Abstract:
:Intragastric inoculation with whole-virion vaccine of inactivated influenza virus resulted in production of hemagglutinin (HA)-specific immunoglobulin A (IgA) and IgG both in lung lavage fluids and in serum samples of mice. HA-specific IgA was the predominant isotypic antibody secreted in the lung lavage fluids (average IgA/IgG ratio, 13:1), whereas HA-specific IgG was the major antibody class in serum (average IgA/IgG ratio, 0.3:1). These responses were similar to the antibody responses stimulated by intranasal infection with live influenza virus. In vitro cultures of lymphoid cells from lungs and Peyer's patches, but not from spleens, in the presence of homologous antigen, from mice vaccinated intragastrically synthesized mostly HA-specific IgA. Mice immunized parenterally with inactivated influenza virus produced only IgG in lung lavage fluids and sera. Cultures of lymphoid cells from their spleens, but not their lungs, synthesized HA-specific IgG upon antigenic stimulation in vitro; neither synthesized IgA. These in vitro cell culture results, as well as the inverse relationship of IgA/IgG ratios in lung lavage fluids and sera, demonstrated that the IgA antibody in lung lavage fluids was actively synthesized locally in the lungs of intragastrically immunized mice. This finding was consistent with the migratory distribution of antigen-primed lymphoid cells from Peyer's patches to distant lymphoid tissue such as lung. Intragastric vaccination conferred protection against intranasal challenge with a lethal dose of virulent virus.
journal_name
J Viroljournal_title
Journal of virologyauthors
Chen KS,Burlington DB,Quinnan GV Jrdoi
10.1128/JVI.61.7.2150-2154.1987subject
Has Abstractpub_date
1987-07-01 00:00:00pages
2150-4issue
7eissn
0022-538Xissn
1098-5514journal_volume
61pub_type
杂志文章abstract::Under natural conditions and in some experimental models, rabies virus infection of the central nervous system causes relatively mild histopathological changes, without prominent evidence of neuronal death despite its lethality. In this study, the effects of rabies virus infection on the structure of neurons were inve...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01677-07
更新日期:2008-01-01 00:00:00
abstract::Herpesviruses use a cascade of interactions with different cell surface molecules to gain entry into cells. In many cases, this involves binding to abundant glycosaminoglycans or integrins followed by interactions with more limited cell surface proteins, leading to fusion with cellular membranes. Human cytomegalovirus...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01623-08
更新日期:2008-12-01 00:00:00
abstract::Gene expression of human immunodeficiency virus (HIV) is modulated by both cellular transcription factors, which bind to cis-acting regulatory elements in the HIV-1 long terminal repeat (LTR) and the viral transactivator, tat. The enhancer element in the HIV-1 LTR which extends from -103 to -82 is critical for gene ex...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.1002-1009.1994
更新日期:1994-02-01 00:00:00
abstract::Highly pathogenic influenza A viruses (IAV) from avian hosts were first reported to directly infect humans 20 years ago. However, such infections are rare events, and our understanding of factors promoting or restricting zoonotic transmission is still limited. One accessory protein of IAV, PB1-F2, was associated with ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00425-18
更新日期:2018-05-14 00:00:00
abstract::By marker rescue with cloned herpes simplex virus 2 DNA fragments, we have mapped the temperature-sensitive mutations of a series of herpes simplex virus 2 mutants to a region of the herpes simplex virus 2 genome that lies within or near the coding sequences for the major DNA-binding protein, ICP8. In cells infected w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.1.332-342.1983
更新日期:1983-01-01 00:00:00
abstract::Broadly neutralizing antibodies have been isolated that bind the glycan shield of the HIV-1 envelope spike. One such antibody, PGT135, contacts the intrinsic mannose patch of gp120 at the Asn332, Asn392, and Asn386 glycosylation sites. Here, site-specific glycosylation analysis of recombinant gp120 revealed glycan mic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00230-15
更新日期:2015-07-01 00:00:00
abstract::We constructed vaccine vectors based on live recombinant vesicular stomatitis virus (VSV) and a Semliki Forest virus (SFV) replicon (SFVG) that propagates through expression of the VSV glycoprotein (G). These vectors expressing simian immunodeficiency virus (SIV) Gag and Env proteins were used to vaccinate rhesus maca...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00342-11
更新日期:2011-06-01 00:00:00
abstract::Sequences and structures within the terminal genomic regions of plus-strand RNA viruses are targets for the binding of host proteins that modulate functions such as translation, RNA replication, and encapsidation. Using murine norovirus 1 (MNV-1), we describe the presence of long-range RNA-RNA interactions that were s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03433-12
更新日期:2013-11-01 00:00:00
abstract::In wild aquatic birds and poultry around the world, influenza A viruses carrying 15 antigenic subtypes of hemagglutinin (HA) and 9 antigenic subtypes of neuraminidase (NA) have been described. Here we describe a previously unidentified antigenic subtype of HA (H16), detected in viruses circulating in black-headed gull...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.5.2814-2822.2005
更新日期:2005-03-01 00:00:00
abstract::Caprine arthritis encephalitis virus (CAEV) is a lentivirus closely related to visna virus and more distantly to other lentiviruses, such as human immunodeficiency virus. The genomes of visna virus and CAEV contain a tat gene encoding a protein able to weakly transactivate its own long terminal repeat, suggesting that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.9.5445-5454.1995
更新日期:1995-09-01 00:00:00
abstract::The genome of varicella-zoster virus (VZV) is a linear, double-stranded molecule of DNA composed of a long (L) region covalently linked to a short (S) region. The S region is capable of inverting relative to a fixed orientation of the L region, giving rise to two equimolar populations. We have investigated other forms...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.1.194-200.1985
更新日期:1985-10-01 00:00:00
abstract::Transfection has been found and characterized in pneumococcus. For replicating omega3 phage DNA extracted from infected cells, transfection was relatively efficient and rose linearly with DNA concentration and quadratically with time, according to T(T - 3.5) min(2). For mature DNA extracted from phage particles, trans...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.1.60-72.1978
更新日期:1978-01-01 00:00:00
abstract::Monoclonal antibodies (MAbs) directed against the E2 glycoprotein of mouse hepatitis virus (MHV) have been classified according to their ability to bind to either of the two purified 90,000-molecular-weight subunits (90K subunits) of the 180K peplomeric glycoprotein E2. Correlation with previously reported information...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.3051-3055.1990
更新日期:1990-06-01 00:00:00
abstract::Adenovirus type 2 (Ad2)-transformed hamster and rat cells are susceptible to lysis by natural killer (NK) cells from the host of origin and are nontumorigenic in immunocompetent hamsters and rats, respectively. These NK-cell-susceptible, virus-transformed cells are, however, highly tumorigenic in athymic (nude) mice--...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.7.2155-2161.1987
更新日期:1987-07-01 00:00:00
abstract::We have investigated the species-specific replication of polyomavirus DNA in the cell-free system that was established previously (Y. Murakami, T. Eki, M. Yamada, C. Prives, and J. Hurwitz, Proc. Natl. Acad. Sci. USA 83:6347-6351, 1986). Extracts from various species of cells supported polyomavirus DNA replication in ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.9.4874-4881.1991
更新日期:1991-09-01 00:00:00
abstract::Rfv3 is an autosomal dominant gene that influences the recovery of resistant mice from Friend retrovirus (FV) infection by limiting viremia and promoting a more potent neutralizing antibody response. We previously reported that Rfv3 is encoded by Apobec3, an innate retrovirus restriction factor. However, it was recent...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01838-10
更新日期:2011-01-01 00:00:00
abstract::The genome of a canine parvovirus isolate strain (CPV-N) was cloned, and the DNA sequence was determined. The entire genome, including ends, was 5,323 nucleotides in length. The terminal repeat at the 3' end of the genome shared similar structural characteristics but limited homology with the rodent parvoviruses. The ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.1.266-276.1988
更新日期:1988-01-01 00:00:00
abstract::When human adenovirus type 2 or 12 infects cells, either productively or non-productively, three classes of viral deoxyribonucleic acid (DNA) are found within the cells: (i) viral DNA which cosediments with DNA extracted from infectious adenovirions at 31.3S for adenovirus type 2 and at 29.0S for adenovirus type 12, (...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.6.707-719.1971
更新日期:1971-06-01 00:00:00
abstract::We have evaluated the potential of conferring protective immunity to herpes simplex virus type 2 (HSV-2) by selectively inducing an HSV-specific CD8(+) cytotoxic T-lymphocyte (CTL) response directed against a single major histocompatibility complex class I-restricted CTL recognition epitope. We generated a recombinant...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.12.9567-9574.1998
更新日期:1998-12-01 00:00:00
abstract::Lymphocyte antigen 6E (LY6E) is a GPI-anchored, interferon-inducible protein that has been shown to modulate viral infection in a cell type-dependent manner. Our recent work showed that LY6E promotes HIV-1 infection in some high-CD4-expressing cells, including human peripheral blood mononuclear cells (PBMCs) and the S...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01866-18
更新日期:2019-03-21 00:00:00
abstract::Parvoviruses infect humans and a broad range of animals, from mammals to crustaceans, and generally are associated with a variety of acute and chronic diseases. However, many others cause persistent infections and are not known to be associated with any disease. Viral persistence is likely related to the ability to in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00828-11
更新日期:2011-10-01 00:00:00
abstract::Pseudomonas aeruginosa myovirus KZ has a 270-kb genome within a T=27 icosahedral capsid that contains a large, unusual, and structurally well-defined protein cylindrical inner body (IB) spanning its interior. Proteolysis forms a pivotal stage in KZ head and IB morphogenesis, with the protease gp175 cleaving at least 1...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01008-13
更新日期:2013-08-01 00:00:00
abstract:UNLABELLED:Kaposi's sarcoma-associated herpesvirus (KSHV, also called human herpesvirus 8) is linked to the development of Kaposi's sarcoma (KS), primary effusion lymphoma (PEL), and multicentric Castleman's disease (MCD). KSHV expresses several proteins that modulate host cell signaling pathways. One of these proteins...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00511-14
更新日期:2014-08-01 00:00:00
abstract::To identify the domains of Vpr that are involved nuclear localization, we transfected HeLa cells with a panel of expression vectors that encode mutant Vpr protein with deletions or substitutions within putative domains. Immunofluorescence staining of transfected cells revealed that wild-type Vpr was localized predomin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.15.7179-7186.2000
更新日期:2000-08-01 00:00:00
abstract::p60src, the transforming protein of Rous sarcoma virus, was found to contain 0.5 to 0.9 mol of total phosphate per mol of polypeptide. The protein is known to be phosphorylated at two sites, a serine in the amino-terminal domain and a tyrosine in the carboxy-terminal domain. Because our indirect analysis suggests that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.3.813-820.1982
更新日期:1982-03-01 00:00:00
abstract::Antibody was raised against purified vaccinia virus RNA polymerase and used to screen a recombinant vaccinia virus-lambda gt11 library. The DNA from several immunopositive clones was shown by Southern hybridization to originate from the vaccinia virus HindIII E fragment. The nucleotide sequence of the RNA polymerase s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.11.5376-5382.1990
更新日期:1990-11-01 00:00:00
abstract::Adenovirus E1B 55K protein cooperates with E1A gene products to induce cell transformation. E1B 55K mediates its effects by binding to and inhibiting the transcriptional activation and growth-suppression functions of the tumor suppressor p53. Previous studies in vivo have suggested that E1B 55K has an active role in r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.3146-3154.1998
更新日期:1998-04-01 00:00:00
abstract::Hepatitis C virus (HCV) replication in infected patients produces large and diverse viral populations, which give rise to drug-resistant and immune escape variants. Here, we analyzed HCV populations during transmission and diversification in longitudinal and cross-sectional samples using 454/Roche pyrosequencing, in t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02271-09
更新日期:2010-06-01 00:00:00
abstract::The emergence in 1997 and continuance today of a highly lethal H5N1 avian influenza virus (AIV) causing human disease has raised concern about an impending pandemic and the need for a vaccine to prepare for such an occurrence. We previously generated an efficacious vesicular stomatitis virus (VSV)-based AIV vaccine ex...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02637-09
更新日期:2010-05-01 00:00:00
abstract::Sensory neurons within trigeminal ganglia (TG) are the primary site for bovine herpesvirus 1 (BHV-1) latency. During latency, viral gene expression is restricted to the latency-related (LR) gene and the open reading frame ORF-E. We previously constructed an LR mutant virus that expresses LR RNA but not any of the know...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01009-09
更新日期:2009-09-01 00:00:00