Journal of virology

authors： Kalia M,Chandra V,Rahman SA,Sehgal D,Jameel S

更新日期：2009-12-01 00:00:00

abstract：:Mason-Pfizer monkey virus 60-70S RNA has a molecular weight of 8 times 10-6 when analyzed on polyacrylamide gels. Dissociation of 60-70S RNA of Mason-Pfizer monkey virus and murine leukemia virus by heat or formamide (40%) resulted in conversion to identical subunit structures of 2.8 times 10-6 daltons; treatment with...

journal_title：Journal of virology

authors： Schochetman G,Schlom J

更新日期：1975-02-01 00:00:00

abstract：:Woodchuck hepatitis virus (WHV) mutants with core internal deletions (CID) occur naturally in chronically WHV-infected woodchucks, as do hepatitis B virus mutants in humans. We studied the replication of WHV deletion mutants in primary woodchuck hepatocyte cultures and in vivo after transmission to naive woodchucks. B...

journal_title：Journal of virology

authors： Lu M,Hilken G,Yang D,Kemper T,Roggendorf M

更新日期：2001-04-01 00:00:00

abstract：:ICP0, an alpha (immediate-early) protein of herpes simplex virus 1, performs at least two key functions. It blocks inhibition of viral-gene expression by interferon, a function dependent on the degradation of the ND10 components PML and SP100 by the ubiquitin ligase expressed by the RING finger (RF), and it blocks sil...

journal_title：Journal of virology

authors： Gu H,Roizman B

更新日期：2009-01-01 00:00:00

abstract：:Hepatitis C virus (HCV) is a global challenge to public health. Several factors have been proven to be critical for HCV entry, including the newly identified claudin-1 (CLDN1). However, the mechanism of HCV entry is still obscure. Presently, among the 20 members of the claudin family identified in humans so far, CLDN1...

journal_title：Journal of virology

authors： Zheng A,Yuan F,Li Y,Zhu F,Hou P,Li J,Song X,Ding M,Deng H

更新日期：2007-11-01 00:00:00

abstract：:A polypeptide (p40) of approximately 40,000 molecular weight was isolated from herpes simplex virus type 1 and 2 nucleocapsids by gel filtration and ion exchange chromatography. This protein appears to be the same as protein 22a described previously (Gibson and Roizman, J. Virol. 10:1044--1052, 1972). Competition immu...

journal_title：Journal of virology

authors： Heilman CJ Jr,Zweig M,Stephenson JR,Hampar B

更新日期：1979-01-01 00:00:00

abstract：UNLABELLED:The safety and efficacy of the live-attenuated Japanese encephalitis virus (JEV) SA14-14-2 vaccine are attributed to mutations that accumulated in the viral genome during its derivation. However, little is known about the contribution that is made by most of these mutations to virulence attenuation and vacci...

journal_title：Journal of virology

authors： Gromowski GD,Firestone CY,Whitehead SS

更新日期：2015-06-01 00:00:00

abstract：:A number of options are available to modify and improve DNA vaccines. An interesting approach to improve DNA vaccines is to fuse bioactive domains, like cytotoxic-T-lymphocyte-associated protein 4 (CTLA-4), to an antigen. Such fusion antigens are expressed in vivo and directed to immune cells by the specific bioactive...

journal_title：Journal of virology

authors： Lu M,Isogawa M,Xu Y,Hilken G

更新日期：2005-05-01 00:00:00

abstract：:Two chimpanzees immunized with woodchuck hepatitis virus (WHV) surface antigen (WHsAg) developed antibodies cross-reactive with hepatitis B virus (HBV) surface antigen (HBsAg). After challenge with HBV, one animal was completely protected and the other experienced a subclinical infection, without evidence of liver dis...

journal_title：Journal of virology

authors： Cote PJ,Shapiro M,Engle RE,Popper H,Purcell RH,Gerin JL

更新日期：1986-12-01 00:00:00