Glycan Microheterogeneity at the PGT135 Antibody Recognition Site on HIV-1 gp120 Reveals a Molecular Mechanism for Neutralization Resistance.


:Broadly neutralizing antibodies have been isolated that bind the glycan shield of the HIV-1 envelope spike. One such antibody, PGT135, contacts the intrinsic mannose patch of gp120 at the Asn332, Asn392, and Asn386 glycosylation sites. Here, site-specific glycosylation analysis of recombinant gp120 revealed glycan microheterogeneity sufficient to explain the existence of a minor population of virions resistant to PGT135 neutralization. Target microheterogeneity and antibody glycan specificity are therefore important parameters in HIV-1 vaccine design.


J Virol


Journal of virology


Pritchard LK,Spencer DI,Royle L,Vasiljevic S,Krumm SA,Doores KJ,Crispin M




Has Abstract


2015-07-01 00:00:00














  • Triggering of human parainfluenza virus 3 fusion protein (F) by the hemagglutinin-neuraminidase (HN) protein: an HN mutation diminishes the rate of F activation and fusion.

    abstract::For human parainfluenza virus type 3 and many other paramyxoviruses, membrane fusion mediated by the fusion protein (F) has a stringent requirement for the presence of the homotypic hemagglutinin-neuraminidase protein (HN). With the goal of gaining further insight into the role of HN in the fusion process, we develope...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Porotto M,Murrell M,Greengard O,Moscona A

    更新日期:2003-03-01 00:00:00

  • Chromatin-like structure of adeno-associated virus DNA in infected cells.

    abstract::Adeno-associated virus (AAV) is a single-stranded DNA virus which requires adenovirus as a helper for productive infection. We studied whether intracellular AAV DNA in KB cells was present in a chromatin-like structure by digesting infected cell nuclei with micrococcal nuclease. Virus DNA was detected by agarose gel e...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Marcus-Sekura CJ,Carter BJ

    更新日期:1983-10-01 00:00:00

  • HLA class I-driven evolution of human immunodeficiency virus type 1 subtype c proteome: immune escape and viral load.

    abstract::Human immunodeficiency virus type 1 (HIV-1) mutations that confer escape from cytotoxic T-lymphocyte (CTL) recognition can sometimes result in lower viral fitness. These mutations can then revert upon transmission to a new host in the absence of CTL-mediated immune selection pressure restricted by the HLA alleles of t...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Rousseau CM,Daniels MG,Carlson JM,Kadie C,Crawford H,Prendergast A,Matthews P,Payne R,Rolland M,Raugi DN,Maust BS,Learn GH,Nickle DC,Coovadia H,Ndung'u T,Frahm N,Brander C,Walker BD,Goulder PJ,Bhattacharya T,Hecke

    更新日期:2008-07-01 00:00:00

  • Production of high-titer recombinant adeno-associated virus vectors in the absence of helper adenovirus.

    abstract::Recently, efficient and long-term in vivo gene transfer by recombinant adeno-associated virus type 2 (rAAV) vectors has been demonstrated in a variety of tissues. Further improvement in vector titer and purity will expedite this in vivo exploration and provide preclinical information required for use in human gene the...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Xiao X,Li J,Samulski RJ

    更新日期:1998-03-01 00:00:00

  • Molecular evidence for distinct genotypes of monkey B virus (herpesvirus simiae) which are related to the macaque host species.

    abstract::Although monkey B virus (herpesvirus simiae; BV) is common in all macaque species, fatal human infections appear to be associated with exposure to rhesus macaques (Macaca mulatta), suggesting that BV isolates from rhesus monkeys may be more lethal to nonmacaques than are BV strains indigenous to other macaque species....

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Smith AL,Black DH,Eberle R

    更新日期:1998-11-01 00:00:00

  • Zebrafish ISG15 exerts a strong antiviral activity against RNA and DNA viruses and regulates the interferon response.

    abstract::ISG15, a 15-kDa interferon-induced protein that participates in antiviral defenses of mammals, is highly conserved among vertebrates. In fish, as in mammals, viral infection and interferon treatment induce isg15 expression. The two ubiquitin-like domains of ISG15 and the presence of a consensus LRLRGG sequence in the ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Langevin C,van der Aa LM,Houel A,Torhy C,Briolat V,Lunazzi A,Harmache A,Bremont M,Levraud JP,Boudinot P

    更新日期:2013-09-01 00:00:00

  • Reovirus variants selected for resistance to ammonium chloride have mutations in viral outer-capsid protein sigma3.

    abstract::Mammalian reoviruses are internalized into cells by receptor-mediated endocytosis. Within the endocytic compartment, the viral outer capsid undergoes acid-dependent proteolysis resulting in removal of the sigma3 protein and proteolytic cleavage of the mu1/mu1C protein. Ammonium chloride (AC) is a weak base that blocks...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Clark KM,Wetzel JD,Gu Y,Ebert DH,McAbee SA,Stoneman EK,Baer GS,Zhu Y,Wilson GJ,Prasad BV,Dermody TS

    更新日期:2006-01-01 00:00:00

  • Lymph node but not intradermal injection site macrophages are critical for germinal center formation and antibody responses to rabies vaccination.

    abstract:UNLABELLED:Replication-deficient rabies virus (RABV)-based vaccines induce rapid and potent antibody responses via T cell-independent and T cell-dependent mechanisms. To further investigate early events in vaccine-induced antibody responses against RABV infections, we studied the role of macrophages as mediators of RAB...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Lytle AG,Shen S,McGettigan JP

    更新日期:2015-03-01 00:00:00

  • Human T-cell leukemia virus type 2 Rex protein increases stability and promotes nuclear to cytoplasmic transport of gag/pol and env RNAs.

    abstract::The human T-cell leukemia virus (HTLV) Rex protein is essential for efficient expression of the viral structural and enzymatic gene products. In this study, we assessed the role of the HTLV-2 rex gene in viral RNA expression and Gag protein production. Following transfection of human JM4 T cells with wild-type and rex...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Kusuhara K,Anderson M,Pettiford SM,Green PL

    更新日期:1999-10-01 00:00:00

  • Comparison of the genetic recombination rates of human immunodeficiency virus type 1 in macrophages and T cells.

    abstract::Human immunodeficiency virus type 1 (HIV-1) exhibits a high level of genetic variation generated by frequent mutation and genetic recombination during reverse transcription. We have measured HIV-1 recombination rates in T cells in one round of virus replication. It was recently proposed that HIV-1 recombines far more ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Chen J,Rhodes TD,Hu WS

    更新日期:2005-07-01 00:00:00

  • Proteins of polyhedal cytoplasmic deoxyvirus. II. Nucleotide phosphohydrolase activity associated with frog virus 3.

    abstract::A nucleotide phosphohydrolase is firmly associated with a purified polyhedral cytoplasmic deoxyvirus, frog virus 3. This adenosine triphosphatase is distinguishable from known mammalian cell adenosine triphosphatases and from adenosine triphosphatase of an unrelated cytoplasmic replicating virus grown in the same host...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Vilagines R,McAuslan BR

    更新日期:1971-05-01 00:00:00

  • Characterization of defective RNAs derived from RNA 3 of the Fny strain of cucumber mosaic cucumovirus.

    abstract::Two defective RNAs (designated D RNA 3 alpha and D RNA 3 beta) were found to be associated with the Fny strain of cucumber mosaic cucumovirus but not with the Sny strain after serial passages in a tobacco host. The D RNAs were derived from RNA 3 by single, in-frame deletions within the 3a open reading frame. A full-le...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Graves MV,Roossinck MJ

    更新日期:1995-08-01 00:00:00

  • Penetration of the nervous systems of suckling mice by mammalian reoviruses.

    abstract::Penetration of the nervous systems of suckling mice by prototype strains of the three mammalian reovirus serotypes was studied after footpad inoculation of a dose (10(7) PFU) representing 3.5 x 10(3) 50% lethal doses (LD50) for reovirus type 3 Dearing and less than 1 LD50 for reoviruses type 1 Lang and type 2 Jones. T...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Flamand A,Gagner JP,Morrison LA,Fields BN

    更新日期:1991-01-01 00:00:00

  • Epstein-Barr Virus Oncoprotein LMP1 Mediates Epigenetic Changes in Host Gene Expression through PARP1.

    abstract:UNLABELLED:The latent infection of Epstein-Barr virus (EBV) is associated with 1% of human cancer incidence. Poly(ADP-ribosyl)ation (PARylation) is a posttranslational modification catalyzed by poly(ADP-ribose) polymerases (PARPs) that mediate EBV replication during latency. In this study, we detail the mechanisms that...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Martin KA,Lupey LN,Tempera I

    更新日期:2016-09-12 00:00:00

  • Baculovirus VP80 protein and the F-actin cytoskeleton interact and connect the viral replication factory with the nuclear periphery.

    abstract::Recently, we showed that the Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) VP80 protein is essential for the formation of both virion types, budded virus (BV) and occlusion-derived virus (ODV). Deletion of the vp80 gene did not affect assembly of nucleocapsids. However, these nucleocapsids were not ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Marek M,Merten OW,Galibert L,Vlak JM,van Oers MM

    更新日期:2011-06-01 00:00:00

  • A Point Mutation in the Rhesus Rotavirus VP4 Protein Generated through a Rotavirus Reverse Genetics System Attenuates Biliary Atresia in the Murine Model.

    abstract::Rotavirus infection is one of the most common causes of diarrheal illness in humans. In neonatal mice, rhesus rotavirus (RRV) can induce biliary atresia (BA), a disease resulting in inflammatory obstruction of the extrahepatic biliary tract and intrahepatic bile ducts. We previously showed that the amino acid arginine...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Mohanty SK,Donnelly B,Dupree P,Lobeck I,Mowery S,Meller J,McNeal M,Tiao G

    更新日期:2017-07-12 00:00:00

  • Nuclear factor 90 negatively regulates influenza virus replication by interacting with viral nucleoprotein.

    abstract::Interactions between host factors and the viral replication complex play important roles in host adaptation and regulation of influenza virus replication. A cellular protein, nuclear factor 90 (NF90), was copurified with H5N1 viral nucleoprotein (NP) from human cells in which NP was transiently expressed and identifie...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Wang P,Song W,Mok BW,Zhao P,Qin K,Lai A,Smith GJ,Zhang J,Lin T,Guan Y,Chen H

    更新日期:2009-08-01 00:00:00

  • Accumulation of Cytotoxic CD16+ NK Cells in Simian Immunodeficiency Virus-Infected Lymph Nodes Associated with In Situ Differentiation and Functional Anergy.

    abstract:UNLABELLED:Recent evidence suggests that even in treated infections, human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) replication may continue in lymph nodes (LN), serving as a potential virus reservoir. Here we investigated the effects of lentivirus infection on natural killer (NK) cell frequ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Schafer JL,Li H,Evans TI,Estes JD,Reeves RK

    更新日期:2015-07-01 00:00:00

  • Production of infectious human cytomegalovirus virions is inhibited by drugs that disrupt calcium homeostasis in the endoplasmic reticulum.

    abstract::We previously reported that human cytomegalovirus (HCMV) infection induces endoplasmic reticulum (ER) stress, resulting in activation of the unfolded protein response (UPR). Although some normal consequences of UPR activation (e.g., translation attenuation) are detrimental to viral infection, we have previously shown ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Isler JA,Maguire TG,Alwine JC

    更新日期:2005-12-01 00:00:00

  • Effects of febrile temperature on adenoviral infection and replication: implications for viral therapy of cancer.

    abstract::We previously conducted a phase I/II study using arterial infusions of ONYX-015 (dl1520), a replication-selective adenoviral vector, with E1b deleted, for patients with metastatic colorectal cancer. No dose-limiting toxicities occurred, but >90% of the patients experienced fever. The effects of temperature on the repl...

    journal_title:Journal of virology

    pub_type: 临床试验,杂志文章


    authors: Thorne SH,Brooks G,Lee YL,Au T,Eng LF,Reid T

    更新日期:2005-01-01 00:00:00

  • Processing of gag precursor polyprotein of human T-cell leukemia virus type I by virus-encoded protease.

    abstract::The biological activity encoded in the putative protease gene (pro) of human T-cell leukemia virus type I was investigated by using a vaccinia virus expression vector. The 53-kilodalton gag precursor polyprotein was processed into the mature p19, p24, and p15 gag proteins when the gag and protease-coding sequence was ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Nam SH,Kidokoro M,Shida H,Hatanaka M

    更新日期:1988-10-01 00:00:00

  • Tracking the Emergence of Host-Specific Simian Immunodeficiency Virus env and nef Populations Reveals nef Early Adaptation and Convergent Evolution in Brain of Naturally Progressing Rhesus Macaques.

    abstract:UNLABELLED:While a clear understanding of the events leading to successful establishment of host-specific viral populations and productive infection in the central nervous system (CNS) has not yet been reached, the simian immunodeficiency virus (SIV)-infected rhesus macaque provides a powerful model for the study of hu...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Lamers SL,Nolan DJ,Rife BD,Fogel GB,McGrath MS,Burdo TH,Autissier P,Williams KC,Goodenow MM,Salemi M

    更新日期:2015-08-01 00:00:00

  • Evidence for pH-dependent protease activity in the adeno-associated virus capsid.

    abstract::Incubation of highly purified adeno-associated virus (AAV) capsids in vitro at pH 5.5 induced significant autocleavage of capsid proteins at several amino acid positions. No autocleavage was seen at pH 7.5. Examination of other AAV serotypes showed at least two different pH-induced cleavage patterns, suggesting that d...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Salganik M,Venkatakrishnan B,Bennett A,Lins B,Yarbrough J,Muzyczka N,Agbandje-McKenna M,McKenna R

    更新日期:2012-11-01 00:00:00

  • Characterization of a novel close-to-root papillomavirus from a Florida manatee by using multiply primed rolling-circle amplification: Trichechus manatus latirostris papillomavirus type 1.

    abstract::By using an isothermal multiply primed rolling-circle amplification protocol, the complete genomic DNA of a novel papillomavirus was amplified from a skin lesion biopsy of a Florida manatee (Trichechus manatus latirostris), one of the most endangered marine mammals in United States coastal waters. The nucleotide seque...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Rector A,Bossart GD,Ghim SJ,Sundberg JP,Jenson AB,Van Ranst M

    更新日期:2004-11-01 00:00:00

  • Characterization of an extremely basic protein derived from granulosis virus nucleocapsids.

    abstract::Nucleocapsids were isolated from purified enveloped nucleocapsids of Plodia interpunctella granulosis virus by treatment with Nonidet P-40. When analyzed on sodium dodecyl sulfate-polyacrylamide gels, the nucleocapsids consisted of eight polypeptides. One of these, a major component with a molecular weight of 12,500 (...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Tweeten KA,Bulla LA,Consigli RA

    更新日期:1980-02-01 00:00:00

  • Replication-competent bacterial artificial chromosomes of Marek's disease virus: novel tools for generation of molecularly defined herpesvirus vaccines.

    abstract::Marek's disease (MD), a highly infectious disease caused by an oncogenic herpesvirus, is one of the few herpesvirus diseases against which live attenuated vaccines are used as the main strategy for control. We have constructed bacterial artificial chromosomes (BACs) of the CVI988 (Rispens) strain of the virus, the mos...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Petherbridge L,Howes K,Baigent SJ,Sacco MA,Evans S,Osterrieder N,Nair V

    更新日期:2003-08-01 00:00:00

  • Defining the chemokine basis for leukocyte recruitment during viral encephalitis.

    abstract:UNLABELLED:The encephalitic response to viral infection requires local chemokine production and the ensuing recruitment of immune and inflammatory leukocytes. Accordingly, chemokine receptors present themselves as plausible therapeutic targets for drugs aimed at limiting encephalitic responses. However, it remains uncl...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Michlmayr D,McKimmie CS,Pingen M,Haxton B,Mansfield K,Johnson N,Fooks AR,Graham GJ

    更新日期:2014-09-01 00:00:00

  • Ancient adaptive evolution of tetherin shaped the functions of Vpu and Nef in human immunodeficiency virus and primate lentiviruses.

    abstract::Tetherin/BST-2 is a host-encoded protein that restricts a wide diversity of viruses at the stage of virion release. However, viruses have evolved antagonists of Tetherin, including the Vpu and Nef proteins of primate lentiviruses. Like other host genes subject to viral antagonism, primate Tetherin genes have evolved u...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Lim ES,Malik HS,Emerman M

    更新日期:2010-07-01 00:00:00

  • SH3 binding motif 1 in influenza A virus NS1 protein is essential for PI3K/Akt signaling pathway activation.

    abstract::Recent studies have demonstrated that influenza A virus infection activates the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway by binding of influenza NS1 protein to the p85 regulatory subunit of PI3K. Our previous study proposed that two polyproline motifs in NS1 (amino acids 164 to 167 [PXXP], SH3 bindin...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Shin YK,Li Y,Liu Q,Anderson DH,Babiuk LA,Zhou Y

    更新日期:2007-12-01 00:00:00

  • Recognition of vaccinia virus-infected cells by human natural killer cells depends on natural cytotoxicity receptors.

    abstract::Natural Killer (NK) cells are important in the immune response to a number of viruses; however, the mechanisms used by NK cells to discriminate between healthy and virus-infected cells are only beginning to be understood. Infection with vaccinia virus provokes a marked increase in the susceptibility of target cells to...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Chisholm SE,Reyburn HT

    更新日期:2006-03-01 00:00:00