Abstract:
:Polypeptides synthesized in Newcastle disease virus (NDV)-infected CHO cells in the absence of glycosylation were characterized. Incorporation of either [3H]mannose of [3H]glucosamine into NDV polypeptides was inhibited to greater than 99% by the antibiotic tunicamycin. Under these conditions, infected cells synthesized proteins which comigrated on polyacrylamide gels with the viral L protein, nucleocapsid protein, membrane protein, and a polypeptide with a molecular weight of 55,000 (P55). These cells did not synthesize polypeptides with the size of the hemagglutinin-neuraminidase (HN) protein or the fusion (F0) protein. They did, however, synthesize new polypeptides with molecular weights of 75,000 (P75), 67,000 (P67), and 52,000 (P52). Peptide analysis revealed that P75 was a host cell protein whose synthesis is enhanced by tunicamycin. P67 corresponded to the unglycosylated forms of the glycoproteins were found to be relatively stable in infected cells. P55, previously thought to correspond to the cleaved form of F0, was found to be a unique viral protein which is associated with intracellular nucleocapsid structures.
journal_name
J Viroljournal_title
Journal of virologyauthors
Morrison TG,Simpson Ddoi
10.1128/JVI.36.1.171-180.1980subject
Has Abstractpub_date
1980-10-01 00:00:00pages
171-80issue
1eissn
0022-538Xissn
1098-5514journal_volume
36pub_type
杂志文章abstract::Reovirus encephalitis in mice was used as a model system to investigate astrocyte activation (astrogliosis) following viral infection of the brain. Reovirus infection resulted in astrogliosis, as evidenced by increased expression of glial fibrillary acidic protein (GFAP), and the upregulation of genes that have been p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02027-18
更新日期:2019-05-01 00:00:00
abstract::Herein we demonstrate that chronic simian immunodeficiency virus (SIV) infection induces significant upregulation of the gut-homing marker alpha4beta7 on macaque NK cells, coupled with downregulation of the lymph node-trafficking marker, CCR7. Interestingly, in naïve animals, alpha4beta7 expression was associated with...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01126-10
更新日期:2010-09-01 00:00:00
abstract::Resistance or susceptibility of various mouse strains to central nervous system disease caused by different strains of coronavirus is well known. Data from the present study draw attention to an additional, genetically determined mechanism controlling CV infections. The resistance to A59 and JHM virus (JHMV) associate...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.9.3371-3377.1988
更新日期:1988-09-01 00:00:00
abstract::Current knowledge of human immunodeficiency virus type 1 envelope (Env) glycoprotein structure and function is based on studies of clade B viruses. We present evidence of sequence and structural differences in viral glycoprotein gp120 between clades B and C. In clade C, the C3 region alpha2-helix exhibits high sequenc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01954-06
更新日期:2007-05-01 00:00:00
abstract::A porcine rotavirus (prv), variant 4F, isolated in tissue culture from the feces of a Chinese pig with diarrhea, was found to have become highly pathogenic when passaged in gnotobiotic piglets (J. C. Bridger, B. Burke, G. M. Beards, and U. Desselberger, J. Gen. Virol. 73:3011-3015, 1992). Comparison with the closely r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.853-857.1997
更新日期:1997-01-01 00:00:00
abstract::A adenovirus type 5 host range mutant (hr440) has been isolated which is defective in a splicing event required to generate the middle-sized mRNA from early region 1A. This defect has been ascribed to two adjacent nucleotide changes which lie five and six nucleotides from the 5' splice site for this mRNA (Solnick, Nat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.1.106-113.1982
更新日期:1982-04-01 00:00:00
abstract:UNLABELLED:There are nine subtypes of influenza A virus neuraminidase (NA), N1 to N9. In addition, influenza B virus also contains NA, and there are two influenza virus NA-like molecules, N10 and N11, which were recently identified from bats. Crystal structures for all of these proteins have been solved, with the excep...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00805-14
更新日期:2014-08-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) V3 loop is essential for coreceptor binding and principally determines tropism for the CCR5 and CXCR4 coreceptors. Using the dual-tropic virus HIV-1(R3A), we previously made an extensive panel of V3 deletions and identified subdomains within V3 that could differentially ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01751-08
更新日期:2009-04-01 00:00:00
abstract:UNLABELLED:A bat virus with high phylogenetic relatedness to human mumps virus (MuV) was identified recently at the nucleic acid level. We analyzed the functional activities of the hemagglutinin-neuraminidase (HN) and the fusion (F) proteins of the bat virus (batMuV) and compared them to the respective proteins of a hu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03693-14
更新日期:2015-04-01 00:00:00
abstract::The involvement of the mouse mammary tumor virus (MTV) in spontaneous and hormone-induced mammary tumors in low-mammary-tumor mouse strains was studied by comparing the amounts of MTV RNA and MTV DNA sequences in mammary tumors and other tissues of mice with an without hormonal treatments. The following results were o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.27.3.551-559.1978
更新日期:1978-09-01 00:00:00
abstract::Using gel shift assays, we found that the human immunodeficiency virus type 1 (HIV-1) Tat protein (Tat-1) bound both HIV-1 and HIV-2 TAR RNAs with similar high affinities. In contrast, the HIV-2 Tat protein (Tat-2) bound only TAR-2 RNA with high affinity. We conclude that the weak in vivo activity of Tat-2 on the HIV-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.2.1110-1121.1993
更新日期:1993-02-01 00:00:00
abstract::The Borna disease virus (BDV) is the prototype member of the Bornaviridae, and it replicates in the cell nucleus. The BDV p24P and p40N proteins carry nuclear localization signals (NLS) and are found in the nuclei of infected cells. The BDV p10 protein does not have an NLS, but it binds with P and/or N and is transloc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.7.3413-3417.2000
更新日期:2000-04-01 00:00:00
abstract::The Parvovirus H-1 replicates autonomously in hamster embryo cells. A DNA synthetic event, called HA-DNA synthesis, upon which subsequent viral RNA and viral hemagglutinin synthesis is dependent, is initiated in late S phase of the infected cell (18). It was postulated that HA-DNA represents parental viral replicative...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.2.400-410.1974
更新日期:1974-02-01 00:00:00
abstract::We have previously shown that styrylquinolines (SQLs) are integrase inhibitors in vitro. They compete with the long terminal repeat substrate for integrase. Here, we describe the cellular mode of action of these molecules. We show that SQLs do not interfere with virus entry. In fact, concentrations of up to 20 times t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.11.5728-5736.2004
更新日期:2004-06-01 00:00:00
abstract::To combat emerging coronaviruses, developing safe and efficient platforms to evaluate viral protease activities and the efficacy of protease inhibitors is a high priority. Here, we exploit a biosafety level 2 (BSL-2) chimeric Sindbis virus system to evaluate protease activities and the efficacy of inhibitors directed ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01749-14
更新日期:2014-10-01 00:00:00
abstract::Herpesviruses are important pathogens of humans and other animals. Herpesvirus infectious clones that can reconstitute phenotypically wild-type (wt) virus are extremely valuable tools for elucidating the roles of specific genes in virus pathophysiology as well as for making vaccines. Ictalurid herpesvirus 1 (channel c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02152-07
更新日期:2008-04-01 00:00:00
abstract::The induction of a unique macrophage procoagulant molecule by murine hepatitis virus strain 3 correlates with the severity of viral hepatitis. The role of tyrosine phosphorylation in the signalling pathway leading to procoagulant expression was studied. Murine hepatitis virus strain 3 initiated a rapid increase in pho...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.9.5824-5828.1995
更新日期:1995-09-01 00:00:00
abstract:UNLABELLED:Type I IFN signaling, which is initiated through activation of the alpha interferon receptor (IFNAR), regulates the expression of proteins that are crucial contributors to immune responses. Paramyxoviruses, including human metapneumovirus (HMPV), have evolved mechanisms to inhibit IFNAR signaling, but the sp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03275-14
更新日期:2015-04-01 00:00:00
abstract::Transcription of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) is activated through binding of the viral Tat protein to the trans-activating response (TAR) element at the 5' end of the nascent transcript. Whereas HIV type 1 (HIV-1) TAR folds a simple hairpin structure, the corresponding do...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00530-08
更新日期:2008-09-01 00:00:00
abstract::Herpes B virus (BV) naturally infects macaque monkeys and is genetically similar to herpes simplex virus (HSV). Zoonotic infection of humans can cause encephalitis and if untreated has a fatality rate of ∼80%. The frequent use of macaques in biomedical research emphasizes the need to understand the molecular basis of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00505-11
更新日期:2011-07-01 00:00:00
abstract::Rhesus monkey rhadinovirus (RRV), a simian gamma-2 herpesvirus closely related to the Kaposi sarcoma-associated herpesvirus, replicates lytically in cultured rhesus monkey fibroblasts and establishes persistence in B cells. Overlapping cosmid clones were generated that encompass the entire 130-kilobase-pair genome of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.3.1549-1562.2006
更新日期:2006-02-01 00:00:00
abstract::The role of glycosylation and of disulfide bonds in the formation of the native structure of the Sendai virus hemagglutinin-neuraminidase (HN) and fusion (F0) glycoproteins was studied. In contrast to the HN and F0 synthesized in vivo, the proteins made from pSP6 transcripts in reticulocyte lysates, whether glycosylat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.2.892-900.1989
更新日期:1989-02-01 00:00:00
abstract::Intragastric inoculation with whole-virion vaccine of inactivated influenza virus resulted in production of hemagglutinin (HA)-specific immunoglobulin A (IgA) and IgG both in lung lavage fluids and in serum samples of mice. HA-specific IgA was the predominant isotypic antibody secreted in the lung lavage fluids (avera...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.7.2150-2154.1987
更新日期:1987-07-01 00:00:00
abstract::High-risk human papillomaviruses encode two oncogenes, E6 and E7, expressed in nearly all cervical cancers. Although E7 protein is best known for its ability to inactivate the retinoblastoma tumor suppressor protein, pRb, many other activities for E7 have been proposed in in vitro studies. Herein, we describe studies ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.17.11392-11402.2005
更新日期:2005-09-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) infects alveolar macrophages (AMϕ), causing dysregulated alpha interferon (IFN-α) and tumor necrosis factor alpha (TNF-α) production through a mechanism(s) yet to be resolved. Here, we show that AMϕ infected with PRRSV secreted a reduced quantity of IFN-α fol...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01251-17
更新日期:2018-01-02 00:00:00
abstract::Duck hepatitis B virus mutants containing frameshift or stop codon mutations in a portion of the viral pol gene separating the terminal protein and reverse transcriptase domains had a leaky phenotype and, depending on the location and type of mutation, synthesized up to 10% as much viral DNA as did the wild type. This...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.5.2155-2163.1991
更新日期:1991-05-01 00:00:00
abstract::The human antibody response to flavivirus infection is dominantly directed against a cross-reactive epitope on the fusion loop of domain II (DII-FL) of the envelope (E) protein. Although antibodies against this epitope fail to recognize fully mature West Nile virus (WNV) virions and accordingly neutralize infection po...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05859-11
更新日期:2011-11-01 00:00:00
abstract::The unexpectedly low efficacy of influenza vaccine during school outbreaks of influenza B virus in the spring of 1987 in Japan was probably attributable to a poor antibody response of vaccinees to the epidemic viruses. An antigenic analysis of the causative B viruses isolated in 1987 and 1988 showed much variation in ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.2860-2865.1990
更新日期:1990-06-01 00:00:00
abstract::Rift Valley fever virus strain MP-12 was generated by serial plaque passages of parental strain ZH548 12 times in MRC-5 cells in the presence of a chemical mutagen, 5-fluorouracil. As a result, MP-12 encoded 4, 9, and 10 mutations in the S, M, and L segments, respectively. Among them, mutations in the M and L segments...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02081-12
更新日期:2012-12-01 00:00:00
abstract::Nonstructural protein 1 (NS1) of yellow fever virus (YF) is a glycoprotein localized to extracytoplasmic compartments within infected cells. We have previously shown that NS1 can be supplied in trans and is required for viral RNA replication, a process thought to occur in membrane-bound cytoplasmic complexes. Here we ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.6.4611-4621.1999
更新日期:1999-06-01 00:00:00