Abstract：
:A number of herpes simplex virus (HSV) glycoproteins are found in oligomeric states: glycoprotein E (gE)-gI and gH-gL form heterodimers, and both gB and gC have been detected as homodimers. We have further explored the organization of glycoproteins in the virion envelope by using both purified virions to quantitate glycoprotein amounts and proportions and chemical cross-linkers to detect oligomers. We purified gB, gC, gD, and gH from cells infected with HSV type 1 and used these as immunological standards. Glycoproteins present in sucrose gradient-purified preparations of two strains of HSV type 1, KOS and NS, were detected with antibodies to each of the purified proteins. From these data, glycoprotein molar ratios of 1:2:11:16 and 1:1:14:9 were calculated for gB/gC/gD/gH in KOS and NS, respectively. gL was also detected in virions, although we lacked a purified gL standard for quantitation. We then asked whether complexes of these glycoproteins could be identified, and if they existed as homo- or hetero-oligomers. Purified KOS was incubated at 4 degrees C with bis (sulfosuccinimidyl) suberate (BS3), an 11.4 A (1A = 0.1 mm) noncleavable, water-soluble cross-linker. Virus extracts were examined by Western blotting (immunoblotting), or immunoprecipitation followed by Western blotting, to assay for homo- and hetero-oligomers. Homodimers of gB, gC, and gD were detected, and hetero-oligomers containing gB cross-linked to gC, gC to gD, and gD to gB were also identified. gH and gL were detected as a hetero-oligomeric pair and could be cross-linked to gD or gC but not to gB. We conclude that these glycoproteins are capable of forming associations with one another. These studies suggest that glycoproteins are closely associated in virions and have the potential to function as oligomeric complexes.
journal_name
J Viroljournal_title
Journal of virologyauthors
Handler CG,Eisenberg RJ,Cohen GHdoi
10.1128/JVI.70.9.6067-6070.1996subject
Has Abstractpub_date
1996-09-01 00:00:00pages
6067-70issue
9eissn
0022-538Xissn
1098-5514journal_volume
70pub_type
杂志文章abstract：UNLABELLED:Cell culture (cc)-derived hepatitis B virus (HBV) can infect differentiated HepaRG cells, but efficient infection requires addition of polyethylene glycol (PEG) during inoculation. Identification of sodium taurocholate cotransporting polypeptide (NTCP) as an HBV receptor enabled ccHBV infection of NTCP recon...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.01153-16
更新日期：2016-08-26 00:00:00
abstract：:Infection of cells with herpes simplex virus type 1 (HSV-1) results in a rapid alteration of phosphorylation on the large subunit of cellular RNA polymerase II (RNAP II), most likely on its C-terminal domain (S. A. Rice, M. C. Long, V. Lam, C. A. Spencer, J. Virol. 68:988-1001, 1994). This phosphorylation modification...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.69.9.5550-5559.1995
更新日期：1995-09-01 00:00:00
abstract：:Replication of the plus-stranded RNA genome of hepatitis C virus (HCV) occurs in a membrane-bound replication complex consisting of viral and cellular proteins and viral RNA. NS5B, the RNA polymerase of HCV, is anchored to the membranes via a C-terminal 20-amino-acid-long hydrophobic domain, which is flanked on each s...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.02072-05
更新日期：2006-11-01 00:00:00
abstract：:The precise roles of gamma interferon-inducible immunoproteasome-associated molecules in generation of cytotoxic T-lymphocyte (CTL) epitopes have yet to be fully elucidated. We describe here a unique epitope derived from the Epstein-Barr virus (EBV) latent membrane protein 2A (LMP2A) presented by HLA-A*2402 molecules....
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.80.2.883-890.2006
更新日期：2006-01-01 00:00:00
abstract：:The large-particle fraction from the cytoplasm of chick embryo fibroblasts infected with Semliki Forest virus was found to catalyze the incorporation of the 5'-triphosphates of guanosine, adenine, cytidine, and uridine into an acid-insoluble alkali-labile product. The conditions affecting the preparation and assay of ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.1.1.97-109.1967
更新日期：1967-02-01 00:00:00
abstract：:Immune responses to vector-corrected cells have limited the application of gene therapy for treatment of chronic disorders such as inherited deficiency states. We have found that recombinant adeno-associated virus (AAV) efficiently transduces muscle fibers in vivo without activation of cellular and humoral immunity to...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.72.5.4212-4223.1998
更新日期：1998-05-01 00:00:00
abstract：:Host cell interactions of human adenovirus serotypes belonging to subgroups B (adenovirus type 3 [Ad3] and Ad7) and C (Ad2 and Ad5) were comparatively analyzed at three levels: (i) binding of virus particles with host cell receptors; (ii) cointernalization of macromolecules with adenovirions; and (iii) adenovirus-indu...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.64.8.3661-3673.1990
更新日期：1990-08-01 00:00:00
abstract：:The covalent linkage of oncornavirus-specific DNA to chicken DNA was investigated in normal chicken embryo fibroblasts (CEF) and in virus-producing leukemic cells transformed by avian myeloblastosis virus (AMV). The virus-specific sequences present in cellular DNA fractionated by different methods were detected by DNA...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.12.4.721-732.1973
更新日期：1973-10-01 00:00:00
abstract：:A virus structurally similar to viruses associated with maedi, progressive pneumonia, and visna of sheep has been isolated from buffy coat cells of cattle with chronic lymphocytosis. Electron microscope studies revealed three variants of the virion: (i) an intracytoplasmic form 98 to 116 nm in diameter when occurring ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.13.1.197-204.1974
更新日期：1974-01-01 00:00:00
abstract：:Measles virus has two glycoproteins. The larger glycoprotein (HA) is composed of 76,000-dalton subunits that are bound by disulfide bonds. The smaller glycoprotein (F) appears to contain a glucosamine-rich portion that is linked to an unglycosylated protein by disulfide bonds. ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.25.2.687-692.1978
更新日期：1978-02-01 00:00:00
abstract：:Here, we investigated the pre-steady-state deoxynucleoside triphosphate (dNTP) incorporation kinetics of primate foamy virus (PFV) reverse transcriptase (RT) in comparison with those of HIV-1 and MuLV RTs. PFV RT displayed a drastic reduction in primer extension at low dNTP concentrations where HIV-1 RT remains highly...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00088-08
更新日期：2008-08-01 00:00:00
abstract：:Several independent cell lines transformed by simian virus 40 carry a species of viral RNA of 900,000 to 1,000,000 daltons. A viral RNA species of similar size is found early in the lytic cycle. Late in the viral lytic cycle, two prominent viral RNA species of about 600,000 and 900,000 daltons are seen. The larger lat...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.13.6.1263-1273.1974
更新日期：1974-06-01 00:00:00
abstract：:Hepatitis C virus (HCV) RNA genome replicates within the ribonucleoprotein (RNP) complex in the modified membranous structures extended from endoplasmic reticulum. A proteomic analysis of HCV RNP complexes revealed the association of oxysterol binding protein (OSBP) as one of the components of these complexes. OSBP in...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00958-09
更新日期：2009-09-01 00:00:00
abstract：:The relationship between NS protein phosphorylation and RNA polymerase activities was determined in nucleocapsids purified from vesicular stomatitis virus grown in BHK cells. Phosphate incorporation into endogenous NS protein under transcription conditions reached a maximum value of 0.06 mol/mol of NS within 20 to 30 ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.64.7.3259-3264.1990
更新日期：1990-07-01 00:00:00
abstract：:We report several biological activities of a synthetic peptide whose sequence contains the highly conserved region of feline leukemia virus transmembrane protein (TM) synthetically linked to another short TM-derived sequence particularly rich in polar positive residues. This 29-amino-acid peptide blocked [3H]thymidine...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.64.4.1429-1436.1990
更新日期：1990-04-01 00:00:00
abstract：:The structures of canine parvovirus (CPV) and feline parvovirus (FPV) complexed with antibody fragments from eight different neutralizing monoclonal antibodies were determined by cryo-electron microscopy (cryoEM) reconstruction to resolutions varying from 8.5 to 18 A. The crystal structure of one of the Fab molecules ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.02532-08
更新日期：2009-06-01 00:00:00
abstract：:During the 2009 H1N1 influenza virus pandemic (pdmH1N1) outbreak, it was found that most individuals lacked antibodies against the new pdmH1N1 virus, and only the elderly showed anti-hemagglutinin (anti-HA) antibodies that were cross-reactive with the new strains. Different studies have demonstrated that prior contact...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.05540-11
更新日期：2012-02-01 00:00:00
abstract：:The African swine fever virus protein A238L inhibits activation of NFAT transcription factor by binding calcineurin and inhibiting its phosphatase activity. NFAT controls the expression of many immunomodulatory proteins. Here we describe a 14-amino-acid region of A238L that is needed and sufficient for binding to calc...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/jvi.74.20.9412-9420.2000
更新日期：2000-10-01 00:00:00
abstract：:The expression of reporter genes driven by the same human elongation factor 1alpha (EF1alpha) promoter in murine leukemia virus (MLV)- and human immunodeficiency virus type 1 (HIV-1)-based vectors was studied in either transfected or virally transduced cells. The HIV-1 vectors consistently expressed 3 to 10 times high...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/jvi.76.14.7209-7219.2002
更新日期：2002-07-01 00:00:00
abstract：UNLABELLED:The ATR kinase has essential functions in maintenance of genome integrity in response to replication stress. ATR is recruited to RPA-coated single-stranded DNA at DNA damage sites via its interacting partner, ATRIP, which binds to the large subunit of RPA. ATR activation typically leads to activation of the ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.01412-14
更新日期：2014-09-01 00:00:00
abstract：:In polarized T cells, HIV-1 Gag localizes to a rear-end protrusion known as the uropod in a multimerization-dependent manner. Gag-laden uropods participate in formation of virological synapses, intercellular contact structures that play a key role in cell-to-cell HIV-1 transmission. Our previous observations suggest t...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00040-13
更新日期：2013-06-01 00:00:00
abstract：:In this study we report the complete sequence and genome organization of the serotype I feline coronavirus (FCoV) strain Black. Furthermore, a reverse genetic system was established for this FCoV strain by cloning a full-length cDNA copy into vaccinia virus. This clone served as basis for the generation of recombinant...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.02339-07
更新日期：2008-02-01 00:00:00
abstract：:There are no known RNA viruses that infect Archaea. Filling this gap in our knowledge of viruses will enhance our understanding of the relationships between RNA viruses from the three domains of cellular life and, in particular, could shed light on the origin of the enormous diversity of RNA viruses infecting eukaryot...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.07196-11
更新日期：2012-05-01 00:00:00
abstract：:In patients with impaired cell-mediated immune responses (e.g., lung transplant recipients and AIDS patients), cytomegalovirus (CMV) infection causes severe disease such as pneumonitis. However, although immunocompetency in the host can protect from CMV disease, the virus persists by evading the host immune defenses. ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.72.7.5535-5544.1998
更新日期：1998-07-01 00:00:00
abstract：:Adenoviral RNA sequences synthesized in nuclei isolated during the late phase of productive infection comprise, besides virus-associated, VA, RNA, five major species, ranging in size from approximately 13S to 55S. The latter RNA species is of the length predicted if the major transcriptional unit expressed during the ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.32.2.394-403.1979
更新日期：1979-11-01 00:00:00
abstract：:We observed two patterns of chemokine expression in the lungs of mice infected with murine gammaherpesvirus 68: peaks of chemokine expression correlated with or occurred after the peak of viral gene expression. Chemokine expression remained elevated through 29 days postinfection. ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/jvi.76.20.10518-10523.2002
更新日期：2002-10-01 00:00:00
abstract：:To clarify the role of core protein phosphorylation in pregenomic-RNA encapsidation of human and duck hepatitis B viruses (HBV and DHBV, respectively), we have examined the phosphorylation states of different forms of intracellular HBV core protein and the phenotypic effects of mutations in the phosphorylation sites o...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/jvi.74.10.4721-4728.2000
更新日期：2000-05-01 00:00:00
abstract：:Human trophoblast cells were permissively infected by human cytomegalovirus. The kinetics of viral immediate-early, early, and late gene expression was clearly delayed compared to that in fibroblasts. Productive infection was unequivocally proven by the detection of virion particles, infectious virus in trophoblast cu...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.72.9.7598-7602.1998
更新日期：1998-09-01 00:00:00
abstract：:In order to study retroviral variation, selection, and viral correlates of in vivo pathogenicity, we documented the evolution of feline leukemia virus (FeLV) variants in cats that died with thymic lymphoma after infection with molecularly cloned subgroup A FeLV. Using genomic DNA from cat necropsy samples, we employed...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.68.4.2458-2467.1994
更新日期：1994-04-01 00:00:00
abstract：:The adenovirus type 4 origins of replication are located at each end of the linear, protein-linked viral DNA molecule and consist of the terminal 18 bp of the viral genome. The sequence of the first 8 bp of the viral genome varies among different adenovirus serotypes, but the sequence from bp 9 to 18 is conserved in a...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.65.9.5037-5044.1991
更新日期：1991-09-01 00:00:00
